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Straya
Wot?!


Registered: 03/04/12
Posts: 705
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Last seen: 4 years, 8 months
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Straya's Spore Slurry Tek
#21720134 - 05/25/15 09:24 AM (8 years, 8 months ago) |
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In the wonderful book Growing gourmet and medicinal mushrooms by Paul Stamets, he briefly speaks about a spore-mass slurry or spore mass inoculation technique that he would like to further investigate.
I thought I would do a little tek for how I've done mine and hopefully will help others to expand their mycological landscapes.
Required: A weed sprayer bottle (Local hardware store) Molasses - found in local grocery store near golden syrup Salt A few litres of water Fresh specimens 20L bucket Sex lubricant (optional)
This spore slurry was done with Psilocybe subaeruginosa specimens which were freshly picked.

I bought a weed sprayer bottle (5L) from my local hardware store and filled it with tap water.
I then removed the stems from the mushrooms that were to be placed in the bottle.

Now it is time to prepare the water solution for the specimens. In 'Growing gourmet and medicinal mushrooms' Paul Stamets says to add a few grams of salt to the solution to inhibit bacteria from growing, which won't substantially affect the viability of the spores.

Next I added some Molasses which I just bought from the store, Stamets recommends to put 50ml into the solution. The Molasses is added which stimulates the spores into a frenzied germination. After four hours of soaking he recommends to remove the mushroom caps, they should have released thousands of spores by now. I left mine for 24 hours.

In this process I did not add sex lube to the solution as I forgot, seeing as RogerRabbit recommends it in his videos to keep the spores from sticking to the inside of the syringes it may be worthwhile to add a bit of lube to the solution so the spores don't stick to the inside of the sprayer, I will be adding it next time around.
Once the ingredients essential to our solution are mixed together I gave the container a good shake to mix in the Molasses. Following this I then placed the mushrooms in the solution.

After adding the mushrooms to the solution I put on the lid and gave the container a gentle shake to move the mushrooms around the water and stop them from floating on the top.


It is mentioned by Stamets that you can increase the volume of your solution by up to 10 times. I've started this solution in a small 5 litre sprayer so I will be transferring it to a 20 litre bucket and adding an additional 15 litres of water to increase my solution after 48 hours of soaking the spores.
There you have it. Now I am going to spray every fucking woodchip bed within 2 hours of my home after the next decent rainfall so the ground is moist and will help the spores to germinate, this is just personal preference my theory is that if I do it when it's hot and dry the spores will most likely struggle to germinate and as they seem to have so much success whilst hydrated I would like to apply them to the areas when it is wet to help them grow. Hopefully will see some action next year.
Quote:
Juiceh said: You might have better luck if you spray right before a rain. The rain will drive the slurry deeper into the beds.
As the impatient person that I am, I cannot wait 12 months to see if this will work. I'm going to take a few test samples out of the final solution with a pipette and place them on agar. Hopefully within a week or two i'll have a good indication of whether this works or not.
Thanks for reading.
Straya
Edited by Straya (05/25/15 11:04 AM)
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PhosCap
Gratuitous Heavenly Grace



Registered: 06/09/10
Posts: 975
Loc: Tartary
Last seen: 8 months, 14 days
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Re: Straya's Spore Slurry Tek [Re: Straya]
#21720176 - 05/25/15 09:40 AM (8 years, 8 months ago) |
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awesome! This is now on my project list.  I wonder if you can put a screen mesh over the top and put mush caps and leave there for 24 hrs instead of dropping caps into water.
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Juiceh
Dabbing All Day



Registered: 09/25/12
Posts: 3,208
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Re: Straya's Spore Slurry Tek [Re: Straya]
#21720178 - 05/25/15 09:41 AM (8 years, 8 months ago) |
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Nice. I been thinking about doing this with morels. You might have better luck if you spray right before a rain. The rain will drive the slurry deeper into the beds. As for checking the slurry on agar. It probably won't be clean, I'm betting you will get mycelial growth along with some contams. But when you apply this outside I don't think it will matter much and the mushroom mycelium will do just fine.
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Straya
Wot?!


