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hamloaf
Q-dood ®©™√


Registered: 12/23/09
Posts: 24,389
Loc: ation: Based.
Last seen: 1 day, 2 hours
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Welcome To The HLC! (Hamloaf Liquid CULTURE!) 9
#21627998 - 05/02/15 10:13 PM (9 years, 8 months ago) |
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There's a new breed of liquid culture enthusiasts coming forth from their still air boxes, or sterile air laboratories with a new and improved kind of liquid medium. These brave souls will be from here on out known as the "HLC" - Hamloaf Liquid Culture where we're more than just a passing liquid media phase. We are a CULTURE! Haha, whatever. Let's get started.
Collect your end-of-the-boil-cycle grain water into a container that can be frozen, because the end-of-boil-cycle grain water is best preserved/stored frozen. Plastic containers work great. To thaw the frozen, set it into a sauce pan with jar lid rings on the floor of the sauce pan, fill with water, set your stove to medium, and walk away.

Takes about 30-45 minutes to become fully thawed. The end-of-the-boil-cycle grain water may be used freshly after a boil-cycle also. Warning, contents will very be hot when opting to use freshly boiled water, so use caution.
To create mushroom liquid mycelia medium out of the end-of-the-boil-cycle grain water, simply fill your liquid media vessel of choice up with roughly 1 part end-of-the-boil-cycle grain water : 4 parts plain water, cap, sterilize for 45 minutes at 15-17 psi, let cool, inoculate, and BAM! GWLC. Good luck.






Manure/verm sub

Pasteurized wheat straw substrates.


Straw logs.

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shroominmyroom
Grasshopper


Registered: 10/30/14
Posts: 3,639
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf]
#21628008 - 05/02/15 10:15 PM (9 years, 8 months ago) |
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Edited by shroominmyroom (12/11/17 01:57 AM)
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,916
Loc: Milky way
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: shroominmyroom] 2
#21628019 - 05/02/15 10:17 PM (9 years, 8 months ago) |
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completely liquid media only needs to be sterilized 10-15m but if you're real worried 20. why do you use 45.
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jwalt420
Terpin master


Registered: 04/25/11
Posts: 830
Last seen: 2 years, 8 months
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: shroominmyroom]
#21628029 - 05/02/15 10:20 PM (9 years, 8 months ago) |
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hamloaf
Q-dood ®©™√


Registered: 12/23/09
Posts: 24,389
Loc: ation: Based.
Last seen: 1 day, 2 hours
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: bodhisatta] 1
#21628037 - 05/02/15 10:22 PM (9 years, 8 months ago) |
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Quote:
bodhisatta said: completely liquid media only needs to be sterilized 10-15m but if you're real worried 20. why do you use 45.
As an added measure to extra sterility.
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tripdawg420
low life with no life



Registered: 02/02/09
Posts: 7,653
Loc: illinois
Last seen: 6 hours, 13 minutes
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: jwalt420]
#21628045 - 05/02/15 10:24 PM (9 years, 8 months ago) |
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silverstem
Caps & Stems


Registered: 10/12/13
Posts: 900
Loc: jordan
Last seen: 7 years, 5 months
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf]
#21628054 - 05/02/15 10:27 PM (9 years, 8 months ago) |
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I usually dilute mine more... Just a very transparent brown tint is left. But I hardly use gwlc's anymore... Now its just add water to colonized grain jar shake pull water out put it in sterilized jar.....
--------------------
Shroomery needs a gun forum!!!!!!!!! CAN WE HAVE ONE?????
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rxb
n00b-sabot



Registered: 08/24/13
Posts: 15,179
Loc: FREE PSYCHONAUTICA
Last seen: 3 months, 27 days
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: tripdawg420]
#21628067 - 05/02/15 10:30 PM (9 years, 8 months ago) |
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thats a wonderful idea.
i wonder what the shelf life would be if you just sterilized in a jar with the standard equipment lid and did not freeze it.
-------------------- ->$10 FLOW HOOD ALTERNATIVE <-
. i cleaned a mold contaminated live culture and saved it. (might have useful applications)
[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]
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DeighFry
The Last Baron

Registered: 11/26/14
Posts: 200
Loc: Juniper
Last seen: 7 years, 2 months
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: rxb]
#21628549 - 05/03/15 01:23 AM (9 years, 8 months ago) |
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Nice!
I've been waiting for a GWLC TEK that had dilution ratio to it.
What ratio do you use of water to grains to achieve the end-of-boil-cycle water content?
--------------------
Don't fake it baby.
Lay the real thing on me.
Spiraling up through the crack in the skye.
Leaving material world behind.
I see your face in constellations.
The martyr is ending his life for mine.
Edited by DeighFry (05/03/15 03:29 AM)
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ForgottenFreshness
Staying High


Registered: 11/16/13
Posts: 211
Last seen: 1 year, 3 months
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: DeighFry]
#21628559 - 05/03/15 01:27 AM (9 years, 8 months ago) |
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lol nevermind...
Edited by ForgottenFreshness (05/03/15 01:28 AM)
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PsyCLown89
Shroomaloomed



Registered: 08/18/14
Posts: 789
Loc: South Africa
Last seen: 3 years, 25 days
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Nice! I saved some grain water a few months back and planned to make some LC with it (sorry, HLC now :P).
I did not freeze them or sterilize them (planned to later on), forgot about them and after a week had nice stuff floating on top and looked super manky. Same happened with some jars of hydrated sorghum, very quickly grew some stuff which was not mycelium 
Perhaps sometime I will get a chance to actually try it!
-------------------- My Trade List - Click ME!! [Updated: 02/05/2015]
"It's all in your head"
"Haste makes waste!"
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hamloaf
Q-dood ®©™√


Registered: 12/23/09
Posts: 24,389
Loc: ation: Based.
Last seen: 1 day, 2 hours
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: DeighFry]
#21628787 - 05/03/15 04:17 AM (9 years, 8 months ago) |
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Quote:
DeighFry said: Nice!
I've been waiting for a GWLC TEK that had dilution ratio to it.
What ratio do you use of water to grains to achieve the end-of-boil-cycle water content?
Nah, nothing too precise like that. Just go about your normal grain prep technique, and instead of throwing away that end-of-the-boil-cycle grain water, collect it, and use it as your liquid media of choice. Fast. Easy. Extremely effective.
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf] 1
#21628857 - 05/03/15 05:27 AM (9 years, 8 months ago) |
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like the gallon jug idea dude!! LC probably best way to do those, i picked up two on recycling day recently from the curb, and just been staring at them, imagining how to use them. as always, rock on with ur bad self brother
-------------------- A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!
The whole is greater than the sum of its parts
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DeighFry
The Last Baron

Registered: 11/26/14
Posts: 200
Loc: Juniper
Last seen: 7 years, 2 months
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf]
#21629761 - 05/03/15 11:49 AM (9 years, 8 months ago) |
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Quote:
hamloaf said:
Quote:
DeighFry said: Nice!
I've been waiting for a GWLC TEK that had dilution ratio to it.
What ratio do you use of water to grains to achieve the end-of-boil-cycle water content?
Nah, nothing too precise like that. Just go about your normal grain prep technique, and instead of throwing away that end-of-the-boil-cycle grain water, collect it, and use it as your liquid media of choice. Fast. Easy. Extremely effective.
Right on.
--------------------
Don't fake it baby.
Lay the real thing on me.
Spiraling up through the crack in the skye.
Leaving material world behind.
I see your face in constellations.
The martyr is ending his life for mine.
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hamloaf
Q-dood ®©™√


