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OfflineKingcannon
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Hydrogen Peroxide use...
    #21678470 - 05/14/15 10:42 AM (8 years, 8 months ago)

Hi , I cant just let contamination grows go in the bin so Im always attempting to find ways to bring them back to life ( especially after waiting for them to cover). I got a slime mould contamination that was eating its way through several of my tubs one especially badly, I washed them all off several times and let them soak in fresh water.
One with a deep valley into it I watched as it grew orange headed slime mould, I removed it then treated the whole area with 30% Vols Hydrogen Peroxide and the mycelium grew back and is even sprouting mushroom heads. I also treat the perlite to several large drops of peroxide to oxygenate and seems to work well.

http://s1346.photobucket.com/user/kingcannon1/B%20plus%20Second%20Attempt/B%20Plus%203rd%20attempt/story


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Offlineel8tch
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Re: Hydrogen Peroxide use... [Re: Kingcannon]
    #21678498 - 05/14/15 10:57 AM (8 years, 8 months ago)

bury them outside..


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InvisibleMad Season
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Re: Hydrogen Peroxide use... [Re: el8tch]
    #21678763 - 05/14/15 12:30 PM (8 years, 8 months ago)

Quote:

el8tch said:
bury them outside..



:whathesaid: seriously dude don't fuck with contaminations. Especially ones you don't know what they are. There's LOTS of molds out there that could throw you in the hospital for weeks if not months, or worse you could die too..


--------------------
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Edited by Mad Season (05/14/15 12:30 PM)


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InvisibleTheEaglesGift
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Re: Hydrogen Peroxide use... [Re: Mad Season] * 1
    #21678825 - 05/14/15 12:51 PM (8 years, 8 months ago)

Stop learning how to fight contaminants, and start learning how to prevent them. :thumbup:


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OfflineKizzle
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Re: Hydrogen Peroxide use... [Re: TheEaglesGift] * 1
    #21678938 - 05/14/15 01:33 PM (8 years, 8 months ago)

No one is going to die simply because their substrate got contaminated. Not trying to encourage people to keep their contaminated substrates around or anything but no need for them to shit bricks either every time they run into a contaminant either.

If you can't bare to toss it at least move it outdoors.


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InvisibleUncleFester
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Re: Hydrogen Peroxide use... [Re: TheEaglesGift]
    #21678977 - 05/14/15 01:42 PM (8 years, 8 months ago)

Quote:

TheEaglesGift said:
Stop learning how to fight contaminants, and start learning how to prevent them. :thumbup:



Aka Better Git Gud


--------------------
:pm:Looking for Penis Envy & Psilocybe Cubensis Cuba spores.:pm:


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InvisibleMad Season
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Re: Hydrogen Peroxide use... [Re: Kizzle]
    #21679138 - 05/14/15 02:39 PM (8 years, 8 months ago)

Quote:

Kizzle said:
No one is going to die simply because their substrate got contaminated. Not trying to encourage people to keep their contaminated substrates around or anything but no need for them to shit bricks either every time they run into a contaminant either.

If you can't bare to toss it at least move it outdoors.



Although I completely agree, I still think putting every contaminated substrate into the bath tub, sink, etc. and trying to dissect/flush the contams out will eventually result in a hospital visit one day before long.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

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Edited by Mad Season (05/14/15 02:54 PM)


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OfflineDetectiveLefty
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Re: Hydrogen Peroxide use... [Re: Mad Season]
    #21679159 - 05/14/15 02:47 PM (8 years, 8 months ago)

I've gotten trich so bad so often that I isolate & autoclave/PC *any* contaminated substrate and then compost it outside. Better safe than sorry... don't want spores all over my skin & clothes.

If it's a unique strain you want to save, maybe isolate on agar, otherwise get it out of your grow space & start over. Much less work in the long run.


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I swear on a stack of Bibles that everything I said in the above post is the 100% literal Truth.


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InvisibleUncleFester
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Re: Hydrogen Peroxide use... [Re: DetectiveLefty]
    #21682190 - 05/15/15 08:53 AM (8 years, 8 months ago)

Hmm I'd try to be more sterile but isolating the PC?  Lmao wut? :whatwhatwhat:


--------------------
:pm:Looking for Penis Envy & Psilocybe Cubensis Cuba spores.:pm:


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InvisibleMad Season
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Re: Hydrogen Peroxide use... [Re: UncleFester]
    #21682199 - 05/15/15 08:58 AM (8 years, 8 months ago)

Haha you misread what he said. He isolates contaminated stuff and puts them in the pc to kill the contamination before emptying the jars. That way there's no spores to go all over him and it'd keep the space a lot cleaner


