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Offlinem0rph3us22
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Registered: 03/03/15
Posts: 40
Last seen: 4 years, 9 months
Contamination
    #21708743 - 05/22/15 12:26 AM (8 years, 8 months ago)

is contamination really as frequent as described? I mean for my first few tries should i expect contamination? And should i be overly thorough with sterilizing?


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Invisibleinsanemike

Registered: 02/23/14
Posts: 4,272
Re: Contamination [Re: m0rph3us22]
    #21708765 - 05/22/15 12:35 AM (8 years, 8 months ago)

Contamination occurs even to the best of cultivators from time to time. The more experience you gain, the less frequent you should see them pop up. Or, you could be a maestro and have great sterile technique from the start. Just find a proven tek, folllow it to a T and be patient.


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OfflineLaughingcowwa
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Registered: 05/02/15
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Re: Contamination [Re: insanemike]
    #21708792 - 05/22/15 12:47 AM (8 years, 8 months ago)

Just go heavy with the glen 20 and make a sterile environment you'll be fine. not so hard.


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Invisibleoregon.trailboy
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Registered: 12/15/14
Posts: 1,641
Loc: Oregon
Re: Contamination [Re: insanemike] * 1
    #21708801 - 05/22/15 12:52 AM (8 years, 8 months ago)

Flame = sterilize and chemicals = sanitize. Remember to use flame sterilization as a last step for cleaning needles and metal tools. A still air box is a very important tool. Agar is a mycologist mans best friend when it comes to cleaning up and avoiding contamination! Good luck growing :smile:


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Offlinem0rph3us22
m0rph3us
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Registered: 03/03/15
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Last seen: 4 years, 9 months
Re: Contamination [Re: oregon.trailboy]
    #21708820 - 05/22/15 01:01 AM (8 years, 8 months ago)

Hmm, ok thank you! now is it possible to make spore syringes without a laminar flow hood? like could i just disinfect the area intensely with disinfectant spray before hand, or during the process?


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Invisibleoregon.trailboy
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Registered: 12/15/14
Posts: 1,641
Loc: Oregon
Re: Contamination [Re: m0rph3us22] * 1
    #21708877 - 05/22/15 01:24 AM (8 years, 8 months ago)

Make them in a still air box. Use as sterile equipment as possible and no moving air and flame sterilization and you could have yourself a good spore syringe. Go on you tube an look up let's grow mushrooms. It's a series of videos by a mycology god who makes some great sterile technique!


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Offlinekeeno
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Registered: 06/01/11
Posts: 2,679
Loc: UK Flag
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Re: Contamination [Re: oregon.trailboy]
    #21709049 - 05/22/15 03:09 AM (8 years, 8 months ago)

^ this

it's well worth doing as much cleaning up as you can, closing windows in the room you're gonna use an hour or so before you do any work, have a shower before, brush your teeth, get some hand sanitizer, wear some clean clothes, make a Still Air Box, get a decent flame to heat up your needles (blue flame, not a yellow one)
Sounds a bit extreme, but it's a right bastard to see 6 weeks worth of work go all mouldy and fuck up in front of you

good luck!
:asianofapproval:


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