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Offlinem0rph3us22
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Spore prints and inoculation
    #21704800 - 05/20/15 11:48 PM (8 years, 8 months ago)

I have ordered some spore prints on foil, how do i use these? do i scrape them into the culture medium or do i some how get it into a syringe and inoculate that way?


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InvisibleMad Season
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Re: Spore prints and inoculation [Re: m0rph3us22]
    #21704820 - 05/20/15 11:58 PM (8 years, 8 months ago)

There's a few ways of doing it but I HIGHLY recommend putting it onto agar. Spore prints are never clean. It's also a lot easier to do.

You can scrape it with an inoculation loop and rub it on the agar or you can take a qtip and pc it then rub it on the print and onto the agar.

If you're still wanting to make a syringe you can use a small sharp knife/xacto to scrape into sterile water. Or use a qtip that was pcd  and swirled in sterile water.


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Invisiblewowimflabbergasted
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Re: Spore prints and inoculation [Re: Mad Season]
    #21704858 - 05/21/15 12:15 AM (8 years, 8 months ago)



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OfflineApps
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Re: Spore prints and inoculation [Re: Mad Season]
    #21704865 - 05/21/15 12:16 AM (8 years, 8 months ago)

Quote:

Mad Season said:
There's a few ways of doing it but I HIGHLY recommend putting it onto agar. Spore prints are never clean. It's also a lot easier to do.

You can scrape it with an inoculation loop and rub it on the agar or you can take a qtip and pc it then rub it on the print and onto the agar.

If you're still wanting to make a syringe you can use a small sharp knife/xacto to scrape into sterile water. Or use a qtip that was pcd  and swirled in sterile water.




I would avoid sharp instruments. You could damage the spores. I personally like to just put spores on agar. Making a syringe you would still want to test it out  so that would just be extra work/risk.


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Offlinem0rph3us22
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Re: Spore prints and inoculation [Re: Apps]
    #21704923 - 05/21/15 12:34 AM (8 years, 8 months ago)

I am a hardcore noob, this is the first cultivation i have ever done! how do i make a concise agar?


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Your Just a Hologram living a Holographic Life, and theres No such thing as anything Your NoT EvEn ALiVe!!!
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Edited by m0rph3us22 (05/21/15 01:05 AM)


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OfflineApps
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Re: Spore prints and inoculation [Re: m0rph3us22]
    #21705006 - 05/21/15 01:20 AM (8 years, 8 months ago)

I didn't start with these,  but I  use this tek now because the supplies are available everywhere and cheap

http://www.shroomery.org/forums/showflat.php/Number/19208976


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InvisibleNumeroEno
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Re: Spore prints and inoculation [Re: Apps]
    #21705022 - 05/21/15 01:29 AM (8 years, 8 months ago)

Either make a syringe and inoculate PF cakes, or start some no-pour agar plates using this tek. Check the link in my sig for a nifty way to inoculate agar plates with the print.

http://www.shroomery.org/forums/showflat.php/Number/19208976/fpart/1/vc/1/nt/134

If you don't own a pressure cooker you can make a spore syringe and do the PF tek without one. All you really need is to construct a SAB.


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Edited by NumeroEno (05/21/15 01:30 AM)


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