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Amazon Shop for: ½ Pint Jars, Agar, Petri Dish

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Offlinekjb1891
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Agar Questions
    #2164664 - 12/06/03 06:18 PM (13 years, 1 month ago)

1.) How long does it usually take for a live tissue culture to colonize agar on petri dishes?

2.) When petri dishes are finished colonizing, what is the best way to store them until use?

3.) Once the agar is finished, How much mycelium culture should be used to inoculate a pint jar of grain?



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Invisiblez@z.com
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Re: Agar Questions [Re: kjb1891]
    #2164693 - 12/06/03 06:34 PM (13 years, 1 month ago)

Quote:

1.) How long does it usually take for a live tissue culture to colonize agar on petri dishes?



Usually takes about 2 weeks for me. You can use it before the entire thing is colonized though.

Quote:

2.) When petri dishes are finished colonizing, what is the best way to store them until use?



Ziplock bag in the fridge.

Quote:

3.) Once the agar is finished, How much mycelium culture should be used to inoculate a pint jar of grain?



I use a small piece. About 1x1cm. Sometimes smaller.


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InvisibleautomanM
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Re: Agar Questions [Re: kjb1891]
    #2165543 - 12/07/03 01:17 AM (13 years, 1 month ago)

i inoculate 3 jars from 1 dish because i like faster colonization times, but that's just me.


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Offlinekjb1891
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Re: Agar Questions [Re: automan]
    #2166305 - 12/07/03 01:18 PM (13 years, 1 month ago)

Which would finish quicker in anyone's opinion? Taking a fully colonized agar dish a taking 12 small cultures from it and inoculating 12 jars of grain, or taking say 4 larger cultures and inoculating 4 jars of grains, then taking those 4 jars when colonized and doing G2G transfers for 12 jars?


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Offlineshirley knott
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Re: Agar Questions [Re: kjb1891]
    #2166320 - 12/07/03 01:33 PM (13 years, 1 month ago)

the first would be quicker - only one generation to grow.
why not grow 12 jars, then use ten as spawn, and the other two to g2g another 10 jars. perpetual motion. :cool:


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Offlinekjb1891
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Re: Agar Questions [Re: shirley knott]
    #2166361 - 12/07/03 01:52 PM (13 years, 1 month ago)

In anyone's opinion, how much faster growth and how much more yield do you usually get using a pure culture strain instead of multispore?


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Offlinekjb1891
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Re: Agar Questions [Re: kjb1891]
    #2168691 - 12/08/03 12:11 PM (13 years, 1 month ago)

bump


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Invisiblemicro
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Re: Agar Questions [Re: kjb1891]
    #2168707 - 12/08/03 12:18 PM (13 years, 1 month ago)

The only difference is you get faster colonization, especially with a lot of potent inoculum (the mycelium water) and also, if you clone a really good substrain that fruits really well you'll get better results, usually, then random chance.

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Amazon Shop for: ½ Pint Jars, Agar, Petri Dish

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