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Pagliacci
Stranger
Registered: 05/24/15
Posts: 64
Last seen: 7 years, 2 months
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Can alcohol in the air of a glovebox kill spores?
#21717116 - 05/24/15 11:00 AM (8 years, 8 months ago) |
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Heard a joke once. Man goes to doctor. Says his first WBS tek worked great using a fume hood, autoclave, and third party spore print. His second attempt at WBS jar inoculation never took after 10+ days in a tub in tub at 79ยบ, but this was done at home with a AA915 PC, glovebox, and personal spore print from the first harvest. He then tried an LC tek with another personal spore print which is now at day 8 with no growth either. He is worried that spraying down inside the closed glovebox and then making spore prints is too much alcohol in the air for the spores to survive since every other variable he can think of was maintained. Jars used are silicone self healing with a double layer tyvek FAE hole.
-------------------- Man bursts into tears. Says, "but doctor...I am Pagliacci."
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Can alcohol in the air of a glovebox kill spores? [Re: Pagliacci]
#21717274 - 05/24/15 11:58 AM (8 years, 8 months ago) |
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Nice twist on the whole SWIM shtick. But if that was the reason then I would never have pulled off a grow yet. That is most likely not the vector.
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Rosen_Rot
Learning



Registered: 12/06/14
Posts: 1,225
Loc: Goa
Last seen: 11 months, 22 days
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Re: Can alcohol in the air of a glovebox kill spores? [Re: Pastywhyte]
#21717363 - 05/24/15 12:37 PM (8 years, 8 months ago) |
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Ahaha I was thinking of that myself, when is the punchline coming in?
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"The internet has one rule; use or be used" - Bjeldiablo ''there is no loneliness, only moments where contentment is fleeting'' SBJs "The Basics" 3iRiS9 "Cirque du freak" B+ BONANZA
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grainbrain
Tribalistic

Registered: 05/11/11
Posts: 2,626
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Re: Can alcohol in the air of a glovebox kill spores? [Re: Pastywhyte]
#21717365 - 05/24/15 12:37 PM (8 years, 8 months ago) |
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Spore to LC is always a gamble.
-------------------- Stop cold shocking your mycelium! Hot Spawn - Get It On 
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StygianKnight
A Mushroom

Registered: 03/12/12
Posts: 2,717
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Re: Can alcohol in the air of a glovebox kill spores? [Re: grainbrain]
#21717374 - 05/24/15 12:40 PM (8 years, 8 months ago) |
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Sure can if you flame your equipment inside the box as well.
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Rosen_Rot
Learning



Registered: 12/06/14
Posts: 1,225
Loc: Goa
Last seen: 11 months, 22 days
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Re: Can alcohol in the air of a glovebox kill spores? [Re: StygianKnight]
#21717377 - 05/24/15 12:43 PM (8 years, 8 months ago) |
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generally what will happen
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"The internet has one rule; use or be used" - Bjeldiablo ''there is no loneliness, only moments where contentment is fleeting'' SBJs "The Basics" 3iRiS9 "Cirque du freak" B+ BONANZA
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Can alcohol in the air of a glovebox kill spores? [Re: StygianKnight]
#21717387 - 05/24/15 12:45 PM (8 years, 8 months ago) |
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The alcohol will dissipate long before damage is done to the spores. Takes about 45 min depending on how sealed the box is. Flaming tools in that time will make a boom but given we don't make prints in less than 45 min its likely that even if most did die off due to alcohol vapors at least a few billion would survive.
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