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OfflineRabbit


Registered: 02/25/15
Posts: 163
Last seen: 5 years, 8 months
Jars stopped colonization
    #21713311 - 05/23/15 07:36 AM (8 years, 8 months ago)

Ok i made my PF tek jars 2 and a half  months ago and the first month they colonized really fast and now they stopped at 40/50/80% colonization and they havent colonized at all for the 1 and a half month i opened 1 jar and it smells sour should i keep them or trow them away ?


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Invisibleinsanemike

Registered: 02/23/14
Posts: 4,272
Re: Jars stopped colonization [Re: Rabbit]
    #21713347 - 05/23/15 07:58 AM (8 years, 8 months ago)

If they smell sour, toss them.


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OfflineRabbit


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Re: Jars stopped colonization [Re: insanemike]
    #21713467 - 05/23/15 08:47 AM (8 years, 8 months ago)

Can i cut out the colonized mycelium and fruit it ?


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OfflineMajickMuffin
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Re: Jars stopped colonization [Re: Rabbit]
    #21713490 - 05/23/15 08:56 AM (8 years, 8 months ago)

Not if it smells sour...


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Invisibleinsanemike

Registered: 02/23/14
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Re: Jars stopped colonization [Re: MajickMuffin]
    #21713564 - 05/23/15 09:31 AM (8 years, 8 months ago)

It's better to forget your mistake and move on then it is to keep dissapointing yourself. Can you take us through your process so we can help you diagnose your problem and help you find a solution?


Edited by insanemike (05/23/15 09:34 AM)


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Invisiblewowimflabbergasted
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Re: Jars stopped colonization [Re: insanemike]
    #21713606 - 05/23/15 09:48 AM (8 years, 8 months ago)

Quote:

insanemike said:
If they smell sour, toss them.



Especially if they haven't grown in over a month.


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OfflineRabbit


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Re: Jars stopped colonization [Re: insanemike]
    #21713719 - 05/23/15 10:32 AM (8 years, 8 months ago)

I made LC then i made PF substrate verm water flour 2:1:1 then i filled aluminium trays and some jars with it made 6 holes on the trays and 4 on the jar lids then i put alm foil over them PCed them then i inoculated LC in the holes then i incubated them at 26 C and after 1 month 3 of the trays were 100% colonized and the jars were like they are now the rest of the trays werent they were done only the half top and they were too wet on the bottom after 1 more month the trays were contaminated cuz i had opened them... only 1 more was 100% colonized not contaminated and 2 more weeks and the jars still arent moving ...


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OfflineRabbit


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Re: Jars stopped colonization [Re: insanemike]
    #21713727 - 05/23/15 10:35 AM (8 years, 8 months ago)

oh and the mycelium also made holes on the aluminium maybe no air exchange or too much water in the substrate ?


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Invisiblewowimflabbergasted
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Re: Jars stopped colonization [Re: Rabbit]
    #21713736 - 05/23/15 10:37 AM (8 years, 8 months ago)

How did you knock up the LC?


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OfflineGreenRabbit
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Re: Jars stopped colonization [Re: Rabbit]
    #21713932 - 05/23/15 11:56 AM (8 years, 8 months ago)

Quote:

Rabbit said:
Can i cut out the colonized mycelium and fruit it ?





I've done this successfully (using the term lightly) but the jars were at least 50% colonized.

Had a bunch of jars stall out before colonizing the bottoms.


Washed away all the uncolonized parts and spawned to coir. I was expecting it to contam but it didn't.


Ended up with a half oz from what should have been thrown away so I was still pretty happy.


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Offlinespacechildo
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Re: Jars stopped colonization [Re: Rabbit]
    #21713950 - 05/23/15 12:05 PM (8 years, 8 months ago)

Quote:

Rabbit said:
I made LC then i made PF substrate verm water flour 2:1:1 then i filled aluminium trays and some jars with it made 6 holes on the trays and 4 on the jar lids then i put alm foil over them PCed them then i inoculated LC in the holes then i incubated them at 26 C and after 1 month 3 of the trays were 100% colonized and the jars were like they are now the rest of the trays werent they were done only the half top and they were too wet on the bottom after 1 more month the trays were contaminated cuz i had opened them... only 1 more was 100% colonized not contaminated and 2 more weeks and the jars still arent moving ...




yeah that's pretty far out, just follow www.mushroomvideos.com :super:


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Invisibleinsanemike

Registered: 02/23/14
Posts: 4,272
Re: Jars stopped colonization [Re: GreenRabbit]
    #21713960 - 05/23/15 12:07 PM (8 years, 8 months ago)

What is your sterilization procedure? We are looking for the culprit, it's either your cooking process or your inoculation process. If it were mold it would most definitely be your inoculation procedure. Since its bacteria, it could be either.


