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OfflineMysticPop
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Registered: 05/05/15
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[IN PROGRESS] Step By Step Cultivation Guide for Beginners BY a Beginner using Agar and Monotub!!
    #21670939 - 05/12/15 05:10 PM (9 years, 8 months ago)

Hi all,

PLEASE NOTE:- I SHALL UPDATE THIS AS AND WHEN I DO STUFF.

Started this hobby for fun and thought of documenting the steps to make it easier for the beginners. This contains links to the teks used. Thanks to all the people who wrote all those teks.

Things Used :- (* is optional). You may choose to buy from anywhere you like or prefer. IN NO WAY I AM PROMOTING ANY VENDOR ETC. Provided links just to help people to identify what they are looking for.

1. 110 QT Sterlite - 1 Qty - Required for SAB
2. 66 QT Sterlite - 2 QTY - Required for Monotub
3. 4" PVC Closeout with Plug - 2 QTY - Required for Hand Holes and its cover in SAB. Sometimes you may buy these separately.
4. Silicone Tube - 1 QTY - To Seal Outer part of Hand holes and SAB.
5. Silicone II tube - 1 Qty- to seal the inner part of hand holes and SAB.
6. Tyvek Sleeves - 1 Pair - To cover the skinny end of the hand hole.
7. Tyvek Whole Coverall* - 1 Qty - To Cover Yourself while performing sterile work.
8. 3-9/16" to 4-1/2" Stainless steel clamps - 2 Qty - To hold Tyvek sleeves to hand holes.
9. 5 Gallons Buckets - 2 QTY - to Wash your Substrate etc
10. Fiberglass Insect Screen - 1 Small Roll - Can be used as Strainers for Multiple purposes. Used to Dry the Grain
11. Agar
12. Mini Glad Rounds. Put a hole in the lid using 1/4 inch drill bit.
13. Green Food Coloring. As suggested in PatsyWhyte's Agar Tek Guide.
14. Mash Potatoes Flakes
15. Karo
16. Micropore Tape :- This is to tape on the hole your drill in the mini glad round lid.
17. Aluminium Foil: To cover before PC. I hope you dont need  a link to this. :laugh:
18. PC
19. Gloves - Get it in any pharmacy
20. 70% Isopropyl alcohol - Get it in any pharmacy.
21. Butane Torch - Used to heat the syringe needle before inoculating.
22. Butane Fuel - To fill your butane light.
23. Light Malt Extract
24. Nutritional Yeast
25. Peptone
26. Ground Rye Grain - you can get this at local grocery or grind you substrate rye berries.
27. Petri Dishes
28. Scalpel # 11
29. Parafilm
30. Polycarbonate Erlenmeyer Flask
31. Organic Rye berries
32. Garden Gypsum
33. Wide Mouth Quart Mason Jars
34. Synthetic Filter Discs

WILL KEEP UPDATING AS AND WHEN I USE STUFF!!


1. Make Still Air Box. To Drill Holes I went to Home Depot and used their Drills on Display :smile:. The Home Depot Manager was kind enough to let me use their hole saw and arbor for free. They even helped me put the hole in the tote.



2. Make your Agar Mix for Inoculation. I personally used PatsyWhytes's Agar Tek.


3. Prepare Your SAB Workspace.

4a. Using this video to inoculate Agar with Spore Syringes. This is first time attempt. If this fails I shall use spore print. Wore tyvek coverall and gloves. Sprayed everything with Isopropyl Alcohol

Stored the mini glads in closet after inoculation.

After 3 weeks of inoculation, I took some pics. Here it is. Dont Know what the yellow thing on the bottom of the container is. Need Help here:-



Agar to Agar Transfer :-

In the Pics above, the agar transferred from Green to Yellow. This is just Mycelium waste and is perfectly normal when you add color to your agar. It is not Bacteria growth. The agar is loosing nutrients and eating the color. Its time to do a transfer. (Thanks for all the experts- Numero, SpaceHildo, Grey and others) for guiding me here.

