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Offlineericfloyd64
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Pan cambo goliath incubation temp
    #21721006 - 05/25/15 01:48 PM (8 years, 8 months ago)

Well with my first pan cyan Hawaiian attempt a complete failure because I used shitty Walmart humus and manure mix, I tried some pan cambo goliaths using waylitjims tek. I bought the Scott's humus and manure mix he suggested and made some container similar to his, using a 1:1:1:1 mix of the manure mix, verm, BRF, and water, and knocked them up using a syringe. But with over a week maybe almost two, there is no mycelium growth whatsoever. I was wondering if my temps were wrong, I used had it around 86 but it spiked to 93. I heard they need warmer temps so I wasn't too concerned. If these don't work I'm going out to my friends farm and get some longhorn shit


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Offlinespacechildo
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Re: Pan cambo goliath incubation temp [Re: ericfloyd64]
    #21721027 - 05/25/15 01:54 PM (8 years, 8 months ago)

that's way hot IMO. be patient and let the spores germinate, try them directly on grain or on agar if you feel like,
then spawn to the manure mix.


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Offlineericfloyd64
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Re: Pan cambo goliath incubation temp [Re: spacechildo]
    #21721202 - 05/25/15 02:45 PM (8 years, 8 months ago)

What should the temperature be? Im about to order some agar plates that are pre made so I will just put the last .5 cc of the goliath spores on there, how should I go about it? Im on my first attempt with grains using some cube spores, I'm still waiting for those to start colonizing


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Re: Pan cambo goliath incubation temp [Re: ericfloyd64]
    #21721226 - 05/25/15 02:49 PM (8 years, 8 months ago)

max 80. 75 is good IMO.
just put a drop on agar, 0,5cc is too much. pre-made agar plates are rarely worth the price and hassle.


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Offlineericfloyd64
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Re: Pan cambo goliath incubation temp [Re: spacechildo]
    #21721775 - 05/25/15 05:09 PM (8 years, 8 months ago)

Why are they a hassle? I feel like they do everything for you? And where do I order agar from? And do you think the goliaths are done for? I also have a bunch of golden teacher jars in there that I made with a spore print that I made which I have done before and yet these jars also show no mycelium growth


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Offlinespacechildo
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Re: Pan cambo goliath incubation temp [Re: ericfloyd64]
    #21723480 - 05/26/15 03:17 AM (8 years, 8 months ago)

I buy agar locally. most of the sponsors should carry some pre-mixed agar at least. I like mixing my own.
its a hassle because they're pre-contamed. I just dont see shipping pre-poured plates through the mail as a good thing.


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Offlinehamloaf
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Re: Pan cambo goliath incubation temp [Re: ericfloyd64]
    #21723513 - 05/26/15 03:49 AM (8 years, 8 months ago)

Quote:

ericfloyd64 said:
Well with my first pan cyan Hawaiian attempt a complete failure because I used shitty Walmart humus and manure mix, I tried some pan cambo goliaths using waylitjims tek. I bought the Scott's humus and manure mix he suggested and made some container similar to his, using a 1:1:1:1 mix of the manure mix, verm, BRF, and water, and knocked them up using a syringe. But with over a week maybe almost two, there is no mycelium growth whatsoever. I was wondering if my temps were wrong, I used had it around 86 but it spiked to 93. I heard they need warmer temps so I wasn't too concerned. If these don't work I'm going out to my friends farm and get some longhorn shit



Assumiing there are no issues with your sterilization methods & procedures, for spore germination, and mycelial colonization of grains 70-75F is optimal.  It's not until fruit body formation do the Pans prefer a slightly higher temperature range (80-85F), than tropical cubensis varieties.  Fruiting Pans in the 70-75F temperature range isn't a deal breaker though.  Begin again, and keep your temps in the 70-75F range for germination, and colonization.


--------------------

   
How I Get Stuff done. - My Reference Guide. - My Grows.


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Offlineericfloyd64
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Re: Pan cambo goliath incubation temp [Re: hamloaf]
    #21724395 - 05/26/15 10:51 AM (8 years, 8 months ago)

Will do. So where do I buy agar locally and how do I prepare it?


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Re: Pan cambo goliath incubation temp [Re: ericfloyd64]
    #21724411 - 05/26/15 10:58 AM (8 years, 8 months ago)

asian markets/thai stores, just add some nutes and hot water like this http://www.shroomery.org/forums/showflat.php/Number/19208976


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Offlineericfloyd64
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Re: Pan cambo goliath incubation temp [Re: spacechildo]
    #21724556 - 05/26/15 11:51 AM (8 years, 8 months ago)

Thanks I'm about to take a look at it right now, seems very detailed which is exactly what I need. You have some other great links in your signature!


