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Offlinelordkiwi
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Registered: 05/13/15
Posts: 3
Last seen: 7 years, 4 months
Culturing on Agar
    #21675596 - 05/13/15 05:27 PM (8 years, 8 months ago)

Ok a little background.  I want to inoculate my lawn and garden with several cultures.  I ordered liquid cultures online and 1 chanterelle culture on dried grains.

Last fall I made couple of grain spawn bags and inoculated with Straphoria WineCap.  One success one failure and I transferred the success to a couple of bags of wood chips to mature over winter that I will add to the garden plot with more chips.  Inspecting the woodchip bags over winter showed definite transference from the spawn to the chips so I am happy with that.  But with only 1qt of spawn I think I disadvantaged my self.

Anyway I am at it again and I thought I might have better luck transferring to agar then building a liquid culture then finally to grain spawn or use a canister sprayer. 

So hear I go. I got a 20 dish Nutrient Agar kit.  Mix it up autoclave it in my pressure canner @ 15psi.  Fill my first 6 dishes and here are my results.  I think it went well.  The liquid cultures all appear  to be fine.  The dry seed is all contam which is not complexly unexpected.

Questions what should I do next.  The Petri dishes do not close anywhere near securely. I have some 3m Transpore or 3m Durapore tape, no micropore though , I could seal them with.  I didn't touch them or anything but they look dry. Should I pipe some distilled water over them before I tape them up.  Or should I use some pedialyte. 
Finally the Chantrelle grain spawn is triched out however there are two kernels that show no sign of contamination should I excise them and see what they do in a different dish?



Edited by lordkiwi (05/13/15 05:31 PM)


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