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RPW
Entheogen Cultivator


Registered: 03/01/12
Posts: 265
Loc: Nomadic
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Clone (No Further Isolation) vs. Multispore
#21719815 - 05/25/15 06:22 AM (8 years, 8 months ago) |
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If I were to take a tissue culture from a multispore grow and placed it on agar, the result could be multiple strains, and would sector. If I selected the tissue culture from a good looking, fast pinning mushroom, these substrains although not an isolate, are still showing good potential, at least to my knowledge. Would there be any benefit from using this clone without any further isolating vs. just starting with multispore?
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Looking to trade stamps for: ATL#7, Tampanensis, and Mexicana A-strain prints/syringes Pan. Cyanescens (Especially RDU) prints/syringes Please PM if you have any of these for trade.
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 19 hours
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Re: Clone (No Further Isolation) vs. Multispore [Re: RPW]
#21719914 - 05/25/15 07:16 AM (8 years, 8 months ago) |
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Quote:
RPW said: If I were to take a tissue culture from a multispore grow and placed it on agar, the result could be multiple strains, and would sector. If I selected the tissue culture from a good looking, fast pinning mushroom, these substrains although not an isolate, are still showing good potential, at least to my knowledge. Would there be any benefit from using this clone without any further isolating vs. just starting with multispore?
Yes there is a benefit.....you have good looking fast pinning genetics which have already germinated vs just a random crapshoot of spores that haven't......there is your benefit.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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Mr. Alien
I will abduct andprobe your anus



Registered: 01/14/14
Posts: 6,290
Loc: Star Wars Galaxy
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Re: Clone (No Further Isolation) vs. Multispore [Re: PussyFart]
#21719978 - 05/25/15 07:51 AM (8 years, 8 months ago) |
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Listen to uncle pussyfart
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RPW
Entheogen Cultivator


Registered: 03/01/12
Posts: 265
Loc: Nomadic
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Re: Clone (No Further Isolation) vs. Multispore [Re: PussyFart]
#21721630 - 05/25/15 04:26 PM (8 years, 8 months ago) |
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Alright, that's what I figured. If I wanted to keep a master slant for the clone, where would I cut into the agar to ensure I get all sectors? Just cut out a square from the middle?
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Looking to trade stamps for: ATL#7, Tampanensis, and Mexicana A-strain prints/syringes Pan. Cyanescens (Especially RDU) prints/syringes Please PM if you have any of these for trade.
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 10 hours
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Re: Clone (No Further Isolation) vs. Multispore [Re: RPW]
#21722152 - 05/25/15 07:11 PM (8 years, 8 months ago) |
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Quote:
RPW said: Alright, that's what I figured. If I wanted to keep a master slant for the clone, where would I cut into the agar to ensure I get all sectors? Just cut out a square from the middle?
You probably arent going to be able to get all of them, youll cut some out eventually from just moving the culture around so I just dont really worry about it too much and just take from the best looking spot on the edge... probably arent going to cut so many out that it makes a huge difference
When I find a clone that I like enough to slant what Ive been doing is isolating the sectors and fruiting them, I find theyre usually pretty similar anyways so I just take the best one and save that. It kinda stabalizes the clone, its a little bit of work though.
See what someone with more experience says tho
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 Amateur Mycologists United AMU Q&A
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RPW
Entheogen Cultivator


Registered: 03/01/12
Posts: 265
Loc: Nomadic
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Re: Clone (No Further Isolation) vs. Multispore [Re: mushpunx]
#21722774 - 05/25/15 09:37 PM (8 years, 8 months ago) |
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Well I planned on using a clone culture to make a master grain jar, and then G2G with that while I work on isolating from the clone. Would my best bet be to use the center to create the master grain jar, and use the outside sectoring portions to further isolate? I appreciate all the help everyone!
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Looking to trade stamps for: ATL#7, Tampanensis, and Mexicana A-strain prints/syringes Pan. Cyanescens (Especially RDU) prints/syringes Please PM if you have any of these for trade.
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 10 hours
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Re: Clone (No Further Isolation) vs. Multispore [Re: RPW]
#21723620 - 05/26/15 05:17 AM (8 years, 8 months ago) |
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To further work on the clone just take a transfer from the best looking spot on the edge, leading edge.
When you inoculate a grain jar you use a wedge, like a slice of pizza. Ill take 3 wedges from a full platr but everyome has a preference
I mean you can take from whereever you want on the plate to inoculate but most people do wedge
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 Amateur Mycologists United AMU Q&A
Edited by mushpunx (05/26/15 05:18 AM)
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Dhearic


Registered: 01/24/14
Posts: 846
Loc: Neverland
Last seen: 9 months, 4 days
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Re: Clone (No Further Isolation) vs. Multispore [Re: mushpunx]
#21723870 - 05/26/15 07:41 AM (8 years, 8 months ago) |
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Quote:
mushpunx said: To further work on the clone just take a transfer from the best looking spot on the edge, leading edge.
When you inoculate a grain jar you use a wedge, like a slice of pizza. Ill take 3 wedges from a full platr but everyome has a preference
I mean you can take from whereever you want on the plate to inoculate but most people do wedge 

Unless you have a million extra plates laying around and feel like being wasteful. Then you can be like me and take a fully colonized plate, cut it into 3-4 pieces, and put them all into one master jar lol
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Credit where credit is due.
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RPW
Entheogen Cultivator


Registered: 03/01/12
Posts: 265
Loc: Nomadic
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Re: Clone (No Further Isolation) vs. Multispore [Re: mushpunx]
#21726506 - 05/26/15 08:51 PM (8 years, 8 months ago) |
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Quote:
mushpunx said: To further work on the clone just take a transfer from the best looking spot on the edge, leading edge.
When you inoculate a grain jar you use a wedge, like a slice of pizza. Ill take 3 wedges from a full platr but everyome has a preference
I mean you can take from whereever you want on the plate to inoculate but most people do wedge 
Yea, there's a lot of information on how to go about isolating, but not on what to do if you're after multiple sectors. I'll probably just do what I said in my previous post.
On an unrelated noted, I picked up 25lbs of Rye Berries today, and realized as I was leaving there was a garden shop right next door. I went inside, and sure enough they had garden gypsum. Couldn't be happier that I now have a local source of both 20 feet away from each other 
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Looking to trade stamps for: ATL#7, Tampanensis, and Mexicana A-strain prints/syringes Pan. Cyanescens (Especially RDU) prints/syringes Please PM if you have any of these for trade.
Edited by RPW (05/26/15 08:52 PM)
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