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Beethoven1770
Psychonaut



Registered: 05/17/15
Posts: 96
Last seen: 7 years, 4 months
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PF Tek Log: First Grow (UPDATED 6/1/15)
#21721326 - 05/25/15 03:12 PM (8 years, 8 months ago) |
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Hey, everyone! I am currently 11 days into my first grow and have decided to make a log detailing the adventure! I will recount my method for inoculating the jars and provide updates/pictures throughout the cultivation process. Enjoy!
Materials -Psilocybe Cubensis spore syringe -five canning jars (1/2 pint) -horticultural vermiculite -brown rice flour -water -mixing bowl -measuring cups -spatula -91% isopropyl alcohol -anti-bacterial wipes -Lysol spray -anti-bacterial soap -rubber gloves -surgical masks -reptile heating mat -pot with lid -lighter -aluminum foil -perlite -nails -clear plastic tub with lid
Day 0 (Inoculation) 1:45 PM- I sprayed down my work room with Lysol and wiped down the counter where the substrate mixture would take place using anti-bacterial wipes. I also washed my mixing bowl and spatula with antibacterial soap. 2:00 PM- I hammered four holes into each of the jars with a clean nail. I then prepared the substrate by adding 2 cups of vermiculite and 1 cup of water to the mixing bowl. I stirred this well with the spatula before adding 1 cup of brown rice flour. I continued to stir until it looked like the wet vermiculite was evenly coated with a layer of brown rice flour. I then divided the substrate among the five jars (without any packing down), filling each jar to 3/4 of an inch from the rim. I wiped the wet particles that got stuck around the rim away with a paper towel and poured dry vermiculite on top of the substrate. I screwed on the lids and folded a square of foil over the jar lids as tightly as possible. 2:10 PM- I placed the jars in my pot w/ lid with about 1" of water. I also put some extra jar lids in the bottom of the pot to keep the glass from touching it. I heated the water to a boil on my electric stove and left the jars in there for 90 minutes to sterilize before removing them to cool. 8:30 PM- I sprayed down my entire work room with lysol and wiped down all surfaces in the room with anti-bacterial wipes. The air-conditioning and fans were left off today to reduce air circulation. I put on clean clothes, rubber gloves, and a surgical mask. I then sprayed myself and the room with lysol again and waited five minutes for it to settle. I wiped down my gloves with rubbing alcohol and took the foil off the jar lids. I removed the cap from my spore syringe and flame sterilized it for 5 seconds. I injected 1 cc of spore solution into each jar (approximately .25 cc per hole), flame sterilizing the syringe after each jar. I put the cap back on the syringe and placed the jars in a dark cabinet with a reptile heating mat that keeps them at ~82 degrees F.
Days 1-3 no visible signs of growth.
Day 4 visible growth in one jar

Day 6 all jars showing growth!

Day 9 growth update

Day 11 growth update for today!
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Edited by Beethoven1770 (07/01/15 11:10 AM)
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LocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ



Registered: 04/17/15
Posts: 7,076
Loc: to the brain
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Those are some damn fine looking specimens you got there...good job.
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Q&A US vs. THEM The more I learn, the less I know.
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Beethoven1770
Psychonaut



Registered: 05/17/15
Posts: 96
Last seen: 7 years, 4 months
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Re: PF Tek Log: First Grow [Re: LocN9ne]
#21721710 - 05/25/15 04:50 PM (8 years, 8 months ago) |
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Thanks!
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Beethoven1770
Psychonaut



Registered: 05/17/15
Posts: 96
Last seen: 7 years, 4 months
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Oh, I almost forgot; this is the Tek I referenced the most http://www.shroomery.org/forums/showflat.php/Number/11585613/fpart/ Props to Shea25 for an awesome writeup!
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OneBigMushroom
Stranger

Registered: 05/16/15
Posts: 20
Last seen: 8 years, 7 months
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Looking good! My first grow took 10 days just to see germination because I probably didn't inoculate on the sides. I was gonna advise the wide mouth half pints for faster colonization but you seem fine without em lol. Good luck
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Beethoven1770
Psychonaut



Registered: 05/17/15
Posts: 96
Last seen: 7 years, 4 months
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Thanks! I wanted to get the wide mouth half pints too but I couldn't find them anywhere... I tried to inject the spores so that I could see solution running down the inside of the glass so I would see growth faster. I think the heating pad has a lot to do with the quick growth as well.
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Beethoven1770
Psychonaut



