Home | Community | Message Board

NorthSpore.com BOOMR Bag!
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: OlympusMyco.com Olympus Myco Grow Bags   North Spore Injection Grain Bag   MagicBag.co All-In-One Bags That Don't Suck   Myyco.com Golden Teacher Liquid Culture For Sale   Left Coast Kratom Buy Kratom Extract   Bridgetown Botanicals CBD Concentrates   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Grow Bags   PhytoExtractum Kratom Powder for Sale

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineIdkleine
Stranger
 User Gallery

Registered: 02/23/14
Posts: 81
Last seen: 2 years, 9 months
Grain spawn question
    #21641998 - 05/05/15 10:37 PM (9 years, 15 days ago)

I was wondering how long is a reasonable time to let my jars of grain spawn sit around between pressure cooking and inoculation? I am using standard mouth quart jars with synthetic filter disks on top. organic rye berries and sterilizing in a A.A. 910 pressure cooker. inoculating from agar petri dishes. Growing pearl and phoenix oyster. using laminar flow hood and basic sterile procedures.

Extras: Filter Print Post Top
InvisibleJuiceh
Dabbing All Day
 User Gallery


Registered: 09/25/12
Posts: 3,208
Re: Grain spawn question [Re: Idkleine]
    #21642330 - 05/05/15 11:53 PM (9 years, 15 days ago)

As long as your sterilization is complete, the jars should be fine for a long time if not indefinitely. The grains on top may dry out a bit. Even though it's not required, I cover my SFD'd lids with foil before pcing and it stays on until inoculation. This seems to help the jars retain the moisture better when they may sit for a while before inoculation. I've left blank jars uninoculated for months. They performed just fine when they were used.

Most people will recommend to use them as soon as they are cool enough after pcing. I don't think that's a bad idea either.


--------------------
Trade List

How I make 15 lbs of casing in a 941. How I line 12 shoeboxes with 1 39gal bag. How I Deep Fry Cubes!
RogerRabbit said:You need a bigger pressure cooker for this hobby. RR

Edited by Juiceh (05/05/15 11:57 PM)

Extras: Filter Print Post Top
OfflineIdkleine
Stranger
 User Gallery

Registered: 02/23/14
Posts: 81
Last seen: 2 years, 9 months
Re: Grain spawn question [Re: Juiceh]
    #21642515 - 05/06/15 12:50 AM (9 years, 15 days ago)

Thx! If I could get away with a week I would be happy. I will cover them and keep them clean. I just needed someone else's opinion on it because I realized I am sterilizing 14 jars of grain, and that's a lot for me to use All at once right now. I have been doing 1 quart of grain spawn to one gallon of sterilized unsuplimented alder sawdust, and then transferring one of those to 30 pasteurized gallon bags of coffee grounds, alder sawdust, and gypsum. All in front of my flow hood. I have done this 3 times (90 bags) and haven't lost any yet.

Extras: Filter Print Post Top
OfflineHumbled
Local Budtender
 User Gallery


Folding@home Statistics
Registered: 01/19/08
Posts: 1,907
Last seen: 2 days, 11 hours
Re: Grain spawn question [Re: Idkleine]
    #21642635 - 05/06/15 01:44 AM (9 years, 15 days ago)

Very nice ldkleine!:awesome: Sounds like you have a good setup and routine going. I usually let the jars cool overnight and try to knock them up as soon as possible. On the other hand, as Juiceh was saying, if you properly PC'd your jars they shouldnt grow anything for quite a while.

I know what we call 'sterilized grain' isn't truly sterile. Just as canned foods will eventually go bad(and those are 'ultra sterilized'), I believe there is a good chance that some dormant endospore will eventually germinate. I could be wrong here:shrug:..... I've never really let jars sit for more than a week before inoculation.

