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OfflineRabbit


Registered: 02/25/15
Posts: 163
Last seen: 5 years, 8 months
Jars stopped colonization
    #21713311 - 05/23/15 07:36 AM (8 years, 8 months ago)

Ok i made my PF tek jars 2 and a half  months ago and the first month they colonized really fast and now they stopped at 40/50/80% colonization and they havent colonized at all for the 1 and a half month i opened 1 jar and it smells sour should i keep them or trow them away ?


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Invisibleinsanemike

Registered: 02/23/14
Posts: 4,272
Re: Jars stopped colonization [Re: Rabbit]
    #21713347 - 05/23/15 07:58 AM (8 years, 8 months ago)

If they smell sour, toss them.


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OfflineRabbit


Registered: 02/25/15
Posts: 163
Last seen: 5 years, 8 months
Re: Jars stopped colonization [Re: insanemike]
    #21713467 - 05/23/15 08:47 AM (8 years, 8 months ago)

Can i cut out the colonized mycelium and fruit it ?


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OfflineMajickMuffin
Edible Cult


Registered: 05/28/14
Posts: 4,345
Loc: North
Last seen: 2 years, 8 months
Re: Jars stopped colonization [Re: Rabbit]
    #21713490 - 05/23/15 08:56 AM (8 years, 8 months ago)

Not if it smells sour...


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Invisibleinsanemike

Registered: 02/23/14
Posts: 4,272
Re: Jars stopped colonization [Re: MajickMuffin]
    #21713564 - 05/23/15 09:31 AM (8 years, 8 months ago)

It's better to forget your mistake and move on then it is to keep dissapointing yourself. Can you take us through your process so we can help you diagnose your problem and help you find a solution?


Edited by insanemike (05/23/15 09:34 AM)


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Invisiblewowimflabbergasted
supercalifragilistic
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Registered: 07/16/12
Posts: 18,918
Re: Jars stopped colonization [Re: insanemike]
    #21713606 - 05/23/15 09:48 AM (8 years, 8 months ago)

Quote:

insanemike said:
If they smell sour, toss them.



Especially if they haven't grown in over a month.


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OfflineRabbit


Registered: 02/25/15
Posts: 163
Last seen: 5 years, 8 months
Re: Jars stopped colonization [Re: insanemike]
    #21713719 - 05/23/15 10:32 AM (8 years, 8 months ago)

I made LC then i made PF substrate verm water flour 2:1:1 then i filled aluminium trays and some jars with it made 6 holes on the trays and 4 on the jar lids then i put alm foil over them PCed them then i inoculated LC in the holes then i incubated them at 26 C and after 1 month 3 of the trays were 100% colonized and the jars were like they are now the rest of the trays werent they were done only the half top and they were too wet on the bottom after 1 more month the trays were contaminated cuz i had opened them... only 1 more was 100% colonized not contaminated and 2 more weeks and the jars still arent moving ...


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OfflineRabbit


Registered: 02/25/15
Posts: 163
Last seen: 5 years, 8 months
Re: Jars stopped colonization [Re: insanemike]
    #21713727 - 05/23/15 10:35 AM (8 years, 8 months ago)

oh and the mycelium also made holes on the aluminium maybe no air exchange or too much water in the substrate ?


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Invisiblewowimflabbergasted
supercalifragilistic
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Registered: 07/16/12
Posts: 18,918
Re: Jars stopped colonization [Re: Rabbit]
    #21713736 - 05/23/15 10:37 AM (8 years, 8 months ago)

How did you knock up the LC?


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OfflineGreenRabbit
Plutonium Pollinator
Male


Registered: 04/28/13
Posts: 2,667
Loc: In a forest
Last seen: 2 years, 3 months
Re: Jars stopped colonization [Re: Rabbit]
    #21713932 - 05/23/15 11:56 AM (8 years, 8 months ago)

Quote:

Rabbit said:
Can i cut out the colonized mycelium and fruit it ?





I've done this successfully (using the term lightly) but the jars were at least 50% colonized.

Had a bunch of jars stall out before colonizing the bottoms.


Washed away all the uncolonized parts and spawned to coir. I was expecting it to contam but it didn't.


Ended up with a half oz from what should have been thrown away so I was still pretty happy.


