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OfflineLumpyNutz
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Black MOLD! - Bad print/bad syringe?
    #21716923 - 05/24/15 09:23 AM (8 years, 8 months ago)

This might be a hard question to diagnose but here it is:
I made a spore print from a B+ cap a couple weeks ago and the next day made a spore syringe in a SAB. Then a couple days later noc'd up 12 BRF jars and within a few days noticed ALL of them were growing black mold under the inoculation points along with small spots of mycelial growth.
I only used about a quarter of the print and was considering making a new syringe with it. BUT I dont know weather its the print that somehow got contaminated or if I fucked up somehow when making the syringe.
Im just wondering if its worth trying again with the same print or should I just toss it and make a new print? And can prints get contaminated easily?

Heres what these sick puppies look like btw =(


Thanks for any advise


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InvisibleMad Season
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Re: Black MOLD! - Bad print/bad syringe? [Re: LumpyNutz]
    #21716945 - 05/24/15 09:39 AM (8 years, 8 months ago)

Probably the print. Don't take another quarter/half. You're about to get bombarded telling you to try agar and I'm 1 of them. Just scrape a tiny amount of spores off with an inoculation loop and rub the spores on the surface of the agar plate. If you get black mold just cut the healthy mycelium away from it and voila clean inoculant

You can then take a clean wedge of mycelium and make a liquid culture out of it. You'd have a lot higher success getting a clean liquid culture syringe over a clean spore syringe this way.


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Edited by Mad Season (05/24/15 09:40 AM)


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OfflineLumpyNutz
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Re: Black MOLD! - Bad print/bad syringe? [Re: Mad Season]
    #21717151 - 05/24/15 11:13 AM (8 years, 8 months ago)

Quote:

Mad Season said:
Probably the print. Don't take another quarter/half. You're about to get bombarded telling you to try agar and I'm 1 of them. Just scrape a tiny amount of spores off with an inoculation loop and rub the spores on the surface of the agar plate. If you get black mold just cut the healthy mycelium away from it and voila clean inoculant

You can then take a clean wedge of mycelium and make a liquid culture out of it. You'd have a lot higher success getting a clean liquid culture syringe over a clean spore syringe this way.




Thanks for the reply! I havnt done much research on LC is it pretty simple to make compared to a spore syringe? If u know a good tek that would be greatly appreciated.
Also where can I buy some agar plates? Some type of lab/chemistry shop I would assume? Or gotta order em online?


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InvisibleMad Season
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Re: Black MOLD! - Bad print/bad syringe? [Re: LumpyNutz]
    #21717188 - 05/24/15 11:20 AM (8 years, 8 months ago)

http://www.shroomery.org/forums/showflat.php/Number/19208976/fpart/1/vc/1

http://www.shroomery.org/forums/showflat.php/Number/10446142

This is a step in the right direction but as usual you're going to need to research a lot more than this


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contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
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OfflinePantsR4squares
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Re: Black MOLD! - Bad print/bad syringe? [Re: Mad Season]
    #21737430 - 05/29/15 04:01 PM (8 years, 7 months ago)

I just had some black mold on agar  :strokebeard2:


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InvisiblebodhisattaMDiscordReddit
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Re: Black MOLD! - Bad print/bad syringe? [Re: PantsR4squares]
    #21737437 - 05/29/15 04:03 PM (8 years, 7 months ago)

if you can't make a clean syringe LC is the last thing you want to try to make

stick with agar until you can do things clean then think about LC


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