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OfflineRabbit


Registered: 02/25/15
Posts: 163
Last seen: 5 years, 8 months
Re: Jars stopped colonization [Re: GreenRabbit]
    #21714864 - 05/23/15 04:55 PM (8 years, 8 months ago)

can i use vermiculite instead of coir and if so in what ratio ?


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Invisiblewowimflabbergasted
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Registered: 07/16/12
Posts: 18,918
Re: Jars stopped colonization [Re: Rabbit]
    #21714915 - 05/23/15 05:09 PM (8 years, 8 months ago)

Print > Syringe > LC is not reliable, especially if you are relatively inexperienced. You need at least 1 hole for GE, and with brf cakes you want 4 holes, without rtv, so you can have 4 inoculation points as well as GE. A sealed jar will stall.


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OfflineRabbit


Registered: 02/25/15
Posts: 163
Last seen: 5 years, 8 months
Re: Jars stopped colonization [Re: wowimflabbergasted]
    #21714934 - 05/23/15 05:14 PM (8 years, 8 months ago)

i used RTV only for the LC and  think my LC is clean because i made 4 Trays 100% colonized without contams


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OfflineRabbit


Registered: 02/25/15
Posts: 163
Last seen: 5 years, 8 months
Re: Jars stopped colonization [Re: spacechildo]
    #21714946 - 05/23/15 05:18 PM (8 years, 8 months ago)

Ty for the video i think i found what screwed them up i filled my PC with a lot of water and in the video it says that i shouldn't do that also when i filled them with substrate i also packed it tight and in the video it says that i shouldn't do that too i also forgot to wipe the sides of the jar xD


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OfflineRabbit


Registered: 02/25/15
Posts: 163
Last seen: 5 years, 8 months
Re: Jars stopped colonization [Re: Rabbit]
    #21714953 - 05/23/15 05:21 PM (8 years, 8 months ago)

Also the ones that got contaminated were the heavy ones they got lot of water in them so they probably got screwed water ratio from the overfilled PC also i haven't gotten green mold yet only this sour smelly bacteria thing.


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Offlinespacechildo
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Registered: 01/24/13
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Re: Jars stopped colonization [Re: Rabbit]
    #21714988 - 05/23/15 05:40 PM (8 years, 8 months ago)

so you put the mix in jars with filtered lids or in trays with poked holes? you're all over the place dude... pc for 40 mins is a fail too..


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Invisiblewowimflabbergasted
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Registered: 07/16/12
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Re: Jars stopped colonization [Re: spacechildo]
    #21715058 - 05/23/15 06:04 PM (8 years, 8 months ago)

Nice avatar space lol


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OfflineRabbit


Registered: 02/25/15
Posts: 163
Last seen: 5 years, 8 months
Re: Jars stopped colonization [Re: spacechildo]
    #21715103 - 05/23/15 06:14 PM (8 years, 8 months ago)

NO i made holes on the jar lids and i covered them with foil and i covered the trays with 1 layer of foil then i made holes on it and then covered it with another. For how long should i PC em ?


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Invisibleinsanemike

Registered: 02/23/14
Posts: 4,272
Re: Jars stopped colonization [Re: Rabbit]
    #21715230 - 05/23/15 07:02 PM (8 years, 8 months ago)

Did you flame sterilize your needle before every injection? Next time just to be on the safe side, drop some of your lc on agar first to make sure the culture is clean.


Edited by insanemike (05/23/15 07:03 PM)


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InvisibleSoupSandwich
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Registered: 12/08/14
Posts: 4,440
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Re: Jars stopped colonization [Re: insanemike]
    #21715572 - 05/23/15 08:38 PM (8 years, 8 months ago)

Did you crack the lids?

Sometimes the substrate gets packed down and can't breath.

That's been my experience at least. Before you toss 'em I'd say crack the lid, sniff, and see what happens.