Registered: 12/23/09
Posts: 24,389
Loc: ation: Based.
Last seen: 1 day, 2 hours
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: PsyCLown89]
#21629944 - 05/03/15 12:28 PM (9 years, 8 months ago) |
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Quote:
PsyCLown89 said: Nice! I saved some grain water a few months back and planned to make some LC with it (sorry, HLC now :P).
I did not freeze them or sterilize them (planned to later on), forgot about them and after a week had nice stuff floating on top and looked super manky. Same happened with some jars of hydrated sorghum, very quickly grew some stuff which was not mycelium 
Perhaps sometime I will get a chance to actually try it!
Yes. Not freezing your end-of-the-boil-cycle grain water for purpose of long term storage will lead to contamination faster than lightening. Same thing with capped jars of hydrated, unpasteurized casing, and/or bulk substrate materials. Enclosing casing, and/or hydrated bulk sub materials creates anaerobic environments in which bacteria, and mold breed faster than bunnies. No need to hydrate your casing, and/or sub materials up until just before time to pasteurize. Once casing, and/or sub materials are pasteurized it's best to use the materials as soon as they cool to room temps, so plan and schedule accordingly. Good luck!
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NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf] 1
#21629974 - 05/03/15 12:36 PM (9 years, 8 months ago) |
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Wow. I think I'm finally gonna try this. I've been wanting to take another stab at LC and this looks very easy.
   
--------------------
Let it grow! Let it grow! Greatly yield!
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in
DOG FOOD AGAR
MY ELECTRIC INOCULATION LOOP
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Buck513

Registered: 04/17/14
Posts: 5,682
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: NumeroEno]
#21630122 - 05/03/15 01:18 PM (9 years, 8 months ago) |
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Nice write up mane. I might try this eventually
-------------------- Fail to plan and you plan to fail.
Enter the Ban Lottery
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blackout


Registered: 07/16/00
Posts: 5,266
Last seen: 1 year, 2 months
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: Buck513] 2
#21630156 - 05/03/15 01:29 PM (9 years, 8 months ago) |
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You could fill those ice cube bags with liquid and take them out as you need them. Defrosting in a microwave would be faster.
I prefer clear LCs and was wondering about using wine/beer finings (clearing agents). I have some grain water at the moment, and as with beer I found it cleared a lot faster in the fridge, and of course would be less prone to contams if settling in the fridge than just sitting in your room.
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hamloaf
Q-dood ®©™√


Registered: 12/23/09
Posts: 24,389
Loc: ation: Based.
Last seen: 1 day, 2 hours
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: blackout]
#21630347 - 05/03/15 02:15 PM (9 years, 8 months ago) |
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Trying of some wine/beer finings as a liquid media choice would be advocated, and encouraged, blackout. Good idea. Your findings posted would be appreciated, as well. 
A bit partial to the golden liquid mediums, personally, due to coming up on trying out Karo, SITR, DEX/LME, and straight LME with straight LME being most powerful liquid media broth generated consecutively. GWLC trumps ALL aforementioned liquid media recipes. Hypothesis as to why grain water liquid cultures do better than their predecessors, consecutively, lies within mycelia being already used to eating, and digesting grain medium before LC ever hits grain.
As far as storage of end-of-the-boil-cycle grain water, yes, storing media in refrigerator would be better plan, than storing media at room temps on a shelf. However, after media is stored in fridge for a long period of time EVENTUALLY contams are going to bloom even in lower temperature ranges of inside refrigerator. For long term storage of end-of-the-boil-cycle grain water it's best to be stored in freezer. Storing end-of-the-boil-cycle grain water in freezer stops whole range of biochemical processes, thus, no life can grow when the media is frozen.
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DeighFry
The Last Baron

Registered: 11/26/14
Posts: 200
Loc: Juniper
Last seen: 7 years, 2 months
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf]
#21630801 - 05/03/15 04:24 PM (9 years, 8 months ago) |
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Quote:
hamloaf said: However, after media is stored in fridge for a long period of time EVENTUALLY contams are going to bloom even in lower temperature ranges of inside refrigerator. For long term storage of end-of-the-boil-cycle grain water it's best to be stored in freezer. Storing end-of-the-boil-cycle grain water in freezer stops whole range of biochemical processes, thus, no life can grow when the media is frozen.
I tried to store rye grass soak and rye soak water in the fridge recently. It took maybe about a week to notice spoilage.
--------------------
Don't fake it baby.
Lay the real thing on me.
Spiraling up through the crack in the skye.
Leaving material world behind.
I see your face in constellations.
The martyr is ending his life for mine.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,972
Loc: Canada
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: DeighFry] 1
#21631148 - 05/03/15 06:04 PM (9 years, 8 months ago) |
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Nice writeup
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invitro

Registered: 05/03/13
Posts: 2,529
Last seen: 1 year, 1 month
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf]
#21631374 - 05/03/15 07:02 PM (9 years, 8 months ago) |
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NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: Pastywhyte] 1
#21631375 - 05/03/15 07:02 PM (9 years, 8 months ago) |
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Well, it's official. I'm soaking some rye and I'm gonna knock up some of the water with GT and blue oyster. I can't wait! LC is the one thing that has eluded me since I joined the site and I think I'll finally be able to pull it off this way!
--------------------
Let it grow! Let it grow! Greatly yield!
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in
DOG FOOD AGAR
MY ELECTRIC INOCULATION LOOP
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shroominmyroom
Grasshopper


Registered: 10/30/14
Posts: 3,639
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: NumeroEno] 2
#21631476 - 05/03/15 07:28 PM (9 years, 8 months ago) |
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Edited by shroominmyroom (12/11/17 01:59 AM)
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Psychonautica
Cuddly Wuddly Fuccboi


Registered: 04/20/15
Posts: 10,854
Loc: Free Soul & IISkuNkII
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: NumeroEno]
#21631487 - 05/03/15 07:31 PM (9 years, 8 months ago) |
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Okay, so, forgive me for going full retard for a second here, or at least partial retard..
This nonsense about your "end-of-boil-cycle-water" is, the water that's left over, after you boil your grains right, before sterilizing them? Just making sure I understand what's going on here. I'm quite dense sometimes.
-------------------- The chances of you even being born, Were forty million to one. There's two parts of the statistic And I want you to live through one
3/8/95 - 7/10/15 Rest In Paradise, Brother.
Sheekle said:
yeah, i said i was afraid of psychonautica

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rxb
n00b-sabot



Registered: 08/24/13
Posts: 15,179
Loc: FREE PSYCHONAUTICA
Last seen: 3 months, 27 days
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: Psychonautica] 1
#21631607 - 05/03/15 07:57 PM (9 years, 8 months ago) |
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correct
-------------------- ->$10 FLOW HOOD ALTERNATIVE <-
. i cleaned a mold contaminated live culture and saved it. (might have useful applications)
[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]
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DeighFry
The Last Baron

