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Offlineslartibartfast
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Registered: 05/13/08
Posts: 203
Last seen: 3 years, 11 months
hericium americanum spawn issues
    #21713760 - 05/23/15 10:48 AM (8 years, 8 months ago)

Greetings
I have been having troubles getting hericium americanum to colonize 1.9 Q mason jars and am at a loss to explain it. I grow on whole wheat or barley with a little bit of WBS and have no problems getting full colonization from 1Q jars but every time i do G-G into 1.9Q jars they go bad every time. I have tried using the same hydrated grain that i use in the 1Q jars as well as grain with a lower moisture content (down to 40% vs my normal 52%) have varied the amount of inoculum used (from a couple tablespoons to a half Q) and tried incubating normally as well as on it's side and at an angle yet every time bacteria win out. I have inoculated 1.9 jars from the same master that i used for more 1Q jars and the same thing small jars fine and large jars go bad. Not sure what is going on and would love to hear people thoughts
My normal method with other types of mushrooms is to inoculate quart jars from agar and then use those to inoculate 1.9 Q jars which are then used to  inoculate whatever substrate i am using to fruit from and have succesfully done this with several species of oyster, shittake and cubies.
Any Ideas would be appreciated
Slart


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Pressure cooking shit to kill shit so i can knock it up with my shit to grow shit.
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Invisiblelipa

Registered: 07/24/07
Posts: 2,684
Trusted Cultivator
Re: hericium americanum spawn issues [Re: slartibartfast]
    #21715613 - 05/23/15 08:50 PM (8 years, 8 months ago)

How long are you sterilizing your jars?

You sure your culture is clean? any irregularities in the way the culture is growing?


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Offlineslartibartfast
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Registered: 05/13/08
Posts: 203
Last seen: 3 years, 11 months
Re: hericium americanum spawn issues [Re: lipa]
    #21725205 - 05/26/15 03:05 PM (8 years, 8 months ago)

Culture looks fine to me and in the 1Q jars everything goes great and have used them to spawn to sawdust with good results. With a larger jar i always think moisture content but seeing how as i have tried lowering that and it made no difference i am confused. All in all not a big deal and if my next attempt fails i guess i can just go with the smaller jars but i would like to understand where i have gone wrong. Thanks for the response.
Slart


--------------------
Pressure cooking shit to kill shit so i can knock it up with my shit to grow shit.
Trades welcome


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