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spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 7 years, 4 months
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Re: [IN PROGRESS] Step By Step Guide for Beginners using Agar!! [Re: MysticPop]
#21760618 - 06/04/15 07:59 AM (9 years, 7 months ago) |
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as this is your first time using agar maybe it should be named a step by step guide BY a beginner? You wouldn't wanna tell other newbs to follow your steps if you're not even sure they work out yet would you?
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MysticPop
AlienInWonderland



Registered: 05/05/15
Posts: 132
Last seen: 1 year, 2 months
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Re: [IN PROGRESS] Step By Step Guide for Beginners using Agar!! [Re: spacechildo]
#21760620 - 06/04/15 08:01 AM (9 years, 7 months ago) |
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Agreed., will rename it to step by step guide for beginners by a beginner using agar
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MysticPop
AlienInWonderland



Registered: 05/05/15
Posts: 132
Last seen: 1 year, 2 months
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Re: [IN PROGRESS] Step By Step Guide for Beginners using Agar!! [Re: MysticPop]
#21760627 - 06/04/15 08:06 AM (9 years, 7 months ago) |
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Name changed..
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MysticPop
AlienInWonderland



Registered: 05/05/15
Posts: 132
Last seen: 1 year, 2 months
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Re: [IN PROGRESS] Step By Step Guide for Beginners using Agar!! [Re: MysticPop]
#21760630 - 06/04/15 08:06 AM (9 years, 7 months ago) |
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New name posts
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Grey
⇜ ✯ ⇝



Registered: 11/06/14
Posts: 6,223
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Re: [IN PROGRESS] Step By Step Guide for Beginners using Agar!! [Re: MysticPop]
#21761177 - 06/04/15 11:03 AM (9 years, 7 months ago) |
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The mycelium is just eating the food dye.
--------------------
AMU Q&A
If you don't have a plan of your own, you'll become a part of somebody else's.
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MysticPop
AlienInWonderland



Registered: 05/05/15
Posts: 132
Last seen: 1 year, 2 months
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Re: [IN PROGRESS] Step By Step Guide for Beginners BY a Beginner using Agar!! [Re: MysticPop]
#21761548 - 06/04/15 12:50 PM (9 years, 7 months ago) |
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Are you sure its not bacteria? I am getting mixed responses.. Anyways i shall be doing agar to agar transfer..
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spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
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Re: [IN PROGRESS] Step By Step Guide for Beginners BY a Beginner using Agar!! [Re: MysticPop]
#21761585 - 06/04/15 12:57 PM (9 years, 7 months ago) |
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Quote:
MysticPop said: I am getting mixed responses..
where?  its painfully obvious to anyone who's had food color in their agar that this is perfectly normal...
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MysticPop
AlienInWonderland



Registered: 05/05/15
Posts: 132
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Re: [IN PROGRESS] Step By Step Guide for Beginners BY a Beginner using Agar!! [Re: spacechildo]
#21761767 - 06/04/15 01:45 PM (9 years, 7 months ago) |
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I got some replies in PM. Some said its normal and some said its bacterial contamination.. So was confused..
Anyways... what do experts suggest I do now? Is my agar ready for grain inoculation or should i do another transfer and then do grain inoculation?
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spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
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Re: [IN PROGRESS] Step By Step Guide for Beginners BY a Beginner using Agar!! [Re: MysticPop]
#21761821 - 06/04/15 01:59 PM (9 years, 7 months ago) |
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people who have to pm their replies probably knows their advice would be shot down by people with actual experience. I'd take all replies coming in pm's with an even larger grain of salt than replies given on the boards.
you can do whatever you want with the agar plates. the pics of the myc is way out of focus for me to tell whether they're clean or not but its your tek so you do whatever you want your tek to show man!
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MysticPop
AlienInWonderland