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
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InvisibleUncleFester
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Re: Hydrogen Peroxide use... [Re: Mad Season]
    #21683169 - 05/15/15 01:34 PM (8 years, 8 months ago)

Oh lmao dude what a misunderstanding! And yeah that makes sense, I've pastureized a contaminated pf tek jar in a pot of boiling water before with good results on the disposal. But yeah never open one of those nasty bad boys


--------------------
:pm:Looking for Penis Envy & Psilocybe Cubensis Cuba spores.:pm:


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InvisibleinskiM
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Re: Hydrogen Peroxide use... [Re: DetectiveLefty]
    #21683239 - 05/15/15 01:55 PM (8 years, 8 months ago)

Quote:

DetectiveLefty said:
don't want spores all over my skin & clothes.



Trichoderma is everywhere, the spores are already all over your skin and clothes, it's in all soil, in the air, it's everywhere.


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InvisibleMad Season
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Re: Hydrogen Peroxide use... [Re: inski]
    #21683250 - 05/15/15 01:58 PM (8 years, 8 months ago)

Indeed! Which is why I believe in agar. Clean inoculation should make clean spawn. Clean spawn should make clean substrates.  Clean substrates should fight off contaminations for a couple months.

If you can have clean colonization, bring on all the contaminations, it'll fight them off. Still always a good idea to reduce # of spores in grow area as much as possible.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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OfflineKingcannon
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Re: Hydrogen Peroxide use... [Re: Kingcannon]
    #21683692 - 05/15/15 03:54 PM (8 years, 8 months ago)

The cakes are starting to cover in tiny shrooms and I think the mycelium have fought off any invaders,they look healthy too.
My original point was experimentation to see if contaminations could be eliminated once introduced, I just love experimentation.
Since starting this hobby I have learnt a lot about bacteria, mould types and growth and find it fascinating almost to the point the mushrooms have become a side line. lol

Despite saying this has been my third grow I have actually had various attempts on other substrates like Warburtons Sandwich Thins with success. I cant help but not follow the path others have travelled, I thought it was just a Psychedelic nuts way.


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InvisiblebodhisattaMDiscordReddit
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Re: Hydrogen Peroxide use... [Re: Kingcannon]
    #21683919 - 05/15/15 05:19 PM (8 years, 8 months ago)

walking through the woods exposes you to more contaminants than any fucked substrate will. nonetheless don't deliberately be stupid with contaminants in your house


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OfflineKingcannon
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Re: Hydrogen Peroxide use... [Re: Kingcannon]
    #21719804 - 05/25/15 06:16 AM (8 years, 8 months ago)

Just an update on shroom progress on the slime mould contaminated cakes. All posts taken into account this is for experimentation on helping cubensis mycelium fight of invading contaminations and the possibilities..  ( we all know we should bin them,,,,, right !)

After fine misting contaminated areas with undiluted 9% hydrogen peroxide the cubensis mycelium returned unaffected. As can be seen the mushroom pins and growth seemed to focus in on these sprayed contaminated areas.

The chunks missing from the cakes are obviously where the slime mould started eating into the cakes until treated.

http://s1346.photobucket.com/user/kingcannon1/B%20plus%20Second%20Attempt/B%20Plus%203rd%20attempt/story


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Offlinecarlatomlinson
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Re: Hydrogen Peroxide use... [Re: Kingcannon]
    #21719814 - 05/25/15 06:21 AM (8 years, 8 months ago)

that is pretty darn cool.  thanks for sharing


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InvisibleMr. Alien
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Re: Hydrogen Peroxide use... [Re: Mad Season]
    #21720035 - 05/25/15 08:21 AM (8 years, 8 months ago)

Quote:

Mad Season said:
Quote:

Kizzle said:
No one is going to die simply because their substrate got contaminated. Not trying to encourage people to keep their contaminated substrates around or anything but no need for them to shit bricks either every time they run into a contaminant either.

If you can't bare to toss it at least move it outdoors.



Although I completely agree, I still think putting every contaminated substrate into the bath tub, sink, etc. and trying to dissect/flush the contams out will eventually result in a hospital visit one day before long.




You don't keep your contaminated substrate indoors because you are afraid of dying but because you don't want your working space contaminated with fungal spores everywhere.

I don't think there is any mold that could harm you, unless you had an allergic reaction to a specific type of mold or mold spores. In that case you would be 1 of millions with an extremely bad luck.

A lot of people live with black mold in their bathrooms unnoticed for years.


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InvisibleMad Season
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Re: Hydrogen Peroxide use... [Re: Mr. Alien]
    #21720042 - 05/25/15 08:28 AM (8 years, 8 months ago)

Quote:

TheEaglesGift said:
Some purple molds, like Fusarium, can kill you





Quote:

TheEaglesGift said:
I'm sure there are others but I don't know for sure. Black/pink/purple are potentially deadly, so I'm very cautious around them. Generally speaking, green/blue molds are usually safe for a human to be around but cultivators should still limit there sporulation in our homes/grow rooms, for obvious reasons. 

