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OfflineMushSicle
Popsicle
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Registered: 04/14/15
Posts: 58
Last seen: 8 years, 6 months
Grow log - P. cubensis "Golden Teacher" and "Bermuda" V-tek invitro * 1
    #21693642 - 05/18/15 08:02 AM (8 years, 8 months ago)

Spores - Golden Teacher and Bermuda. Used a ms syringe for both grows.

Spawn - untreated rye grass seed.

No casing used as per v-tek instructions.

Spawn preparation
Boiled 16 cups water, 4 cups grass seed at a rolling boil for 25 mins. Strained and used 250ml - 1cup grass seed per container - 8, one quart ziplock containers.

Sterilization Method
  Loaded 4 quart jars into pressure cooker and sterilized at 15 psi for 50 mins. Did 4 on Monday morning and 4 on Tuesday morning. Lids were loose on zip locks to avoid explosions and/or container deformities. Left in pressure cooker for at least 8 hours to cool. The jars remained in pressure cooker until removed right next to still air box and immediately sealed tight. Entire area surrounding still air box, inside and arm holes sprayed with disinfectant and allowed to sit 15 mins. Gloves and mask used, wore only my skivvies :wink: and freshly showered. Gloves wiped down with cotton ball and iso alcohol 70%.

Inoculation method
  Ms syringe, very lightly cracked lid and shot 1cc - more or less :wink: into opening and immediately sealed. 1 quart done at a time. After each inoculation the syringe was flamed until red hot.

Placed the jars in my music room closet on a high shelf. So I could serenade them bitches to grow nice healthy mushies.

Monday 5/11 - 4 jars sterilized, inoculated 4 quart jars with GT.

Tuesday 5/12 - 4 jars sterilized, inoculated 4 quart jars with Bermuda.

Friday 5/15 - First myc. growth for GT!

5/17 - more GT growth


5/18 - first signs of Bermuda growth
Pics coming soon.

5/20 update - GT colonizing quickly, Bermuda slowly colonizing. First 2 are GT, last one is Bermuda.





Edited by MushSicle (05/20/15 05:08 PM)


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