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m0rph3us22
m0rph3us

Registered: 03/03/15
Posts: 40
Last seen: 4 years, 9 months
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Spore prints and inoculation
#21704800 - 05/20/15 11:48 PM (8 years, 8 months ago) |
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I have ordered some spore prints on foil, how do i use these? do i scrape them into the culture medium or do i some how get it into a syringe and inoculate that way?
-------------------- Your Just a Hologram living a Holographic Life, and theres No such thing as anything Your NoT EvEn ALiVe!!! Some Say an Alien Race Created us All
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Spore prints and inoculation [Re: m0rph3us22]
#21704820 - 05/20/15 11:58 PM (8 years, 8 months ago) |
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There's a few ways of doing it but I HIGHLY recommend putting it onto agar. Spore prints are never clean. It's also a lot easier to do.
You can scrape it with an inoculation loop and rub it on the agar or you can take a qtip and pc it then rub it on the print and onto the agar.
If you're still wanting to make a syringe you can use a small sharp knife/xacto to scrape into sterile water. Or use a qtip that was pcd and swirled in sterile water.
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wowimflabbergasted
supercalifragilistic



Registered: 07/16/12
Posts: 18,918
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Re: Spore prints and inoculation [Re: Mad Season]
#21704858 - 05/21/15 12:15 AM (8 years, 8 months ago) |
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Apps
Code Junkie



Registered: 09/05/14
Posts: 138
Loc: Southwest USA
Last seen: 10 months, 11 days
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Re: Spore prints and inoculation [Re: Mad Season]
#21704865 - 05/21/15 12:16 AM (8 years, 8 months ago) |
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Quote:
Mad Season said: There's a few ways of doing it but I HIGHLY recommend putting it onto agar. Spore prints are never clean. It's also a lot easier to do.
You can scrape it with an inoculation loop and rub it on the agar or you can take a qtip and pc it then rub it on the print and onto the agar.
If you're still wanting to make a syringe you can use a small sharp knife/xacto to scrape into sterile water. Or use a qtip that was pcd and swirled in sterile water.
I would avoid sharp instruments. You could damage the spores. I personally like to just put spores on agar. Making a syringe you would still want to test it out so that would just be extra work/risk.
-------------------- To be lazy is to die.
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m0rph3us22
m0rph3us

Registered: 03/03/15
Posts: 40
Last seen: 4 years, 9 months
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Re: Spore prints and inoculation [Re: Apps]
#21704923 - 05/21/15 12:34 AM (8 years, 8 months ago) |
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I am a hardcore noob, this is the first cultivation i have ever done! how do i make a concise agar?
-------------------- Your Just a Hologram living a Holographic Life, and theres No such thing as anything Your NoT EvEn ALiVe!!! Some Say an Alien Race Created us All
Edited by m0rph3us22 (05/21/15 01:05 AM)
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Apps
Code Junkie



Registered: 09/05/14
Posts: 138
Loc: Southwest USA
Last seen: 10 months, 11 days
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Re: Spore prints and inoculation [Re: m0rph3us22]
#21705006 - 05/21/15 01:20 AM (8 years, 8 months ago) |
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I didn't start with these, but I use this tek now because the supplies are available everywhere and cheap
http://www.shroomery.org/forums/showflat.php/Number/19208976
-------------------- To be lazy is to die.
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NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
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Re: Spore prints and inoculation [Re: Apps]
#21705022 - 05/21/15 01:29 AM (8 years, 8 months ago) |
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Either make a syringe and inoculate PF cakes, or start some no-pour agar plates using this tek. Check the link in my sig for a nifty way to inoculate agar plates with the print.
http://www.shroomery.org/forums/showflat.php/Number/19208976/fpart/1/vc/1/nt/134
If you don't own a pressure cooker you can make a spore syringe and do the PF tek without one. All you really need is to construct a SAB.
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Let it grow! Let it grow! Greatly yield! What shall we say, shall we call it by a name As well to count the angels dancing on a pin Water bright as the sky from which it came And the name is on the earth that takes it in DOG FOOD AGAR MY ELECTRIC INOCULATION LOOP
Edited by NumeroEno (05/21/15 01:30 AM)
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