I knew a grower from a few years back who almost died from coming into contact with fusarium. He was sick for weeks if not months. This was years ago, so I don't remember all the details. His story, which I know was true, was enough to really set in my mind to never fuck with molds I can't truly identify.




That's kinda silly to think. Like I said don't fuck with molds man.

Fusarium can grow in humans. I'm sure there's other molds and bacteria that could be problematic. If you can't identify it just toss it.


Edited by Mad Season (05/25/15 08:37 AM)


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Invisibleabductee
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Re: Hydrogen Peroxide use... [Re: Kingcannon]
    #21720059 - 05/25/15 08:37 AM (8 years, 8 months ago)

how come those pics are dated 2006 in that link. I thought this was a recent post..lol i'm confused.


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InvisibleMr. Alien
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Re: Hydrogen Peroxide use... [Re: Mad Season]
    #21720061 - 05/25/15 08:38 AM (8 years, 8 months ago)

Quote:

Mad Season said:
Quote:

TheEaglesGift said:
Some purple molds, like Fusarium, can kill you





Quote:

TheEaglesGift said:
I'm sure there are others but I don't know for sure. Black/pink/purple are potentially deadly, so I'm very cautious around them. Generally speaking, green/blue molds are usually safe for a human to be around but cultivators should still limit there sporulation in our homes/grow rooms, for obvious reasons.

I knew a grower from a few years back who almost died from coming into contact with fusarium. He was sick for weeks if not months. This was years ago, so I don't remember all the details. His story, which I know was true, was enough to really set in my mind to never fuck with molds I can't truly identify.




That's kinda silly to think. Like I said don't fuck with molds man.

Fusarium can grow in humans. I'm sure there's other molds and bacteria that could be problematic. If you can't identify it just toss it.




Fusarium is more common in grain spawn not in fruiting substrates. Are we talking about grain spawn or fruiting substrates? I don't think any sane grower would open a pink grain spawn before sterilizing it.


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Invisibleabductee
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Re: Hydrogen Peroxide use... [Re: abductee]
    #21720063 - 05/25/15 08:40 AM (8 years, 8 months ago)

lol never mind.. im baked. I see its a discussion on how you've treated contaminants.


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InvisibleMad Season
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Re: Hydrogen Peroxide use... [Re: Mr. Alien]
    #21720066 - 05/25/15 08:41 AM (8 years, 8 months ago)

I get it. Still though there is grains in substrates. There is a chance the contaminated substrate came from the spawn. They say 9/10 times actually.

This is why you give your jars a shake and recovery before you make the substrate. Molds can be hiding too.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Edited by Mad Season (05/25/15 08:42 AM)


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InvisibleMr. Alien
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Re: Hydrogen Peroxide use... [Re: Mad Season]
    #21720081 - 05/25/15 08:51 AM (8 years, 8 months ago)

From what i know that pink mold is very aggressive it will not remain hidden, it will eat through any mushroom mycelium. You would have to be blind to not spot that pink mold in your spawn before spawn to bulk. I don't think any fruiting substrate common molds are harmful enough to get you to the hospital, even some black molds that are more common in fruting substrates won't kill you for breathing a brief time the spores.


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InvisibleMad Season
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Re: Hydrogen Peroxide use... [Re: Mr. Alien]
    #21720106 - 05/25/15 09:05 AM (8 years, 8 months ago)

I personally wouldn't, but I also don't use dirty spawn. There's lots of people in this world. For educated individuals it wouldn't happen.

Lots of people get molds in their bulk and it's from dirty spawn, personally I always wonder why people miss dirty spawn. But I know there's all types of people.. I wouldn't assume that a guy who's washing every contamination off is educated. Just saying. Like I said before if you're trying to wash off EVERY contamination you see you'll eventually run into a bad one. Why gamble it?


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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OfflineKizzle
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Re: Hydrogen Peroxide use... [Re: Mr. Alien]
    #21720130 - 05/25/15 09:23 AM (8 years, 8 months ago)

Quote:

Mr. Alien said:
From what i know that pink mold is very aggressive it will not remain hidden, it will eat through any mushroom mycelium. You would have to be blind to not spot that pink mold in your spawn before spawn to bulk. I don't think any fruiting substrate common molds are harmful enough to get you to the hospital, even some black molds that are more common in fruting substrates won't kill you for breathing a brief time the spores.



