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ericfloyd64
Stranger

Registered: 03/28/15
Posts: 99
Last seen: 8 years, 3 months
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Diluting a spore syringe to get more out of it
#21686703 - 05/16/15 11:07 AM (8 years, 8 months ago) |
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Hey guys, I bought a pan cambo "Goliath" spore syringe and instead of it being filled completely, the tap that has the name of the strain was covering the last 2 or 3 cc markers. And after I shot one jar I had about 6 or 7 cc's, I had only shot about one into the jar. Now I'm down to about .5 cc's and I was wondering if I could poor this solution into some distilled water and dilute it a tad just so I can get two jars out of it. I just felt a little ripped off and I want to make at least make 7 jars
Edited by ericfloyd64 (05/17/15 06:59 PM)
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Diluting a spore syringe to get more out of it [Re: ericfloyd64]
#21686717 - 05/16/15 11:13 AM (8 years, 8 months ago) |
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You should make some agar. You'd only need about a drop of spores to make unlimited jars.
I'm not entirely convinced you'll have it cleanly diluted, but if you did and you can see black specks (spores), you could easily dilute it further 10 fold.
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Sksoul
Pan handler



Registered: 10/31/14
Posts: 397
Loc: Far East
Last seen: 3 years, 6 months
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Re: Diluting a spore syringe to get more out of it [Re: ericfloyd64]
#21686719 - 05/16/15 11:13 AM (8 years, 8 months ago) |
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You could try agar and let that .5ml last you years. Diluting should be fine, but you have to make sure your water is clean. That means pcing it first.
Just try agar or buy another syringe.
-------------------- Like all great travellers, I have seen more than I remember, and remember more than I have seen.
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ericfloyd64
Stranger

Registered: 03/28/15
Posts: 99
Last seen: 8 years, 3 months
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Re: Diluting a spore syringe to get more out of it [Re: Sksoul]
#21686753 - 05/16/15 11:24 AM (8 years, 8 months ago) |
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I've been meaning to get into agar, how do you do it exactly and what exactly is it?
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wowimflabbergasted
supercalifragilistic



Registered: 07/16/12
Posts: 18,918
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Re: Diluting a spore syringe to get more out of it [Re: ericfloyd64]
#21686784 - 05/16/15 11:36 AM (8 years, 8 months ago) |
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FreeWorldOrder


Registered: 12/24/13
Posts: 2,002
Loc: Indiana, USA
Last seen: 8 days, 10 hours
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I would also put the last of it to several agar plates. (one drop in center of each plate)
Check out the link in my sig for Pasty's Plates, it is an easy and very good TEK for just starting out with agar...
-------------------- "They who can give up essential liberty, to obtain a little temporary safety, deserve neither liberty nor safety." - Benjamin Franklin Lets Grow Mushrooms Videos PastyWhyte's Easy Agar TEK Agar's Liquid Inoculant TEK
Edited by FreeWorldOrder (05/16/15 01:36 PM)
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blackout


Registered: 07/16/00
Posts: 5,266
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Re: Diluting a spore syringe to get more out of it [Re: FreeWorldOrder]
#21687138 - 05/16/15 01:45 PM (8 years, 8 months ago) |
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Rather than squirt it into water I would rather suck up sterile distilled water into the syringe. Be sure to flame the needle too.
I sucked up some water with malt extract into this "empty" syringe and got growth as there were some spores obviously still stuck inside. This went into a LC and then on to agar and is now growing.

more details in this thread. http://www.shroomery.org/forums/showflat.php/Number/20962227
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Diluting a spore syringe to get more out of it [Re: blackout]
#21687188 - 05/16/15 02:04 PM (8 years, 8 months ago) |
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--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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ericfloyd64
Stranger

Registered: 03/28/15
Posts: 99
Last seen: 8 years, 3 months
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Re: Diluting a spore syringe to get more out of it [Re: cronicr]
#21687978 - 05/16/15 07:01 PM (8 years, 8 months ago) |
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Thanks guys I will do that tomorrow!
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Diluting a spore syringe to get more out of it [Re: ericfloyd64]
#21687998 - 05/16/15 07:04 PM (8 years, 8 months ago) |
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 13 hours
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Re: Diluting a spore syringe to get more out of it [Re: ericfloyd64]
#21688117 - 05/16/15 07:39 PM (8 years, 8 months ago) |
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Hey man you have the vendors name in your post
About sucking water into a syringe, someone once explained to me that once you push the plunger down the inside walls of the barrel of the syringe are no longer sterile because they are exposed to air.
IE: you have 1CC left in a syringe, that 1CC will be sterile but everything behind the plunger has been exposed to air, so if you were to pull up X amount of CCs of sterile water the syringe is now at risk for contams.
--------------------
 Amateur Mycologists United AMU Q&A
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