Home | Community | Message Board

Sporeworks
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore Injection Grain Bag   Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Liquid Cultures   PhytoExtractum Kratom Powder for Sale

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineIwillnevertell
The One
 User Gallery
Registered: 07/27/14
Posts: 71
Last seen: 7 years, 7 months
any sectors in this agar?
    #21695646 - 05/18/15 07:21 PM (8 years, 8 months ago)

Im having trouble identifying sectors in my myc on agar. Heres an example of my best one that i have. This is after the first transfer from the original multispore agar. So do i need to keep isolating further or whats your opinion. Thanks



Extras: Filter Print Post Top
Offlinethebitterbuffalo26
Fartyr
Male User Gallery


Registered: 04/18/15
Posts: 555
Loc: Texas Flag
Last seen: 7 years, 7 months
Re: any sectors in this agar? [Re: Iwillnevertell]
    #21695655 - 05/18/15 07:23 PM (8 years, 8 months ago)

I would go for the 8:30 to 12:00 area


--------------------


Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,810
Loc: Canada Flag
Trusted Cultivator
Re: any sectors in this agar? [Re: thebitterbuffalo26]
    #21695717 - 05/18/15 07:37 PM (8 years, 8 months ago)

Ya got a few sectors still there. The good news is your close. If you can take really small slices ya should have isolates. Aim for the individual ropes if you can. Then carefully label them and fruit them all. Then hopefully ya get one that is awesome, throw all the crappy ones away and fruit the good one forever :thumbup:


Extras: Filter Print Post Top
OfflinecronicrFacebook
 User Gallery


Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 8 days
Trusted Cultivator
Re: any sectors in this agar? [Re: Pastywhyte]
    #21695738 - 05/18/15 07:47 PM (8 years, 8 months ago)

Quote:

Pastywhyte said:
Ya got a few sectors still there. The good news is your close. If you can take really small slices ya should have isolates. Aim for the individual ropes if you can. Then carefully label them and fruit them all. Then hopefully ya get one that is awesome, throw all the crappy ones away and fruit the good one forever :thumbup:



:whathesaid: take a couple or more chunks to test out and good luck!


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


Extras: Filter Print Post Top
OfflineIwillnevertell
The One
 User Gallery
Registered: 07/27/14
Posts: 71
Last seen: 7 years, 7 months
Re: any sectors in this agar? [Re: cronicr]
    #21695765 - 05/18/15 07:55 PM (8 years, 8 months ago)

Thanks just making sure i was on the right track. I fruited ms before and potency was just pathetic. So im hoping this agar work cranks it up.


Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,810
Loc: Canada Flag
Trusted Cultivator
Re: any sectors in this agar? [Re: Iwillnevertell]
    #21695778 - 05/18/15 08:00 PM (8 years, 8 months ago)

With potency its basically just try until ya find what ya want. Most cubes are pretty average, I eat about 6 grams or so to get a good trip going. But with really potent fruits I only need 1-1.5. But it can take some time to find that. So be patient. If your isolates fail you (not saying they will but nothing is guaranteed) try cloning. Its faster and easier to get an idea of what your gonna end up with.


Extras: Filter Print Post Top
Offlinemushpunx
Fungus Punk
 User Gallery


Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 14 hours
Trusted Cultivator
Re: any sectors in this agar? [Re: Pastywhyte]
    #21695858 - 05/18/15 08:17 PM (8 years, 8 months ago)

Is that really second transfer from germination?


--------------------

Amateur Mycologists United
AMU Q&A


Extras: Filter Print Post Top
OfflineYaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 11 days
Re: any sectors in this agar? [Re: mushpunx]
    #21696004 - 05/18/15 08:46 PM (8 years, 8 months ago)

I'm convinced, going to do agar in the next few weeks. From spore or from tissue sample?


Extras: Filter Print Post Top
OfflinecronicrFacebook
 User Gallery


Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 8 days
Trusted Cultivator
Re: any sectors in this agar? [Re: YaMoonSun]
    #21696028 - 05/18/15 08:51 PM (8 years, 8 months ago)

or both:shrug:


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


Extras: Filter Print Post Top
Offlinemushpunx
Fungus Punk
 User Gallery


Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 14 hours
Trusted Cultivator
Re: any sectors in this agar? [Re: YaMoonSun]
    #21696057 - 05/18/15 08:57 PM (8 years, 8 months ago)

Quote:

YaMoonSun said:
I'm convinced, going to do agar in the next few weeks. From spore or from tissue sample?




Go for both. If you have something clone worthy like a nice cluster take clones yes!

