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Offlinemycologyinfo
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MS grow to strain isolation
    #21707663 - 05/21/15 07:45 PM (8 years, 8 months ago)

I'm planning on doing a monotub grow from a MS syringe. Once I grow out the fruits, how do I isolate a culture? should I just pick the biggest fruit and print it or clone it on agar? after that, will i need to isolate it further? a little help here please. thanks


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InvisibleHashish
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Re: MS grow to strain isolation [Re: mycologyinfo]
    #21707683 - 05/21/15 07:50 PM (8 years, 8 months ago)

pick a small pin from a cluster and clone that on agar. some of the links in my sig may be helpful


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Offlinemushpunx
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Re: MS grow to strain isolation [Re: Hashish]
    #21707712 - 05/21/15 07:57 PM (8 years, 8 months ago)



You would wanna take a pin from a fast growing culture, or any other mushroom with desirable traits to clone.
This will be a clone, you can isolate sectors from it if you like and test them out but it will still be a clone.


If you are after an isolate, the process is essentially to keep making transfers away from your germination plate untill you see defined sectoring, then isolate each sector to a new plate untill they no longer show any sectoring... then test out each isolate and pick the winner.

Both are great ways to get great cultures!


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Offlinemycologyinfo
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Re: MS grow to strain isolation [Re: mushpunx]
    #21710067 - 05/22/15 10:57 AM (8 years, 8 months ago)

Not necessarily looking for an isolate. Just want to "selective breed" a MS grow to achieve a repeatable, abundant grow. so should I clone the biggest fruits and groe out those?
To clarify, how should I achieve genetics to give me the most repeatable, abundant grows?
thanks


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Offlinemycologyinfo
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Re: MS grow to strain isolation [Re: Hashish]
    #21710090 - 05/22/15 11:05 AM (8 years, 8 months ago)

I have a general understandING of HOW to clone. I'm not sure if I'm asking the right question


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InvisibleLocN9ne
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Re: MS grow to strain isolation [Re: mycologyinfo]
    #21710114 - 05/22/15 11:11 AM (8 years, 8 months ago)

If you clone once there are still gonna be variable characteristics in growth patterns...it's not really an identical clone... he is talking about sectoring the different growing pattern of the mycellium until you have only one style of growth that is agreessive  (or whatever characteristics your searching for)...so pretty much if your gonna clone you might as well continue to isolate... I don't know if that helped...


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OfflineGreenRabbit
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Re: MS grow to strain isolation [Re: mycologyinfo]
    #21710125 - 05/22/15 11:13 AM (8 years, 8 months ago)

Take your favorite shroom and clone that one, or clone a pin from the cluster if there is one.

You can keep taking clones from the next generation to get closer to your desired traits.
Since you are starting from MS, the genetics will not be the same in every generation.


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Offlinemycologyinfo
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Re: MS grow to strain isolation [Re: LocN9ne]
    #21710152 - 05/22/15 11:20 AM (8 years, 8 months ago)

It did, thanks. Just not quite what I'm looking for.


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Offlinemycologyinfo
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Re: MS grow to strain isolation [Re: GreenRabbit]
    #21710156 - 05/22/15 11:21 AM (8 years, 8 months ago)

So if biggest is my favorite, then keep cloning the largest fruit?
I'm just wondering about productivity. Like how to achieve the best flushes. Which I know isn't entirely genetics, but it does play a part.


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OfflineGreenRabbit
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Re: MS grow to strain isolation [Re: mycologyinfo]
    #21710165 - 05/22/15 11:27 AM (8 years, 8 months ago)

Quote:

mycologyinfo said:
So if biggest is my favorite, then keep cloning the largest fruit?
I'm just wondering about productivity. Like how to achieve the best flushes. Which I know isn't entirely genetics, but it does play a part.




Grow for potency not size. Both is great if you can get it haha.

I'd suggest you take many clones, then sample them. You don't know how potent they are until you eat them, and by then there's no shroom to take a clone of lol. So you have to make clones before you know how potent they are.


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Offlinemycologyinfo
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Re: MS grow to strain isolation [Re: GreenRabbit]
    #21710184 - 05/22/15 11:35 AM (8 years, 8 months ago)

Alright. Lol. Is there any genetic way to increase flush size or is that just technique?


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OfflineGreenRabbit
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Re: MS grow to strain isolation [Re: mycologyinfo]
    #21710211 - 05/22/15 11:45 AM (8 years, 8 months ago)

Quote:

mycologyinfo said:
Alright. Lol. Is there any genetic way to increase flush size or is that just technique?




Genetics are probably the most important factor in how a strain grows. Poor grow conditions will of course diminish yields though.


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Offlinemycologyinfo
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Re: MS grow to strain isolation [Re: GreenRabbit]
    #21710260 - 05/22/15 12:02 PM (8 years, 8 months ago)

So is there any way to "breed" out better flushes?


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Offlinemushpunx
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Re: MS grow to strain isolation [Re: mycologyinfo]
    #21710920 - 05/22/15 03:07 PM (8 years, 8 months ago)

Lots of testing and searching for winners.

Im in the process of testing out lots of AA clomes Id taken. See the tubs in my  sig? Those were clones taken from great clusters, I still have a lot of them to test but so far the three tubs that fruited already were pretty awesome. I still have to try them out to test potency.

Essentially Im going to pick the very best out of the best, and slant it.

But yea, at that point you could continue to work on the clone, isolating out each sector, then fruiting and testing out each one again.. but most of the time the clone is fine as is.


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Offlinemycologyinfo
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Re: MS grow to strain isolation [Re: mushpunx]
    #21711478 - 05/22/15 05:34 PM (8 years, 8 months ago)

Alright thanks a bunch. Some nice tubs there too


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InvisibleLocN9ne
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Re: MS grow to strain isolation [Re: mycologyinfo]
    #21711483 - 05/22/15 05:35 PM (8 years, 8 months ago)

Mushpunx...your sig makes me want eggs lol...excellent grows


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Offlinemushpunx
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Re: MS grow to strain isolation [Re: LocN9ne]
    #21711581 - 05/22/15 06:08 PM (8 years, 8 months ago)

Egg? Hahaha I never noticed!

Thanks! You really gotta test each clone out though man you might have an excellent fruiter that isnt all that potent.. if youre unlucky haha


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