On 06/05/2015 I did Agar to Agar Transfer. This time I used MEYPA as per the recipe from Paul Stamets Book:-

For 1 Liter of water :-
20 gm Light Malt Extract
2 Gm Yeast
5 gm Ground rye grain
5 gm Peptone
20 gm Agar

Video Link to make your Agar Mix.

Mix ingredients and put the mixed contents in erlenmeyer Flask. Then Add water. Screw the top with a vent cap. Then PC at 15 psi for 30 mins. Once sterilized let the PC come back to 1 Psi naturally. After that transfer the flask to your sab along with petri dishes. Let it cool down and while the agar is still liquid pour your petri dishes. Once the Agar solidify make the transfer using the video Agar to Agar Transfer

I made enough only 250 ml of solution and poured the petri dishes. Once agar to agar transfer was made in SAB, I sealed the Petri dishes using Parafilm. Some people also use saran wrap instead of parafilm.

As of 06/14/2015 the growth looks as below.



As of 06/17 The growth in good plates is as follows:-


Finally I decided to use the following plates and sectors for Grain Inoculation:-



Grain Preparation:-

I Used the Rye berries as grains here. use the following step by step guide for Rye Berry Preparation

http://www.shroomery.org/forums/showflat.php/Number/20048765#20048765

While the grains were drying I prepared the Lids Using the following :-

http://www.shroomery.org/forums/showflat.php/Number/20048747#20048747



Edited by MysticPop (07/06/15 04:24 PM)

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OfflinecronicrMFacebook
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Re: [IN PROGRESS] Step By Step Guide for Beginners using Agar!! [Re: MysticPop]
    #21670983 - 05/12/15 05:21 PM (9 years, 8 months ago)

:bottledup:  :monopoly:  :puppet:


--------------------
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InvisibleGrey
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Re: [IN PROGRESS] Step By Step Guide for Beginners using Agar!! [Re: cronicr]
    #21670998 - 05/12/15 05:27 PM (9 years, 8 months ago)

:derp:


--------------------


:takingnotes:  AMU Q&A  :takingnotes:


If you don't have a plan of your own, you'll become a part of somebody else's.

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Offlinenooberst
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Re: [IN PROGRESS] Step By Step Guide for Beginners using Agar!! [Re: Grey]
    #21671071 - 05/12/15 05:47 PM (9 years, 8 months ago)

A swimmer! But sweet can't wait.


--------------------

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OfflineMysticPop
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Re: [IN PROGRESS] Step By Step Guide for Beginners using Agar!! [Re: nooberst]
    #21685183 - 05/15/15 11:04 PM (9 years, 8 months ago)

Updated with next step and some new pics..

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InvisibleGrey
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Re: [IN PROGRESS] Step By Step Guide for Beginners using Agar!! [Re: MysticPop]
    #21685206 - 05/15/15 11:11 PM (9 years, 8 months ago)

It says SWIM, then says "things I use".

I don't see too many working display drills at my hardware stores. I can't imagine they would be cool with you drilling that tote.


--------------------


:takingnotes:  AMU Q&A  :takingnotes:


If you don't have a plan of your own, you'll become a part of somebody else's.

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Invisiblewowimflabbergasted
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Re: [IN PROGRESS] Step By Step Guide for Beginners using Agar!! [Re: Grey]
    #21685220 - 05/15/15 11:15 PM (9 years, 8 months ago)

Interesting.


--------------------
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InvisibleMad Season
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Re: [IN PROGRESS] Step By Step Guide for Beginners using Agar!! [Re: wowimflabbergasted]
    #21685259 - 05/15/15 11:26 PM (9 years, 8 months ago)

Haha oh grey. Would be funny to see a guy huddled in a corner at home hardware drilling holes into his totes. I'd be like I know where you're doing :wink:. On a side note my home Depot has a tool rental area. Could just rent a drill and hole saws for a day for pretty cheap


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.

