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Invisiblevatman
I'm Vatman

Registered: 04/17/14
Posts: 1,642
Oyster and Lions mane project/grow log
    #21687741 - 05/16/15 05:42 PM (8 years, 8 months ago)

Bought a blueoyster starter pack off amazon

http://www.amazon.com/gp/product/B00N190V6K/ref=oh_aui_detailpage_o02_s00?ie=UTF8&psc=1


So far I have transferred some samples from the fully colonized block in the mail to agar plates.
These all grew mycelium I think...


Just about every dish from this pack contained bacteria. I also eberbach the rest of the block. I may have made it too muddy and put it into bulk straw and  coir. I saw some cobweb in it all ready and no mycelium growth. I did fall asleep during the pasteurization but I have it auto maintained to 145F-150F

I got a Lions mane from outgrow.
http://out-grow.com/fungi-cultures-c-2/10ml-liquid-culture-syringes-lions-mane-hericium-erinaceus-p-275.html

I first applied the LC to some dishes. Been a week most have no growth but some do and the ones that do have some bacteria contamination.


I can't tell if the white fluf is mane or mold. Some input on that before I transfer them over to new dishes would be great!


Edited by vatman (07/15/15 10:01 PM)


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OfflineHumbled
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Re: Oyster and Lions mane project/grow log [Re: vatman]
    #21695679 - 05/18/15 07:28 PM (8 years, 8 months ago)

The top pic has what looks to me like good mycelium for a transfer. I cant tell much in the 2nd pic.

The 3rd pic looks like it needs a few more days to show you what it has to offer.:grin::thumbup:


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Invisiblevatman
I'm Vatman

Registered: 04/17/14
Posts: 1,642
Re: Oyster and Lions mane project/grow log [Re: Humbled]
    #21696820 - 05/19/15 12:22 AM (8 years, 8 months ago)

I've transferred all on sat morning. So far no sings of contamination following forward. Some of the dishes of lions mane that showed no growth are now showing sings of growth too.


-------------------------------update------------------------------
One lion's dish has carried forward bacteria, it was one I had issue getting the agar off the knife. Also the lions mane growth seems rather slow compared to the oyster. I have little growth on the lions mane


Edited by vatman (05/19/15 04:39 PM)


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Invisiblevatman
I'm Vatman

Registered: 04/17/14
Posts: 1,642
Re: Oyster and Lions mane project/grow log [Re: vatman]
    #21711507 - 05/22/15 05:45 PM (8 years, 8 months ago)

Getting my work area ready.


From the 2nd transfers I have gotten a few that are a single colony with amazing growth for a week.

The lion's main growth is still kinda odd. It seems to be externally slow too. I have got one that has a flat growth on it I think. About all of the transfers I made from the first dish have not started to grow on the 2nd dish. Just kinda fluffed out a little. More pictures to come of that later.

Eberched a few dishes of agar, dear god I have soo much oyster myc left in it. I covered it back up with tinfoil and put it in the fridge. After these grains take I'm going attempt to use more from the same batch.

Transferred some of the king oyster to petri dishes also grain transferred to some grains too. Washed the entire bag with 70% isopropyl before opening it.


Edited by vatman (07/15/15 10:02 PM)


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Invisiblevatman
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Registered: 04/17/14
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Re: Oyster and Lions mane project/grow log [Re: vatman]
    #21933917 - 07/12/15 03:14 PM (8 years, 6 months ago)

Took a break got back into it a few weeks ago. Put a lot of my plates into the fridge and they mostly got contaminated. Did grain to grain transfers of my jars and setup some straw logs.


I think I'm good but I broke up the fully colonized grains with my hands that were fairly clean at the time but I'm a tad worried that may have ruined them. I pasteurized straw for 1 hour @ 145-150F I didn't add hydrated lime to the straw... Not sure if that is essential


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Offlinenaughtywifeaz
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Registered: 07/12/15
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Re: Oyster and Lions mane project/grow log [Re: vatman]
    #21933962 - 07/12/15 03:24 PM (8 years, 6 months ago)

Interesting :smile:


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Invisiblevatman
I'm Vatman


Registered: 04/17/14
Posts: 1,642
Re: Oyster and Lions mane project/grow log [Re: naughtywifeaz]
    #21934073 - 07/12/15 03:53 PM (8 years, 6 months ago)

Here is some pictures.



Grain transfer next to a blender sample. My blue oyster myc is rather weak. I suspect that its been transferred too many times before I got it.


Bulk grains for my logs.
2/3 failed.The two that failed had too high moisture content and to top it off the weak genetics of the blue oysters.
This picture was from the same batch it did great and colonized within a weak from the king oysters.