Registered: 11/26/14
Posts: 200
Loc: Juniper
Last seen: 7 years, 2 months
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: Psychonautica]
#21631748 - 05/03/15 08:25 PM (9 years, 8 months ago) |
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I'm sure he'll (hamloaf) answer, but for the sake of a speedy reply, yes.
--------------------
Don't fake it baby.
Lay the real thing on me.
Spiraling up through the crack in the skye.
Leaving material world behind.
I see your face in constellations.
The martyr is ending his life for mine.
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Psychonautica
Cuddly Wuddly Fuccboi


Registered: 04/20/15
Posts: 10,854
Loc: Free Soul & IISkuNkII
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: DeighFry]
#21631800 - 05/03/15 08:34 PM (9 years, 8 months ago) |
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Quote:
DeighFry said: I'm sure he'll (hamloaf) answer, but for the sake of a speedy reply, yes.
There's no need for me to waste the Great Loaf's time with my sillyness, I appreciate the speedy answers, I would have trusted rxb, because that's the idea I was drawing from this whole thread, but now that two people have concurred I'm happy with accepting it.
Thanks!
-------------------- The chances of you even being born, Were forty million to one. There's two parts of the statistic And I want you to live through one
3/8/95 - 7/10/15 Rest In Paradise, Brother.
Sheekle said:
yeah, i said i was afraid of psychonautica

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Bigbadwooof
Trump's Bone Spurrs



Registered: 12/07/13
Posts: 16,298
Last seen: 11 hours, 6 minutes
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: Psychonautica]
#21632054 - 05/03/15 09:23 PM (9 years, 8 months ago) |
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I like it! Waste not, want not, and what not!
-------------------- "It is no measure of good health to be well adjusted to a profoundly sick society," - Jiddu Krishnamurti
FARTS
"No my dog won't bite you, tho it had the right to. You ought to give him credit, cuz he knows I woulda let him" - Modest Mouse (And Wooof!)
Every one of you should see this video.
"Facts are chiels that winna ding, and downa be disputed" - Robert Burns
 
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NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: Psychonautica]
#21632059 - 05/03/15 09:23 PM (9 years, 8 months ago) |
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I just soaked some grains and reserved the water. Gonna PC 2 regular mouth half pints of this stuff. Got some nice clean extra agar plates to inoculate them with.
--------------------
Let it grow! Let it grow! Greatly yield!
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in
DOG FOOD AGAR
MY ELECTRIC INOCULATION LOOP
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hamloaf
Q-dood ®©™√


Registered: 12/23/09
Posts: 24,389
Loc: ation: Based.
Last seen: 1 day, 2 hours
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: NumeroEno]
#21632168 - 05/03/15 09:42 PM (9 years, 8 months ago) |
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Quote:
NumeroEno said: Well, it's official. I'm soaking some rye and I'm gonna knock up some of the water with GT and blue oyster. I can't wait! LC is the one thing that has eluded me since I joined the site and I think I'll finally be able to pull it off this way!
Quote:
NumeroEno said: I just soaked some grains and reserved the water. Gonna PC 2 regular mouth half pints of this stuff. Got some nice clean extra agar plates to inoculate them with.
Awesome, NumeroEno. Very cool. Let us know how progress is going as developments are made.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,972
Loc: Canada
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: NumeroEno]
#21632170 - 05/03/15 09:42 PM (9 years, 8 months ago) |
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I am pleased with the positive responses here. Was only a few years ago that I was afraid to tell people i used LC, let alone what it was made of. Good stuff people. Remember grain water works fantastic for agar as well
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shroominmyroom
Grasshopper


Registered: 10/30/14
Posts: 3,639
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: Pastywhyte]
#21632200 - 05/03/15 09:50 PM (9 years, 8 months ago) |
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Edited by shroominmyroom (12/11/17 02:00 AM)
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hamloaf
Q-dood ®©™√


Registered: 12/23/09
Posts: 24,389
Loc: ation: Based.
Last seen: 1 day, 2 hours
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: shroominmyroom]
#21633769 - 05/04/15 08:54 AM (9 years, 8 months ago) |
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Ganoderma lucidum - Red Reishi whole oat HLC inoculated with LC from vendor.

Can you guess what the fate of this culture's going to be?
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pablokabute
Hari ng Amag



Registered: 11/22/11
Posts: 5,243
Loc: rural ghetto
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf]
#21636675 - 05/04/15 08:08 PM (9 years, 8 months ago) |
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Posting for reference. Thanks a lot ham.

--------------------
Fermented Mushrooms!!
--- https://www.shroomery.org/forums/showflat.php/Number/23378638/fpart/1/vc/1
'The second seal: “All CONTAMINATED things and events are unsatisfactory.”'
"I envy you. You North Americans are very lucky. You are fighting the most important fight of all - you live in THE HEART OF THE BEAST."
--Anonymous Guerilla, or is he..
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: pablokabute] 1
#21638261 - 05/05/15 05:41 AM (9 years, 8 months ago) |
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going to take some updated pictures on my hamloaf liquid cultures soon. also i make so much grain that i have so much boil water leftover after grain prep. that i don't even have to store it frozen. i can simply make my next batch of grain and prep my lc at the same time. its great! extremely easy as well.
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NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: eatyualive] 1
#21638742 - 05/05/15 09:33 AM (9 years, 8 months ago) |
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I knocked up 2 tall half pints of this grain water LC yesterday. AA+ and blue oyster. I really hope they come out clean, so I can finally say I made a successful LC.
--------------------
Let it grow! Let it grow! Greatly yield!
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in
DOG FOOD AGAR
MY ELECTRIC INOCULATION LOOP
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CplCauliflower
shroomyboi


Registered: 02/21/15
Posts: 135
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf]
#21639070 - 05/05/15 11:02 AM (9 years, 8 months ago) |
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Bump
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Jeff Vader
Ineffable



Registered: 08/08/14
Posts: 427
Loc: South Africa
Last seen: 2 years, 6 months
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: CplCauliflower]
#21639209 - 05/05/15 11:51 AM (9 years, 8 months ago) |
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Been considering doing this for a while now. I tend to over PC the honey LC. No such issue's with this tek.
--------------------
“With four hundred milligrams of moksha-medicine in their bloodstreams, even beginners
can catch a glimpse of the world as it looks to someone who has been liberated
from his bondage to the ego.”
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DeighFry
The Last Baron

Registered: 11/26/14
Posts: 200
Loc: Juniper
Last seen: 7 years, 2 months
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: Jeff Vader]
#21639433 - 05/05/15 01:13 PM (9 years, 8 months ago) |
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Quote:
Jeff Vader said: I tend to over PC the honey LC. No such issue's with this tek.
Same here man.
--------------------
Don't fake it baby.
Lay the real thing on me.
Spiraling up through the crack in the skye.
Leaving material world behind.
I see your face in constellations.
The martyr is ending his life for mine.
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blackout


Registered: 07/16/00
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf]
#21639471 - 05/05/15 01:29 PM (9 years, 8 months ago) |
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Quote:
hamloaf said: Trying of some wine/beer finings as a liquid media choice would be advocated, and encouraged, blackout. Good idea.
egg whites can be used to clear wine & beer so I guess it would work with these LCs, you would only need a small bit of egg white for the typical volume of LC we use.
And yes freezing is no doubt the best storage method long term. If people are letting it try and settle to decant it off the fridge is the best bet, as it will not only retard growth, but clear it faster too. I often cap unfiltered jars and put them in a basin of water to cool faster too. You do not want them sitting at typical incubation temps for any length of time.
I was reading on mycoptia that some guy apparently was doing a course in aloha medicinals and apparently they do not boil grains in excess water as they believe straining water off gets rid of water soluble nutrients. They do wash them before processing though. I usually wash mine with washing up liquid and rinse it off. Maybe these water soluble nutrients are what makes these grain LCs so good.
There will also be some naturally occuring sugars in the grain, and I know Workman favours very dilute sugar based LCs, only 0.1-0.5% sugars.
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eatyualive
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: blackout]
#21640225 - 05/05/15 04:47 PM (9 years, 8 months ago) |
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would you mind updating the op with your stir plate spinning information? example, how long and how many rpms..
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eatyualive
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Registered: 08/17/01
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: shroominmyroom] 1
#21640230 - 05/05/15 04:47 PM (9 years, 8 months ago) |
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Quote:
shroominmyroom said:

GWLC is awesome.