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Offlinespacechildo
proletarians rise up
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Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
Re: Jars stopped colonization [Re: Rabbit]
    #21713950 - 05/23/15 12:05 PM (8 years, 8 months ago)

Quote:

Rabbit said:
I made LC then i made PF substrate verm water flour 2:1:1 then i filled aluminium trays and some jars with it made 6 holes on the trays and 4 on the jar lids then i put alm foil over them PCed them then i inoculated LC in the holes then i incubated them at 26 C and after 1 month 3 of the trays were 100% colonized and the jars were like they are now the rest of the trays werent they were done only the half top and they were too wet on the bottom after 1 more month the trays were contaminated cuz i had opened them... only 1 more was 100% colonized not contaminated and 2 more weeks and the jars still arent moving ...




yeah that's pretty far out, just follow www.mushroomvideos.com :super:


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Invisibleinsanemike

Registered: 02/23/14
Posts: 4,272
Re: Jars stopped colonization [Re: GreenRabbit]
    #21713960 - 05/23/15 12:07 PM (8 years, 8 months ago)

What is your sterilization procedure? We are looking for the culprit, it's either your cooking process or your inoculation process. If it were mold it would most definitely be your inoculation procedure. Since its bacteria, it could be either.


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OfflineGreenRabbit
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Male


Registered: 04/28/13
Posts: 2,667
Loc: In a forest
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Re: Jars stopped colonization [Re: insanemike]
    #21713980 - 05/23/15 12:13 PM (8 years, 8 months ago)

Quote:

insanemike said:
What is your sterilization procedure? We are looking for the culprit, it's either your cooking process or your inoculation process. If it were mold it would most definitely be your inoculation procedure. Since its bacteria, it could be either.




Been using a SAB lately. I think those were made before I had one though.
Also, I don't have a PC so I kicked up cooking times to 2 hours.

Both have seemed to help.


Edited by GreenRabbit (05/23/15 12:15 PM)


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Invisibleinsanemike

Registered: 02/23/14
Posts: 4,272
Re: Jars stopped colonization [Re: GreenRabbit]
    #21713993 - 05/23/15 12:18 PM (8 years, 8 months ago)

Lc made from a vendor syringe?


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OfflineGreenRabbit
Plutonium Pollinator
Male


Registered: 04/28/13
Posts: 2,667
Loc: In a forest
Last seen: 2 years, 3 months
Re: Jars stopped colonization [Re: insanemike]
    #21714032 - 05/23/15 12:27 PM (8 years, 8 months ago)

Quote:

insanemike said:
Lc made from a vendor syringe?




I am not OP. I do not use LC I inoculate from spore.


Edited by GreenRabbit (05/23/15 12:28 PM)


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Invisibleinsanemike

Registered: 02/23/14
Posts: 4,272
Re: Jars stopped colonization [Re: GreenRabbit]
    #21714042 - 05/23/15 12:31 PM (8 years, 8 months ago)

So why are you answering my question to the OP? Create another thread and don't hijack this one, I'm trying to help someone with their grow.


Edited by insanemike (05/23/15 12:34 PM)


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OfflineGreenRabbit
Plutonium Pollinator
Male


Registered: 04/28/13
Posts: 2,667
Loc: In a forest
Last seen: 2 years, 3 months
Re: Jars stopped colonization [Re: insanemike]
    #21714053 - 05/23/15 12:35 PM (8 years, 8 months ago)

Your reply was to me. I was a little confused


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Invisibleinsanemike

Registered: 02/23/14
Posts: 4,272
Re: Jars stopped colonization [Re: GreenRabbit]
    #21714063 - 05/23/15 12:40 PM (8 years, 8 months ago)

That's because there is other conversation going on that doesn't relate to the OP's thread. The confusion is of your own doing.


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OfflineGreenRabbit
Plutonium Pollinator
Male


Registered: 04/28/13
Posts: 2,667
Loc: In a forest
Last seen: 2 years, 3 months
Re: Jars stopped colonization [Re: insanemike]
    #21714079 - 05/23/15 12:46 PM (8 years, 8 months ago)

lol dude. I quoted OP and made my post, then you posted a reply to me even though I wasn't the most recent post. And OP's name was Rabbit so I thought you thought it was the same person. Fuck it though, I'm out.


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OfflineRabbit


Registered: 02/25/15
Posts: 163
Last seen: 5 years, 8 months
Re: Jars stopped colonization [Re: insanemike]
    #21714858 - 05/23/15 04:53 PM (8 years, 8 months ago)

Ok ill tell all the details tell me if i did something wrong
I had shrooms from my previous grow so i made spore print from a cap on sterilized foil then i made a syringe using distilled water then i made a LC jar using 4% honey then i inoculated it with the syringe after 1 month the LC was rdy to go i did all above in glove box.I baked the foil in the oven for 2h for the print and the LC i made it using sealed jar with only 1 hole in the cap filled with RTV silicone after i inoculated it i used syringe needle with alcohol cotton on top of it to let clean air in it then i removed the syringe.
After 1 month i made the substrate i bough grains and milled them to flour then i put them in the jars using PF ratio and PCed em for 40mins-1h then i inoculated them in the glove box and left them in the incubator which i sanitize using alcohol and bleach i also use alcohol and bleach in my glove box.


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