Registered: 05/17/15
Posts: 96
Last seen: 7 years, 4 months
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The jars are all 100% colonized! They will be birthed next Monday.
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fan5
Squatch

Registered: 09/16/11
Posts: 75
Last seen: 6 months, 3 days
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Looks awesome man!
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Beethoven1770
Psychonaut



Registered: 05/17/15
Posts: 96
Last seen: 7 years, 4 months
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Re: PF Tek Log: First Grow [Re: fan5]
#21757140 - 06/03/15 11:56 AM (8 years, 7 months ago) |
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Thanks!
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Myconin
Mushroom Ninja



Registered: 05/11/15
Posts: 308
Loc: The shadows...
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Normally, I'd advise against heating pads, as colonization is best done between 65F and 75F ime, but it's workin' out quite well for you my man 
Keep us updated, this looks like a great job so far!
-------------------- "No great mind has ever existed without a touch of madness" - Aristotle "I have just three things to teach: Simplicity, Patience, Compassion. These three are your greatest treasures." - Lao Tzu "You've just gotta keep on keepin' on, man. You can't have 'no' in your heart" - Joe Dirt ThirtyCigarettes said: "All I know is every other thread I see in the Cultivation forum goes like this: QUESTION > ANSWER > DIFFERENT ANSWER > ARGUE > TC COMES AND CLEARS IT UP"
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Beethoven1770
Psychonaut



Registered: 05/17/15
Posts: 96
Last seen: 7 years, 4 months
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Re: PF Tek Log: First Grow [Re: Myconin]
#21758245 - 06/03/15 05:21 PM (8 years, 7 months ago) |
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Thanks dude! I've heard that room temp works great, but that keeping it in the low 80's can speed up colonization. I'm making some wbs jars right now, wish me luck!
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Beethoven1770
Psychonaut



Registered: 05/17/15
Posts: 96
Last seen: 7 years, 4 months
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Here's a pic of day 2 in the SGFC:

I birthed, rinsed, and dunked the cakes on sunday night. I took them out the next morning, rinsed them again, and rolled them in vermiculite before putting them in the SGFC.
Also in the picture are my five WBS jars that I inoculated on Thursday. They are all showing growth!
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Beethoven1770
Psychonaut



Registered: 05/17/15
Posts: 96
Last seen: 7 years, 4 months
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After 10 days in the FC the cakes have finally started pinning!
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ShroominMe
Stranger

Registered: 05/03/15
Posts: 525
Last seen: 8 years, 3 months
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Quote:
Beethoven1770 said: After 10 days in the FC the cakes have finally started pinning!
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Beethoven1770
Psychonaut



Registered: 05/17/15
Posts: 96
Last seen: 7 years, 4 months
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Re: PF Tek Log: First Grow [Re: ShroominMe]
#21832975 - 06/20/15 04:31 PM (8 years, 7 months ago) |
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I harvested the fruits from one of the cakes yesterday!!
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Grim767
Traveler of the Abyss



Registered: 05/30/15
Posts: 225
Loc: The Abyss
Last seen: 7 years, 3 months
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Looks good man congrats
-------------------- Trade List I won't bow to something that I've never seen, Can't believe in something that doesn't believe in me, I'm not blood of your blood, I'm no son of your god, I have no faith in your faith, Still I find salvation.
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Beethoven1770
Psychonaut



Registered: 05/17/15
Posts: 96
Last seen: 7 years, 4 months
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Re: PF Tek Log: First Grow [Re: Grim767]
#21833351 - 06/20/15 06:19 PM (8 years, 7 months ago) |
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Dude we have the same picture, that's crazy!
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Beethoven1770
Psychonaut



Registered: 05/17/15
Posts: 96
Last seen: 7 years, 4 months
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Hey guys, I know it's been a while since I've posted any updates, so here are some pics!
This is the second flush for this cake. some of the others are already on their third. The veils tore over night and got spores everywhere, but oh well.

Here are some batches drying out in front of the fan. I didn't get pictures of all of them, but I've pulled about 30 grams dried from the nine cakes so far and they are still flushing!! I'm so happy 


Thanks for checking out my first grow! Comments and suggestions are welcome
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