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OfflineDeadPhan
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had to do g2g transfers today with 50% isopropyl wintergreen flavor...
    #21703285 - 05/20/15 05:41 PM (8 years, 8 months ago)

Only thing i could find and was pressed for time. I looked up threads about 50% but there were none. Only some stating anywhere between 60 and 70 were good. Definitly noticed a difference in its strength just when rubbing on my hands. Usualy ill get a kind of tingle when i use stronger. Also like little stings from tiny cuts on hands. This nothing. Anyway.....i did them anyway. I was in front of flowhood so chances arent too nill. We shall see though. The worst part was the wintergreen though. I love to smell the master jar after transfers and the lid right before. To make sure it didnt smell too funky and just because i love the smell of clean myc. Because of the wintergreen it overpowered it.  Can only wait and see. Has anyone ever used 50 percent?


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OfflinePinPornProducer
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Re: had to do g2g transfers today with 50% isopropyl wintergreen flavor... [Re: DeadPhan]
    #21703302 - 05/20/15 05:45 PM (8 years, 8 months ago)

What are you doing with iso during g2g?


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Offlinemushpunx
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Re: had to do g2g transfers today with 50% isopropyl wintergreen flavor... [Re: PinPornProducer]
    #21703320 - 05/20/15 05:50 PM (8 years, 8 months ago)

I use tons of ISO during G2G, I was my jars/bags, slather my gloves, squirt under the rim etc so I can imagine that being pretty terrible hahah

Ive been thinking about getting a respirator for when I have a lot of work to do


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OfflineDeadPhan
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Re: had to do g2g transfers today with 50% isopropyl wintergreen flavor... [Re: PinPornProducer]
    #21703336 - 05/20/15 05:55 PM (8 years, 8 months ago)

Sanitizing my hands and the jars and lids?  Its pretty common practice. Suprised youve never heard of as much. I was basically taught that that was a pretty golden rule.


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Big Gulps!  Alright!  Well,  See ya later!
And if i claim to be a wise man, well, it surely means that i dont know!


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OfflinePinPornProducer
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Re: had to do g2g transfers today with 50% isopropyl wintergreen flavor... [Re: DeadPhan]
    #21703352 - 05/20/15 06:01 PM (8 years, 8 months ago)

Gotcha, I've never worked with a flow hood. I just wipe everything down with 10% bleach water.


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Offlinemushpunx
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Re: had to do g2g transfers today with 50% isopropyl wintergreen flavor... [Re: PinPornProducer]
    #21703369 - 05/20/15 06:06 PM (8 years, 8 months ago)

Oh dude you shoukd be using tons of ISO in your SAB work too man get a squeeze bottle and some paper towels, wipe everything jars plates gloves
Really slather it on dude!!


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OfflinePinPornProducer
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Re: had to do g2g transfers today with 50% isopropyl wintergreen flavor... [Re: mushpunx]
    #21703387 - 05/20/15 06:11 PM (8 years, 8 months ago)

I work in a sealed glovebox, I wipe all my jars and inside of the box down with the bleach water and load em all in the glove box, give a quick spray of Lysol in the box, shut the lid, wait 15 min then go to work. Works great so far (knock on wood)


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InvisibleMad Season
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Re: had to do g2g transfers today with 50% isopropyl wintergreen flavor... [Re: PinPornProducer]
    #21703412 - 05/20/15 06:17 PM (8 years, 8 months ago)

Bleach, iso, lysol, all are sanitizers and won't completely sterilize things. Really doesn't matter what you choose to use. Alcohol with water is the best at killing things though. Bleach isn't as effective and won't kill as much spores across the range. Imo tho it doesn't really matter too much. It's more dependant on your techniques. If your hand/arm goes over an open plate or jar, it doesn't matter what you used to sanitize it, it's no longer clean.

