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OfflineShamanic Scientist
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Experimental rye tek
    #21629488 - 05/03/15 10:43 AM (9 years, 17 days ago)

Hey guys,

I just want to share something I've been playing with. I have always wanted to get into mushroom cultivation, and started trying in earnest several months ago. I started out with the PF tek and had no real success. I the moved to the standard rye tek and have gotten a few jars to colonize well. With a small stock of jars that I can use for g2g to scale up my operation, I felt comfortable trying out some ideas I had with the hope of getting better/faster growth. It is the standard rye procedure with a couple of extra twists that I'll explain below.


I do a standard overnight soak (in this case, ~15 hours) after rinsing my grain. However, instead of water + dilute coffee + gypsum as I had normally been using, I use dilute coffee + gypsum + "special water." What I do is put Trader Joe's bran flakes into a pot of water and heat it up. This turns the cereal into a kind of goo, which I let cool down some before adding my gypsum, dilute coffee, and rinsed grains:



That's what the heated bran flake goo looks like after heated in water. Below is a picture of the cereal box which gives you an idea for why I wanted to try this. In addition to being made from organic wheat, it's supplemented with things like folic acid niacin, riboflavin, etc. which should be good for cellular growth in general. This stuff is also $1.99/box at Trader Joe's.



After my soak, I simmer my mixture the standard way before steam drying the grain the usual way. So, I now have my soaked grains in a colander together with clumps of gooey bran flakes. I figured the goo means my mixture would be holding more water than it otherwise would. I don't worry about drying the grains as I would for a normal rye tek -- I just let most of the steam come off while moving around the mixture, and then move it into a mixing bowl before adding my next ingredient.

Next, I had this brown rice + quinoa mixture that I get from Costco. Like the bran flakes, this is just something I always have around for myself, but it's not difficult to see why this is something that might be good for encouraging a mycelial orgy.





Ok, so now I did a 90 minute PC, let the jars cool overnight, and inoculated each pint jar with ~2 cc from a multispore syringe. The pictures below are what my jars looked like after just under two weeks. A couple of things to note: my temperature fluctuates somewhat, but is typically between 20-27 degrees celsius, more often toward the lower end. Under my conditions, the growth I see with a normal rye tek is slower than this (it has taken me over a month to get to 100% colonization with standard rye), and the mycelium in these new jars just looks better. At least in these first two test jars, I am seeing more brilliant white, mycorrhizal growth, and I am also seeing growth at many points around the jar. With my normal rye tek jars, I've tended to see growth start from 1-2 points and progress more slowly. Now, I can't cannot rule out genetics as the primary or sole cause of this difference, but this is an encouraging start nonetheless.




I have now shaken these jars, and will update my progress as things proceed. My plan to use some colonized rye jars done the traditional way for g2g into jars that contain my experimental mixture, and ultimately move into monotub using the coir/verm/coffee method.

Edited by Shamanic Scientist (05/03/15 11:10 AM)

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OfflinetaGyo
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Re: Experimental rye tek [Re: Shamanic Scientist]
    #21629499 - 05/03/15 10:47 AM (9 years, 17 days ago)

What do the bran flakes add that the myc want?


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Re: Experimental rye tek [Re: taGyo]
    #21629525 - 05/03/15 10:53 AM (9 years, 17 days ago)

I just added pictures. This is a wheat-based cereal supplemented with a variety of vitamins and minerals. In my mind, things like niacin, riboflavin, folic acid, and B vitamins should encourage growth. These things are used by cells (of any organism) to support functions related to different aspects of DNA replication and cell division. Plus it's an organic wheat product, so it's something mycelium should be good to grow on anyway, just as you could support growth using brown rice, seeds, quinoa, etc. by themselves. I am a novice mushroom cultivator, but using a variety of things that could be sued to support growth on their own should theoretically be a good approach.

Edited by Shamanic Scientist (05/03/15 11:07 AM)

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OfflinetaGyo
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Re: Experimental rye tek [Re: Shamanic Scientist]
    #21629535 - 05/03/15 10:55 AM (9 years, 17 days ago)

I'll be watching,

A good test to see if this benefits at all is to get a clone and do five jars with regular rye tek and five jars with your rye tek.

- Gyo


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Re: Experimental rye tek [Re: Shamanic Scientist]
    #21629547 - 05/03/15 10:57 AM (9 years, 17 days ago)

My thinking for the bran flakes vs. brown rice/quinoa goes like this:

- The bran flake water soak hydrates the grain as it normally would with the standard tek, but the bran flake soup contains many water-soluble nutrients that should go into solution and, hopefully, stick to or get taken up by the grains.

- The brown rice + quinoa mixture has more protein, so I viewed this as primarily a form of amino acid + calorie supplementation. I view the bran flakes as more of a nutrient supplementation.

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OfflinetaGyo
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Re: Experimental rye tek [Re: Shamanic Scientist]
    #21629552 - 05/03/15 10:58 AM (9 years, 17 days ago)

:pipesmoke:


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Re: Experimental rye tek [Re: taGyo]
    #21629557 - 05/03/15 10:58 AM (9 years, 17 days ago)

Yes, this would be the best way to formally test things. The best I can do given my time constraints is to do g2g from the same jar into several jars made from this vs. the regular method.

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Re: Experimental rye tek [Re: Shamanic Scientist]
    #21629560 - 05/03/15 10:59 AM (9 years, 17 days ago)

G2G is a crapshoot from MS.

Your results will vary and be indecisive.


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Re: Experimental rye tek [Re: taGyo]
    #21629579 - 05/03/15 11:03 AM (9 years, 17 days ago)

I understand, but I routinely work ~70 hours/week, so I'm just not sure I will have time in the foreseeable future to expand my skillset beyond what I need to do to start getting a regular fruiting operation going at steady-state. What would cloning involve? Taking a sample from a fruiting body? If so, then I could do a more formal test of this method vs. standard rye tek later in the summer.

Edited by Shamanic Scientist (05/03/15 11:05 AM)

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Re: Experimental rye tek [Re: Shamanic Scientist]
    #21629628 - 05/03/15 11:17 AM (9 years, 17 days ago)

Taking a sample and putting it on agar, yes. Clone from the biggest cluster for higher yield, biggest mushroom for big mushrooms.

Do you use agar?


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Re: Experimental rye tek [Re: taGyo]
    #21629632 - 05/03/15 11:18 AM (9 years, 17 days ago)

I have not used agar before. I won't have time to get into that in the near-term.

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Re: Experimental rye tek [Re: Shamanic Scientist]
    #21629633 - 05/03/15 11:18 AM (9 years, 17 days ago)

It's cool,

We'll wait,

:cool:


--------------------
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TNF Q&A :rockon:
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Dominus fortunae meae sum

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Re: Experimental rye tek [Re: taGyo]
    #21643117 - 05/06/15 06:43 AM (9 years, 14 days ago)

Two days after shake:



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