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hamloaf
Loaf of Fam.

Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma.
Last seen: 1 year, 8 months
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Psilocybe Galindoi Help Please.
#21677004 - 05/13/15 10:42 PM (8 years, 8 months ago) |
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Hey there. Have these 3 - 27 quart minimonos that were spawned to bulk (pasteurized horse manure/vermiculite/gypsum) on the 15th of April, and cased on the 2nd of May, and introduced into fruiting. For the introduction of fruiting, the subs were misted, cased, misted again, and the poly fill was 60-75% removed. The concern is the fact that the casing layer has almost become fully engulfed by the mushroom mycelium yet no pins.

Method used to spawn was the colonized grains were mixed into the bulk substrate materials @ a 1:2 spawn to bulk ratio. It's unknown what the exact temperature range the mycelium has been in, because the temperature hasn't been being monitored, so a thermometer was placed near the tubs a few minutes ago, and the temperature gauge is reading 68F, and 78%RH. If an educated guess had to be made as to what temperature range it's been during the duration of the substrate's life, it would be hypothesized that the temperature range has been between the mid 60's to mid 70's. Can/Will anybody with more experience than me growing this specie explain why no /pinning/fruitings are taking place?
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Sksoul
Pan handler



Registered: 10/31/14
Posts: 397
Loc: Far East
Last seen: 3 years, 6 months
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Re: Psilocybe Galindoi Help Please. [Re: hamloaf]
#21677048 - 05/13/15 10:55 PM (8 years, 8 months ago) |
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Isolate or MS?
I've had 2 multispore Galindoi grows not put out any fruits after a month in fruiting. Many big stones, but no fruits. On these grows my subs kept drying out (I think from the stones stealing water) under the casing. Maybe cut into a corner and see if it has enough moisture. Did you allow the jars to grow stones, or go straight from full colonization to spawning?
My isolates from invitro petri dish stones have fruited without any problem when spawned immediately after colonization finished. Sorry I can't be more help.
-------------------- Like all great travellers, I have seen more than I remember, and remember more than I have seen.
Edited by Sksoul (05/13/15 11:03 PM)
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Achillita
Back to the basics



Registered: 05/26/14
Posts: 4,565
Last seen: 3 years, 10 days
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Re: Psilocybe Galindoi Help Please. [Re: Sksoul]
#21677318 - 05/14/15 12:39 AM (8 years, 8 months ago) |
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Hey hamloaf! I don't have any experience growing P galindoi, but I'm starting a grow very soon so I've been researching!
Anyways, from what I've seen the casing layer isn't supposed to be colonized, my guess it defeats the purpose of a casing layer to provide a microclimate. My far off guess is to possibly try to case it again. What casing mix did you use?
There could be a chance of the genetics not allowing it to fruit. I've read, but not 100% sure, that the fruits grow usually from screlotia. Maybe it needs a bit more time? It sounds like when you applied the casing layer it was still trying to colonize.
Also most grows I've seen in a monotub for galindoi, had no polyfill stuffed in the holes at all. They had a slightly modified "x" hole, or just an open hole. It sounds like it needs another casing layer IMNO (In My Noob Opinion)
Sorry if I couldn't be better help.
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hamloaf
Loaf of Fam.

Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma.
Last seen: 1 year, 8 months
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Re: Psilocybe Galindoi Help Please. [Re: Achillita]
#21677752 - 05/14/15 05:46 AM (8 years, 8 months ago) |
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Thanks you guize for the tips and advise. This is the casing mix that was used. You are correct in your assumption over the purposes, and functions of the casing layer. The fact that the casing layer was so colonized, these subs were allowed 15 days to colonize, and were cased back on the 2nd of May are the factors that cause alarm. That's almost a full month of colonization.
Inoculm used was MS. Makes sense that a genetic crap shoot may be a factor in the non-fruitings of these substrates. Looks like the substrates will be re-cased, and the possible removal of more polyfill will be considered.
Achillita, would you care to share some of the research material you've been reading over this specie?
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blojo02184
Big Red



Registered: 05/15/13
Posts: 3,525
Loc: Maine
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Re: Psilocybe Galindoi Help Please. [Re: hamloaf]
#21677806 - 05/14/15 06:15 AM (8 years, 8 months ago) |
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If you have it in a liner, try to rip up some sod, and put the sub just below the sod in your yard... might get something.
Sad to hear about the sub. I've had with happen to me a few times and gave up on fruits for awhile.
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Grey
⇜ ✯ ⇝