Chopping some straw.


A 5 gallon 1000W bucket heater. A temperature controller that is extremely easy to program got off amazon and two water current thingies to ensure even temperature in the cooler.



Ended up wrapping in a blanket to help hold the temperature.




Straw logs had holes poked into them with a scapulae. Room where they are stored currently has 54% RH


Edited by vatman (07/12/15 03:56 PM)


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Invisiblevatman
I'm Vatman


Registered: 04/17/14
Posts: 1,642
Re: Oyster and Lions mane project/grow log [Re: vatman]
    #21944200 - 07/14/15 09:59 PM (8 years, 6 months ago)

I'm worried that my moisture content may be too high. Should I be poking some larger holes into the bags? Also should I burp them?






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OfflineGr0wer
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Re: Oyster and Lions mane project/grow log [Re: vatman]
    #21944690 - 07/14/15 11:52 PM (8 years, 6 months ago)

Did you have a control agar plate open while working? You might just have a bad work environment for agar. My blue oysters started from that amazon source as well. I did a GTG from the sawdust to WBS and got good results. I wouldnt say they had weak genetics, pretty fast for me at least. Not as fast as my phoenix oysters but i think there a faster variety. For colonizing just let it be. If you have holes poked every 6" or so you should be fine. Colonization only needs a little gas exchange, you don't need or want to pump fresh air in there. What is your fruiting chamber going to be?

What did you use that blender for? That could be a source of contam. How do you sterilize it?


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Invisiblevatman
I'm Vatman


Registered: 04/17/14
Posts: 1,642
Re: Oyster and Lions mane project/grow log [Re: Gr0wer]
    #21947322 - 07/15/15 01:11 PM (8 years, 6 months ago)

The blender is an eberbach blender. I didn't use it for the bulk. The bulk contamination came from too much water. The walls of the tub were sweating.

I don't usethe blender that much yet. I'm still experimenting it. So far what I have used it for had sauces. I cover the top with tin foil and put the whole thing into a pressure cooker for 30 minutes. Of course minus the base. I do three two second bursts when I use it. The grains I used it on I broke up on Monday. I'll get some pictures when I get home


Edited by vatman (07/15/15 01:14 PM)


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Invisiblevatman
I'm Vatman


Registered: 04/17/14
Posts: 1,642
Re: Oyster and Lions mane project/grow log [Re: vatman]
    #21949557 - 07/15/15 09:55 PM (8 years, 6 months ago)

Only 1 of the 6 jars with the blender failed.



Failed jar as you can see a section in this following picture where bacteria took over.





The following are the Lion's mane. The myc is hard to see with these guys.
As far as I can tell there is 0 fail with them



What I dislike about these guys since I'm starting out with them. I find it hard tell when its ready to transfer these suckers.

Also from what I have been reading Laitake and Shiitake will only fruit on wood. Lion's mane will fruit on straw but wont be as good.

So I'm looking for cheap sources of wood. From what I read hardwood fuel pellets work well. I also saw bulk aspen bedding at a feed store a year back. I'm going to give them a call as its a 50 min trip one way to them same to hardwood stores etc. I live in kinda in the middle of no where. Closet thing I've been able to find around the local area is tree bark witch can only be supplemented up to 20% from what I have read.. I have come across downed trees that "could be used"

When growing indoors from grain to wood do you want to pasteurize or sterilize the wood?

------Edit-----
Also reuploaded some of the large images from the first two posts to the shroomery. The overall QL is lower but not that noticeable.


Edited by vatman (07/15/15 10:14 PM)


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OfflineGr0wer
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Re: Oyster and Lions mane project/grow log [Re: vatman]
    #21950886 - 07/16/15 08:18 AM (8 years, 6 months ago)

Yes you want to pasteurize the mulch with heat 140-150 core temp for 60-90min. I sourced free mulch from my local town recycle center, you can also look for nearby wood mills or cabinet/furniture makers using hard woods. Look around before you spend any money on mulch or sawdust. With lions mane my reading found that coco coir is also good substrate for it, i currently have a grow bag colonizing in coir.


Edited by Gr0wer (07/16/15 08:24 AM)


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InvisibleMykes logos
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Re: Oyster and Lions mane project/grow log [Re: Gr0wer]
    #21951087 - 07/16/15 09:27 AM (8 years, 6 months ago)

I wouldn't be surprised if all of your bags stall and you start seeing mean green and bacteria in them.

I noticed your lab work lacked some good aseptic conditions, besides the fact that I find the origin and cleanliness of your master cultures questionable.

