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OfflineWydue Wanano
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Coir tub Green Mold, hi-res pictures, question/explanation?
    #21697659 - 05/19/15 09:33 AM (8 years, 8 months ago)

Was originally coming to ask whether my tub was contam'd but I'm now absolutely sure that it is after taking a closer look. I took some really close up pictures with my phone camera + a jeweller's loupe (kinda impressed with this thing) because I noticed there weren't many good reference pictures of the beginnings of green mold on coir that I could find.
I used the 50/50 coir tek with WBS.
I've had problems with green mold before, and I'm pretty certain it's my spawn, as I've had a HEPA filter running in the room nonstop for a week+, and previously I'd just taken whole cakes of WBS and fruited those, which exploded into green mold once they were out of their containers and finished their first flush.
Question 1) Contamination of spawn. The inoculated WBS jars colonized fully without so much as a hint of competing molds, so at what point does spawn become contaminated if not during inoculation or from improper sterilization? I thought my sterile technique was pretty good, got a SAB, a small, closed room I can wipe down easily, and I've had PF jars go swimmingly before.
2) Pasteurization temperatures. I think this is where I went wrong. I didn't have a thermometer (now I do) so I sort of guestimated when the water was ~70*C to toss in my brick of coir. I've read that temperatures above this range will mostly sterilize the bacteria you want in there and make it more susceptible to trich.







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W'sup, I'm a fourth year chem major. Got chemistry questions? Ask me! I know the most about physical and organic chemistry.


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OfflineMatt87
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Re: Coir tub Green Mold, hi-res pictures, question/explanation? [Re: Wydue Wanano]
    #21697687 - 05/19/15 09:42 AM (8 years, 8 months ago)

I've had issues as of late, so I'm going to monitor this thread.


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Once you understand the way broadly, you see it in all things. -Musashi


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Offlinethebitterbuffalo26
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Re: Coir tub Green Mold, hi-res pictures, question/explanation? [Re: Matt87]
    #21697724 - 05/19/15 09:59 AM (8 years, 8 months ago)

Do you shake your jars of grain? I usually won't notice a grain contamination until after i shake it.  Also,  coir shouldn't contaminate easily. I have an open topped tub with grain and coir in my room at this moment and it's not contaminated and we have three cats and two dogs etc. If i had coffee ground in it though it's more likely to contaminate.


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OfflineWydue Wanano
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Re: Coir tub Green Mold, hi-res pictures, question/explanation? [Re: thebitterbuffalo26]
    #21698198 - 05/19/15 12:18 PM (8 years, 8 months ago)

I do shake my jars, and I chose coir for this because of it's contam resistance as it's my first forserious grow following PF tek. I'm planning to let it run for a while in a different room, then cut it open to see whether the mold appears in the middle or just the outside. This tub was the one I fruited the straight WBS to, so I'm thinking if the mold is just on the outside then I probably just had tons of green mold spores leftover inside the tub, which would be annoying because I rubbed it down with alcohol and then Lysol'd the SHIT out of it after that happened.

The mold is also all along the underside of the tub, which wouldn't have had fresh air reaching it even if it was opened, so no matter what, seems the contam either followed my sub in or was in the tub already.


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W'sup, I'm a fourth year chem major. Got chemistry questions? Ask me! I know the most about physical and organic chemistry.


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InvisibleMad Season
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Re: Coir tub Green Mold, hi-res pictures, question/explanation? [Re: Wydue Wanano]
    #21698287 - 05/19/15 12:46 PM (8 years, 8 months ago)

I'm sorry sir but a proper field capacity, properly pasteurized bulk will fight off contams like trich for a long ass time. Trich hitting is always because of dirty spawn if you pasteurized properly. I find pasteurization at 140-160 for 1 hour is best. 90 minutes gives me a lot more contamination because of the more beneficial microbes I killed. Whether the trich was in the jar or hidden molds weakened the spawn enough to let trich germinate on the surface, it still goes down to dirty spawn.

Wbs is quite starchy and as such is FILLED with bacterial endospores. I found pcing for 2 hours did WAY better.

Guess which one I pcd for 90 minutes and which one was 120 minutes:



Look there's still wet looking grains on the bottom, indicating bacteria still even at a 120 minute pc.

Although many other factors could cause it, ever since I upped my pressure cook times to 2 hours I've been having a way higher success rate. Hope this helps.


Edited by Mad Season (05/19/15 12:54 PM)


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OfflineWydue Wanano
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Re: Coir tub Green Mold, hi-res pictures, question/explanation? [Re: Mad Season]
    #21699417 - 05/19/15 05:57 PM (8 years, 8 months ago)

I'll definitely try upping the sterilization time for the next round and seeing if that does anything.
Noticing now that the only places growing it are where the spawn is mixed in, patches of straight coir are clean, so final verdict is you're right, it is my spawn. I might've under or oversoaked my grains too, who knows, but I am armed with advice for next time, thanks erryone :thumbup:


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W'sup, I'm a fourth year chem major. Got chemistry questions? Ask me! I know the most about physical and organic chemistry.


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InvisibleTheEaglesGift
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Re: Coir tub Green Mold, hi-res pictures, question/explanation? [Re: Wydue Wanano]
    #21703454 - 05/20/15 06:29 PM (8 years, 8 months ago)

I had bacteria issues with wbs at 90 minutes as well, that 120 minutes took care of.

To parrot what others have said, if you are properly pasteurizing and seeing green before first flush, then your spawn was likely dirty.


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OfflineWydue Wanano
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Re: Coir tub Green Mold, hi-res pictures, question/explanation? [Re: TheEaglesGift]
    #21705909 - 05/21/15 10:50 AM (8 years, 8 months ago)

Oh my gOD OH MY GOD I FIGURED IT OUT
I was using the goddamn wrong weight on my pressure cooker valve. Were supposed to be three included, 5/10/15psi, only had two, heavier one was shaped like the 15psi weight so I sort of assumed. Because I own a scale now, I just weighed the two, and the heavier one is twice the weight of the lighter one, meaning I've been cooking at 10psi this whole time. Absolutely elementary, goddamn.


--------------------
W'sup, I'm a fourth year chem major. Got chemistry questions? Ask me! I know the most about physical and organic chemistry.


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InvisibleTheEaglesGift
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Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
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Re: Coir tub Green Mold, hi-res pictures, question/explanation? [Re: Wydue Wanano]
    #21706243 - 05/21/15 12:47 PM (8 years, 8 months ago)

Well, that's an easy problem to fix at least.


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