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OfflineJsneeb
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Registered: 04/05/15
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Last seen: 6 years, 10 months
Spawning to Bulk BRF jar which has stalled. Remove BRF or spawn everything?
    #21684315 - 05/15/15 06:48 PM (8 years, 8 months ago)

So I have two 1/2 pint BRF jars and a pint jar which I would like to spawn into my newly built monotub. 2 pints total.

Hoever for the past 2+ weeks the jars have made no more progress towards colonization. It has been exactly one month from inocculation and they took off fast.

There is no evidence of contamination, But the bottom of the jar had heavily packed material and I think the mycelium just cant make it though there.

Here is my Plan.

Birth the cake into a clean ziplock bag. Crumble the cake, and mix thoroughly with the uncolonized BRF.
Mix with my 50/50 coir verm which will be pasturized according the TEK.
I could also sterilize my 50/50 in the PC. Would this be wise?

Am I guaranteed to get contamination due to the non fully colonized BRF attracting contams?

My other idea was to birth the cake and wash away the BRF under steady running water and take just the Mycelium into the bag and crumble.

I plan to mix spawn to bulk 1:1 ratio. and case with 1/2 inch of coir/verm So I will have 4 pints total which will be placed into a large Tupperware container and closed with the lid to allow colonization of the substrate.

This container will then be placed in my small monotub and any excess space around it will be taken up with damp perlite to aid in moisture.

As you can tell newb here. Any suggestions or is this bound to fail? I have a small monotub already built but even so I do not have enough spawn to fully fill the bottom deeper than one inch ( yet ). Once my agar supplies show up I will get into grains and proper bulk subs.


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Titled, Mushrooms, Thirsty Water Bottle, And Boiling Peanut-butter?

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OfflineJsneeb
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Registered: 04/05/15
Posts: 109
Last seen: 6 years, 10 months
Re: Spawning to Bulk BRF jar which has stalled. Remove BRF or spawn everything? [Re: Jsneeb]
    #21684991 - 05/15/15 10:10 PM (8 years, 8 months ago)

No responses hu?

I did a few searches and it seems leaving the un-colonized BRF in the mix will grow alot of contams.

I will go ahead and wash the cakes down fully and remove the uncolonized BRF from the cake.

I hope washing it thoroughly and leaving only the mycelium will be enough to avoid contamination.

My bulk sub was cooked up 2 hours ago and is now cool.

Ready to go.

Please respond if you read this though I'll check back in a half hour right before I birth the 85-90% colonized cakes.  to see if anything new has come up.


--------------------
Read my trip report :smile:

Titled, Mushrooms, Thirsty Water Bottle, And Boiling Peanut-butter?

http://www.reddit.com/r/shrooms/comments/37z9rp/mushrooms_thirsty_water_bottle_and_boiling/


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OfflineMacaman
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Registered: 04/22/15
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Last seen: 1 year, 8 months
Re: Spawning to Bulk BRF jar which has stalled. Remove BRF or spawn everything? [Re: Jsneeb]
    #21685559 - 05/16/15 12:57 AM (8 years, 8 months ago)

I would wash off the un colonized BRF. And crumble the good stuff. I think that's your best bet.

Be rdy for the bulk grow to take a cpl weeks to re colonize before trying to put it into a fruiting stage.

Patience will be key for this grow.

Good luck and post the results. :cool:


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OfflineJsneeb
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Registered: 04/05/15
Posts: 109
Last seen: 6 years, 10 months
Re: Spawning to Bulk BRF jar which has stalled. Remove BRF or spawn everything? [Re: Macaman]
    #21685899 - 05/16/15 04:39 AM (8 years, 8 months ago)

That's exactly what I did.
I've got a bad feeling about this one though. Maybe if someone can help me find some pictures of crumbled BRF cakes that would help but mine looked almost like the mycelium was living only on the outside of the substrate and hadn't propagated inwards at all.

When I removed an crumbled the cake it was very dense and not white at all on the inside. It also felt dry.

Perhaps my moisture content was off and the Mycelium never made it though?

Anyway, I'll let it consolidate for 2 weeks and see how it is. Contams are expected .


--------------------
Read my trip report :smile:

Titled, Mushrooms, Thirsty Water Bottle, And Boiling Peanut-butter?

http://www.reddit.com/r/shrooms/comments/37z9rp/mushrooms_thirsty_water_bottle_and_boiling/


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