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Offlinekaaskop
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Registered: 03/31/15
Posts: 35
Last seen: 8 years, 3 months
My first mexicana A from scletoria
    #21482644 - 03/31/15 09:35 AM (8 years, 10 months ago)

Hey Guys & Galls,

My name is kaaskop. I recently bought some Mexicana A called philosopher stones, but due to circumstances I couldn't eat them.

I decided to make a grow out of them. Just to see how necessary it is to follow the tek. (come on guys, I have seen ML-I labs covered in shits, and nobody complained. It seemed natural to try at least to do it my 'own way')
Conclusion one: FOLLOW THE DAMN TEK!
Conclusion two: Rice seems to be a better media than grains.
Conclusion three: rubbing alcohol in the air prevents fungal growth, but leaves yeast unharmed.
Conclusion four: Boiling the substrates for 70 minutes at 225 degrees C (celsius) did not kill all yeast.
Conclusion five: perforating the lid needs to be done before you start growing.

Anyhow, I learned a lot and I will keep scrolling and reading along the way. I just like to get busy while I learn. So far I found two, maybe three contams. Two of them are clearly yeast. One of them is yet to be identified. I wont have problems with that I guess, I found some ID keys around here, so that will be done when I have the time.

I made some pics, and I would love your opinion on how these fungi are doing. The came from scletoria (am I saying that right?).

So the techinacal info:
- Tap water (drinkable) 250 ml / Jar (pH=8-8.5)
- 1 jar with a cup of rice mix, added sweet corn (no contams, lots of myc forming)
- 1 jar with a cup of cereal mixed w/ fruits and coffee grounds (contam, considered dead)
- 1 jar with a cup of grains (contaminated with yeast showed up yesterday, but almost completely overgrown with myc.)
- 1 jar with LC (honey based, 2 tablespoons w/ 300mL water)
- 1 myc growing box, storebought with the same grains as the others (dried and h2o2 cleaned scletoria went into that one)

All kept in the oven at 225C for 70 minutes. I don't have a PC or ricecooker. Cut me some slack ;-) I know I did it wrong.

All jars are kept in a homemade glass box. This is connected to the vent of my indoor garden (no, it has corn and some other crops, nothing illegal). This will keep the temp around 21-27 degrees C.

I will try to add some pics so you can judge them.

scletoria swimming in LC

The LC itself, looks contaminated. I dont know. I shook it a little.


Rice. 3 days after shaking

Rice, same jar. 3 days after shaking



These grains contain yeast contam. But they are still filling up nicely. I would like to see what happens if I do nothing.

Thanks for your humble opinions guys!


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Offlinekaaskop
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Registered: 03/31/15
Posts: 35
Last seen: 8 years, 3 months
Re: My first mexicana A from scletoria [Re: kaaskop]
    #21482705 - 03/31/15 10:05 AM (8 years, 10 months ago)

Did I tell ALL were inoculated with sclerotia? Well, now you know. Some of it got lost in the substrate, but the white  myc on the sides of the jars looks like the myc coming directly from the nugs.
Is this observation (slightly) correct, or am I just seeing a big contaminated bunch of jars?


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OfflinePsilicon
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Registered: 08/26/12
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Re: My first mexicana A from scletoria [Re: kaaskop]
    #21484661 - 03/31/15 06:37 PM (8 years, 10 months ago)

Those jars are definitely contaminated, if you inoculated with that LC.  That LC itself is contaminated.

Just so you know, putting something in a 225C doesn't count as boiling it at 225C.  The mechanics of heat transfer are kind of different there.


--------------------
Agar - what, why and how?  Everything a beginner needs to know.
Oat Prep Tek
Bored?  Please take one of my experiments off my hands.


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Offlinekaaskop
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Registered: 03/31/15
Posts: 35
Last seen: 8 years, 3 months
Re: My first mexicana A from scletoria [Re: Psilicon]
    #21486271 - 04/01/15 03:02 AM (8 years, 10 months ago)

You are right,
I see it personally as 'boiling'. I know I should have used steam under pressure, but right now I don't have the materials to do so. This oven-stuff was the best I could do, but water doesn't exceed 100°C without pressure. Thats why i call it boiling. The 225°C was just a good temp in my eyes to make it boil fast.

Thanks for your reply. I will make an agar plate of my LC just to be sure. I got a lot of residue after boiling the LC, but there seems to be myc forming directly from the sclerotia/scletoria. This drops straight after moving the jar. It could be bacteria, I am aware of that. But I am going to try anyway.

The rest of the jars are inoculated with truffles straight from the store bought sealed bag. So any contams in there are from myself, or the bad sterilisation methods.

I will keep you guys posted on how the contam spreads, if anyone is interested.


Edited by kaaskop (04/01/15 03:09 AM)


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Offlinekaaskop
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Registered: 03/31/15
Posts: 35
Last seen: 8 years, 3 months
Re: My first mexicana A from scletoria [Re: kaaskop]
    #21500020 - 04/04/15 08:00 AM (8 years, 10 months ago)

The rice jar is not contaminated as far as I can find out. It is filling up nicely with one type of fungus, growing directly from the sclerotia. This is a slow growing white fungus, and it looks a little fluffy. The 'fluff' doesnt exceed 3mm in length.



The grain jar does not contain cobweb, it seems to be the same fungus as the rice jar. I have another jar that does have the cobweb mold; now I know what it is, compared to the other jars, the cobweb grows at 500% faster rate.
However, the grain jar is still contaminated with yeast and a green mold. I will consider it dead as well.


For a first grow, ignoring all the rules and having bad hygiene, 1/5 jars is a pretty good succes.

A PC and some syringes are coming this way. Hopefully I can start a grow following the rules next week.