Panaeolus Bisporus, seven days since HLC inoculation
nice pics! its almost like a variety of good beer!
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Dhearic


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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: eatyualive]
#21640249 - 05/05/15 04:52 PM (9 years, 8 months ago) |
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Quote:
eatyualive said: would you mind updating the op with your stir plate spinning information? example, how long and how many rpms..
This would be very helpful info. I'm gonna use this tek at my next go with LCs, normally I've used either Karo or Honey.
Thanks for the good write up Loaf
--------------------
Credit where credit is due.
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hamloaf
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: Dhearic]
#21643451 - 05/06/15 09:34 AM (9 years, 8 months ago) |
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Went a little hard this morning, and decided to do 1:2 (half strength) grain water to plain water ratio HLC.


 Unsterilized end-of-boil-cycle grain water comprised of rye/horse maure/gypsum/spent coffee grounds.
The two quart jar mediums will be inoculated with cube mycelium. LC generated inside of the large, quart jars will be used to inoculate sterilized bulk subs with hydrated grains inside of spawn bags using LC gun @ 30-40cc's of LC per bag. The pint sized media will most likely be inoculated with a pre-meditated/random edible wedge generated from LC from vendor, and it's content given away in the market place. Also, rubber gaskets on each of these new liquid mediums were utilized.
No leaks!

These mediums are in the AA x25 warming up to boiling temps, and are scheduled to be PC'ed for an hour and a half at 15-17 psi.
Edited by hamloaf (05/06/15 10:00 AM)
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Dhearic


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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf]
#21643577 - 05/06/15 10:21 AM (9 years, 8 months ago) |
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You wouldn't happen to know where to buy those high quality pp5 plastic lids in the wide mouth(90mm) size would you? I bought 10 of the higher quality regular mouth lids to use with LCs and LIs but after hours of searching I've never found a wide mouth equivalent.
I like the construction of the solid regular mouth lids way better than the flimsy shit from wally world that they have for wide mouths.
Using up the last of my jars that I topped with metal lids today. Nothing but plastic for this guy from here on out So many torn gloves from trying to wrench open old metal lids....
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Credit where credit is due.
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DeighFry
The Last Baron

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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: Dhearic]
#21643720 - 05/06/15 11:07 AM (9 years, 8 months ago) |
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@Dhearic
I get all of my plastic wide mouth lids from Wal-Mart & Fred Meyers.
I was doing SAB work, and tried to wrench the pressure-sealed metal lid off.You gotta' wrench those muddas, when the pressure gave, my arm flew up and threw my whole SAB. A whole session, and 3 plates laid to waste.
Plastics are better, I agree.
--------------------
Don't fake it baby.
Lay the real thing on me.
Spiraling up through the crack in the skye.
Leaving material world behind.
I see your face in constellations.
The martyr is ending his life for mine.
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blackdust


Registered: 02/28/09
Posts: 8,327
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf]
#21643766 - 05/06/15 11:19 AM (9 years, 8 months ago) |
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this is the type of stuff i make PDF of the page and add them to my notes
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rxb
n00b-sabot



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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: blackdust]
#21645944 - 05/06/15 08:20 PM (9 years, 8 months ago) |
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i wonder if adding sugar/honey/dme etc to the boil phase would help give the myc something to fee on, and increase usefulness of using the boil liquid as a lc media.
-------------------- ->$10 FLOW HOOD ALTERNATIVE <-
. i cleaned a mold contaminated live culture and saved it. (might have useful applications)
[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]
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CplCauliflower
shroomyboi


Registered: 02/21/15
Posts: 135
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: rxb]
#21646733 - 05/06/15 10:48 PM (9 years, 8 months ago) |
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Super interesting shit here. Can't wait to get my May grow log going.
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hamloaf
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: Dhearic]
#21646846 - 05/06/15 11:15 PM (9 years, 8 months ago) |
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Quote:
Dhearic said: You wouldn't happen to know where to buy those high quality pp5 plastic lids in the wide mouth(90mm) size would you? I bought 10 of the higher quality regular mouth lids to use with LCs and LIs but after hours of searching I've never found a wide mouth equivalent.
I like the construction of the solid regular mouth lids way better than the flimsy shit from wally world that they have for wide mouths.
Using up the last of my jars that I topped with metal lids today. Nothing but plastic for this guy from here on out So many torn gloves from trying to wrench open old metal lids....
Nah, I haven't. If you do let us know, please, and the same will be done on the part of this cultivator.
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rxb
n00b-sabot



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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf]
#21646857 - 05/06/15 11:18 PM (9 years, 8 months ago) |
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-------------------- ->$10 FLOW HOOD ALTERNATIVE <-
. i cleaned a mold contaminated live culture and saved it. (might have useful applications)
[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]
Edited by rxb (05/06/15 11:19 PM)
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hamloaf
Q-dood ®©™√


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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: rxb]
#21646870 - 05/06/15 11:21 PM (9 years, 8 months ago) |
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Quote:
rxb said: i wonder if adding sugar/honey/dme etc to the boil phase would help give the myc something to fee on, and increase usefulness of using the boil liquid as a lc media.
Doubt this. Sugar LC's are found to be inferior to straight LME LC's. LME LC's become inferior once DEX is added, so I think the same would ring true for the HLC. If you want additives in your HLC, try throwing shit like manure, gypsum, wheat bran, wheat straw, spent coffee grinds, ect into your boil water at the beginning of the soaking cycle.
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rxb
n00b-sabot



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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf]
#21646888 - 05/06/15 11:26 PM (9 years, 8 months ago) |
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i do gypsum and coffee spent/not doesnt seem to effect it.
never added much else.
-------------------- ->$10 FLOW HOOD ALTERNATIVE <-
. i cleaned a mold contaminated live culture and saved it. (might have useful applications)
[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]
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hamloaf
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: rxb] 2
#21646911 - 05/06/15 11:32 PM (9 years, 8 months ago) |
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Ended up getting those three mediums that were generated earlier out of the half strength rye/horse manure/gypsum/spent coffee gounds HLC sterilized, and inoculated all in one day. The pint sized medium was inoculated with Grifola Frondosa - Maitake Mushroom agar wedge. The two quart jar mediums were inoculated with Psilocybe Cubensis agar wedge. 1 jar, Pacifica Exotica Spora Hawaiian (PESH), and the other quart jar, Golden Teacher.
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eatyualive
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf] 1
#21650160 - 05/07/15 06:28 PM (9 years, 8 months ago) |
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here is my hamloaf grain water liquid culture. its a few days in. ive put it to the stir plate twice for about 1 minute bursts on each jar. this was just after the spin.