Registered: 03/04/12
Posts: 705
Loc:
Last seen: 4 years, 8 months
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Re: Straya's Spore Slurry Tek [Re: Juiceh]
#21720188 - 05/25/15 09:43 AM (8 years, 8 months ago) |
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Quote:
phosi said: awesome! This is now on my project list.  I wonder if you can put a screen mesh over the top and put mush caps and leave there for 24 hrs instead of dropping caps into water.

Yeah that sounds alright however then it wouldn't be exposed to the molasses which allegedly makes the spores go ape shit but sounds cool 
Quote:
Juiceh said: Nice. I been thinking about doing this with morels. You might have better luck if you spray right before a rain. The rain will drive the slurry deeper into the beds. As for checking the slurry on agar. It probably won't be clean, I'm betting you will get mycelial growth along with some contams. But when you apply this outside I don't think it will matter much and the mushroom mycelium will do just fine.
You sir are a thinker! Will do 
Just want to see if I can get some mycelium growing on the agar, it's not a problem to clean it up just want to see if there's a decent amount in the test samples
Edited by Straya (05/25/15 11:02 AM)
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Fantastic Mr. Fox
Grass Seed Connoisseur

Registered: 02/12/15
Posts: 3,225
Loc:
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Re: Straya's Spore Slurry Tek [Re: Straya]
#21720770 - 05/25/15 12:45 PM (8 years, 8 months ago) |
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-------------------- Giving is all we have, for we're just grateful to be alive

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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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it might be worth trying to make some wood chip spawn jars too. I wouldn't try making grain spawn animals and bugs will just eat that. you can germinate on agar, clean up and then inoculate sterilized sawdust or try pasteurized sawdust. the more you use the better chance for results. whatever is the best head start you can give them. it's something to fuck around with in your spare time.
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Fantastic Mr. Fox
Grass Seed Connoisseur

Registered: 02/12/15
Posts: 3,225
Loc:
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Re: Straya's Spore Slurry Tek [Re: bodhisatta]
#21720786 - 05/25/15 12:49 PM (8 years, 8 months ago) |
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Quote:
bodhisatta said: it might be worth trying to make some wood chip spawn jars too. I wouldn't try making grain spawn animals and bugs will just eat that. you can germinate on agar, clean up and then inoculate sterilized sawdust or try pasteurized sawdust. the more you use the better chance for results. whatever is the best head start you can give them. it's something to fuck around with in your spare time.
QFT
-------------------- Giving is all we have, for we're just grateful to be alive

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Milk Man
Here to Fuck Your Wife

Registered: 05/15/15
Posts: 93
Last seen: 8 years, 7 months
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nature scapes
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Straya
Wot?!


Registered: 03/04/12
Posts: 705
Loc:
Last seen: 4 years, 8 months
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Re: Straya's Spore Slurry Tek [Re: bodhisatta]
#21723916 - 05/26/15 07:55 AM (8 years, 8 months ago) |
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Quote:
bodhisatta said: it might be worth trying to make some wood chip spawn jars too. I wouldn't try making grain spawn animals and bugs will just eat that. you can germinate on agar, clean up and then inoculate sterilized sawdust or try pasteurized sawdust. the more you use the better chance for results. whatever is the best head start you can give them. it's something to fuck around with in your spare time.
I bought a small garden bed the other day to assemble and i'm going to fill with wood shavings and wood chips to start a small trial patch in my yard. Hopefully if it takes I will then break it up and distribute also.
Just hoping to get some spores out there and new patches going. I'm new to agar but would like to get some nice clean specimens going and wouldn't hesitate to go add some samples to the wild. Thanks for the info about the grain, i'll spawn it to woodchips and spread from there aswell as another side experiment
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