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OfflinefirstTIMER420
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BRF/PF colonization
    #21696817 - 05/19/15 12:20 AM (8 years, 8 months ago)

hey everyone, I guess you could say im in the beginning of my first grow, and im thinking that my colonization rate is a little slow, im on the end of day 4, and I cannot see any mycelium growth or anything of the sort, I don't think I see any contams either, I will be posting pics tomorrow on this same thread, but was wondering what the average time using BRF cakes/B+ strain of first signs of colonization were.

-thanks :rail2:


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OfflineMatt87
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Re: BRF/PF colonization [Re: firstTIMER420]
    #21696832 - 05/19/15 12:29 AM (8 years, 8 months ago)

Its fine! I wouldn't even look at them for 7 to ten more days, just read up.


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OfflinefirstTIMER420
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Re: BRF/PF colonization [Re: Matt87]
    #21696880 - 05/19/15 12:49 AM (8 years, 8 months ago)

ok man I gotcha, from what ive read, a lot of people using this tek see signs of colonization from 4 days on, that's why I was wondering.


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OfflineMatt87
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Re: BRF/PF colonization [Re: firstTIMER420]
    #21697695 - 05/19/15 09:46 AM (8 years, 8 months ago)

At 4 to seven days, it will look like a faint white spot or two in the jar.


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OfflineAdept
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Re: BRF/PF colonization [Re: firstTIMER420]
    #21697698 - 05/19/15 09:48 AM (8 years, 8 months ago)

Quote:

firstTIMER420 said:
ok man I gotcha, from what ive read, a lot of people using this tek see signs of colonization from 4 days on, that's why I was wondering.




I wait at least two weeks before I concern myself with even looking at the jars.


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InvisibleMad Season
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Re: BRF/PF colonization [Re: Adept]
    #21698818 - 05/19/15 03:26 PM (8 years, 8 months ago)

I normally keep checking daily during the first 3 days to make sure no contaminations come. They usually do for the most part during then. After I just don't look for a week or 2.


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OfflineAdept
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Re: BRF/PF colonization [Re: Mad Season]
    #21698907 - 05/19/15 03:51 PM (8 years, 8 months ago)

Quote:

Mad Season said:
I normally keep checking daily during the first 3 days to make sure no contaminations come. They usually do for the most part during then. After I just don't look for a week or 2.




What do you do if you find a contaminated jar?


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InvisibleMad Season
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Re: BRF/PF colonization [Re: Adept]
    #21698913 - 05/19/15 03:53 PM (8 years, 8 months ago)

Goes into my contamination pile, wash out the jar and... MAKE MOREE! :wink: lol


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How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
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AMU Q&A
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OfflineAdept
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Re: BRF/PF colonization [Re: Mad Season]
    #21698948 - 05/19/15 04:04 PM (8 years, 8 months ago)

Quote:

Mad Season said:
Goes into my contamination pile, wash out the jar and... MAKE MOREE! :wink: lol




See, that's why I don't bother to look for two weeks...  No matter how far along the contamination growth is my SFD on top should keep it inside the jar.  At the 2 week mark I find ALL the jars with any contamination and PC them all together for 1.5 hours to make sure anything inside the jar is DEAD DEAD DEAD before I open & pitch the contents (bury in my yard). Then, and only then, will I wash them by hand scrubbed with Stainless pad and store away.


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InvisibleMad Season
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Re: BRF/PF colonization [Re: Adept]
    #21698989 - 05/19/15 04:12 PM (8 years, 8 months ago)

Probably true. I just like it because it's more efficient :wink: but I'm also a crazy ocd guy in this hobby lol!


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
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OfflineAdept
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Re: BRF/PF colonization [Re: Mad Season]
    #21699001 - 05/19/15 04:15 PM (8 years, 8 months ago)

Quote:

Mad Season said:
Probably true. I just like it because it's more efficient :wink: but I'm also a crazy ocd guy in this hobby lol!




In the battle of who is more of an "OCD guy" I would just say I'd never open a contaminated jar to the open air without destroying whatever was living inside first.  Been down that road, one wrong jar and your lab is destroyed!


