|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
PinPornProducer
Buy the ticket, take the ride



Registered: 08/23/14
Posts: 9,981
Loc: Rocky Point R.I
Last seen: 6 years, 24 days
|
Re: PF Tek - dispute of need for a glove/still air box [Re: mushpunx]
#21688113 - 05/16/15 07:38 PM (8 years, 8 months ago) |
|
|
Open air works well in a bathroom steamed up from the shower running straigh hot water, ain't no mold spores floating around in that shit
|
bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
|
Re: PF Tek - dispute of need for a glove/still air box [Re: PinPornProducer]
#21688116 - 05/16/15 07:38 PM (8 years, 8 months ago) |
|
|
make a huge frame from PVC pipe and wrap the whole thing with crystal clear plastic and make some big arm holes.
|
MonkeyJesusFresco
am i suspended in agar?



Registered: 10/09/12
Posts: 3,306
Loc: South East USA
Last seen: 8 hours, 14 minutes
|
Re: PF Tek - dispute of need for a glove/still air box [Re: bodhisatta]
#21688130 - 05/16/15 07:43 PM (8 years, 8 months ago) |
|
|
if you're of a smaller stature, you could build a LARGE pvc frame, wrap it in clear painters tarp/plastic, crawl in, spray the walls of your tent with a 1:10 bleach:water + a drop of dish soap mixture and then lather yourself up with olive oil or vaseline, and then set very still for about 10-15 mins and let the air settle, and then just, you know, "go to town", just like any other saturday nite except you'll be inoculating jars
|
PinPornProducer
Buy the ticket, take the ride



Registered: 08/23/14
Posts: 9,981
Loc: Rocky Point R.I
Last seen: 6 years, 24 days
|
Re: PF Tek - dispute of need for a glove/still air box [Re: MonkeyJesusFresco]
#21688137 - 05/16/15 07:45 PM (8 years, 8 months ago) |
|
|
Quote:
MonkeyJesusFresco said: if you're of a smaller stature, you could build a LARGE pvc frame, wrap it in clear painters tarp/plastic, crawl in, spray the walls of your tent with a 1:10 bleach:water + a drop of dish soap mixture and then lather yourself up with olive oil or vaseline, and then set very still for about 10-15 mins and let the air settle, and then just, you know, "go to town", just like any other saturday nite except you'll be inoculating jars 
|
bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
|
Re: PF Tek - dispute of need for a glove/still air box [Re: PinPornProducer]
#21688144 - 05/16/15 07:47 PM (8 years, 8 months ago) |
|
|
good luck breathing all that bleach
|
2shoes
The anti-agar



Registered: 04/07/11
Posts: 3,124
Loc: Not in a SAB
|
Re: PF Tek - dispute of need for a glove/still air box [Re: PinPornProducer]
#21688149 - 05/16/15 07:49 PM (8 years, 8 months ago) |
|
|
For my situation they are more of a pain compared to my amount of fails I've had... different strokes for different folks. I see how easy they are and I have one that I use when I NEED it. Now for PF cakes as the OP stated yes SAB are great and work but NOT a must.
I PC my PFs btw.
Breathing bleach
|
MonkeyJesusFresco
am i suspended in agar?



Registered: 10/09/12
Posts: 3,306
Loc: South East USA
Last seen: 8 hours, 14 minutes
|
Re: PF Tek - dispute of need for a glove/still air box [Re: bodhisatta]
#21688159 - 05/16/15 07:51 PM (8 years, 8 months ago) |
|
|
oh good point! should add:
wear a respirator
that may sound crazy, and it is, but it's all technically valid... ya know, if you move slowly and don't disturb the air
|
mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 13 hours
|
Re: PF Tek - dispute of need for a glove/still air box [Re: MonkeyJesusFresco]
#21688280 - 05/16/15 08:32 PM (8 years, 8 months ago) |
|
|
I like the plexi glass sneeze guard style SAB myself!
I need a resperator. A real one like for fumes... Im sick of breathing in a quart of ISO every time I work
--------------------
 Amateur Mycologists United AMU Q&A
|
bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
|
Re: PF Tek - dispute of need for a glove/still air box [Re: mushpunx]
#21688291 - 05/16/15 08:35 PM (8 years, 8 months ago) |
|
|
don't use so much iso...
|
2bittoker
Resident PMP Advocate