Registered: 11/06/14
Posts: 6,223
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Re: Psilocybe Galindoi Help Please. [Re: blojo02184]
#21677912 - 05/14/15 07:14 AM (8 years, 8 months ago) |
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I'm not sure who it was, maybe Blindingleaf, but he had a mexicana tub not fruiting. He dunked it, and then it fruited.
In his thread, Stonesun mentions that when fruiting from a brf cake to let it dry out for a night then dunk. It is supposed to fruit better.
Maybe the idea is that the mycelium will assume that survival of the sclerotia is about run out from drying. Your sub is probably just happy supporting the stones and sees no reason to send forth fruit.
--------------------
AMU Q&A If you don't have a plan of your own, you'll become a part of somebody else's.
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stonesun
Sclerotia Aficionado




Registered: 05/22/09
Posts: 5,444
Loc: 64º08'00"N 21º56'00"W
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Re: Psilocybe Galindoi Help Please. [Re: Achillita]
#21678261 - 05/14/15 09:34 AM (8 years, 8 months ago) |
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Quote:
Grey said: Your sub is probably just happy supporting the stones and sees no reason to send forth fruit.
This is what I think happened most likely. Ham, you said it took 15 days to colonize? My last attempt took only about a week. Was the substrate 100% before, and you let it consolidate, or it really took over two weeks to fully colonize? I found that it is essential to quickly case after full colonization, and fruit it immediately (I know you did that) for best fruiting performance. In most cases whenever I forgot to case and let the substrate sit for a few more days after full colonization, the mycelium "focused" more on producing sclerotia than fruits. Eventually it fruited, but poorly. Your temperatures are pretty low as well, but I don't think that's the real problem.
Quote:
Achillita said: I've read, but not 100% sure, that the fruits grow usually from screlotia.
The fruits are almost never grow out of sclerotia. They can and will sometimes, but very seldom.
Quote:
Achillita said: Also most grows I've seen in a monotub for galindoi, had no polyfill stuffed in the holes at all. They had a slightly modified "x" hole, or just an open hole.
I have almost all holes stuffed even in fruiting, except one or two. I usually have one upper hole open the rest stuffed. When I feel that it needs more air, I open up the other upper hole.

But the lower holes are always stay stuffed. Hope it helps a little.
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takofako
Stranger



Registered: 01/05/15
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Re: Psilocybe Galindoi Help Please. [Re: stonesun]
#21678651 - 05/14/15 11:50 AM (8 years, 8 months ago) |
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monitoring
-------------------- Sorry about spelling, english is not my main language My Grow Log and My Teks
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hamloaf
Loaf of Fam.


Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma.
Last seen: 1 year, 8 months
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Re: Psilocybe Galindoi Help Please. [Re: stonesun]
#21678674 - 05/14/15 12:00 PM (8 years, 8 months ago) |
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Quote:
Ham, you said it took 15 days to colonize? My last attempt took only about a week. Was the substrate 100% before, and you let it consolidate, or it really took over two weeks to fully colonize?
Yessir, you are correct. Technically full colonization of the substrate was achieved at about the 8 day mark, then a consolidation period was allotted. Substrates are only between 1-2 inches deep.
Quote:
I found that it is essential to quickly case after full colonization, and fruit it immediately (I know you did that) for best fruiting performance. In most cases whenever I forgot to case and let the substrate sit for a few more days after full colonization, the mycelium "focused" more on producing sclerotia than fruits. Eventually it fruited, but poorly. Your temperatures are pretty low as well, but I don't think that's the real problem.
This is EXACTLY what I needed to know. Thanks, man for your insight & wisdom. Nah, the substrates were allowed to consolidate after full colonization was reached. Figured that's the mush cult thing to do, because there really isn't much cultivation information on the internet, or any where for that matter over how to cultivate a lot of "active" species of mushrooms, but I'm not too tek saavy. All there was to go by is your amazing grow guide (which btw should be published into soft/hardback covered book), all the posts on this forum, as well as, other topic of interest related websites, and to read off of wikipedia about how they grow in the wild. Have 2 half gallon jars of this same MS Psilocybe Galindoi culture generated from LC about 2, and a half months out from inoculation killin' it in the stone production department. These jars will be the last chance to fruit this specie with out having to go back to stock cultures, and/or spores.

In between the generation of the half gallon jars, and these minimono's are 2 - 1, and a half gallon, sterilized horse manure bulk subs, each inoculated with 1 quart of fully colonized, stone producing Psilocybe Galindoi mushroom mycelium.