Lots of people have success without wiping anything down(not a good practice). They just make sure never to hover over anything and work really slow. That's a lot better imo than any amount of sanitizer


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Edited by Mad Season (05/20/15 06:22 PM)


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Offlinemushpunx
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Re: had to do g2g transfers today with 50% isopropyl wintergreen flavor... [Re: PinPornProducer]
    #21703673 - 05/20/15 07:19 PM (8 years, 8 months ago)

Quote:

PinPornProducer said:
I work in a sealed glovebox, I wipe all my jars and inside of the box down with the bleach water and load em all in the glove box, give a quick spray of Lysol in the box, shut the lid, wait 15 min then go to work. Works great so far (knock on wood)





Glovebox? I mean if it works for you it works but dude we gotta get you into a Still Air Box instead man, the sealed arms in a glovebox actually stir up air currents which is a no no!

In SAB you just give a quick spritz with soapy water on the walls (to catch airborn contams), let it settle, wipe everything that goes inside with ISO.

You dont need to sterilize anything, just keep the air still so contams dont land in your open work.. controlled, descicive hand motions... you get the idea.

Still air box! Ahhh!

:rofl:


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OfflinePinPornProducer
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Re: had to do g2g transfers today with 50% isopropyl wintergreen flavor... [Re: mushpunx]
    #21703700 - 05/20/15 07:24 PM (8 years, 8 months ago)

Lol, but I like my glovebox man. We've been through alot together


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Offlinemushpunx
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Re: had to do g2g transfers today with 50% isopropyl wintergreen flavor... [Re: PinPornProducer]
    #21703712 - 05/20/15 07:25 PM (8 years, 8 months ago)

Dude thats like so 2003


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OfflinePinPornProducer
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Re: had to do g2g transfers today with 50% isopropyl wintergreen flavor... [Re: mushpunx]
    #21703721 - 05/20/15 07:26 PM (8 years, 8 months ago)

:unwanted:


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InvisiblebodhisattaMDiscordReddit
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Re: had to do g2g transfers today with 50% isopropyl wintergreen flavor... [Re: mushpunx]
    #21703729 - 05/20/15 07:27 PM (8 years, 8 months ago)

SAB or not you don't need shit loads of iso, use sparingly. a jar should last you a long time, you just wipe the jars off. iso is a precaution it's not a saving grace. if you did things right you wouldn't need it at all it's just a safety blanket.


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InvisibleMad Season
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Re: had to do g2g transfers today with 50% isopropyl wintergreen flavor... [Re: PinPornProducer]
    #21703745 - 05/20/15 07:29 PM (8 years, 8 months ago)

Quote:

PinPornProducer said:
:unwanted:



LOL! Idk man glove box or sab doesn't really matter. They're both way better than open air. If it works for you just keep using it. I was actually thinking of turning one of my sabs to a glove box so you're not alone haha


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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Offlinemushpunx
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Re: had to do g2g transfers today with 50% isopropyl wintergreen flavor... [Re: bodhisatta]
    #21703776 - 05/20/15 07:34 PM (8 years, 8 months ago)

I dunno man my house is sooo full of trich.. Ive had much lower contam rate since I started being a little more liberal with the ISO wipes, Blinding Leaf sugguested I hit the lip of the jar with a syringe of ISO and its made quite a difference!
Maybe not a saving grace but it does help a lot IMO


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InvisiblebodhisattaMDiscordReddit
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Re: had to do g2g transfers today with 50% isopropyl wintergreen flavor... [Re: mushpunx]
    #21703796 - 05/20/15 07:38 PM (8 years, 8 months ago)

Quote:

mushpunx said:
I dunno man my house is sooo full of trich.. Ive had much lower contam rate since I started being a little more liberal with the ISO wipes, Blinding Leaf sugguested I hit the lip of the jar with a syringe of ISO and its made quite a difference!
Maybe not a saving grace but it does help a lot IMO




100 triches or 100000 triches doesn't really matter everyone's got it in their house.


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Offlinesaltycheesecake
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Re: had to do g2g transfers today with 50% isopropyl wintergreen flavor... [Re: bodhisatta]
    #21703798 - 05/20/15 07:38 PM (8 years, 8 months ago)

:cool:


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