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OfflineGreenRabbit
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Re: Jars stopped colonization [Re: insanemike]
    #21713980 - 05/23/15 12:13 PM (8 years, 8 months ago)

Quote:

insanemike said:
What is your sterilization procedure? We are looking for the culprit, it's either your cooking process or your inoculation process. If it were mold it would most definitely be your inoculation procedure. Since its bacteria, it could be either.




Been using a SAB lately. I think those were made before I had one though.
Also, I don't have a PC so I kicked up cooking times to 2 hours.

Both have seemed to help.


Edited by GreenRabbit (05/23/15 12:15 PM)


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Invisibleinsanemike

Registered: 02/23/14
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Re: Jars stopped colonization [Re: GreenRabbit]
    #21713993 - 05/23/15 12:18 PM (8 years, 8 months ago)

Lc made from a vendor syringe?


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OfflineGreenRabbit
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Re: Jars stopped colonization [Re: insanemike]
    #21714032 - 05/23/15 12:27 PM (8 years, 8 months ago)

Quote:

insanemike said:
Lc made from a vendor syringe?




I am not OP. I do not use LC I inoculate from spore.


Edited by GreenRabbit (05/23/15 12:28 PM)


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Invisibleinsanemike

Registered: 02/23/14
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Re: Jars stopped colonization [Re: GreenRabbit]
    #21714042 - 05/23/15 12:31 PM (8 years, 8 months ago)

So why are you answering my question to the OP? Create another thread and don't hijack this one, I'm trying to help someone with their grow.


Edited by insanemike (05/23/15 12:34 PM)


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OfflineGreenRabbit
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Re: Jars stopped colonization [Re: insanemike]
    #21714053 - 05/23/15 12:35 PM (8 years, 8 months ago)

Your reply was to me. I was a little confused


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Invisibleinsanemike

Registered: 02/23/14
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Re: Jars stopped colonization [Re: GreenRabbit]
    #21714063 - 05/23/15 12:40 PM (8 years, 8 months ago)

That's because there is other conversation going on that doesn't relate to the OP's thread. The confusion is of your own doing.


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OfflineGreenRabbit
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Re: Jars stopped colonization [Re: insanemike]
    #21714079 - 05/23/15 12:46 PM (8 years, 8 months ago)

lol dude. I quoted OP and made my post, then you posted a reply to me even though I wasn't the most recent post. And OP's name was Rabbit so I thought you thought it was the same person. Fuck it though, I'm out.


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OfflineRabbit


Registered: 02/25/15
Posts: 163
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Re: Jars stopped colonization [Re: insanemike]
    #21714858 - 05/23/15 04:53 PM (8 years, 8 months ago)

Ok ill tell all the details tell me if i did something wrong
I had shrooms from my previous grow so i made spore print from a cap on sterilized foil then i made a syringe using distilled water then i made a LC jar using 4% honey then i inoculated it with the syringe after 1 month the LC was rdy to go i did all above in glove box.I baked the foil in the oven for 2h for the print and the LC i made it using sealed jar with only 1 hole in the cap filled with RTV silicone after i inoculated it i used syringe needle with alcohol cotton on top of it to let clean air in it then i removed the syringe.
After 1 month i made the substrate i bough grains and milled them to flour then i put them in the jars using PF ratio and PCed em for 40mins-1h then i inoculated them in the glove box and left them in the incubator which i sanitize using alcohol and bleach i also use alcohol and bleach in my glove box.


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OfflineRabbit


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Re: Jars stopped colonization [Re: GreenRabbit]
    #21714864 - 05/23/15 04:55 PM (8 years, 8 months ago)

can i use vermiculite instead of coir and if so in what ratio ?