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hamloaf
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: eatyualive]
#21650244 - 05/07/15 06:51 PM (9 years, 8 months ago) |
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Awesome! Indeedsir. Recently I have been stirring my LC's every time the lab is entered which is every 1-2 days. Pint sized LC's each have an inch long stirbar in them, and have been getting stirred at 700-800rpms for 5-10 minutes at a time. The reason for the high rpm stir on the pint sized jars is due to the utilization of the 1 inch stirbar. The 1 inch stirbar needs higher rpms to really break up that culture, so from here on out, the 1 inch stir bar will be reserved for species with fragile mycelium. With hardier myceliumed species the industry standard stir bar is going to be either the 2 inch stir bars, or the 3.5cm circular stirbar (the shit).

The quart jar LC master that's spread between three threads (WBS HLC) has the 3.5 circular stir bar in it. The circular stir bar shreds and breaks apart the liquid myce at a much lower rpms than the 1 inch stir bar. When stirring culture within the quart jar with 3.5cm stir bar, 500-555rpms is used for roughly 5-10 minutes. A shift in which stir bar to use, and in what sized vessel has been made. In the new HLC's - 2 inch magnetic stir bars were used in the quart jar, and one of the 3.5 circular stir bars were used in the pint sized jar. We'll see how this set up works out before deciding to settle on any one method/set up yet.
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf]
#21650292 - 05/07/15 07:03 PM (9 years, 8 months ago) |
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i only have one small stir bar. and the other is the long one. so i used what i had. i get quite the tornado going on the larger stir bar in a pint jar. i only need it to spin for a minute and it works great. i do about the same your doing 1-2 days then stir! once the lc's grow a little more ill up the time for sure. thanks for the info.
on my next lc's im going to go with the darker more concentrated lc. i love the bold color.
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hamloaf
Q-dood ®©™√


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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: eatyualive] 1
#21651725 - 05/08/15 01:46 AM (9 years, 8 months ago) |
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You will not be disappointing by making a HLC less diluted. The HLC is STRONGER the less you dilute it with water. Downside : the less the medium is diluted the murkier/not see through the LC will be. Depending on who you are, this could be a PITA. Try telling that to the mushroom mycelium though. 
Check this out all you myco-voyeurs out there. Plates of agar poured using the grain water culturing media generation technique. Media used : 500ml's of half strength end-of-boil-cycle grain water rye/horse manure/gypsum/spent coffee grounds mixed with 10 grams of "Now" brand, grocery store agar agar sterilized at 17-20 psi for 45 minutes.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,972
Loc: Canada
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf]
#21651953 - 05/08/15 04:57 AM (9 years, 8 months ago) |
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Nice, I love that recipe
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DeighFry
The Last Baron

Registered: 11/26/14
Posts: 200
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf]
#21653113 - 05/08/15 12:26 PM (9 years, 8 months ago) |
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Quote:
hamloaf said: "Now" brand, grocery store agar agar
What grocery store do you find agar Ham? I've had no luck.
--------------------
Don't fake it baby.
Lay the real thing on me.
Spiraling up through the crack in the skye.
Leaving material world behind.
I see your face in constellations.
The martyr is ending his life for mine.
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rxb
n00b-sabot



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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: DeighFry]
#21653126 - 05/08/15 12:31 PM (9 years, 8 months ago) |
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depends on where you are as to where you find agar.
in alot of cities its in kroger (not mine).
in alot of cities its aparently in walmart says the internets (not mine)
in mine they have it at the vegetarian grocery store, at whole foods, and at the health food store.
-------------------- ->$10 FLOW HOOD ALTERNATIVE <-
. i cleaned a mold contaminated live culture and saved it. (might have useful applications)
[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]
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DeighFry
The Last Baron

Registered: 11/26/14
Posts: 200
Loc: Juniper
Last seen: 7 years, 2 months
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: rxb]
#21653233 - 05/08/15 12:53 PM (9 years, 8 months ago) |
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Quote:
rxb said: in mine they have it at the vegetarian grocery store, at whole foods, and at the health food store.
I'll have to try more of those then. I tried GNC, no luck.
Thanks.
--------------------
Don't fake it baby.
Lay the real thing on me.
Spiraling up through the crack in the skye.
Leaving material world behind.
I see your face in constellations.
The martyr is ending his life for mine.
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rxb
n00b-sabot



Registered: 08/24/13
Posts: 15,179
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: DeighFry]
#21653361 - 05/08/15 01:32 PM (9 years, 8 months ago) |
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yeah i dont mean like gnc.
i mean in stores where they sell everything organic and fair trade but food.
and
in stores where there are no meat products.
hindu groceries.
-------------------- ->$10 FLOW HOOD ALTERNATIVE <-
. i cleaned a mold contaminated live culture and saved it. (might have useful applications)
[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]
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DeighFry
The Last Baron

Registered: 11/26/14
Posts: 200
Loc: Juniper
Last seen: 7 years, 2 months
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: rxb]
#21653396 - 05/08/15 01:44 PM (9 years, 8 months ago) |
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A hunting I will go.
--------------------
Don't fake it baby.
Lay the real thing on me.
Spiraling up through the crack in the skye.
Leaving material world behind.
I see your face in constellations.
The martyr is ending his life for mine.
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invitro

Registered: 05/03/13
Posts: 2,529
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf]
#21653648 - 05/08/15 02:48 PM (9 years, 8 months ago) |
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What is meant by "stronger" ?
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: invitro] 1
#21654823 - 05/08/15 08:10 PM (9 years, 8 months ago) |
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im going to give that a try very soon! thanks for the pointer.
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hamloaf
Q-dood ®©™√