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OfflineRabbit


Registered: 02/25/15
Posts: 163
Last seen: 5 years, 8 months
Re: Jars stopped colonization [Re: insanemike]
    #21716348 - 05/24/15 02:36 AM (8 years, 8 months ago)

I cant flame sterilise the needles because my glovebox is made from carton but i used new needle for each jar/tray and i wiped them with alcohol.I've never worked with agar how do i check the LC on Agar ?


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InvisibleMad Season
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Re: Jars stopped colonization [Re: Rabbit]
    #21716361 - 05/24/15 02:46 AM (8 years, 8 months ago)

I'd suggest taking your glove box and taking out the gloves, sabs are so much better. Even if you had a flame in the box its purpose would be defeated with a flame moving air around. With a still air box you flame outside the box and work inside the box due to the ease of putting your arms in.

You must flame sterilize the needles. Alcohol won't work. It sanitizes


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Edited by Mad Season (05/24/15 02:49 AM)


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OfflineRabbit


Registered: 02/25/15
Posts: 163
Last seen: 5 years, 8 months
Re: Jars stopped colonization [Re: Mad Season]
    #21716498 - 05/24/15 04:20 AM (8 years, 8 months ago)

So ill have to make a SAB i guess xD
So can i cut out the colonized parts from the cakes and mix them with vermiculite/soil and at what ratio  ? i dont have access to coir here.


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Invisibleinsanemike

Registered: 02/23/14
Posts: 4,272
Re: Jars stopped colonization [Re: Rabbit]
    #21716593 - 05/24/15 05:53 AM (8 years, 8 months ago)

You can do as you stated but you said the cakes smell sour which means they have a bacterial infection. This will most likely yield you little to no mushrooms but on the other hand, you could get lucky. But if I were you, I would toss them cakes and start over.


Edited by insanemike (05/24/15 05:54 AM)


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OfflineRabbit


Registered: 02/25/15
Posts: 163
Last seen: 5 years, 8 months
Re: Jars stopped colonization [Re: insanemike]
    #21716769 - 05/24/15 07:51 AM (8 years, 8 months ago)

Ok i managed to get 425g of healthy mycelium i removed all the infected areas and washed it well and put it in a 1:100 bleach solution


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OfflineRabbit


Registered: 02/25/15
Posts: 163
Last seen: 5 years, 8 months
Re: Jars stopped colonization [Re: Rabbit]
    #21717320 - 05/24/15 12:17 PM (8 years, 8 months ago)

Now i baked some soil in the oven but i dont know how much humid should it be


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OfflineGreenRabbit
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Registered: 04/28/13
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Re: Jars stopped colonization [Re: Rabbit] * 1
    #21718401 - 05/24/15 06:11 PM (8 years, 8 months ago)

Quote:

Rabbit said:
Ok i managed to get 425g of healthy mycelium i removed all the infected areas and washed it well and put it in a 1:100 bleach solution




The fuck? Why would you bleach mycelium? And baking soil in the oven? You mean potting soil or what?

Don't bleach mycelium, and next time use bucket tech for bulk


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Invisibleinsanemike

Registered: 02/23/14
Posts: 4,272
Re: Jars stopped colonization [Re: GreenRabbit]
    #21719901 - 05/25/15 07:08 AM (8 years, 8 months ago)

This is why he should just toss them cakes and start over. OP needs more experience and more reading before he is ready to do bulk.


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OfflineRabbit


Registered: 02/25/15
Posts: 163
Last seen: 5 years, 8 months
Re: Jars stopped colonization [Re: insanemike]
    #21721378 - 05/25/15 03:27 PM (8 years, 8 months ago)

I wanted to make sure i killed all the bacteria thats why i bleached i hold it only for 15 minutes then i dunked it in water for 6 hours its not normal soil but i dont know how its called its mix of forest soil dead plants and different animal manture i baked it in the oven for 2-3 hours so that its sterile then after it cooled i crumbled the mycelium and mixed it well


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OfflineRabbit


Registered: 02/25/15
Posts: 163
Last seen: 5 years, 8 months
Re: Jars stopped colonization [Re: GreenRabbit]
    #21721380 - 05/25/15 03:27 PM (8 years, 8 months ago)

What is this bucket tech ?


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