With the information provided, what would ye say the best coarse of action would be to see fruits from these substrates in the minimonos happen, stoneson?? Thanks again for kickin' your knowledge over the issue.
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takofako
Stranger



Registered: 01/05/15
Posts: 209
Last seen: 6 years, 10 months
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Re: Psilocybe Galindoi Help Please. [Re: hamloaf]
#21678702 - 05/14/15 12:07 PM (8 years, 8 months ago) |
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People are saying that they can grow from myc which they get from stones (they cut pieces from stones then put that on agar) . If your last chance is these jars maybe you can save 2-3 stones to put on agar, then spawn the rest. I am seeing around that people even get myc from store-bought stones. Maybe this can be an alternative and chance saving way. I am no pro by the way
-------------------- Sorry about spelling, english is not my main language My Grow Log and My Teks
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hamloaf
Loaf of Fam.


Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma.
Last seen: 1 year, 8 months
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Re: Psilocybe Galindoi Help Please. [Re: takofako]
#21678733 - 05/14/15 12:18 PM (8 years, 8 months ago) |
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Hey - takofako. Those are good suggestions. As an added bonus, the mycelium generated from freshly harvested stone will be fresher than that from store bought stuff, therefore should produce a more vigorous culture, than that taken from store bought product. Good. Thanks for the tips and advise. Appreciate it.
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takofako
Stranger



Registered: 01/05/15
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Re: Psilocybe Galindoi Help Please. [Re: hamloaf]
#21679559 - 05/14/15 04:27 PM (8 years, 8 months ago) |
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one more thing i have heard from bw86, he even got a fruit from a stone with just covering it with sand. But this is not a miracle i guess because they produce it for that, surviving. And some other information that i have heard says you can dry a sclerotia then rehydrate it, even after this it can fruit and if it can fruit, that means rehyrated stones can produce myc on agar too. so maybe you can save a couple more stones dried for later uses like slants etc. but maybe these shouldnt be like cracker. plant seeds have estimated %30 RH in them if i remember correctly so this protect them from molds but keeps them alive, so it is important that how much you dry your stones if you like to use this way but i dont know maybe they are very strong and they can even produce myc or fruit after cracker dry level. (i think cracker dry means %10 RH because i have 20 kilograms of silicagel and put a hydrometer in it, it showed %10 even drier then a desert , so maybe you shouldnt dry them to %10). anyway i dont know, i talked too much
Edited by takofako (05/14/15 04:43 PM)
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Aero
Orea


Registered: 11/01/13
Posts: 2,253
Last seen: 1 year, 2 months
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Re: Psilocybe Galindoi Help Please. [Re: takofako]
#21679686 - 05/14/15 04:49 PM (8 years, 8 months ago) |
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store bought sclerotia is still fresh in my case i sampled different batches and all worked fine
Cloning store bought sclerotia
-------------------- SPREAD THE SPORES
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Buck513

Registered: 04/17/14
Posts: 5,682
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Re: Psilocybe Galindoi Help Please. [Re: Aero]
#21680199 - 05/14/15 06:53 PM (8 years, 8 months ago) |
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-------------------- Fail to plan and you plan to fail. Enter the Ban Lottery
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hamloaf
Loaf of Fam.


Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma.
Last seen: 1 year, 8 months
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Re: Psilocybe Galindoi Help Please. [Re: Buck513]
#21693563 - 05/18/15 06:56 AM (8 years, 8 months ago) |
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First pins have arrived today. They are very sparse but, hey, at least the potential to get prints in nearing completion. Getting prints being the end goal of the cultivator. Plus a better idea over how to fruit this specie has been gained.

Nothing has been done to the fruiting chambers, and/or substrates themselves to encourage fruiting. One interesting note is that the climate's ambient temperate range has increased over the last 3 days from being 55F at night 68-70F during the day for about three weeks to 80F days and 65F nights.
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Achillita
Back to the basics



Registered: 05/26/14
Posts: 4,565
Last seen: 3 years, 10 days
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Re: Psilocybe Galindoi Help Please. [Re: hamloaf]
#21693604 - 05/18/15 07:29 AM (8 years, 8 months ago) |
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Maybe it pinned because the temperatures were similar to spring time? Anyways nice job hamloaf!
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Aero
Orea


Registered: 11/01/13
Posts: 2,253
Last seen: 1 year, 2 months
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Re: Psilocybe Galindoi Help Please. [Re: hamloaf]
#21693619 - 05/18/15 07:45 AM (8 years, 8 months ago) |
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Quote:
hamloaf said: First pins have arrived today. They are very sparse but, hey, at least the potential to get prints in nearing completion. Getting prints being the end goal of the cultivator. Plus a better idea over how to fruit this specie has been gained.

Nothing has been done to the fruiting chambers, and/or substrates themselves to encourage fruiting. One interesting note is that the climate's ambient temperate range has increased over the last 3 days from being 55F at night 68-70F during the day for about three weeks to 80F days and 65F nights.
sweet !!! and yea 75-78 is preferable
-------------------- SPREAD THE SPORES
Edited by Aero (05/18/15 08:00 AM)
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