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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: DeighFry]
#21656243 - 05/09/15 07:41 AM (9 years, 8 months ago) |
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Quote:
DeighFry said:
Quote:
hamloaf said: "Now" brand, grocery store agar agar
What grocery store do you find agar Ham? I've had no luck.
"Now" brand agar powder is personally sourced from a local Ma & Pa heath food/organic grocery store. Asian markets have "whole" agar that needs to be ground up. Agar is a seaweed.
Here are a few links for online sources of "Now" brand agar agar. http://www.vitaglo.com/6410.html http://www.nowfoods.com/Agar-Powder-2oz.htm http://www.amazon.com/Foods-Agar-Powder-Ounce-Bottle/dp/B000MGSJ5Ahttp://www.amazon.com/Foods-Agar-Powder-Ounce-Bottle/dp/B0017WEA6K
This is the way to go if you aren't going to use grain water as your nutrient source for agar media. http://www.fungi.com/search-results.html#/?q=agar
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hamloaf
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: invitro]
#21656264 - 05/09/15 07:53 AM (9 years, 8 months ago) |
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Quote:
invitro said: What is meant by "stronger" ?
The less you dilute a HLC, at the expense of generating a media that is difficult to impossible to see through, the more luxurious, vibrant, and aggressive the mushroom mycelium becomes on the grain substrate, and I have a few theories as to why. It's understood the role of genetics when it comes to colonization speed, and rigor. It's also understood that media, and growing conditions/environment ALSO play a key roll in speed, and vigor of colonization. That being said, beefing up your HLC with a higher concentration of grain water/nutrient source is a factor of gaining a superior culture due to the type/style of media used.
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Pastywhyte
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf] 1
#21656455 - 05/09/15 09:01 AM (9 years, 8 months ago) |
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The reason I started to play with grain water media was because i was so dissatisfied with the performance of simple sugar LCs. The culture was always weak in terms of recovery after inoculation of grain spawn and the growth within the LC was usually sparse as well. Despite everything that I read telling me that 2% nutes was enough for LC and that simple sugars were fine as a nute source I was frustrated with it every time. Even malt extract (which was a slight improvement over most sugar LC's) was not enough to really make me feel like it was achieving what it should have been.
My first grain water LC was from rye and I was blown away at not only how much faster the culture recovered after inoculation, but also at how thick and aggressive the growth was. I suspected that the rich nutrient state of the inoculating media contributed to the culture becoming aggressive when it was presented with less accessable media in the form of grain.
The kicker is all that nutrition does mean that more care needs to be taken as the soak water is loaded with endospores and in such an environment they can bloom fast. I also find that massive temperature swings during colonization can be detrimental to the grain water LC's health as well. So a longer sterilization cycle and care in storage is helpful. I actually usually sterilize my grain water LC with my grains. That would totally carmelize a sugar LC but its less of a problem for the grain water.
Edited by Pastywhyte (05/09/15 09:09 AM)
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Tomandjerry58
Stranger

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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: Pastywhyte]
#21656510 - 05/09/15 09:18 AM (9 years, 8 months ago) |
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Have you guys ever seen anything like this in an lc. Pan cam in dextrose/lme. Kind of looks like fruits and they float
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hamloaf
Q-dood ®©™√


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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: Tomandjerry58]
#21656580 - 05/09/15 09:41 AM (9 years, 8 months ago) |
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Whoa, what the heck do you have going on there? Can you get a topical view of the phenomenon please? Also, what's up with all those little circular colonies of mycelium all over? That's not a good sign. Neither is your floating phenomenon as far as can be told from here, currently. Never seen anything like the floating before, no. Are you using magnetic stirring devices, or are you stirring by hand?? Definitely test that culture on a small sterilzed medium such as a plate of agar, pf cake, or a pint jar of grains before using that LC for mass inoculations.
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directexperience
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf]
#21656604 - 05/09/15 09:50 AM (9 years, 8 months ago) |
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This is really a wonderful money saving idea
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Tomandjerry58
Stranger

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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf]
#21656764 - 05/09/15 10:39 AM (9 years, 8 months ago) |
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Inoculated with a very small wedge and hand stirred every couple of days. The little colonies were seen early on latching onto to the very small undissolved sugar it seemed (I did not filter) .I pulled a syringe out of it and it was viable, then it was left for like 3 weeks with no stirring at all.
I decided to look at it today because my outdoor patch is starting to grow out. But it didn't have these lil growth,floaty things before. When time allows,I will test it but I have a lot of doubt at this point lol
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PlantSeeker
Natures Grower


Registered: 05/02/15
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf]
#21662015 - 05/10/15 05:02 PM (9 years, 8 months ago) |
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Keep up the good work. Love reading your posts!
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eatyualive
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: PlantSeeker] 1
#21684753 - 05/15/15 09:01 PM (9 years, 8 months ago) |
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day 2 after inoculation signs of leap off! now, the LI i did has colonized quite a bit in 4 days. but i used 4 times the amount of LI than LC here. the LC is showing leap off a day faster.
i went extremely light with only 1 cc per quart as i need to delay these jars about a week. all jars are showing leap off. the updated image will more than likely be fully colonized by the time i can check it.

i also used my nice utensil here. i wrapped tip in foil. making sure that the part that opens up is the part that screws on to the syringe body at the bottom of the picture below. i pressure cooked for 20 mins at 15psi. once cool, setup a sab, put all utensils, GWLC, Grain Jars and sterile syringe body still wrapped in the plastic inside the sab. i halfway unwrap the syringe body plastic not exposing the tip yet. i unfold the foil just enough so that when i pull the syringe body out, i immediately place the syringe plastic needle on the body. the rest of the foil is covering the syringe tip until the liquid is to be taken. i lifted the lid of the lc, sucked up the lc. closed the lid. lifted lid of receiving jar, squirted 1cc, then closed the lid. this was one steady motion in a sab. i didn't touch the syringe tip on anything and i did this for 15 jars in a row. I also don't want to melt these syringe tips.
syringe needle
Edited by eatyualive (05/15/15 09:16 PM)
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hamloaf
Q-dood ®©™√



Registered: 12/23/09
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: eatyualive]
#21685319 - 05/15/15 11:42 PM (9 years, 8 months ago) |
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Excellent work. Great procedure. Superb. Eatyu wins! Flawless Victory! Fatality!
How was it for you busting out all your old LC utensils, and employing/utilizing them again on some LC action?? Ha, bet you were like, "DAYUM, SUN!". 
Can't wait for you to start going around the world with LC's again, so you can begin offering your take on them (LC's). Outstanding Job!
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf] 1
#21689858 - 05/17/15 09:25 AM (9 years, 7 months ago) |
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Badass. Bringing back the memories. The syringe tips are eccellent and dont get clogged!
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hamloaf
Q-dood ®©™√



Registered: 12/23/09
Posts: 24,389
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: eatyualive]
#21689910 - 05/17/15 09:49 AM (9 years, 7 months ago) |
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Cool. Welcome back to the LC kingdom, eatyualive. It's been discovered that with the aid of magnetic stirring devices, syringes becoming clogged due to trying to aspire a large chuck of live culture is an issue of the past.
475cc's of WBS HLC aspired into recycled syringes.
Edited by hamloaf (05/17/15 10:18 AM)
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DeighFry
The Last Baron

Registered: 11/26/14
Posts: 200
Loc: Juniper
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf]
#21714922 - 05/23/15 05:11 PM (9 years, 7 months ago) |
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hamloaf, how is that grain water agar working out?
--------------------
Don't fake it baby.
Lay the real thing on me.
Spiraling up through the crack in the skye.
Leaving material world behind.
I see your face in constellations.
The martyr is ending his life for mine.
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: DeighFry] 1
#21721301 - 05/25/15 03:06 PM (9 years, 7 months ago) |
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here is 1cc of hamloaf liquid culture without a shake after 12 days. comparatively side by side with a LIquid inoculant of about 15cc of LI per jar after 13 days.
GWLC
 LI
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NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: eatyualive]
#21721525 - 05/25/15 03:58 PM (9 years, 7 months ago) |
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Fuckin sold. I saved about a gallon of grain water from my last soak session. I sterilized it in my old PC and now I'm letting it settle in the unopened PC for a while. In a few days I'm gonna decant some clarified grain water off and use that to make LC. I found some good deals on stir plates on eBay so I'm about to get serious.
--------------------
Let it grow! Let it grow! Greatly yield!
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in
DOG FOOD AGAR
MY ELECTRIC INOCULATION LOOP
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: NumeroEno] 1
#21722767 - 05/25/15 09:35 PM (9 years, 7 months ago) |
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greatest part is, i could use half of it, give it a few days and it will grow more. wasn't able to stir for a week. but all was well when i put the two lovely ladies on the stir plate! ill be inoculating a ton of that ape LC very shorty. i think apefest 2015 is about to begin again.
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insanemike