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Offlineericfloyd64
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Re: Pan cambo goliath incubation temp [Re: ericfloyd64]
    #21724592 - 05/26/15 12:01 PM (8 years, 8 months ago)

How much spore solution should I pour onto one of the glad mini rounds?


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Offlinespacechildo
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Re: Pan cambo goliath incubation temp [Re: ericfloyd64]
    #21724597 - 05/26/15 12:03 PM (8 years, 8 months ago)

1 drop. its easier to use a print if you have and not a syringe.


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Offlineericfloyd64
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Re: Pan cambo goliath incubation temp [Re: spacechildo]
    #21725193 - 05/26/15 03:02 PM (8 years, 8 months ago)

I have a few prints of golden teachers and ecuadorians but these goliaths. How long does the agar take and then how do I transfer it to inoculate some jars?


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Re: Pan cambo goliath incubation temp [Re: ericfloyd64]
    #21725242 - 05/26/15 03:17 PM (8 years, 8 months ago)

http://www.shroomery.org/forums/showflat.php/Number/18430998
you just need a sab, a blade and a flame.


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Offlineericfloyd64
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Re: Pan cambo goliath incubation temp [Re: spacechildo]
    #21725314 - 05/26/15 03:40 PM (8 years, 8 months ago)

Thanks again man. So I read both of those articles you sent me I'm still a little confused on how you put the culture into jars such as grains or brf/manure jars


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Re: Pan cambo goliath incubation temp [Re: ericfloyd64]
    #21725323 - 05/26/15 03:43 PM (8 years, 8 months ago)

brf jars isn't practical to add agar wedges to.

for a grain jar either do the tiger drop described/linked to in the pastwhyte easy agar tek
or just cut a wedge with a sterilized blade and drop into the grain jar in a SAB.

its really easy actually, once you try it you'll see! :super:


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Offlineericfloyd64
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Re: Pan cambo goliath incubation temp [Re: ericfloyd64]
    #21725479 - 05/26/15 04:29 PM (8 years, 8 months ago)

Great thanks a lot man I really appreciate it! I've been meaning to get into agar but it all seemed too foreign.


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Re: Pan cambo goliath incubation temp [Re: spacechildo]
    #21725497 - 05/26/15 04:34 PM (8 years, 8 months ago)

Just try regular old manure with brf It works great. Go witha 1/4 cup manure thats soaked to field capacity added to the regular pf tek recipe. I have no experience with baggged brands but even regular BRF for growing out mycellium and spawned to thin trays of straw or horse manure works . Tried and true.

Waylit was one of those cultivators that could work with ANY substrate and get it to work perfectly esp working with the isolates he had. I had the pleasure of picking his brain when I first started in the OMC in 2007 . Talented guy for sure. Good luck


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InvisibleTomandjerry58
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Re: Pan cambo goliath incubation temp [Re: ericfloyd64]
    #21725499 - 05/26/15 04:34 PM (8 years, 8 months ago)

Good luck on getting this going and you will most likely need the agar like everyone else is saying.

Great mushroom tho...active at 300 mg:thumbup:


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Re: Pan cambo goliath incubation temp [Re: Tomandjerry58]
    #21725509 - 05/26/15 04:38 PM (8 years, 8 months ago)

Ive never used agar a day in my life... Id like to but working in a tiny closed space trying to be sterile and pour plates doesn't sound fun with my ADD.  You can do many things with good genetics and learning how to cultivate. It just takes practice.

I grew at 68 70° throughout the whole run with pans. They went a little slower than summertime but it worked pretty well. If I dont see growth in 14 days, toss em and start another batch. Make sure to remember to shake your syringe. Vendor syringes sometimes have caked spores that have germinated so if not shaken youll get the whole clump in two jars.


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Edited by mycomaniac1402 (05/26/15 04:40 PM)


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InvisibleTomandjerry58
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Re: Pan cambo goliath incubation temp [Re: mycomaniac1402]
    #21725554 - 05/26/15 04:51 PM (8 years, 8 months ago)

Well def not all the time necessary but its actually pretty easy to work with agar and almost enjoyable compared to working with jars substrates...etc. Also once you get a few good strains going. Plates are always ready so you can innoc anytime...not to mention the strains have been worked with so you are getting he best of the best once it goes into jars.

A lot easier if you just slow down and take your time....which you can do in a sab. Be smart about the moves you make....

Pasty plates are no pour:thumbup:


Edited by Tomandjerry58 (05/26/15 04:52 PM)


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Offlineericfloyd64
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Re: Pan cambo goliath incubation temp [Re: Tomandjerry58]
    #21726191 - 05/26/15 07:40 PM (8 years, 8 months ago)

Thanks guys I have three homemade agar containers in the PC right now. Should the mix in the container be dry when I drop spore solution onto it? And thanks mcomaniac, I will try that once it stops raining here in Texas, we have been having crazy floods which have stalled me from getting some dry longhorn manure from my friends farm


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