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Invisiblewowimflabbergasted
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Re: Jars stopped colonization [Re: Rabbit]
    #21714915 - 05/23/15 05:09 PM (8 years, 8 months ago)

Print > Syringe > LC is not reliable, especially if you are relatively inexperienced. You need at least 1 hole for GE, and with brf cakes you want 4 holes, without rtv, so you can have 4 inoculation points as well as GE. A sealed jar will stall.


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OfflineRabbit


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Re: Jars stopped colonization [Re: wowimflabbergasted]
    #21714934 - 05/23/15 05:14 PM (8 years, 8 months ago)

i used RTV only for the LC and  think my LC is clean because i made 4 Trays 100% colonized without contams


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OfflineRabbit


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Re: Jars stopped colonization [Re: spacechildo]
    #21714946 - 05/23/15 05:18 PM (8 years, 8 months ago)

Ty for the video i think i found what screwed them up i filled my PC with a lot of water and in the video it says that i shouldn't do that also when i filled them with substrate i also packed it tight and in the video it says that i shouldn't do that too i also forgot to wipe the sides of the jar xD


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OfflineRabbit


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Re: Jars stopped colonization [Re: Rabbit]
    #21714953 - 05/23/15 05:21 PM (8 years, 8 months ago)

Also the ones that got contaminated were the heavy ones they got lot of water in them so they probably got screwed water ratio from the overfilled PC also i haven't gotten green mold yet only this sour smelly bacteria thing.


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Offlinespacechildo
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Re: Jars stopped colonization [Re: Rabbit]
    #21714988 - 05/23/15 05:40 PM (8 years, 8 months ago)

so you put the mix in jars with filtered lids or in trays with poked holes? you're all over the place dude... pc for 40 mins is a fail too..


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Invisiblewowimflabbergasted
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Re: Jars stopped colonization [Re: spacechildo]
    #21715058 - 05/23/15 06:04 PM (8 years, 8 months ago)

Nice avatar space lol


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OfflineRabbit


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Re: Jars stopped colonization [Re: spacechildo]
    #21715103 - 05/23/15 06:14 PM (8 years, 8 months ago)

NO i made holes on the jar lids and i covered them with foil and i covered the trays with 1 layer of foil then i made holes on it and then covered it with another. For how long should i PC em ?


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Invisibleinsanemike

Registered: 02/23/14
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Re: Jars stopped colonization [Re: Rabbit]
    #21715230 - 05/23/15 07:02 PM (8 years, 8 months ago)

Did you flame sterilize your needle before every injection? Next time just to be on the safe side, drop some of your lc on agar first to make sure the culture is clean.


Edited by insanemike (05/23/15 07:03 PM)


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InvisibleSoupSandwich
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Re: Jars stopped colonization [Re: insanemike]
    #21715572 - 05/23/15 08:38 PM (8 years, 8 months ago)

Did you crack the lids?

Sometimes the substrate gets packed down and can't breath.

That's been my experience at least. Before you toss 'em I'd say crack the lid, sniff, and see what happens.


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OfflineRabbit


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Re: Jars stopped colonization [Re: insanemike]
    #21716348 - 05/24/15 02:36 AM (8 years, 8 months ago)

I cant flame sterilise the needles because my glovebox is made from carton but i used new needle for each jar/tray and i wiped them with alcohol.I've never worked with agar how do i check the LC on Agar ?


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InvisibleMad Season
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Re: Jars stopped colonization [Re: Rabbit]
    #21716361 - 05/24/15 02:46 AM (8 years, 8 months ago)

I'd suggest taking your glove box and taking out the gloves, sabs are so much better. Even if you had a flame in the box its purpose would be defeated with a flame moving air around. With a still air box you flame outside the box and work inside the box due to the ease of putting your arms in.

You must flame sterilize the needles. Alcohol won't work. It sanitizes


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Edited by Mad Season (05/24/15 02:49 AM)


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OfflineRabbit


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Re: Jars stopped colonization [Re: Mad Season]
    #21716498 - 05/24/15 04:20 AM (8 years, 8 months ago)

So ill have to make a SAB i guess xD
So can i cut out the colonized parts from the cakes and mix them with vermiculite/soil and at what ratio  ? i dont have access to coir here.