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InvisibleMad Season
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Re: BRF/PF colonization [Re: Adept]
    #21699044 - 05/19/15 04:25 PM (8 years, 8 months ago)

I have to kindly disagree. A walk in the forest will expose you to more spores than a contaminated jar ever would. Just the act of going into the lab would contaminate it if you had it sterile all the time.

If you can work with agar to make a clean inoculation, Pc for 2 hours with proper prep, you SHOULD have clean spawn/cakes. Once it colonizes cleanly it should be strong enough to fight whatever you contaminate when you simply enter the room. Fruiting will expose it to millions of spores of trich as well as everything else. I always blame the jar/my prep/techniques for contaminating in fruiting rather than the room. It's basically impossible to make it a clean room.

I agree keeping as much contamination spores out is a great idea but it's not what's going to mess everything up. A properly made jar will keep contams out just like how you said it can keep contams in. So if it contaminates it's because the mycelium shit the bed. Agar has made everything way more successful in my experience, and as you can see I'm not PERFECTLY clean. Just only when I'm in the SAB.


Edited by Mad Season (05/19/15 04:31 PM)


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OfflineAdept
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Re: BRF/PF colonization [Re: Mad Season]
    #21699088 - 05/19/15 04:38 PM (8 years, 8 months ago)

:shrug:
I don't disagree with your assessment, however I have two points in response:


1) I have no way to PC a forest to make sure it's clean before I walk through it.

2) I CAN PC a contaminated jar before I open it.

I guess it's all about options...


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InvisibleMad Season
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Re: BRF/PF colonization [Re: Adept]
    #21699151 - 05/19/15 04:52 PM (8 years, 8 months ago)

Haha I'm not saying to not pc a contamination. That's a great practice. I'm just pointing out when you see a contamination it isn't the end of your grow room and you need to bleach it all again. If you have a good technique you're confident in, you get a contamination in just 1 jar every so often and you're like oh.. :shrug: shit happens. I've yet to be set back by contams since going to agar. Definitely 1 less thing to worry about to have clean inoculation every time


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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OfflinefirstTIMER420
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Re: BRF/PF colonization [Re: Mad Season]
    #21700740 - 05/20/15 12:26 AM (8 years, 8 months ago)

also, by the way I used a very small drill bit to make the innoc holes, the needle BARELY fits in. will that mess up the proper amount of gas exchange its supposed to have? ive been seeing you should use 1/4" holes for the innoc holes. since mine are smaller than usual, ive been thinking I should take the tape off.. also SFD?


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OfflineAdept
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Re: BRF/PF colonization [Re: firstTIMER420]
    #21701373 - 05/20/15 07:13 AM (8 years, 8 months ago)

Synthetic Filter Disc


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Invisiblewowimflabbergasted
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Re: BRF/PF colonization [Re: firstTIMER420]
    #21701406 - 05/20/15 07:27 AM (8 years, 8 months ago)

Quote:

firstTIMER420 said:
also, by the way I used a very small drill bit to make the innoc holes, the needle BARELY fits in. will that mess up the proper amount of gas exchange its supposed to have?



No.


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OfflinefirstTIMER420
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Re: BRF/PF colonization [Re: firstTIMER420]
    #21707658 - 05/21/15 07:43 PM (8 years, 8 months ago)

Quote:

firstTIMER420 said:
hey everyone, I guess you could say im in the beginning of my first grow, and im thinking that my colonization rate is a little slow, im on the end of day 4, and I cannot see any mycelium growth or anything of the sort, I don't think I see any contams either, I will be posting pics tomorrow on this same thread, but was wondering what the average time using BRF cakes/B+ strain of first signs of colonization were.

-thanks :rail2:




here it is yall, day 5, these were taken earlier today, and every day I can see noticeable growth in the size of the white blotches, they look healthy from what ive seen.  the first two pics are pretty clear, the rest kinda suck lol, but you can see the white spots if you look.  so far, I don't think I see any contams, and im taking that as a good sign.  Taking it day by day at this point :shrug:

Let me know what yall think(healthy not healthy, contams, if they are where they should be etc.)



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Invisiblewowimflabbergasted
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Re: BRF/PF colonization [Re: firstTIMER420]
    #21707960 - 05/21/15 08:52 PM (8 years, 8 months ago)

Try and consolidate your log into a single thread.


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