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ShroomWhore2
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Registered: 11/12/14
Posts: 7
Last seen: 8 years, 7 months
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Agar - No growth after new recipe
#21666416 - 05/11/15 05:23 PM (9 years, 8 days ago) |
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Hey everyone.
I've been using a recipe of 10g DME, 2g yeast, 9g agar-agar and 500ml distilled water and after initial failure with my method have been successfully growing and isolating the keepers creepers (labelled as Blue Meanie) and Australian cubensis strains.
After 4 transfers with the Keepers Creepers and 3 with the Aussie I was ready for a final transfer to a petri dish and was going to put the final transfer into test tubes and put the remaining wedges into grain jars to fruit and compare. I made new petri dishes with a recipe of 7g DME, 1g yeast, 9g agar-agar and 500ml distilled water as the mycelium was kinda fluffy and I read that reducing the nutrients will bring more rizomorphic growth and easier to isolate.
It has been 6 days since I transfered the pieces of mycelium to the new dishes and so far the mycelium hasn't even grown off the wedge itself. The only difference is the recipe and I wondered if there wasn't enough nutrients for the mycelium to grow into it so didn't even move. Just typing that sounds ridiculous! I have read mention of mycelium getting used to the nutrients and not growing but I can't believe that it the problem and I'm not sure that is as true for this species. I planned to pull the previous dishes out of the fridge and cut wedges into some of the old recipe dishes to see if it comes good but that means I have 20 petri dishes here that are useless. None of these dishes show any sign of contamination. My process has been meticulously reviewed and refined after my first 20 dishes went horribly bad. I've had exceptional success since.
Many thanks for your time and suggestions. This is my first venture with agar.
SW.
This is an example of one of the wedges that has not done anything more than just get a little fluffy.

These are the 4 previous dishes I isolated from. As you can see my method has worked well and this was only 6 and 7 days after transfer from the previous dishes.
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Re: Agar - No growth after new recipe [Re: ShroomWhore2]
#21666459 - 05/11/15 05:36 PM (9 years, 8 days ago) |
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it's likely just happy where it is for now. Once it uses the last of the nutrition from the wedge of agar it is sitting on it should jump down to look for more food.
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ShroomWhore2
Stranger


Registered: 11/12/14
Posts: 7
Last seen: 8 years, 7 months
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Re: Agar - No growth after new recipe [Re: MudaFuka]
#21666555 - 05/11/15 06:04 PM (9 years, 8 days ago) |
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Thanks MudaFuka but all other dishes by this time were well and truly growing, as per the picture with the 4 dishes which were the same period. I have 4 new dishes and they are all exactly the same - which is nothing like the previous dishes. I would not expect all 12 wedges cut from 12 different places from 4 previous dishes from 2 different strains to behave exactly the same. I wondered if the air in the dishes was off or not as rich and causing them to stall?
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