Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Original Sensible Seeds Bulk Cannabis Seeds   Left Coast Kratom Buy Kratom Extract   North Spore Bulk Substrate   Mushroom-Hut Substrate Bags   PhytoExtractum Buy Bali Kratom Powder   Kraken Kratom Red Vein Kratom

Jump to first unread post Pages: 1 | 2 | 3 | Next >  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
InvisibleLate2theparty
Rookie
Male User Gallery


Registered: 07/20/13
Posts: 58
Loc: Australia Flag
PF Tek - dispute of need for a glove/still air box
    #21685967 - 05/16/15 05:50 AM (8 years, 8 months ago)

Okay I've done a whole lot of reading, and there are a few things on this point I'd like to have cleared up. When I first began, I assumed the layer of tinfoil (the one covering the jar lids)stayed over the jar lid, to prevent contams. A little reading has told me that in fact the foil comes off, exposing those holes to the environment for gas exchange.

With those holes freely exposed to the environment allowing spores and such to fall on the top layer of the vermiculite barrier, it seems using the SAB or glove box is a somewhat unnecessary extra complicating step.

I understand the needle should be flamed, as it enters through and underneath the verm barrier. But why the need for such sterile conditions otherwise? Assuming of course the needle is used IMMEDIATELY after flaming.

On a side note, are there going to be any major consequences to using only two holes instead of the four? The holes are rather generous ~3mm and up to 5-6mm for the ones where the needle caused the foil to tear when innoculating, so I would imagine a great enough volume of gases can be exchanged...


Edited by Late2theparty (05/16/15 06:06 AM)


Extras: Filter Print Post Top
InvisibleLate2theparty
Rookie
Male User Gallery


Registered: 07/20/13
Posts: 58
Loc: Australia Flag
Re: PF Tek - why the need for a glove/still air box? [Re: Late2theparty]
    #21685986 - 05/16/15 06:04 AM (8 years, 8 months ago)

UPDATE:
Based on paranoia of contams, literal tinfoil hats are now in use. 'shading' the jars from anything falling in, and allowing air exchange


Extras: Filter Print Post Top
OfflineDr. Baker
Stranger


Registered: 05/11/15
Posts: 23
Last seen: 7 years, 10 months
Re: PF Tek - dispute of need for a glove/still air box [Re: Late2theparty]
    #21686156 - 05/16/15 07:54 AM (8 years, 8 months ago)

I've never used a still air box but with the problems I've been having lately I'm seriously considering it. I've always thought it was not necessary for PF jars but every little bit helps. Do you have any micropore tape? Put that over the holes, it should be more effective than your tin foil hats. Can't imagine why you'd only want 2 holes instead of 4. It will probably take longer to colonize with only 2 noc points. I use 1/8 inch (about 3mm holes) not sure if you'd be losing moisture through larger holes or not.


Extras: Filter Print Post Top
Invisible2shoes
The anti-agar
Male


Registered: 04/07/11
Posts: 3,124
Loc: Not in a SAB Flag
Re: PF Tek - dispute of need for a glove/still air box [Re: Dr. Baker]
    #21686198 - 05/16/15 08:09 AM (8 years, 8 months ago)

I don't use a SAB for PF and haven't come across any problems. I have 4 holes in my lids for FAE... pics please!!


Extras: Filter Print Post Top
OfflineFerather
Mycological
Male User Gallery


Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom Flag
Last seen: 1 year, 2 months
Re: PF Tek - dispute of need for a glove/still air box [Re: 2shoes]
    #21686264 - 05/16/15 08:39 AM (8 years, 8 months ago)

A SAB is used to create either a temporary or permanent barrier around your project.

Generally it is used to protect work when modifying, such as bulking, or transfers such as g2g.

PF tek uses foil barriers, easy for use without a SAB for beginners, PF tek is designed to be easy.
Foil is not a safe bet for expert use, its not as effective as a SAB.


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

DTS DCH Driver for Realtek [DTS:X] - Unlocked and reprogrammed.


Extras: Filter Print Post Top
Invisible2shoes
The anti-agar
Male


Registered: 04/07/11
Posts: 3,124
Loc: Not in a SAB Flag
Re: PF Tek - dispute of need for a glove/still air box [Re: Ferather]
    #21686320 - 05/16/15 09:02 AM (8 years, 8 months ago)

Foil should only be used during the PC cycle then removed for inoculation and thrown away... you want GE.


Extras: Filter Print Post Top
OfflineFerather
Mycological
Male User Gallery


Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom Flag
Last seen: 1 year, 2 months
Re: PF Tek - dispute of need for a glove/still air box [Re: 2shoes]
    #21686437 - 05/16/15 09:48 AM (8 years, 8 months ago)

If your only interested in covering your holes, place your jars in a large box, preferable plastic with lid, cardboard might work but it will block light.
Make sure the container holds at least 2.5 x more volume than per jar. 1 x 240ml will need a 600ml box that can breathe.

One of these will do a few jars.


