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Invisiblemicro
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Re: YOU DO NOT NEED A LAB TO PRACTICE MYCOLOGY [Re: bodhisatta]
    #21626716 - 05/02/15 04:48 PM (9 years, 18 days ago)

Quote:

bodhisatta said:
Depends on the agar for me :shrug: its associated yes but I believe its a shitty old association. Tomentose growth on plates have been some of my best grows.

Spores germinate in one place if you put them in one place... Doesnt have much to do with it though




So it seems...

You should try to avoid that, it'll colonize quicker.


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Re: YOU DO NOT NEED A LAB TO PRACTICE MYCOLOGY [Re: micro]
    #21626732 - 05/02/15 04:54 PM (9 years, 18 days ago)

Tomentose growth usually turns rhizo on spawn anyway


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Re: YOU DO NOT NEED A LAB TO PRACTICE MYCOLOGY [Re: bodhisatta] * 1
    #21629834 - 05/03/15 12:08 PM (9 years, 17 days ago)

Rhizo/tementose means nothing. I've had pure cultures go back and forth depending on the concentration of food.

This method of using MS bags would work. But it wouldn't be 100% consistent. I want to be able to know what my yield will be.

There's also the variable of spores being dirty. I'd expect 10% contamination with this method. That's way higher than the 1% I allow.


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Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.

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Re: YOU DO NOT NEED A LAB TO PRACTICE MYCOLOGY [Re: 36fuckin5]
    #21630856 - 05/03/15 04:37 PM (9 years, 17 days ago)

:whathesaid:


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OfflineKizzle
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Re: YOU DO NOT NEED A LAB TO PRACTICE MYCOLOGY [Re: bodhisatta]
    #21631680 - 05/03/15 08:13 PM (9 years, 17 days ago)

But it does mean something. It's indicating a change. Now in a situation where the substrate isn't altered and you see it lose rhizomorphic growth what do you thinks causing it?


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Re: YOU DO NOT NEED A LAB TO PRACTICE MYCOLOGY [Re: Kizzle]
    #21631844 - 05/03/15 08:42 PM (9 years, 16 days ago)

Ypd plates usually de-rhizo for me the culture still works just as well


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Invisiblemicro
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Re: YOU DO NOT NEED A LAB TO PRACTICE MYCOLOGY [Re: bodhisatta]
    #21634497 - 05/04/15 12:11 PM (9 years, 16 days ago)

From my experience it seemed to have a lot to do with humidity.

That's bro-science though. I got nothing to back it up.


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Offlineinvitro

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Re: YOU DO NOT NEED A LAB TO PRACTICE MYCOLOGY [Re: micro]
    #21685576 - 05/16/15 01:05 AM (9 years, 4 days ago)

I'm a little late to the party but ....

Micro, what sort of sterilizer/pressure cooker are you working with?

In my mind, the Prestos and the AAs do suck (unsterile) air back into the pressure cooker as they cool.  If they did not, there would necessarily be a vacuum inside the pressure cooker and the lid would be near impossible to get off(I can explain why this is so if an explanation is needed).  Therefore leaving a vessel (for liquid media) lid slightly open (when pressure cooking) would create a vector for contamination.  The liquid media vessel and the pc would both be cooling and sucking in unsterile air.

I'm assuming that you are sterilizing a liquid in a vessel like this right?

http://i01.i.aliimg.com/wsphoto/v1/2048803161_1/Pharmaceutical-vials-20ml-clear-injection-glass-bottle-crystal-medical-container-0-7OZ-100pcs-per-pack-liquids.jpg

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OfflineJenn
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Re: YOU DO NOT NEED A LAB TO PRACTICE MYCOLOGY [Re: invitro]
    #21686307 - 05/16/15 08:57 AM (9 years, 4 days ago)

Let your PC cool in front of a flow hood or do as RR said and dowse a thin, clean cloth in 91% alcohol.  Wouldn't those precautions help detour contamination?