Registered: 03/09/13
Posts: 555
Last seen: 2 years, 3 months
|
Re: PF Tek - dispute of need for a glove/still air box [Re: Late2theparty]
#21688362 - 05/16/15 08:49 PM (8 years, 8 months ago) |
|
|
Quote:
Late2theparty said: Okay I've done a whole lot of reading, and there are a few things on this point I'd like to have cleared up. When I first began, I assumed the layer of tinfoil (the one covering the jar lids)stayed over the jar lid, to prevent contams. A little reading has told me that in fact the foil comes off, exposing those holes to the environment for gas exchange.
With those holes freely exposed to the environment allowing spores and such to fall on the top layer of the vermiculite barrier, it seems using the SAB or glove box is a somewhat unnecessary extra complicating step.
While a properly made verm barrier is very contam resistant, its not 100% effective. The idea of using a SAB is not to achieve sterility but to minimize the amount of airborne contams the jar is exposed to.
Quote:
I understand the needle should be flamed, as it enters through and underneath the verm barrier. But why the need for such sterile conditions otherwise? Assuming of course the needle is used IMMEDIATELY after flaming.
Again, minimization of the amount of spores the jar is exposed to prior to 100% colonization. Its also why its recommended that you don't pick up or move the jars during the first two weeks until the mycelium can get a foothold.
Quote:
On a side note, are there going to be any major consequences to using only two holes instead of the four? The holes are rather generous ~3mm and up to 5-6mm for the ones where the needle caused the foil to tear when innoculating, so I would imagine a great enough volume of gases can be exchanged...
The only consequence is slower colonization times since you have 2 inoculation points instead of 4. But I have used two before when Ive been short on spore solution.
-------------------- “I slept and dreamt that life was joy. I awoke and saw that life was service. I acted and behold, service was joy.” "Love does not claim possession, but gives freedom" ― Rabindranath Tagore Stuff for New Growers Where new growers should start: RogerRabbit's PF Tek video How it Should and Shouldn't Look My Simplified Bulk Growing My OJ Shroom Tek
|
bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
|
Re: PF Tek - dispute of need for a glove/still air box [Re: 2bittoker]
#21688381 - 05/16/15 08:55 PM (8 years, 8 months ago) |
|
|
one hole would be enough for gas exchange the reason for 4 holes is to ensure full colonization happens, you could get away with 2 but just use half as much in 4
|
FreeWorldOrder


Registered: 12/24/13
Posts: 2,002
Loc: Indiana, USA
Last seen: 8 days, 10 hours
|
Re: PF Tek - dispute of need for a glove/still air box [Re: 2shoes]
#21690168 - 05/17/15 11:10 AM (8 years, 8 months ago) |
|
|
I believe you...
That's why I also agreed it "can be" done.
It's just poor sterile procedure and not a good habit for beginners to develop.
Wasn't trying to bust anyones balls or say it can't work.
Just trying to give good advice.
Like I said, the practice of good sterile procedure is why the majority of growers use a SAB or flow hood. It drastically reduces the chances of introducing contams...
-------------------- "They who can give up essential liberty, to obtain a little temporary safety, deserve neither liberty nor safety." - Benjamin Franklin Lets Grow Mushrooms Videos PastyWhyte's Easy Agar TEK Agar's Liquid Inoculant TEK
Edited by FreeWorldOrder (05/17/15 11:30 AM)
|
MonkeyJesusFresco
am i suspended in agar?



