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OfflineYaMoonSun
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Agar question
    #21680299 - 05/14/15 07:20 PM (8 years, 8 months ago)

If I decide to get into agar, and I take a tissue sample from a specimen I really love, and it has multiple sectors, are each one of those a phenotype? Do I need to grow out each sector and see if it's worth pursuing? I've heard of people taking 10's of petries trying to get a single sector isolate, and I don't want to essentially wind up with 40 single sector isolates and not know what phenotype I have. Seems like a lot of time consumption. Got any grow pics from your single sector isolates?


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InvisiblebodhisattaMDiscordReddit
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Re: Agar question [Re: YaMoonSun]
    #21680435 - 05/14/15 07:52 PM (8 years, 8 months ago)

a phenotype you should look up what that means.

how it looks on agar could be a phenotype

each one of those sectors may be a strain. your clone is muli-strain and I would just use it as is. you don't have to isolate down from a clone if it's multi-nucleate multi-genetic multi-strain etc however you want to call it for this fungus. most clones work best as is. there's endless phenotypes. that's how it appears to our minds based on what's inside of it's genes.


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InvisibleGrey
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Re: Agar question [Re: YaMoonSun]
    #21680447 - 05/14/15 07:53 PM (8 years, 8 months ago)

You don't have to isolate that far if you don't want to. A clone can still give you good results.

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Edited by Grey (05/14/15 07:54 PM)


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InvisiblePirateSwazey
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Re: Agar question [Re: bodhisatta]
    #21680935 - 05/14/15 09:54 PM (8 years, 8 months ago)

Quote:

bodhisatta said:
most clones work best as is.



:imwithstupid:


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OfflineYaMoonSun
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Re: Agar question [Re: PirateSwazey]
    #21681234 - 05/14/15 11:15 PM (8 years, 8 months ago)

I took an LC, and it worked quite fast, but I had to dispose of everything before I saw the results. It was taken from a hydra/mutant, so it would have been a really crazy looking grow. Am going to see what I get from the spore-print I was donated though. Hoping some neat variables.


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OfflineYaMoonSun
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Re: Agar question [Re: bodhisatta]
    #21681241 - 05/14/15 11:18 PM (8 years, 8 months ago)

Quote:

bodhisatta said:
a phenotype you should look up what that means.

how it looks on agar could be a phenotype

each one of those sectors may be a strain. your clone is muli-strain and I would just use it as is. you don't have to isolate down from a clone if it's multi-nucleate multi-genetic multi-strain etc however you want to call it for this fungus. most clones work best as is. there's endless phenotypes. that's how it appears to our minds based on what's inside of it's genes.



For example, I picked a really large mushroom on my first flush, and a mushroom that was also very large grew directly from the spot that I had picked it from. (At little piece of mushroom that held on) Though it wasn't quite as pretty. It was big and potent, but not as dense. The first mushroom was like the densest mushroom I've ever seen/eaten.


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