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Re: YOU DO NOT NEED A LAB TO PRACTICE MYCOLOGY [Re: Jenn]
    #21686422 - 05/16/15 09:44 AM (9 years, 4 days ago)

Just let the filters on your jars do the work. Problem solved.


--------------------
Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.

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Re: YOU DO NOT NEED A LAB TO PRACTICE MYCOLOGY [Re: 36fuckin5]
    #21686441 - 05/16/15 09:50 AM (9 years, 4 days ago)

wurd.


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Offlineinvitro

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Re: YOU DO NOT NEED A LAB TO PRACTICE MYCOLOGY [Re: 36fuckin5]
    #21686810 - 05/16/15 11:48 AM (9 years, 4 days ago)

Quote:

36fuckin5 said:
Just let the filters on your jars do the work. Problem solved.




Which is what I do.  I just mentioned what I mentioned because OP showed a pic of a vessel without a filter, and this idea of cracking the lid.

I appreciate the info though OP!!

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Offlineknomadic_niki
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Re: YOU DO NOT NEED A LAB TO PRACTICE MYCOLOGY [Re: invitro]
    #21687916 - 05/16/15 06:43 PM (9 years, 4 days ago)

yes, invitro, you are right


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Offlinedrake89
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Re: YOU DO NOT NEED A LAB TO PRACTICE MYCOLOGY [Re: 36fuckin5]
    #21689359 - 05/17/15 04:26 AM (9 years, 3 days ago)

Quote:

36fuckin5 said:
Just let the filters on your jars do the work. Problem solved.




if you're in a dirty  area that's unadvisable.  if you cool down in front of a hood then you don't need to spray the jars before opening them.


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Re: YOU DO NOT NEED A LAB TO PRACTICE MYCOLOGY [Re: drake89]
    #21690768 - 05/17/15 02:24 PM (9 years, 3 days ago)

If you're in that dirty of an area, clean it up.


--------------------
Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.

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Offlinedrake89
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Re: YOU DO NOT NEED A LAB TO PRACTICE MYCOLOGY [Re: 36fuckin5]
    #21691360 - 05/17/15 05:00 PM (9 years, 3 days ago)

Quote:

36fuckin5 said:
If you're in that dirty of an area, clean it up.





wanna come clean 1000sqft of grow rooms :wink: ?

I bathe in trich before I inoculate 100 bags every day, 5 days a week.  It's all I can do to keep 100sq ft of lab clean.


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Re: YOU DO NOT NEED A LAB TO PRACTICE MYCOLOGY [Re: drake89] * 1
    #21692888 - 05/17/15 11:33 PM (9 years, 2 days ago)

If I had 1000 sq ft of grow space it'd be impeccabpy clean. Most of growing mushrooms is cleaning and prep work anyway.


--------------------
Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.

Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
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Re: YOU DO NOT NEED A LAB TO PRACTICE MYCOLOGY [Re: 36fuckin5]
    #21693513 - 05/18/15 06:15 AM (9 years, 2 days ago)

Pretty much.


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Re: YOU DO NOT NEED A LAB TO PRACTICE MYCOLOGY [Re: 36fuckin5]
    #21693627 - 05/18/15 07:50 AM (9 years, 2 days ago)

Quote:

36fuckin5 said:
If I had 1000 sq ft of grow space it'd be impeccabpy clean. Most of growing mushrooms is cleaning and prep work anyway.



that's why i'm changing my business model from direct marketing to wholesale.  at this point I'll take a price cut if it means one 2hr delivery each week vs 10+hrs.  and growing one species vs 10.


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Invisiblemicro
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Re: YOU DO NOT NEED A LAB TO PRACTICE MYCOLOGY [Re: Jenn]
    #21695652 - 05/18/15 07:23 PM (9 years, 2 days ago)

Quote:

Jenn said:
Let your PC cool in front of a flow hood or do as RR said and dowse a thin, clean cloth in 91% alcohol.  Wouldn't those precautions help detour contamination?




No. Neither one of those will do anything.

edit: unless you mean clean the inoculation site, then yes that will help

Why s everyone so stuck on jars? Because they are reusable?


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Edited by micro (05/18/15 07:30 PM)

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