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Invisibleinsanemike

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Re: Jars stopped colonization [Re: Rabbit]
    #21716593 - 05/24/15 05:53 AM (8 years, 8 months ago)

You can do as you stated but you said the cakes smell sour which means they have a bacterial infection. This will most likely yield you little to no mushrooms but on the other hand, you could get lucky. But if I were you, I would toss them cakes and start over.


Edited by insanemike (05/24/15 05:54 AM)


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OfflineRabbit


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Re: Jars stopped colonization [Re: insanemike]
    #21716769 - 05/24/15 07:51 AM (8 years, 8 months ago)

Ok i managed to get 425g of healthy mycelium i removed all the infected areas and washed it well and put it in a 1:100 bleach solution


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OfflineRabbit


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Re: Jars stopped colonization [Re: Rabbit]
    #21717320 - 05/24/15 12:17 PM (8 years, 8 months ago)

Now i baked some soil in the oven but i dont know how much humid should it be


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OfflineGreenRabbit
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Re: Jars stopped colonization [Re: Rabbit] * 1
    #21718401 - 05/24/15 06:11 PM (8 years, 8 months ago)

Quote:

Rabbit said:
Ok i managed to get 425g of healthy mycelium i removed all the infected areas and washed it well and put it in a 1:100 bleach solution




The fuck? Why would you bleach mycelium? And baking soil in the oven? You mean potting soil or what?

Don't bleach mycelium, and next time use bucket tech for bulk


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Invisibleinsanemike

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Re: Jars stopped colonization [Re: GreenRabbit]
    #21719901 - 05/25/15 07:08 AM (8 years, 8 months ago)

This is why he should just toss them cakes and start over. OP needs more experience and more reading before he is ready to do bulk.


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OfflineRabbit


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Re: Jars stopped colonization [Re: insanemike]
    #21721378 - 05/25/15 03:27 PM (8 years, 8 months ago)

I wanted to make sure i killed all the bacteria thats why i bleached i hold it only for 15 minutes then i dunked it in water for 6 hours its not normal soil but i dont know how its called its mix of forest soil dead plants and different animal manture i baked it in the oven for 2-3 hours so that its sterile then after it cooled i crumbled the mycelium and mixed it well


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OfflineRabbit


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Re: Jars stopped colonization [Re: GreenRabbit]
    #21721380 - 05/25/15 03:27 PM (8 years, 8 months ago)

What is this bucket tech ?


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OfflineGreenRabbit
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Re: Jars stopped colonization [Re: Rabbit]
    #21723212 - 05/26/15 12:07 AM (8 years, 8 months ago)

http://www.shroomery.org/forums/showflat.php/Number/18880716#18880716

Basically I just cut as much coir as I think I need for 1 sub off the brick and hydrate with boiling water. Add the verm and stir it all up. Let it cool and you're good to mix with spawn.


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InvisibleLocN9ne
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Re: Jars stopped colonization *DELETED* [Re: GreenRabbit]
    #21723217 - 05/26/15 12:08 AM (8 years, 8 months ago)

Post deleted by LocN9ne

Reason for deletion: I'm dumb



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OfflineRabbit


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Re: Jars stopped colonization *DELETED* [Re: LocN9ne]
    #21751496 - 06/02/15 05:28 AM (8 years, 7 months ago)

So today i checked the tray in which i mixed them mycelium and the soil and the top is like 90% colonized but on the bottom there is little to no colonization should i touched it and i think it has lost some of its moisture should i add some water or not ? I've sealed the tray and put 1 big patch of tyvek on it when should i remove the seal ? Does it have to be 100% colonized when it is mixed with soil ?


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OfflineRabbit


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Re: Jars stopped colonization *DELETED* [Re: Rabbit]
    #21751502 - 06/02/15 05:32 AM (8 years, 7 months ago)

BTW today i made the best spore print i have ever made it has TONS Of Spores its sooo black and dense and its big 8cm diameter what will be the most useful way to use it ?


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Invisibleinsanemike

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Re: Jars stopped colonization *DELETED* [Re: Rabbit]
    #21751578 - 06/02/15 06:39 AM (8 years, 7 months ago)

Put some on agar and save the rest.


Edited by insanemike (06/02/15 06:40 AM)


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