Also knock up some plates from spores and clean them up for spawn, but keep the culture going to work on isolating

Cron beat me to it!


--------------------

Amateur Mycologists United
AMU Q&A


Edited by mushpunx (05/18/15 08:57 PM)


Extras: Filter Print Post Top
OfflineYaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 11 days
Re: any sectors in this agar? [Re: cronicr]
    #21696085 - 05/18/15 09:03 PM (8 years, 8 months ago)

Learn from experience and widen my horizon? Possibly.


Extras: Filter Print Post Top
OfflinecronicrFacebook
 User Gallery


Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 8 days
Trusted Cultivator
Re: any sectors in this agar? [Re: mushpunx]
    #21696090 - 05/18/15 09:05 PM (8 years, 8 months ago)

Quote:

mushpunx said:


Cron beat me to it!



you yak too much:laugh2:


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


Extras: Filter Print Post Top
OfflineYaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 11 days
Re: any sectors in this agar? [Re: mushpunx]
    #21696117 - 05/18/15 09:09 PM (8 years, 8 months ago)

I feel like I have some really neat fruits from spore syringes, and I wanna replicate them, but I'm not sure if a LC is going to contain the same potential as multiple sectors - If that makes sense. Like, sure, similar characteristics, but they can't be dead-on, can they? Or is that why I'm aiming for a single sector isolate?


Edited by YaMoonSun (05/18/15 09:28 PM)


Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,810
Loc: Canada Flag
Trusted Cultivator
Re: any sectors in this agar? [Re: YaMoonSun]
    #21696299 - 05/18/15 09:47 PM (8 years, 8 months ago)

Wow I missed that this was only a single transfer. OP is lucky to have such limited genetics that quick. Might be some more tangled in there than it looks like at first but isolates are not far off. Well done.

With ms there is the potential for anything. Isolates are even more extreme.  Many won't perform well, some might not fruit at all. But one in ten will be killer. So when ya find that one keep it.


Extras: Filter Print Post Top
OfflinecronicrFacebook
 User Gallery


Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 8 days
Trusted Cultivator
Re: any sectors in this agar? [Re: Pastywhyte]
    #21696325 - 05/18/15 09:53 PM (8 years, 8 months ago)

what was your recipe? maybe just starving myc lol


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


Extras: Filter Print Post Top
Offlinemushpunx
Fungus Punk
 User Gallery


Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 14 hours
Trusted Cultivator
Re: any sectors in this agar? [Re: cronicr]
    #21696330 - 05/18/15 09:54 PM (8 years, 8 months ago)

Quote:

cronicr said:
Quote:

mushpunx said:


Cron beat me to it!



you yak too much:laugh2:





Hahaha hell yeah I do
It take me a paragraph to say anything :lol:


Yeah thats freaking awesome you have sectors showing in so few transfers!!


--------------------

Amateur Mycologists United
AMU Q&A


Extras: Filter Print Post Top
OfflineIwillnevertell
The One
 User Gallery
Registered: 07/27/14
Posts: 71
Last seen: 7 years, 7 months
Re: any sectors in this agar? [Re: mushpunx]
    #21697188 - 05/19/15 05:16 AM (8 years, 8 months ago)

Yea its only the first transfer from ms and i have 6 others with similiar results. I guess thats why i was asking about sectoring coz i wasnt expecting such good results. Thanks feels good to be lucky haha


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: North Spore Injection Grain Bag   Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Liquid Cultures   PhytoExtractum Kratom Powder for Sale


Similar ThreadsPosterViewsRepliesLast post
* agar transfer substitution? omniscientlore 1,478 3 12/19/22 07:14 AM
by Screwup
* agar to agar transfer ohmatic 15,622 11 01/12/21 09:00 PM
by bodhisatta
* How to make agar 2 agar transfer? esp 6,655 7 01/08/04 11:08 AM
by YidakiMan
* agar transfers *DELETED* thisone 1,245 3 11/20/03 08:58 PM
by Mycomancer
* agar to agar / g2g degeneration effects ohmatic 2,621 11 01/17/05 03:46 PM
by MushroomFriend
* Pasty agar plates, how does this mycelium look?
( 1 2 all )
cheesemaster 1,247 31 03/02/18 07:20 PM
by cheesemaster
* substitute for agar? brewwhaha1 2,257 15 02/19/05 09:27 PM
by MontyRev
* Innoculation with agar wedge Visigoth 5,278 6 01/15/23 12:59 PM
by Screwup

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
1,619 topic views. 35 members, 194 guests and 25 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.035 seconds spending 0.01 seconds on 14 queries.