-->To Juiceh, thanks for the info about month old jars. I'm going to give that a try with just a few jars and see how it works out.:thumbup:


--------------------
_-_--->My Trade List<---_-_

Extras: Filter Print Post Top
InvisibleRogerSmith

Registered: 01/29/15
Posts: 365
Re: Grain spawn question [Re: Humbled]
    #21643176 - 05/06/15 07:25 AM (9 years, 14 days ago)

Quote:

Psilocelium said:
On the other hand, as Juiceh was saying, if you properly PC'd your jars they shouldnt grow anything for quite a while.

I know what we call 'sterilized grain' isn't truly sterile. Just as canned foods will eventually go bad(and those are 'ultra sterilized'), I believe there is a good chance that some dormant endospore will eventually germinate.



Exactly what I wanted to point out. What we call sterilization isn't true sterilization. It will contaminate eventually.


I wouldn't hesitate using 3 months old jar. I probably wouldn't use jar older than 6 months.

Extras: Filter Print Post Top
OfflineIdkleine
Stranger
 User Gallery

Registered: 02/23/14
Posts: 81
Last seen: 2 years, 9 months
Re: Grain spawn question [Re: RogerSmith]
    #21644041 - 05/06/15 12:55 PM (9 years, 14 days ago)

Thats great news. Babysitting my pressure cooker takes up way too much time to be only filling it half way. I am running it almost every day, either with grain spawn jars, Agar media, or 2 sawdust filled unicorn bags, which is a huge pain in the ass, getting space between the sides and the bags with a bunch of jar lids. I need a better something something to create that gap, I just haven't figured out what yet. Anyhow its nice to know that I have a good chance of getting away with some time between sterilizing and inoculation. I will always use them as soon as I can of course just to eliminate any possible compromise:)

Extras: Filter Print Post Top
Offlineknomadic_niki
A mile high
 User Gallery


Registered: 06/30/14
Posts: 1,275
Loc: 6200' Colorado
Last seen: 6 years, 9 months
Re: Grain spawn question [Re: Idkleine]
    #21648239 - 05/07/15 08:52 AM (9 years, 13 days ago)

i agree, up to a week is fine. done it myself plenty of times. if you live in a dry climate, leave foil over the lid or something so it doesn't dry out. otherwise, you're good. i wouldn't recommend going over a week.


--------------------
My trade list

In search of sporeless oyster cultures

Extras: Filter Print Post Top
InvisibleRogerSmith

Registered: 01/29/15
Posts: 365
Re: Grain spawn question [Re: knomadic_niki]
    #21648339 - 05/07/15 09:22 AM (9 years, 13 days ago)

Have you ever had a problem using too old jar? Or have you seen old jar contaminate?

But this question depends on many things, type of grains, soaking before sterilization (to get spores to germinate so you can kill them without problem), lenght and temperature of sterilization, storage of sterile jars (fridge), etc.. I should run few test to see how this all works.

Extras: Filter Print Post Top
Offlineknomadic_niki
A mile high
 User Gallery


Registered: 06/30/14
Posts: 1,275
Loc: 6200' Colorado
Last seen: 6 years, 9 months
Re: Grain spawn question [Re: RogerSmith]
    #21648348 - 05/07/15 09:26 AM (9 years, 13 days ago)

i have left "blank" grain spawn jars out for a month without getting contaminated. if you have contamination, you will see it/smell it within a week or so


--------------------
My trade list

In search of sporeless oyster cultures

Extras: Filter Print Post Top
InvisibleJuiceh
Dabbing All Day
 User Gallery


Registered: 09/25/12
Posts: 3,208
Re: Grain spawn question [Re: RogerSmith]
    #21648599 - 05/07/15 10:33 AM (9 years, 13 days ago)

Quote:

RogerSmith said:
Have you ever had a problem using too old jar? Or have you seen old jar contaminate?

But this question depends on many things, type of grains, soaking before sterilization (to get spores to germinate so you can kill them without problem), lenght and temperature of sterilization, storage of sterile jars (fridge), etc.. I should run few test to see how this all works.