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OfflineMysticPop
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Re: [IN PROGRESS] Step By Step Guide for Beginners using Agar!! [Re: Mad Season]
    #21685436 - 05/16/15 12:15 AM (9 years, 8 months ago)

yes.. You can do whatever u want.. The aim is to get 2 holes in that tote..HAHAH!!:ooo:

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OfflineDeadZeppelin92
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Re: [IN PROGRESS] Step By Step Guide for Beginners using Agar!! [Re: Mad Season]
    #21685447 - 05/16/15 12:17 AM (9 years, 8 months ago)

:threadmonitor:


--------------------
I can't help you with your troubles, if you won't help with mine!

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Invisiblewowimflabbergasted
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Re: [IN PROGRESS] Step By Step Guide for Beginners using Agar!! [Re: DeadZeppelin92]
    #21686530 - 05/16/15 10:20 AM (9 years, 8 months ago)

SWIM is gay  :eatadick:


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OfflineSksoul
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Re: [IN PROGRESS] Step By Step Guide for Beginners using Agar!! [Re: wowimflabbergasted]
    #21686734 - 05/16/15 11:17 AM (9 years, 8 months ago)

In elementary school this would have gotten an "E" for "good effort." But you cant use "swim" and "I" in the same post/sentence.


--------------------
Like all great travellers, I have seen more than I remember, and remember more than I have seen.

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InvisibleMad Season
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Re: [IN PROGRESS] Step By Step Guide for Beginners using Agar!! [Re: Sksoul]
    #21686739 - 05/16/15 11:19 AM (9 years, 8 months ago)

I always just interpret it as someone who is me


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.

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OfflineGreenRabbit
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Re: [IN PROGRESS] Step By Step Guide for Beginners using Agar!! [Re: Mad Season]
    #21686747 - 05/16/15 11:22 AM (9 years, 8 months ago)

Quote:

Mad Season said:
I always just interpret it as someone who is me




lol. I like that. I only use SWIM when I am out of town and it literally is SWIM and I'd rather not explain who they are haha.

You can't protect yourself but you can protect your friends.. Just don't post an active log if you're paranoid

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OfflineMysticPop
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Re: [IN PROGRESS] Step By Step Guide for Beginners using Agar!! [Re: GreenRabbit]
    #21687082 - 05/16/15 01:28 PM (9 years, 8 months ago)

Dont know about the Hate around using SWIM but here you go..modified the post to first person  :P

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OfflineMysticPop
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Re: [IN PROGRESS] Step By Step Guide for Beginners using Agar!! [Re: MysticPop]
    #21759746 - 06/03/15 11:19 PM (9 years, 7 months ago)

I have the following questions guys.. Any help would be appreciated...:smile:

1. If you see the pics, the mycelium on top itself is pure white. However when i see the mini glad from below it has turned yellow. I am not sure if it is contaminant or some mycelium excreta?

2. if the below is contaminated can i still use the mycelium from the top?

3. If yellow is contaminant, i still have some portion that is still green. Can i salvage that and use that for further process?

4. The Next process from any part if salvaged.. Will it be creating more agar plates or inoculating the grain jar?



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OfflineMysticPop
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Re: [IN PROGRESS] Step By Step Guide for Beginners using Agar!! [Re: MysticPop]
    #21759970 - 06/04/15 12:52 AM (9 years, 7 months ago)

Bump!

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Invisiblewowimflabbergasted
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Re: [IN PROGRESS] Step By Step Guide for Beginners using Agar!! [Re: MysticPop]
    #21760572 - 06/04/15 07:35 AM (9 years, 7 months ago)

Don't bump within 24 hours. :rules:


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Offlinespacechildo
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Re: [IN PROGRESS] Step By Step Guide for Beginners using Agar!! [Re: MysticPop]
    #21760600 - 06/04/15 07:49 AM (9 years, 7 months ago)

is this a TEK or a questions thread?

should you be guiding new growers if you are a new grower yourself?

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OfflineMysticPop
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Re: [IN PROGRESS] Step By Step Guide for Beginners using Agar!! [Re: spacechildo]
    #21760607 - 06/04/15 07:52 AM (9 years, 7 months ago)

Well a teacher can always learn from students and be better for the future.  I am writing this as beginner.. Hence documenting all intricate details etc.. My queries and replies from experts will only add value to the guide..

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