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Invisibleoontribe
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mycelium stopped brf cake
    #21685857 - 05/16/15 03:50 AM (8 years, 8 months ago)

so i inculated 5 jars my sterilization was up to par (also the pot got dried for a few minutes and i had to add more water)...and all the jars were growing fast so in two weeks:

2 jars are 100% and now they are in the one week consolidation period.

3rd jar is 95% and stopped! the mycelium isn't growing like a web again on the uncolonized part but making an edge...and there is a light white color growing on the corner of the uncolonized substrate. (like the color you get on the first few days on the points of inoculation)

4th jar is 80% and now its growing slowly! or maybe it stalled!

5th jar is like 99%!!! and stopped on this tiny substrate part!

the thing is i am using cups covered with foil as lids so i am not confident bout turning them upside down...i have made the holes bigger on the stalled jars!
no sign of contamination.
they are stored in an closed rack...should i leave it opened?

any advice?
gonna post pictures soon...am at work!


Edited by oontribe (05/16/15 05:10 AM)


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Offlinekn33b
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Re: mycelium stopped brf cake [Re: oontribe]
    #21686126 - 05/16/15 07:40 AM (8 years, 8 months ago)

Okay, a couple questions:
How long ago did you knock these up?
Are you using a verm barrier on top like PF tek?
Have you been moving them excessively?
Any yellowish tint on the perimeter of myc colonizing?

It's normal for MS jars to colonize at different rates, btw.


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Invisibleoontribe
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Re: mycelium stopped brf cake [Re: kn33b]
    #21686132 - 05/16/15 07:45 AM (8 years, 8 months ago)

knock them up as inoculated em? 2 weeks and a day.
yeap i am using verm barrier
nope i checked them 3 times since inoculation
no yellowish tint.

and yea i know they usually colonize at a different rate...and i used spore print. mexi-cub.


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Offlinekn33b
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Re: mycelium stopped brf cake [Re: oontribe]
    #21686169 - 05/16/15 07:58 AM (8 years, 8 months ago)

And if you're using cups covered with foil, and it's got a couple holes in it, you should be good. Patience is key, especially with MS crapshots. What you're describing has happened to all of my jars.


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Invisible2shoes
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Re: mycelium stopped brf cake [Re: kn33b]
    #21686211 - 05/16/15 08:15 AM (8 years, 8 months ago)

Post pic please... what size jars? 2 weeks seems fast for spore germination and full colonization.


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Invisibleoontribe
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Re: mycelium stopped brf cake [Re: 2shoes]
    #21686319 - 05/16/15 09:02 AM (8 years, 8 months ago)

2 jars 270 ml 3 others are 200ml
1 270 and 1 of the 200 are fully colonized now!
gonna post pics.


Edited by oontribe (05/16/15 09:02 AM)


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Invisibleoontribe
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Re: mycelium stopped brf cake [Re: oontribe]
    #21686808 - 05/16/15 11:48 AM (8 years, 8 months ago)






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InvisibleMad Season
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Re: mycelium stopped brf cake [Re: oontribe]
    #21686815 - 05/16/15 11:50 AM (8 years, 8 months ago)

I'm not convinced they're stalled. Betcha they're just growing slowly. They do have a larger surface area to do in the last stages of colonization. Just be patient. Give them a week or 2.


Edited by Mad Season (05/16/15 11:51 AM)


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Offlinekn33b
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Re: mycelium stopped brf cake [Re: Mad Season]
    #21686925 - 05/16/15 12:29 PM (8 years, 8 months ago)

:whathesaid:
2 weeks is usually when I start to see like a half dollar sized colony. Either way, they look healthy and you're ahead of the race.


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OfflineFreeWorldOrder
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Re: mycelium stopped brf cake [Re: kn33b]
    #21687120 - 05/16/15 01:41 PM (8 years, 8 months ago)

Don't flip them upside down... :thumbdown:


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Invisibleoontribe
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Re: mycelium stopped brf cake [Re: FreeWorldOrder]
    #21687205 - 05/16/15 02:10 PM (8 years, 8 months ago)

I have already enlarged the holes in the tin foil lid to the size a pen before posting this thread...hope that wouldn't affect the result!
patience it is!


Edited by oontribe (05/16/15 02:52 PM)


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Invisibleoontribe
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Re: mycelium stopped brf cake [Re: oontribe]
    #21721335 - 05/25/15 03:14 PM (8 years, 8 months ago)

Nearly ten days and no sign of improvement!

The metabolite spot got bigger or it could be contamination!!
And now there is a small pin.

Should i toss it or wait or what?




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InvisibleLocN9ne
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Re: mycelium stopped brf cake [Re: oontribe]
    #21721492 - 05/25/15 03:53 PM (8 years, 8 months ago)

Birth it, and remove any uncolonized parts...dunk it, roll it, fruit it... that is the route I would take if it were me...I had some cakes do something like that.


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Offlinekn33b
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Re: mycelium stopped brf cake [Re: oontribe]
    #21721693 - 05/25/15 04:46 PM (8 years, 8 months ago)

Quote:

oontribe said:
Nearly ten days and no sign of improvement!

The metabolite spot got bigger or it could be contamination!!
And now there is a small pin.

Should i toss it or wait or what?






My bet is bacteria. Open the jar, birth it, and give it a whiff at the problem area. It should smell like rotten apples at that point. With gloves on, cut out the uncolonized grain and cut back the myc (around the problem area) about a quarter inch. Soak the whole thing in a 1:10 peroxide and water bath. Wait a couple minutes and repeat until the peroxide stops foaming up. Rinse in spring water then crumble the cake up and mix myc crumbles 1:1 with hpoo/verm or coir/verm in a pan. Let colonize then add a casing layer and voila!



I've had amazing luck saving colonies using this method. For some reason, the peroxide makes it explode with growth overnight. Good luck!


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Invisibleoontribe
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Re: mycelium stopped brf cake [Re: kn33b]
    #21723398 - 05/26/15 02:13 AM (8 years, 8 months ago)

ok so i birthed it!
the uncolonized part was unattached to the rest of the cake...just like the dry verm on the top...so i washed it and dunked it.
the myc...looks kinda weak its not all white i can see the verm in some parts.
and no bad smell in the cup or the cake.
smells like mushroom to me...maybe ill post picture later.


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