Your straw pasteurization was without any thing that changes the ph and it was kind of low (you wanna be closer to 160, not 140)...

The blender thing also increases my skepticism of you getting fruit of those logs.

However... regardless of the success of this grow fruit-wise, you're doing an excellent job and are obviously on the right track!

You're very creative. Check your aseptic lab techniques and keep up the good work- good luck with the straw logs!


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Invisiblevatman
I'm Vatman


Registered: 04/17/14
Posts: 1,642
Re: Oyster and Lions mane project/grow log [Re: Mykes logos]
    #21951260 - 07/16/15 10:09 AM (8 years, 6 months ago)

My logs are doing fine. I poked some more holes into them. After reviewing what other people have done. My logs didn't have large enough holes to allow excessive moisture to escape. Pasteurization starts at 140. My ranges are 145-152

Quote:

RogerRabbit said:
Maintaining an internal substrate temperature of 140F to 160F for 60 to 90 minutes will give effective pasteurization.
RR




My tap water has a PH of 7.9 witch is rather high. Hydrated lime has a PH of 12 witch is diluted into water the raise the PH. Overall my logs look fine and almost ready. I'm still worried about the excessive water in them. I didn't follow the

From the plates I did 2-3 transfers before I went to grain.

I have also moved my lab in the mean time. I moved it outside of my room that had a swamp cooler into it into a bedroom.

I thought landscaping mulch is bad as it contains fungicide and mostly bark. I'd have to look around town for somewhere that may be a dump for wood.

Heres some updated logs




Here is from a grain to grain of blue oysters.


Edited by vatman (07/16/15 10:28 AM)


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OfflineGr0wer
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Re: Oyster and Lions mane project/grow log [Re: vatman]
    #21951806 - 07/16/15 12:22 PM (8 years, 6 months ago)

Landscape mulch does work, the mulch that I use is just all the broken branches and trimmings the city collects. They shred it up and place it Into a dumpster for free pickup. Sure there are rocks and plastic mixed in but you can easily pick all that out. You can also try getting with a local tree removal service just be specific you want a healthy oak or hardwood tree. The only thing is they would dump a full load of 6-10 yards, if you have a garden you can use it as landscape mulch since you'll have so much.


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Invisiblevatman
I'm Vatman


Registered: 04/17/14
Posts: 1,642
Re: Oyster and Lions mane project/grow log [Re: Gr0wer]
    #21956595 - 07/17/15 03:01 PM (8 years, 6 months ago)

Options are rather limited local

Here is what I can buy locally in terms of wood products.




The bark from what I have read can only be used up to 20% of the product. I haven't seen logs on garden mulch bough from a store.

My other local options are to buy or barrow a wood chipper(been asking friends all ready. Most don't have them) and shred local branches. There are plenty of logs and branches around. Turning logs into indoor suitable substrate I would imagine being much more difficult.

Other option would be taking a 50 min one way trip and checking out a bunch of stores to see what hardwood options I would have. Down there I may be able to get some options of compost.


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OfflineGr0wer
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Re: Oyster and Lions mane project/grow log [Re: vatman]
    #21956713 - 07/17/15 03:34 PM (8 years, 6 months ago)

Try a local nursery, they might have bulk mulch. It needs to be hardwood mulch. Did you try your local town municipalities or local tree companies? You could even try calling a tree company and ask them if you can stop by at a job and steal a few pails of mulch.


Edited by Gr0wer (07/17/15 04:00 PM)


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Invisiblevatman
I'm Vatman


Registered: 04/17/14
Posts: 1,642
Re: Oyster and Lions mane project/grow log [Re: Gr0wer]
    #21956810 - 07/17/15 04:09 PM (8 years, 6 months ago)

Nusry only had bark and cedar. Cedar being an antifungal wood. Town hall said the only local place would be the dump (full on im the ground dump off a highway) the local tree company could be a good bet


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OfflineGr0wer
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Re: Oyster and Lions mane project/grow log [Re: vatman]
    #21956892 - 07/17/15 04:33 PM (8 years, 6 months ago)

Does the dump have a separate pile for mulch? Id stop by or call the dump and see.


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Invisiblevatman
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Posts: 1,642
Re: Oyster and Lions mane project/grow log [Re: Gr0wer]
    #21956939 - 07/17/15 04:48 PM (8 years, 6 months ago)

Got off the phone with the dump, they do have a separate place for wood. The rep on the phone said all they had were small branches and sagebrush. Ill try giving the local tree cutting company a call tomorrow, just about everything shuts down at 4 here..


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