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

DTS DCH Driver for Realtek [DTS:X] - Unlocked and reprogrammed.


Extras: Filter Print Post Top
InvisibleMad Season
hookers and blackjack
 User Gallery


Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: PF Tek - dispute of need for a glove/still air box [Re: 2shoes]
    #21686449 - 05/16/15 09:53 AM (8 years, 8 months ago)

It's not actually a GE problem. It'd have to be very close to airtight for there not to be enough GE. You don't want any water building up and dripping onto the verm layer. Getting wet defeats the purpose of a dry verm layer.

I think a small shelter is a great idea! Unfortunately spores don't just fall in the air. It'd have to be still for them to do that. They drift with the air currents and there's currents everywhere


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Extras: Filter Print Post Top
OfflineFerather
Mycological
Male User Gallery


Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom Flag
Last seen: 1 year, 2 months
Re: PF Tek - dispute of need for a glove/still air box [Re: Mad Season]
    #21686490 - 05/16/15 10:09 AM (8 years, 8 months ago)

True point, I've never done brf tek so makes sense.

I use those boxes for their lip system, GE and barrier, even if somehow a spore got in, it's unlikely to swim up into a pint cup of WBR with lid.


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

DTS DCH Driver for Realtek [DTS:X] - Unlocked and reprogrammed.


Extras: Filter Print Post Top
Offlineburgerguy
Stranger


Registered: 08/04/14
Posts: 56
Last seen: 7 years, 7 months
Re: PF Tek - dispute of need for a glove/still air box [Re: Ferather]
    #21686541 - 05/16/15 10:24 AM (8 years, 8 months ago)

I've only done brf up until this point. I leave the foil on after inoculation. I haven't had any problems yet with contamination or colonization.


--------------------


Extras: Filter Print Post Top
InvisibleMad Season
hookers and blackjack
 User Gallery


Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: PF Tek - dispute of need for a glove/still air box [Re: burgerguy]
    #21686568 - 05/16/15 10:29 AM (8 years, 8 months ago)

But the condensation under the foil could leak into the inoculation holes dripping on top of the dry verm layer ruining the integrity of it, and giving a spot for baddies to germinate. You don't even need a lid. A dry verm layer is the filter requirement and you don't want it getting wet. The lid with holes micropore taped is an added counter measure


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Extras: Filter Print Post Top
Invisiblewowimflabbergasted
supercalifragilistic
 User Gallery


Registered: 07/16/12
Posts: 18,918
Re: PF Tek - dispute of need for a glove/still air box [Re: Mad Season]
    #21686583 - 05/16/15 10:34 AM (8 years, 8 months ago)

Mad Season is right, the foil has nothing to do with GE. I wish people would stop spreading this.


--------------------
TempMail | TempPMs
My Guide | MJ Grows



Extras: Filter Print Post Top
OfflineFreeWorldOrder
Male User Gallery

Registered: 12/24/13
Posts: 2,002
Loc: Indiana, USA Flag
Last seen: 8 days, 7 hours
Re: PF Tek - dispute of need for a glove/still air box [Re: wowimflabbergasted]
    #21687081 - 05/16/15 01:28 PM (8 years, 8 months ago)

I hear ya... there is A LOT of piss poor, if not downright false info on this thread.

There are reasons we practice good sterile procedure in this hobby. It is a good habit to develop.

I would NEVER recommend for anyone to not use a SAB for inoculations, grain to grain transfers, or agar work. Even when doing the PF-Tek. Especially bad advice for beginners.

I'm not saying that it can't be done, but is more luck than anything. Inoculating jars in the open air is just asking for problems....lol.

The only thing I do in "open air" is spawning fully colonized grains to properly prepared bulk substrates.


--------------------
"They who can give up essential liberty, to obtain a little temporary safety, deserve neither liberty nor safety." - Benjamin Franklin

Lets Grow Mushrooms Videos
PastyWhyte's Easy Agar TEK
Agar's Liquid Inoculant TEK


Edited by FreeWorldOrder (05/16/15 01:33 PM)


Extras: Filter Print Post Top
Invisible2shoes
The anti-agar
Male


Registered: 04/07/11
Posts: 3,124
Loc: Not in a SAB Flag
Re: PF Tek - dispute of need for a glove/still air box [Re: FreeWorldOrder]
    #21687809 - 05/16/15 06:06 PM (8 years, 8 months ago)

Quote:

wowimflabbergasted said:
Mad Season is right, the foil has nothing to do with GE. I wish people would stop spreading this.




Okay so the bad info I offered has comes the same conclusion... no foil!!


Quote:

FreeWorldOrder said:
I hear ya... there is A LOT of piss poor, if not downright false info on this thread.

There are reasons we practice good sterile procedure in this hobby. It is a good habit to develop.

I would NEVER recommend for anyone to not use a SAB for inoculations, grain to grain transfers, or agar work. Even when doing the PF-Tek. Especially bad advice for beginners.

I'm not saying that it can't be done, but is more luck than anything. Inoculating jars in the open air is just asking for problems....lol.