I use WBS, sometimes rye berries. Usually soak for 24hrs. Though I have slacked on a couple batches and let them soak up to 3 days. LoL! I try to only do 24hrs or less though. I think extended soaking makes the grains stink more when boiled and PCd, and even after PCing until consumed by mycelium. I pc my grains for a minimum of 90 minutes at 20psi nowadays, used to do 15psi for the same length of time. My last batch I let soak for 3 days. I should leave one of these jars on a shelf for a year to see what happens!


--------------------
Trade List

How I make 15 lbs of casing in a 941. How I line 12 shoeboxes with 1 39gal bag. How I Deep Fry Cubes!
RogerRabbit said:You need a bigger pressure cooker for this hobby. RR

Extras: Filter Print Post Top
InvisibleMudaFuka
Poppin bottles
Male User Gallery


Registered: 12/14/13
Posts: 18,648
Loc: Canada Flag
Trusted Cultivator
Re: Grain spawn question [Re: Juiceh]
    #21648804 - 05/07/15 11:28 AM (9 years, 13 days ago)

I never seem to have enough time to PC everything I need right when I need it. So whenever I find myself with a few spare hours. I will do a PC run. I quite frequently leave sterilised jars un-inoculated for weeks. It has never been a problem. I take the foil off right out of the PC.


--------------------
AMU
Bottle Tek
Liquid Inoculant Tek                                   
                     

Extras: Filter Print Post Top
Offlineforrest
 User Gallery


Registered: 11/16/12
Posts: 1,011
Loc: The Netherlands
Last seen: 4 years, 10 months
Re: Grain spawn question [Re: MudaFuka]
    #21648870 - 05/07/15 11:49 AM (9 years, 13 days ago)

yeah, it feels great to have some sterile petri's or slants with agar in the fridge, ready to inoculate when you get a culture in your mailbox!


--------------------
My Trade List

Extras: Filter Print Post Top
InvisibleMudaFuka
Poppin bottles
Male User Gallery


Registered: 12/14/13
Posts: 18,648
Loc: Canada Flag
Trusted Cultivator
Re: Grain spawn question [Re: forrest]
    #21648969 - 05/07/15 12:17 PM (9 years, 13 days ago)

Yah. I try to keep 20 un-inoculated plates on hand at all times. Preparing them in advance also gives time for any condensation to clear up.


--------------------
AMU
Bottle Tek
Liquid Inoculant Tek                                   
                     

Extras: Filter Print Post Top
OfflineOurNameIsLegion
Stranger

Registered: 11/28/14
Posts: 11
Last seen: 1 year, 5 months
Re: Grain spawn question [Re: MudaFuka]
    #21651691 - 05/08/15 01:27 AM (9 years, 13 days ago)

Good information. I've been keeping mine in the refrigerator until use. Sounds like that's not needed and could just be taking up space. Do you guys think that would have any benefit or disadvantage?

Extras: Filter Print Post Top
InvisibleMudaFuka
Poppin bottles
Male User Gallery


Registered: 12/14/13
Posts: 18,648
Loc: Canada Flag
Trusted Cultivator
Re: Grain spawn question [Re: OurNameIsLegion]
    #21652006 - 05/08/15 05:49 AM (9 years, 12 days ago)

They will dry out faster in the fridge. Fridges are also pretty dirty most of the time.


--------------------
AMU
Bottle Tek
Liquid Inoculant Tek                                   
                     

Extras: Filter Print Post Top
OfflineOurNameIsLegion
Stranger

Registered: 11/28/14
Posts: 11
Last seen: 1 year, 5 months
Re: Grain spawn question [Re: MudaFuka]
    #21653414 - 05/08/15 01:50 PM (9 years, 12 days ago)

Gotcha. Should have clarified it's a mini fridge that's designated for the hobby. Still good to know that they dry out faster. Thanks

Extras: Filter Print Post Top
InvisiblePlantSeeker
Natures Grower
 User Gallery

Registered: 05/02/15
Posts: 118
Re: Grain spawn question [Re: forrest]
    #21662091 - 05/10/15 05:22 PM (9 years, 10 days ago)

=) I need to make a habit out of that, by having media ready to go. I have blue oysters to get going, that I have been to busy to get around to working with.

