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leo_spaceman
Stranger
Registered: 12/17/14
Posts: 8
Last seen: 8 years, 1 month
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PF Tek: 2 weeks little growth
#21686284 - 05/16/15 08:47 AM (8 years, 8 months ago) |
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Hi all,
This is my second grow and I followed a PF tek guide I found here. It's been two weeks since I inoculated, and I'm seeing very slow growth. Only a couple of the ten jars have visible mycelium. The growth for those jars is just a small blotch about the size of a dime, and only located under one of the four inoculation sites.
So here are my primary concerns:
1. I ground up brown rice myself this time and perhaps I didn't grind it fine enough.
2. When I sterilized the needle with a flame, I killed the spores in the needle, and then I injected dead spores into some of the jars.
3. The jars are in my basement and they're growing slowly because it's colder there than in the rest of my house or outside.
4. They're not getting much light where they are in the basement.
Should I be concerned about any of the above?
Should I make any changes?
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: PF Tek: 2 weeks little growth [Re: leo_spaceman]
#21686314 - 05/16/15 08:59 AM (8 years, 8 months ago) |
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#2 the tek says how to deal with that right in the directions
#3 68-72f is perfect. Put them upstairs...
#4 light is good at all stages. Put jars on a shelf or something
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leo_spaceman
Stranger
Registered: 12/17/14
Posts: 8
Last seen: 8 years, 1 month
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Re: PF Tek: 2 weeks little growth [Re: bodhisatta]
#21686343 - 05/16/15 09:14 AM (8 years, 8 months ago) |
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Quote:
bodhisatta said: #2 the tek says how to deal with that right in the directions
Here is what the link in your signature says:
Quote:
Flame sterilize the needle and don't wipe it with alcohol
Alcohol sanitizes flame sterilizes. Don't move backwards
Use 0.25CC(aka mL) per hole or less Make sure to get the needle against the glass and below the verm barrier.
Here is what EvilMushroom666's guide says (linked to from your guide):
Quote:
2. Next spray your water or bleach solution into the box before sealing it. You should have two openings in the front for your hands (wearing gloves wipe with alc of course). Outside of your box shake your syringe to distribute the spores evenly and then flame sterilize your needle until it is RED hot. Next wash your gloves with some ISO alcohol and then enter the glove box with your needle in hand.
3. Place the needle into the hole in your substrate jar at an angle so the tip of the needle is up against the glass. Then GENTLY inject 1/4th of a CC into the jar. Remove the needle and repeat for the other 3 holes. You should use 1CC per jar, or 1/4th of a CC per hole. Set that jar aside in your box.
4. Remove your hands and the syringe from the box, flame sterilize your needle again and repeat as needed.You need to flame sterilize your needle between jars. One 12CC syringe should be able to make 12 1/2 pint BRF cakes. Do not place the foil back on your jars.
I've read both a few times, and I still don't know what you're referring to.
Quote:
bodhisatta said: #3 68-72f is perfect. Put them upstairs...
#4 light is good at all stages. Put jars on a shelf or something
Thanks for the advice; I'll move them upstairs.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: PF Tek: 2 weeks little growth [Re: leo_spaceman]
#21686386 - 05/16/15 09:31 AM (8 years, 8 months ago) |
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http://www.mushroomvideos.com
It says what to do with spores. Then you click the link. My signature isn't the tek its links to teks and supplemental info.
The brf tek says to heat the needle to red hot squirt out a few drops to cool the needle then inject
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jbaby007
Badass



Registered: 02/28/15
Posts: 1,026
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Re: PF Tek: 2 weeks little growth [Re: leo_spaceman]
#21686415 - 05/16/15 09:40 AM (8 years, 8 months ago) |
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Pics? Maybe your substrate is too wet. Did you buy sponsor spores? Did you allow your jars to cool after steaming/pcing them? I don't think you really need to worry about the questions you listed but there are many factors that could lead to you not having growth.
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