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Re: [IN PROGRESS] Step By Step Guide for Beginners BY a Beginner using Agar!! [Re: spacechildo]
#21761833 - 06/04/15 02:03 PM (9 years, 7 months ago) |
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Its not about show.. I want to do it right..
Is it safe to open these plates in open air to take more focussed pics.. Also all the learning from these queries will be consolidated in the tek.. so that people who follow this in future are not confused.
I can post more clear pics..if i can open the glad rounds.. the material of the glad round is translucent and hence the cam is not able to focus..
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Grey
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Registered: 11/06/14
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Re: [IN PROGRESS] Step By Step Guide for Beginners BY a Beginner using Agar!! [Re: MysticPop]
#21761869 - 06/04/15 02:14 PM (9 years, 7 months ago) |
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If the color is getting eaten up, those plates need to be used/transferred before the mycelium runs out of nutrients.
Don't open them in open air. You can open them in a SAB. If you have headphones for your phone that has the little microphone w/ button on it you can use that button to take a picture. Just turn on your phone camera, set the phone on the top of the SAB looking in, with both hands in the SAB open the plate, and press the little button with your mouth or some other creative way. I've done it before, but now my SAB is all scratched up so it's hard to focus.
--------------------
AMU Q&A
If you don't have a plan of your own, you'll become a part of somebody else's.
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MysticPop
AlienInWonderland



Registered: 05/05/15
Posts: 132
Last seen: 1 year, 2 months
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Re: [IN PROGRESS] Step By Step Guide for Beginners BY a Beginner using Agar!! [Re: Grey]
#21761890 - 06/04/15 02:19 PM (9 years, 7 months ago) |
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Before i inoculate grain jars, i just want to be sure if the myc is correct. @Grey: Looking at the current pics, can you advise if this is good enough?
Else Once i am done work i will go and open it in sab and post some pics. Based on the advise I get, i would either inoculate the grain or do a transfer.
Thank you all for the valuable feedback.
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Grey
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Registered: 11/06/14
Posts: 6,223
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Re: [IN PROGRESS] Step By Step Guide for Beginners using Agar!! [Re: MysticPop]
#21761916 - 06/04/15 02:27 PM (9 years, 7 months ago) |
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I can't say I haven't used a plate like that, but I prefer to have clean/less random looking growth. Make a transfer to be safe, but you could still use them afterwards if your comfortable with your sterile technique.
--------------------
AMU Q&A
If you don't have a plan of your own, you'll become a part of somebody else's.
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MysticPop
AlienInWonderland



Registered: 05/05/15
Posts: 132
Last seen: 1 year, 2 months
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Re: [IN PROGRESS] Step By Step Guide for Beginners using Agar!! [Re: Grey]
#21761925 - 06/04/15 02:28 PM (9 years, 7 months ago) |
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OK Then.. I will do a transfer then to MEYA type agar in petri dishes and will wait for them to grow..
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MysticPop
AlienInWonderland



Registered: 05/05/15
Posts: 132
Last seen: 1 year, 2 months
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Re: [IN PROGRESS] Step By Step Guide for Beginners using Agar!! [Re: MysticPop]
#21807201 - 06/14/15 05:01 PM (9 years, 7 months ago) |
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added pics for agar to agar transfer
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MysticPop
AlienInWonderland



Registered: 05/05/15
Posts: 132
Last seen: 1 year, 2 months
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Re: [IN PROGRESS] Step By Step Guide for Beginners using Agar!! [Re: MysticPop]
#21807312 - 06/14/15 05:31 PM (9 years, 7 months ago) |
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Hey experts,
Any comments on the latest growth pics?
Cheers
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spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 7 years, 4 months
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Re: [IN PROGRESS] Step By Step Guide for Beginners using Agar!! [Re: MysticPop]
#21807330 - 06/14/15 05:36 PM (9 years, 7 months ago) |
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lot of nasty stuff going on there, do more transfers.
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MysticPop
AlienInWonderland



Registered: 05/05/15
Posts: 132
Last seen: 1 year, 2 months
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Re: [IN PROGRESS] Step By Step Guide for Beginners using Agar!! [Re: spacechildo]
#21807343 - 06/14/15 05:39 PM (9 years, 7 months ago) |
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Transfers from what section do you recommend? Also should these transfers into multiple plates or to a single plate?
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spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 7 years, 4 months
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Re: [IN PROGRESS] Step By Step Guide for Beginners using Agar!! [Re: MysticPop]
#21807350 - 06/14/15 05:40 PM (9 years, 7 months ago) |
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just take a tiny piece of healthy growth and place onto a new plate.
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MysticPop
AlienInWonderland



Registered: 05/05/15
Posts: 132
Last seen: 1 year, 2 months
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Re: [IN PROGRESS] Step By Step Guide for Beginners using Agar!! [Re: spacechildo]
#21807368 - 06/14/15 05:46 PM (9 years, 7 months ago) |
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I have the following questions:-
1. What are the health growth Sections as per you? 2. How many plates do you suggest to transfer to? 3. Do i discard the rest of the plates or let them grow?
Cheers
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