Registered: 02/23/14
Posts: 4,272
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: eatyualive]
#21722819 - 05/25/15 09:49 PM (9 years, 7 months ago) |
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I have fallen out of love with LC and have fallen in love with LI. I don't think I will ever go back.
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NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: eatyualive]
#21722890 - 05/25/15 10:13 PM (9 years, 7 months ago) |
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Quote:
eatyualive said: greatest part is, i could use half of it, give it a few days and it will grow more. wasn't able to stir for a week. but all was well when i put the two lovely ladies on the stir plate! ill be inoculating a ton of that ape LC very shorty. i think apefest 2015 is about to begin again.
I just finished PCing my grain water and it is hanging out in the fridge settling. After a few days in the fridge I'm gonna decant 2 quarts and save that. I'm buying a stir plate, stir bars, and some whatmans on Thursday. My plan was to do several tall half pints with my MS AA+ and GT, as well as the "red reishi" I got from grey and some king oyster.
Hopefully I can just start doing supplemented sawdust bags with the edibles if this LC works as well as I think it will.
--------------------
Let it grow! Let it grow! Greatly yield!
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in
DOG FOOD AGAR
MY ELECTRIC INOCULATION LOOP
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hamloaf
Q-dood ®©™√



Registered: 12/23/09
Posts: 24,389
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: NumeroEno]
#21723501 - 05/26/15 03:37 AM (9 years, 7 months ago) |
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For long term storage of your end-of-boil-cycle water place it into freezer, not fridge.
Also, don't inoculate your sterilized sawdust substrate(s) with LC unless you have added a sufficient amount of hydrated grains to your sawdust substrate(s) pre-sterilization. A good supplementation regimen for sawdust substrates is to add 5-10 parts wheat bran (or any grain flour), and 5% gypsum by volume.
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Dhearic


Registered: 01/24/14
Posts: 846
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf]
#21724165 - 05/26/15 09:30 AM (9 years, 7 months ago) |
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I collected about 1/2 a gallon of water after simmering my WBS + coffee after a 12 hour soak. Unfortunately I was out of gypsum at the time so there wasn't any in the soak water. It's now sitting in my freezer inside of a rinsed out milk container. Could you use the water collected after a Fooman prep? I.E. no soak time.
Looks like I'm gonna need to add a stir plate/bars on the list of shit to acquire. I plan on getting my LI setup going first though as I don't want to G2G nearly as much as I have to now lol Also I fucked myself with LC when I first started and again a few months ago when I was using pasty plates. I could never see well enough through my SAB then the pasty plate, perti infront of a hood ftw. 
How many jars do you all innoc with a single plate?
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Credit where credit is due.
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NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf]
#21724186 - 05/26/15 09:36 AM (9 years, 7 months ago) |
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Quote:
hamloaf said: For long term storage of your end-of-boil-cycle water place it into freezer, not fridge.
Also, don't inoculate your sterilized sawdust substrate(s) with LC unless you have added a sufficient amount of hydrated grains to your sawdust substrate(s) pre-sterilization. A good supplementation regimen for sawdust substrates is to add 5-10 parts wheat bran (or any grain flour), and 5% gypsum by volume.
I'm not storing it in the fridge long term, just until it settles. Then I'm gonna decant it and freeze it. For my sterilized bags I was planning on using a substrate recipe similar to muda's bottle subs. Whole grains and bran for sure, and maybe some fun stuff like bee pollen and flax seeds.
--------------------
Let it grow! Let it grow! Greatly yield!
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in
DOG FOOD AGAR
MY ELECTRIC INOCULATION LOOP
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NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: NumeroEno]
#21724191 - 05/26/15 09:39 AM (9 years, 7 months ago) |
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Also ham did you get that PM I sent you about the bag pasteurization? I think my method is pretty solid, and I just did some pan cyan sub in a bag last night, but I was wondering if you had anything to add.
--------------------
Let it grow! Let it grow! Greatly yield!
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in
DOG FOOD AGAR
MY ELECTRIC INOCULATION LOOP
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kushroom



Registered: 12/04/14
Posts: 588
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: eatyualive]
#21724481 - 05/26/15 11:23 AM (9 years, 7 months ago) |
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Quote:
eatyualive said: greatest part is, i could use half of it, give it a few days and it will grow more. wasn't able to stir for a week. but all was well when i put the two lovely ladies on the stir plate! ill be inoculating a ton of that ape LC very shorty. i think apefest 2015 is about to begin again.
oh shit!
-------------------- :/

All submitted posts are by Someone Who Isn't Me (SWIM) - and in any event are works of pure fiction or outright lies. Any information, statement, or assertion contained therein should be considered pure unadulterated fictitious lies.
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: kushroom] 1
#21725948 - 05/26/15 06:37 PM (9 years, 7 months ago) |
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here is my lc after 2 weeks. ive inoculated one round, plan on inoculating the stropharia next!
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Buck513

Registered: 04/17/14
Posts: 5,682
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: eatyualive]
#21725978 - 05/26/15 06:44 PM (9 years, 7 months ago) |
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Quote:
eatyualive said: here is my lc after 2 weeks. ive inoculated one round, plan on inoculating the stropharia next!

-------------------- Fail to plan and you plan to fail.
Enter the Ban Lottery
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: Dhearic]
#21725989 - 05/26/15 06:46 PM (9 years, 7 months ago) |
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Quote:
Dhearic said:

I collected about 1/2 a gallon of water after simmering my WBS + coffee after a 12 hour soak. Unfortunately I was out of gypsum at the time so there wasn't any in the soak water. It's now sitting in my freezer inside of a rinsed out milk container. Could you use the water collected after a Fooman prep? I.E. no soak time.
Looks like I'm gonna need to add a stir plate/bars on the list of shit to acquire. I plan on getting my LI setup going first though as I don't want to G2G nearly as much as I have to now lol Also I fucked myself with LC when I first started and again a few months ago when I was using pasty plates. I could never see well enough through my SAB then the pasty plate, perti infront of a hood ftw. 
How many jars do you all innoc with a single plate?
i used one dime size wedge in each of my 2 lc jars, now there is enough inoculum for at least 100 jars with each pint i used!
i think of g2g as manual labor now. lc is like me pressing the button so the machine does the hammering for me. instead of lifting the hammer. so you can be the hammer, or be hammered.
Edited by eatyualive (05/26/15 06:50 PM)
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Dhearic


Registered: 01/24/14
Posts: 846
Loc: Neverland
Last seen: 1 year, 8 months
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: eatyualive]
#21726278 - 05/26/15 08:02 PM (9 years, 7 months ago) |
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Quote:
so you can be the hammer, or be hammered.