Registered: 10/09/12
Posts: 3,306
Loc: South East USA
Last seen: 8 hours, 14 minutes
|
Re: PF Tek - dispute of need for a glove/still air box [Re: FreeWorldOrder]
#21691580 - 05/17/15 05:54 PM (8 years, 8 months ago) |
|
|
pssshhhhh, 4 SHiPs and a polyfil-filter, no verm; only way I make cakes
|
Beethoven1770
Psychonaut


Registered: 05/17/15
Posts: 96
Last seen: 7 years, 4 months
|
Re: PF Tek - dispute of need for a glove/still air box [Re: MonkeyJesusFresco]
#21691707 - 05/17/15 06:19 PM (8 years, 8 months ago) |
|
|
Will micro-pore tape withstand pressure cooker temps (up to 250 F)?
-------------------- …infinity...
|
Dr. Baker
Stranger


Registered: 05/11/15
Posts: 23
Last seen: 7 years, 10 months
|
Re: PF Tek - dispute of need for a glove/still air box [Re: Beethoven1770]
#21693330 - 05/18/15 03:03 AM (8 years, 8 months ago) |
|
|
Quote:
Beethoven1770 said: Will micro-pore tape withstand pressure cooker temps (up to 250 F)?
I put it on after sterilization because one time it melted when i PCed it.
|
Beethoven1770
Psychonaut


Registered: 05/17/15
Posts: 96
Last seen: 7 years, 4 months
|
Re: PF Tek - dispute of need for a glove/still air box [Re: Dr. Baker]
#21695006 - 05/18/15 03:53 PM (8 years, 8 months ago) |
|
|
Cool, thanks!
-------------------- …infinity...
|
2bittoker
Resident PMP Advocate


Registered: 03/09/13
Posts: 555
Last seen: 2 years, 3 months
|
Re: PF Tek - dispute of need for a glove/still air box [Re: Dr. Baker]
#21695580 - 05/18/15 07:05 PM (8 years, 8 months ago) |
|
|
Quote:
Dr. Baker said:
Quote:
Beethoven1770 said: Will micro-pore tape withstand pressure cooker temps (up to 250 F)?
I put it on after sterilization because one time it melted when i PCed it.
If you put it on after sterilization there is no point. The tape is water resistant and helps maintain your moisture content during PCing/Steam Bathing. It does not act as a filter after inoculation as there is a hole punched in it. Plenty of people skip the tape since if the foil is done correctly, it usually doesn't matter.
I use the tape personally and PC it and no issues.
-------------------- “I slept and dreamt that life was joy. I awoke and saw that life was service. I acted and behold, service was joy.” "Love does not claim possession, but gives freedom" ― Rabindranath Tagore Stuff for New Growers Where new growers should start: RogerRabbit's PF Tek video How it Should and Shouldn't Look My Simplified Bulk Growing My OJ Shroom Tek
|
bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
|
Re: PF Tek - dispute of need for a glove/still air box [Re: 2bittoker]
#21695612 - 05/18/15 07:14 PM (8 years, 8 months ago) |
|
|
the tape doesn't do shit. don't bother with it, it just sticks up on your lids. the moisture content stays the even with tight foil steam has to penetrate your jar anyway make them right and you'll be fine.
|
Beethoven1770
Psychonaut


Registered: 05/17/15
Posts: 96
Last seen: 7 years, 4 months
|
Re: PF Tek - dispute of need for a glove/still air box [Re: bodhisatta]
#21697927 - 05/19/15 10:41 AM (8 years, 8 months ago) |
|
|
I was curious because even though I put the aluminum foil over the lids as tightly as I could, it looked like some condensation seeped into the dry verm layer somehow. I think I'm going to try putting foil tape (used by electricians; withstands up to 600 F) over the holes the next time I use the PC.
-------------------- …infinity...
|
bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
|
Re: PF Tek - dispute of need for a glove/still air box [Re: Beethoven1770]
#21697946 - 05/19/15 10:46 AM (8 years, 8 months ago) |
|
|
You don't want the holes plugged just do it the right way
|
|