Extras: Filter Print Post Top
Invisiblehamloaf
Pork Block ®©™√
Male User Gallery

Registered: 12/23/09
Posts: 21,754
Loc: ation undisclosed.
Trusted Cultivator
OG Cultivator
Re: Grain spawn question [Re: OurNameIsLegion]
    #21664122 - 05/11/15 05:27 AM (9 years, 9 days ago)

Quote:

Idkleine said:
Thats great news. Babysitting my pressure cooker takes up way too much time to be only filling it half way. I am running it almost every day, either with grain spawn jars, Agar media, or 2 sawdust filled unicorn bags, which is a huge pain in the ass, getting space between the sides and the bags with a bunch of jar lids. I need a better something something to create that gap, I just haven't figured out what yet. Anyhow its nice to know that I have a good chance of getting away with some time between sterilizing and inoculation. I will always use them as soon as I can of course just to eliminate any possible compromise:)



It's not necessary to place jar lid rings in between bags.  Good practice, but not necessary.  If you are going to skip the jar lid ring step though, and "cram" bags into the PC all you have to do is up your sterilization time about an hour.  The solution to your being limited on how many unicorn bags you can sterilize at once is going to be to get more PC's.  Bigger ones.


--------------------
     
How I Get Stuff done. - My Reference Guide. - My Grows.

Extras: Filter Print Post Top
OfflineRosen_Rot
Learning
I'm a teapot


Registered: 12/06/14
Posts: 1,225
Loc: Goa
Last seen: 1 year, 3 months
Re: Grain spawn question [Re: hamloaf]
    #21668736 - 05/12/15 06:57 AM (9 years, 8 days ago)

I would just like to point out that putting the jars in the PC is not sterilizing, in-fact it was RR that said the whole 90mins is actually an incomplete cycle of sterilization otherwise the grains would turn into mush.

The PC is used to lessen the amount of harmful bacteria leaving a window of opportunity for the mycelium to establish itself and take over

That being said I've left PCs cool down from a few hours (1-2) till about a day. I've read from other cultivators that you can leave jars in your PC up to a week or so without any repercussions.

For fridges I placed my jars in clean ziplock bags, put them in paper bags and store them in the fridge. I've had a couple of master jars in there for a week now, no contamination so far


--------------------
:sporedrop:"The internet has one rule; use or be used" - Bjeldiablo :sporedrop:
''there is no loneliness, only moments where contentment is fleeting''

SBJs "The Basics"
3iRiS9 "Cirque du freak"
B+ BONANZA

   

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: OlympusMyco.com Olympus Myco Grow Bags   North Spore Injection Grain Bag   MagicBag.co All-In-One Bags That Don't Suck   Myyco.com Golden Teacher Liquid Culture For Sale   Left Coast Kratom Buy Kratom Extract   Bridgetown Botanicals CBD Concentrates   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Grow Bags   PhytoExtractum Kratom Powder for Sale


Similar ThreadsPosterViewsRepliesLast post
* spawn question hippy_lettuce 2,066 2 03/21/02 07:33 PM
by hippy_lettuce
* Oops. I guess wood can contaminate. Baby_Hitler 1,475 4 04/14/03 09:54 PM
by falcon
* Question about directly growin to wood kykeon 1,598 8 01/23/03 12:14 AM
by zeronio
* A. blazei mycelium question Mr. Fryd 2,580 7 06/25/01 05:06 PM
by Azure
* A question that needz an answer... please? :) kykeon 2,096 14 12/13/02 04:48 PM
by viscid
* Re: spawn or sporeprint? Anonymous 1,646 1 05/31/00 06:42 PM
by Anonymous
* question about pink oysters Zildjian 2,099 3 10/16/02 06:03 AM
by Zildjian
* Pannelus question r05c03 959 3 03/01/03 01:04 PM
by MushMushi

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, Pastywhyte, Forrester, Stromrider, SHROOMSISAY01
2,249 topic views. 0 members, 5 guests and 3 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.023 seconds spending 0.005 seconds on 12 queries.