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Offlinekaaskop
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Registered: 03/31/15
Posts: 35
Last seen: 8 years, 3 months
Re: My first mexicana A from scletoria [Re: kaaskop]
    #21519780 - 04/08/15 02:54 PM (8 years, 10 months ago)

The 'dead' yeast jar which contained a lot of evaporated alcohol (i washed the filter with it, stupid) has revived. This alcohol prevented fungal growth for cobweb, p. Mexicana and other contams. However, after opening the jar one or twice, alcohol levels have dropped and only the sclerotium is showing signs of active life.

LC contam confirmed.
Rest of the jars (excl. The rice one) have been disposed.

A new set or jars is PC'd and inoculated with B+ and Amazon(ian?).
I used a bicycle tire repair set to make my self healing holes, I figured processed rubber is a less favorable material for fungi to grow on than a gasket sealent. If the lids are sanded down a little, there is no need for material on the inside of the lid anymore. Thus it is less of a contam hazard in my view. After the puncture, if the hole doesnt close, a little bit of tire glue can close it off. It seems to be PC proof as long as you let it cool before you touch it.
Aeration is provided with triple layered PFF-II filters. I know this AE isnt necessary but I wanted to see how well they work.

Anything else? Besides the rice jar being succesfully filled. I have nothing more to share.


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InvisiblebodhisattaMDiscordReddit
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Re: My first mexicana A from scletoria [Re: kaaskop]
    #21520038 - 04/08/15 04:10 PM (8 years, 10 months ago)

A lot of what you figured is dead wrong. I would start with a proven tek and follow that until some success under your belt that some of the guesses you make will then be educated ones.


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OfflineSockadin
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Re: My first mexicana A from scletoria [Re: bodhisatta]
    #21520432 - 04/08/15 05:44 PM (8 years, 10 months ago)

Can I first start by saying, Welcome Aboard!

Next thing I want to address is your question. Bodhisatta and Van der Grien already pointed out a couple of issues, but the only question you asked is how does it look? It doesn't look good. :shrug:

Last think I want to point out and I know this is going to make me sound like an A@#hole is that this forum is dedicated to people needing tip and advise on the proper cultivation methods of Mushrooms. We share knowledge and answer questions for people who are dedicated to learning how to do this and get great success. I admire your "do it my own way" mentality but unfortunately it means that this thread doesn't belong in this forum.

Please feel free to visit this forum with any questions you have about proper growing methods. Also please don't take offense to my request to move this thread, remember the entire KB takes time out of their day, everyday to help people looking for it.


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OfflineAlan RockefellerM
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Re: My first mexicana A from scletoria (moved) [Re: kaaskop]
    #21520437 - 04/08/15 05:46 PM (8 years, 10 months ago)

This thread was moved from The Noob Forum.

Reason:
Requested by OP


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Offlinekaaskop
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Registered: 03/31/15
Posts: 35
Last seen: 8 years, 3 months
Re: My first mexicana A from scletoria (moved) [Re: Alan Rockefeller]
    #21522738 - 04/09/15 04:38 AM (8 years, 10 months ago)

* Re: My first mexicana A from scletoria (moved)

No worries, I understand your concerns. A forum needs to be clean and in a neat order.

Right now I switched from a bad start to a proven tek (besides the syringe hole and aeration). As I have told; I like to stay busy while I learn more and more. Please don't take offense in me ignoring the rules; I work at labs and I know how important these rules are. However, I never worked with fungi. So I just want to see how it works, any flaws, bad teks, the wrong stuff, have been done hundreds of times and I know these are documented. However, I still like making mistakes for the sake of it. My goal is to learn from these, step by step, not to make buckets full of shrooms. I can just buy those under the counter, or stones by mail order.
I figured to document my mistakes, my bad teks and conclusions just for noobs like me to read why they shouldnt do stuff.
A lot of underlying theory I cannot explain, I hoped someone would.
'Those look contamed' or 'a lof of what you figured is dead wrong' is not going to help me or them with that.
I figured the noob part of the forum was the right place for that. Guess I was dead wrong again ;-)
Thanks for the move, and thanks for not deleting the entire thread!
I will continue here.


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Offlinekaaskop
Stranger
Registered: 03/31/15
Posts: 35
Last seen: 8 years, 3 months
Re: My first mexicana A from scletoria [Re: kaaskop]
    #21686370 - 05/16/15 09:26 AM (8 years, 9 months ago)

At full colonisation, I started waiting for pins. Then I read these are only formed with multispore cultures. I figured this might be a monoculture.
Anyhow, because I like to do things hands-on, I introduced a casing layer of coco and ground gypsum (5% gypsum on 95% coco). I also took the cap off the jar, and let it dry out. I dunked it, which I thought was stupid. But the jar sucked up around 200ml. Something must have been dry in there.

I kept the top layer moist, and some overlay occurred. I scraped some, I replaced some. I took out some top layer because of trichoderma.

Now, two months later. Or maybe a little less, I have been treating them as regular cakes in a jar (misting a few times, leaving the top layer exposed to fresh air).
Today I took the jar apart, and I fished out 31 grams of wet sclerotia. How about that? In a 250ml jar, I think that's a crazy amount. They haven't been fully formed yet. At least, not all of them. The biggest is around 4cm in diameter, and grew near the top layer. It weighs a whopping 11 grams.

I did a lot of wrong conclusions. But the result is astonishing! Mexicana type A colonises very slow on everything. The wetter it gets, the more rhizomorphic, but also the slower it becomes. But rice seems the way to go for me.

This guy is happy to conclude, that some things can be done even though the experts do not like those ways.

This post is for the purpose of keeping track (because of the date stamps). Not a battle, nor a 'prove you wrong' thing. I am very pleased with these results, and in my time here, I keep learning every day.


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