The only thing I do in "open air" is spawning fully colonized grains to properly prepared bulk substrates.




I do all of my inoculating at my dinner table without SAB and must have the best luck on earth because sometimes I leave my fan on too... :thisisheavyman:

I hate working out of a SAB and only do so for agar and G2G...

I can show you the jars too!!!
:goodluckwiththat2:


Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,889
Loc: Milky way
Trusted Cultivator
Re: PF Tek - dispute of need for a glove/still air box [Re: 2shoes]
    #21687944 - 05/16/15 06:50 PM (8 years, 8 months ago)

lots of people drive for years with no seat belt and never crash but when they do.


Extras: Filter Print Post Top
Invisible2shoes
The anti-agar
Male


Registered: 04/07/11
Posts: 3,124
Loc: Not in a SAB Flag
Re: PF Tek - dispute of need for a glove/still air box [Re: bodhisatta]
    #21687959 - 05/16/15 06:55 PM (8 years, 8 months ago)

I would feel comfortable inoculating a PF jar in a porta-potty or in the middle of the wood... its PF :shrug: I'm willing to gamble the $.50 in materials.


Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,889
Loc: Milky way
Trusted Cultivator
Re: PF Tek - dispute of need for a glove/still air box [Re: 2shoes]
    #21687980 - 05/16/15 07:01 PM (8 years, 8 months ago)

you can open air inoculate anything with a injection port be it a brf cake with the verm barrier or a rubber thing or a silicone SHIP. but I would just pony up and do it in a SAB or FH every time anyway. needless to say I don't use injection ports at all for anything anymore so I have to use a SAB.


Extras: Filter Print Post Top
Offlinemushpunx
Fungus Punk
 User Gallery


Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 10 hours
Trusted Cultivator
Re: PF Tek - dispute of need for a glove/still air box [Re: bodhisatta]
    #21688096 - 05/16/15 07:31 PM (8 years, 8 months ago)

Quote:

bodhisatta said:
you can open air inoculate anything with a injection port be it a brf cake with the verm barrier or a rubber thing or a silicone SHIP. but I would just pony up and do it in a SAB or FH every time anyway. needless to say I don't use injection ports at all for anything anymore so I have to use a SAB.





:whathesaid:

I agree, you *can* get away doing PF tek open air. But an SAB is so cheap to construct, so easy to utilize.. why wouldnt you use it :shrug:


--------------------

Amateur Mycologists United
AMU Q&A


Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,889
Loc: Milky way
Trusted Cultivator
Re: PF Tek - dispute of need for a glove/still air box [Re: mushpunx]
    #21688105 - 05/16/15 07:35 PM (8 years, 8 months ago)

people think they have to build a shitty SAB and deal with working in that most people are too ignorant to realize you could make a 5 sided box and leave the whole front off and that would work way better than open air. you can make a SAB out of more than just totes if you need more arm room or shoulder width too.


Extras: Filter Print Post Top
OfflineMonkeyJesusFresco
am i suspended in agar?
Male


Registered: 10/09/12
Posts: 3,306
Loc: South East USA Flag
Last seen: 5 hours, 46 minutes
Re: PF Tek - dispute of need for a glove/still air box [Re: mushpunx]
    #21688109 - 05/16/15 07:37 PM (8 years, 8 months ago)

just a big tub turned over and sticking over the edge of the table, just enough room to reach your arms in; cheap.

shit, I've done it with a cardboard box sprayed with bleach-water w/ arm holes cut into the sides and saran wrap over the top (so I could see in), terrible idea btw, but it worked


Extras: Filter Print Post Top
Jump to top Pages: 1 | 2 | 3 | Next >  [ show all ]

Shop: Original Sensible Seeds Bulk Cannabis Seeds   Left Coast Kratom Buy Kratom Extract   North Spore Bulk Substrate   Mushroom-Hut Substrate Bags   PhytoExtractum Buy Bali Kratom Powder   Kraken Kratom Red Vein Kratom


Similar ThreadsPosterViewsRepliesLast post
* Pf tek newbie Q Natural2me 3,954 13 03/13/17 08:30 PM
by Shrewmdoom
* Glove Box Question The_Mushroom_God 4,318 6 04/30/02 10:51 PM
by bluepoo
* Why is the PF tek recommended for newbies? GaryW 9,932 8 07/27/02 07:08 AM
by SwondPooping
* Noooooo I am a stupid person or something.. contam Splat 2,768 9 03/07/02 06:55 AM
by Seuss
* Glove Box foo 1,037 7 05/27/05 03:58 PM
by yumyum
* Post deleted by users_request malibu76 1,047 2 06/29/02 03:48 AM
by resin
* glove box designs stan 2,587 2 05/01/02 05:52 PM
by Orchidman
* glove box or oven tek? mellojello 5,954 15 04/07/19 08:12 AM
by bodhisatta

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
3,567 topic views. 34 members, 240 guests and 37 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.038 seconds spending 0.008 seconds on 14 queries.