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OfflineMysticPop
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Registered: 05/05/15
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Need Help with Agar Tek . Please help!!
    #21685613 - 05/16/15 01:25 AM (8 years, 8 months ago)

Hi

Trying to inoculate agar prepared using PatsyWhyte Agar Tek with spore syringes. got agar done using patsywhites receipe. Instead of waiting overnight,cooled the mini glads in fridge. Now i have some condensation in the inside. So here are my queries:-

1. Is it ok to inoculate while there is some condensing water sticking to the wall of the mini glad round containers?

2. Is it advisable to use spore prints or spore syringes received from a store?

3. Any other suggestions? :laugh:

Cheers
MysticPop


Edited by MysticPop (05/16/15 01:39 AM)


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InvisibleMad Season
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Re: Need Help with Agar Tek . Please help!! [Re: MysticPop]
    #21685658 - 05/16/15 01:45 AM (8 years, 8 months ago)

1. Yes
2. Either are brilliant. If print might want to look into an inoculation loop. If you're lazy hit up a swab
3. Cool them in the still air box. It doesn't even take long to cool. Agar hardens quickly. Less than an hour really.


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OfflineMysticPop
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Re: Need Help with Agar Tek . Please help!! [Re: Mad Season]
    #21685710 - 05/16/15 02:12 AM (8 years, 8 months ago)

Also after Inoculation, how do i store the mini glad rounds?


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Invisiblestareatclouds
star eat clouds?
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Re: Need Help with Agar Tek . Please help!! [Re: MysticPop]
    #21685834 - 05/16/15 03:33 AM (8 years, 8 months ago)

You put the lid back on within your SAB and then set them out wherever. I leave mine on top of my fridge in a box (not closed).

I also wipe the condensation off with the paper towel I have stuffed in the top in my SAB. It's not mandatory, but I like to be able to see the growth better.


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OfflineMysticPop
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Re: Need Help with Agar Tek . Please help!! [Re: stareatclouds]
    #21687104 - 05/16/15 01:36 PM (8 years, 8 months ago)

Thanks for everyone's reply.

How long does it take to see any growth on these agar glad mini rounds?


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InvisibleMad Season
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Re: Need Help with Agar Tek . Please help!! [Re: MysticPop]
    #21687112 - 05/16/15 01:38 PM (8 years, 8 months ago)

A couple days to a few weeks sometimes more. Literally that much of a time frame. Just be patient and don't move them around too much


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Edited by Mad Season (05/16/15 01:39 PM)


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