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LumpyNutz
Bad fisH <*))))<



Registered: 07/14/14
Posts: 712
Loc: Stuck in this dream
Last seen: 6 years, 5 months
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Black MOLD! - Bad print/bad syringe?
#21716923 - 05/24/15 09:23 AM (8 years, 8 months ago) |
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This might be a hard question to diagnose but here it is: I made a spore print from a B+ cap a couple weeks ago and the next day made a spore syringe in a SAB. Then a couple days later noc'd up 12 BRF jars and within a few days noticed ALL of them were growing black mold under the inoculation points along with small spots of mycelial growth. I only used about a quarter of the print and was considering making a new syringe with it. BUT I dont know weather its the print that somehow got contaminated or if I fucked up somehow when making the syringe. Im just wondering if its worth trying again with the same print or should I just toss it and make a new print? And can prints get contaminated easily?
Heres what these sick puppies look like btw =(

Thanks for any advise
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Black MOLD! - Bad print/bad syringe? [Re: LumpyNutz]
#21716945 - 05/24/15 09:39 AM (8 years, 8 months ago) |
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Probably the print. Don't take another quarter/half. You're about to get bombarded telling you to try agar and I'm 1 of them. Just scrape a tiny amount of spores off with an inoculation loop and rub the spores on the surface of the agar plate. If you get black mold just cut the healthy mycelium away from it and voila clean inoculant
You can then take a clean wedge of mycelium and make a liquid culture out of it. You'd have a lot higher success getting a clean liquid culture syringe over a clean spore syringe this way.
Edited by Mad Season (05/24/15 09:40 AM)
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LumpyNutz
Bad fisH <*))))<



Registered: 07/14/14
Posts: 712
Loc: Stuck in this dream
Last seen: 6 years, 5 months
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Re: Black MOLD! - Bad print/bad syringe? [Re: Mad Season]
#21717151 - 05/24/15 11:13 AM (8 years, 8 months ago) |
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Quote:
Mad Season said: Probably the print. Don't take another quarter/half. You're about to get bombarded telling you to try agar and I'm 1 of them. Just scrape a tiny amount of spores off with an inoculation loop and rub the spores on the surface of the agar plate. If you get black mold just cut the healthy mycelium away from it and voila clean inoculant
You can then take a clean wedge of mycelium and make a liquid culture out of it. You'd have a lot higher success getting a clean liquid culture syringe over a clean spore syringe this way.
Thanks for the reply! I havnt done much research on LC is it pretty simple to make compared to a spore syringe? If u know a good tek that would be greatly appreciated. Also where can I buy some agar plates? Some type of lab/chemistry shop I would assume? Or gotta order em online?
-------------------- This is uh... no offence... but you are a robot aren't you?
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Black MOLD! - Bad print/bad syringe? [Re: LumpyNutz]
#21717188 - 05/24/15 11:20 AM (8 years, 8 months ago) |
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PantsR4squares
Adventurer



Registered: 11/02/13
Posts: 170
Loc: In the woods
Last seen: 21 hours, 11 minutes
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Re: Black MOLD! - Bad print/bad syringe? [Re: Mad Season]
#21737430 - 05/29/15 04:01 PM (8 years, 7 months ago) |
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I just had some black mold on agar
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Black MOLD! - Bad print/bad syringe? [Re: PantsR4squares]
#21737437 - 05/29/15 04:03 PM (8 years, 7 months ago) |
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if you can't make a clean syringe LC is the last thing you want to try to make
stick with agar until you can do things clean then think about LC
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