I think you're mistaking mold which was misidentified as mushroom mycelium starting to sporulate as mold eating through mushroom mycelium. Fusarium, which is a common pink/purple mold, is notoriously slow to start sporulating, anywhere from 1-3 weeks after starting to grow depending on the medium. It also grows at about the same rate as mushroom mycelium and looks very similar before sporulation.

Like this


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InvisibleMr. Alien
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Re: Hydrogen Peroxide use... [Re: Kizzle]
    #21720215 - 05/25/15 09:52 AM (8 years, 8 months ago)

Wow that guy got that purple pink mold in all his spawn. Well, then it would be safer if you let pass some time at least 1 week your spawn after full colonization in search for any signs of pink/purple sporulation. It seems that the guy in the picture didn't complete sterilization.


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OfflineKingcannon
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Re: Hydrogen Peroxide use... [Re: abductee]
    #21720279 - 05/25/15 10:14 AM (8 years, 8 months ago)

The camera had a date stamp turned on but the date wasn't set right.These are growing as we speak and all being well will try some tonight or this week.


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Invisibleabductee
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Re: Hydrogen Peroxide use... [Re: Kingcannon]
    #21720286 - 05/25/15 10:18 AM (8 years, 8 months ago)

Awesome man :smile:! the sweet satisfaction of reaping our rewards.


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OfflineKizzle
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Re: Hydrogen Peroxide use... [Re: Mr. Alien]
    #21720403 - 05/25/15 10:51 AM (8 years, 8 months ago)

Quote:

Mr. Alien said:
Wow that guy got that purple pink mold in all his spawn. Well, then it would be safer if you let pass some time at least 1 week your spawn after full colonization in search for any signs of pink/purple sporulation. It seems that the guy in the picture didn't complete sterilization.



From what I've gathered on the contamination forum the majority of cases are from contaminated spores. If you've ever poured a bag of dry grains the dust it releases into the air is rich with the stuff. If it's done near where mushrooms are growing that dust can wind up on the gills and contaminate the spores of any print made from it. Particularly bad for those making spore syringes but on agar it may not have time to sporulate either before it's used.

In grain jars normally it shows up by full colonization so it's not that common to find on bulk substrates. In PF cakes it normally sporulates at about half colonization.


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InvisiblebodhisattaMDiscordReddit
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Re: Hydrogen Peroxide use... [Re: Kizzle]
    #21720469 - 05/25/15 11:12 AM (8 years, 8 months ago)

people are overly concerned about contaminated substrates, health, and contaminating their home. go for a walk in the woods and you're exposed to more contamination than you could ever grow. just don't be a retard about it and you'll be fine. there's no reason to go bio level 4 because you have a pink jar though, that's dumb.


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InvisibleMr. Alien
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Re: Hydrogen Peroxide use... [Re: Kizzle]
    #21720496 - 05/25/15 11:20 AM (8 years, 8 months ago)

Yes i was thinking that too, for getting that mold in all your grain jars of pf tek, it was either a sterilization problem or a problem with the spore solution.

If you use spore solution to inoculate your spawn you may find this mold more often than if you were using agar. For grain spawn it may show the purple/pink color near the end of full colonization, still very hard to not spot it before spawn to bulk? Or we would have more often cases of purple/pink fruiting substrates in the contamination forum.


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InvisibleMr. Alien
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Re: Hydrogen Peroxide use... [Re: bodhisatta]
    #21720505 - 05/25/15 11:23 AM (8 years, 8 months ago)

Quote:

bodhisatta said:
there's no reason to go bio level 4 because you have a pink jar though, that's dumb.




:laugh2:


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InvisibleMad Season
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Re: Hydrogen Peroxide use... [Re: Mr. Alien]
    #21720935 - 05/25/15 01:28 PM (8 years, 8 months ago)

Lol I'm just trying to clarify that I didn't say to go and bleach your room or pc your substrates. I was pointing out the dangers of handling/washing/disecting every contaminated substrate in open air and tried to discourage op from doing it. I'm not saying what you guys say is wrong because I don't go over board for every contam but there is risks in this hobby and it doesn't hurt to make newbies aware.

Like everything in life, no such thing as being too safe.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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Offlinekn33b
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Re: Hydrogen Peroxide use... [Re: Mad Season]
    #21722837 - 05/25/15 09:54 PM (8 years, 8 months ago)

Glad you somehow had good luck with it... Bleach, proper PCing, and clean inoculations are key. Peroxide has only ever 1. Salvaged me some myc by burning off bacteria on horrible projects gone wrong as a whole, and 2. Added more oxygen to the distilled water in my ultrasonic humidifier.


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All written material by this individual is for entertainment purposes only.
All events described or pictures displayed therein are ficticious and do not necessarily reflect the author's opinions or intentions.


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