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RogerSmith

Registered: 01/29/15 
Posts: 365
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First time with bear's head tooth
#21699076 - 05/19/15 04:35 PM (8 years, 8 months ago) |
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Hello mushroom people,
I never successfully fruited hericium so far, but I bought liquid culture of this mushroom, made spawn and used it to inoculate sawdust. I had 500ml of rye seeds, fully colonized, pins started developing on top, I spawned it to around 3L of PASTEURIZED sawdust.
Now it's been more than a week and I don't see any strong colonization. I have no idea if it is colonized or not, the substrate block is compact but I'm not sure if mycelium bonded it or it is normal compressed sawdust. Grains in substrate look colonized but the white mycelium did not spread very much. I did get first primordia though.
I am considering 2 options. I can let it colonize more and in case it fails, I will detect contamination visually or by smell. Or I could make a hole where mushroom is starting to pin and see what happens.
Here are 2 pictures of what I'm talking about but they won't tell you much I think.


What should I do? If hericium is spawned to pasteurized sawdust, how quick and strong is colonization? Most of the bags I saw that were fruiting were very white.
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Juiceh
Dabbing All Day



Registered: 09/25/12
Posts: 3,208
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Re: First time with bear's head tooth [Re: RogerSmith]
#21700682 - 05/20/15 12:01 AM (8 years, 8 months ago) |
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Substrate looks a bit too wet. Also, doesn't look very colonized from the pics. However that's sorta par for the course with the species. Usually when its about time to fruit the block will not look very colonized, there should be some wispy mycelium visible around the block though. Then the top will start to thicken up with knots & primordia. At that point it's time for fruiting. Doesn't look like your there yet, and that extra visible moisture probably won't help. LM doesn't like to be too wet, especially the fruits.
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RogerSmith

Registered: 01/29/15 
Posts: 365
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Re: First time with bear's head tooth [Re: Juiceh]
#21701128 - 05/20/15 04:29 AM (8 years, 8 months ago) |
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Substrate has proper moisture. Bag is not filled airtight, there is some air between substrate and bag, that's why there is condensation. It doesn't look colonized to me, the only visible colonized parts are those around spawn grains and even that mycelium looks weak, but on the other hand, it could be colonized. I'm confused, that's why I decided to share this and seek different opinion. I think I will put it in fruiting conditions and see what happens next. Whatever happens will contribute to my knowledge of this species.
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mcchieftan
Part man, part mushroom



Registered: 09/08/05
Posts: 525
Loc: Norway
Last seen: 9 months, 1 day
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Re: First time with bear's head tooth [Re: RogerSmith]
#21701242 - 05/20/15 05:58 AM (8 years, 8 months ago) |
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I have a similar situation with grain spawn from a H.e culture that I received. After 10 days there was the tiniest hint of white on the grain and only after perhaps 20 days is everything starting to look white, though nothing like P.e or P.o...
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RogerSmith

Registered: 01/29/15 
Posts: 365
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Re: First time with bear's head tooth [Re: mcchieftan]
#21734289 - 05/28/15 09:05 PM (8 years, 7 months ago) |
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I released that pin from picture, it started to get bigger, but my fruiting area was smelly. So, few days later when I'm doing daily check on the bag I find something green-brown nasty thing, at first it looks like mold, but than I see that mycelium of hericium colored. Than I made a change to my fruiting chamber and decided to rise humidity, next day I checked the fruit turned black/green at bottom part, like that colored thing, and additionally, big patch of trich appeared. I threw it out. I will work only with sterile substrates till I get it figured out.
 
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