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OfflineZephen
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Registered: 04/16/15
Posts: 324
Loc: Land of the Phoenix
Last seen: 4 months, 24 days
Spore syringe question.
    #21709107 - 05/22/15 04:03 AM (8 years, 8 months ago)

I brought home some sterile saline flushes from work. Could I use the saline to make a spore syringe with or does it have to be boiled distilled water?


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OfflineShroominMe
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Registered: 05/03/15
Posts: 525
Last seen: 8 years, 3 months
Re: Spore syringe question. [Re: Zephen]
    #21709122 - 05/22/15 04:16 AM (8 years, 8 months ago)

The salt will inhibit myc production http://www.shroomery.org/forums/showflat.php/Number/1877689

I'd go ahead and break out the syringes, pump out the saline water, fill the syringe completely with DH20 and eject the water out into your sink.  Repeat several times and then pull up enough DH20 to half fill your syringe and PC for 1/2 hr at 15 psi.

Others have success with using water boiled for 10 minutes to 'sterilize' their syringes but I personally don't recommend it.


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OfflineZephen
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Registered: 04/16/15
Posts: 324
Loc: Land of the Phoenix
Last seen: 4 months, 24 days
Re: Spore syringe question. [Re: ShroominMe]
    #21709126 - 05/22/15 04:19 AM (8 years, 8 months ago)

Quote:

ShroominMe said:
The salt will inhibit myc production http://www.shroomery.org/forums/showflat.php/Number/1877689

I'd go ahead and break out the syringes, pump out the saline water, fill the syringe completely with DH20 and eject the water out into your sink.  Repeat several times and then pull up enough DH20 to half fill your syringe and PC for 1/2 hr at 15 psi.

Others have success with using water boiled for 10 minutes to 'sterilize' their syringes but I personally don't recommend it.




Thanks bro! Answers my question :seriousthumbsup:


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InvisiblebodhisattaMDiscordReddit
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Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Spore syringe question. [Re: Zephen]
    #21709162 - 05/22/15 05:01 AM (8 years, 8 months ago)

Boil water on your stove suck it up squirt it out 2-3 times. If you PC a syringe with any water in it most will come out by the end of the cycle. If you PC a syringe 10minutes is more than enough. 30 hour is super overkill agar only needs 15


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