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OfflineStraya
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Registered: 03/04/12
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How to clean a wild print
    #21697340 - 05/19/15 06:59 AM (8 years, 8 months ago)

Hey guys, so I've been hunting the search function for the last hour and had fuck all luck.

I've got a cyan print which I need to clean. What i've read is basically you transfer the spores to agar, then cut out wedges from non infected sections and transfer to fresh agar until you have a fresh culture?

Is there a tek for this somewhere or is that it?

My other question is once you clean a print on agar and have an agar dish with mycelium, is it somehow possible to then transfer some of the mycelium to a LC? The reason i ask is i'd like to make LC's so i could inoculate cakes with the specimen that was once contaminated. I just tried it and have had 100% contam rate so want to find out how to clean up the front and then get it into a syringe.

Thanks for the help guys, just having no luck with the search function :frown:


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Invisiblegrainbrain
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Registered: 05/11/11
Posts: 2,626
Re: How to clean a wild print [Re: Straya]
    #21697350 - 05/19/15 07:05 AM (8 years, 8 months ago)

Keep transferring from the leading edge until your plates grow out clean.

You can drop a clean wedge into LC, but you need to use a SAB or flowhood and be sure of your sterile procedure.


--------------------
Stop cold shocking your mycelium!
Hot Spawn - Get It On



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OfflineStraya
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Re: How to clean a wild print [Re: grainbrain]
    #21697370 - 05/19/15 07:15 AM (8 years, 8 months ago)

Quote:

grainbrain said:
Keep transferring from the leading edge until your plates grow out clean.

You can drop a clean wedge into LC, but you need to use a SAB or flowhood and be sure of your sterile procedure.




Awesome, that's exactly what I was after. Thanks so much mate - leaving you 5 shrooms now :smile:


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InvisibleGrey
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Registered: 11/06/14
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Re: How to clean a wild print [Re: Straya]
    #21697394 - 05/19/15 07:26 AM (8 years, 8 months ago)

Check this out:

https://www.shroomery.org/forums/showflat.php/Number/18430998

And for the LC, just drop a clean wedge into your LC in a clean environment.


--------------------


:takingnotes:  AMU Q&A  :takingnotes:


If you don't have a plan of your own, you'll become a part of somebody else's.


Edited by Grey (05/19/15 07:28 AM)


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OfflineStraya
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Re: How to clean a wild print [Re: Grey]
    #21697403 - 05/19/15 07:29 AM (8 years, 8 months ago)

Quote:

Grey said:
Check this out:

https://www.shroomery.org/forums/showflat.php/Number/18430998

And for the LC, just drop a clean wedge into your LC in a clean environment.




Perfect :smile:

Thanks mate i'd leave you a rating but I'm pretty sure I've already left you like one and added an additional edit to it :wink:

Scratch that I checked and i hadn't so now I have :smile: Cheers mate


Edited by Straya (05/19/15 07:31 AM)


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InvisibleGrey
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Re: How to clean a wild print [Re: Straya]
    #21697546 - 05/19/15 08:31 AM (8 years, 8 months ago)

Thank you sir. We're you going to crumble the cakes, or top fruit?


--------------------


:takingnotes:  AMU Q&A  :takingnotes:


If you don't have a plan of your own, you'll become a part of somebody else's.


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OfflineStraya
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Re: How to clean a wild print [Re: Grey]
    #21697583 - 05/19/15 08:59 AM (8 years, 8 months ago)

Probably do a bit of both, crumble some to mono and then probably try run some like the pf tek


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InvisibleGrey
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Re: How to clean a wild print [Re: Straya]
    #21697611 - 05/19/15 09:15 AM (8 years, 8 months ago)

I don't know how well they'll do as cakes. However, search "pan cakes" by a member named MacMerdin. Pretty good read


--------------------


:takingnotes:  AMU Q&A  :takingnotes:


If you don't have a plan of your own, you'll become a part of somebody else's.


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