Can I get hammered while being the hammer? Time to up my game with LI and pro style LC.
Any advice for constructing a diy stir plate? I remember reading something about a hard drive magnet but I may just end up buying a ready to use unit.
--------------------
Credit where credit is due.
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: Dhearic]
#21726358 - 05/26/15 08:15 PM (9 years, 7 months ago) |
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Dhearic


Registered: 01/24/14
Posts: 846
Loc: Neverland
Last seen: 1 year, 8 months
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: eatyualive]
#21726375 - 05/26/15 08:19 PM (9 years, 7 months ago) |
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Found me a project for next week. You rock eat!
--------------------
Credit where credit is due.
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hamloaf
Q-dood ®©™√



Registered: 12/23/09
Posts: 24,389
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Last seen: 1 day, 2 hours
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: NumeroEno]
#21731340 - 05/28/15 06:30 AM (9 years, 7 months ago) |
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Quote:
NumeroEno said: Also ham did you get that PM I sent you about the bag pasteurization? I think my method is pretty solid, and I just did some pan cyan sub in a bag last night, but I was wondering if you had anything to add.
Dude yer solid. Just be sure to monitor the internal core temp of EACH of your substrate bags very closely with a meat thermometer, because one thing that can happen when pasteurizing large bags like this is that the bags can, and will heat up at sightly different rates. Solution, as mentioned before, keep a close eye on your substrates' internal core temps. If one bag is increasing in temperature at a faster rate than the other simply turn the saucepan on the stove a full 180 degrees. Monitor your substrates' internal core temps every 20 minutes until you gain a better feel for how your pasteurization equipment will perform.
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Buck513

Registered: 04/17/14
Posts: 5,682
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf]
#21731351 - 05/28/15 06:38 AM (9 years, 7 months ago) |
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I liked you when you were a distinguished and sophisticated gentleman
-------------------- Fail to plan and you plan to fail.
Enter the Ban Lottery
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NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf]
#21731763 - 05/28/15 09:08 AM (9 years, 7 months ago) |
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Quote:
hamloaf said: Dude yer solid. Just be sure to monitor the internal core temp of EACH of your substrate bags very closely with a meat thermometer, because one thing that can happen when pasteurizing large bags like this is that the bags can, and will heat up at sightly different rates. Solution, as mentioned before, keep a close eye on your substrates' internal core temps. If one bag is increasing in temperature at a faster rate than the other simply turn the saucepan on the stove a full 180 degrees. Monitor your substrates' internal core temps every 20 minutes until you gain a better feel for how your pasteurization equipment will perform.
Good call. I've just been leaving the meat thermometer in one bag and setting the alarm. I'll start checking both bags. I can do enough substrate for 2 66 quart monotubs in one run using the bags, so I prefer it over jars for that reason. Anyway, back on topic.
Made this HLC yesterday with a wedge of GT from a plate that was forming primordia. It's probably about 5 transfers away from the original multispore germination.
I made a nice new lid with a whatman and put a shard of glass inside this one. It's also a much less rich solution. I noticed trich floating on top of the last one so it got tossed 
--------------------
Let it grow! Let it grow! Greatly yield!
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in
DOG FOOD AGAR
MY ELECTRIC INOCULATION LOOP
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hamloaf
Q-dood ®©™√



Registered: 12/23/09
Posts: 24,389
Loc: ation: Based.
Last seen: 1 day, 2 hours
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: NumeroEno]
#21731936 - 05/28/15 10:05 AM (9 years, 7 months ago) |
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Ah coolcool. Are you positive that trich was observed floating to the top of your LC, because often times, especially if temps get above 80F, and are sustained, bits/pieces of mushroom mycelium will float to the top, and begin colonizing the top of the media like that.
The solution is to try to split up the myce mat by shaking, stirring magnetically, or flame sterilizing some forceps, and "fishing" the mat of mycelium out of the culture. If the the mat of mycelium that forms on the top of LC's is left unchecked it will suffocate, and kill the live culture below.
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GreenRabbit
Plutonium Pollinator



Registered: 04/28/13
Posts: 2,667
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Last seen: 3 years, 3 months
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: Dhearic]
#21731972 - 05/28/15 10:16 AM (9 years, 7 months ago) |
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Quote:
Dhearic said:

I collected about 1/2 a gallon of water after simmering my WBS + coffee after a 12 hour soak. Unfortunately I was out of gypsum at the time so there wasn't any in the soak water. It's now sitting in my freezer inside of a rinsed out milk container. Could you use the water collected after a Fooman prep? I.E. no soak time.
Looks like I'm gonna need to add a stir plate/bars on the list of shit to acquire. I plan on getting my LI setup going first though as I don't want to G2G nearly as much as I have to now lol Also I fucked myself with LC when I first started and again a few months ago when I was using pasty plates. I could never see well enough through my SAB then the pasty plate, perti infront of a hood ftw. 
How many jars do you all innoc with a single plate?
I'm a little confused. Why are you guys saving your dunking water?
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Dhearic


Registered: 01/24/14
Posts: 846
Loc: Neverland
Last seen: 1 year, 8 months
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: GreenRabbit]
#21732017 - 05/28/15 10:30 AM (9 years, 7 months ago) |
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To use as a liquid culture media. Instead of using just a simple sugar like dextrose or a bit of honey you use the grain soak water.
From my understanding it has a more complex and full nutritional profile and gets the myc used to eating grain products before it's even on the grain.
I'm ready to try some new LCs in the coming weeks but I'm still cautious, lost a bunch of tubs to green a few months ago because of my noobness in LCs
--------------------
Credit where credit is due.
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NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf]
#21732085 - 05/28/15 10:56 AM (9 years, 7 months ago) |
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Quote:
hamloaf said: Ah coolcool. Are you positive that trich was observed floating to the top of your LC, because often times, especially if temps get above 80F, and are sustained, bits/pieces of mushroom mycelium will float to the top, and begin colonizing the top of the media like that.
The solution is to try to split up the myce mat by shaking, stirring magnetically, or flame sterilizing some forceps, and "fishing" the mat of mycelium out of the culture. If the the mat of mycelium that forms on the top of LC's is left unchecked it will suffocate, and kill the live culture below.
I'm pretty positive it was trich. I saw some green. I also made one with blue oyster that formed the mat you speak of. I think that on my first attempt, I made the media way too rich and had the lid off for too long during the inoculation.
I did this one way more carefully. Besides diluting the grain water, adding a shard of glass, and using a properly constructed lid with a whatman (my last lid just had a SFD), I was on point with the inoculation. I think this one is gonna do really well.
--------------------
Let it grow! Let it grow! Greatly yield!
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in
DOG FOOD AGAR
MY ELECTRIC INOCULATION LOOP
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: NumeroEno]
#21733442 - 05/28/15 05:20 PM (9 years, 7 months ago) |
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also stirring liquid cultures makes all the difference.
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Milk Man
Here to Fuck Your Wife

Registered: 05/15/15
Posts: 93
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: eatyualive]
#21733506 - 05/28/15 05:34 PM (9 years, 7 months ago) |
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I have a tub that was made with Hamloaf Liquid CULTURE!
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hamloaf
Q-dood ®©™√



Registered: 12/23/09
Posts: 24,389
Loc: ation: Based.
Last seen: 1 day, 2 hours
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: DeighFry]
#21735941 - 05/29/15 08:14 AM (9 years, 7 months ago) |
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Quote:
DeighFry said: hamloaf, how is that grain water agar working out?
Grain water agar is working out excellent, DeighFry. This will be the third time having a go with the grain water agar recipe. Nothing but luxuriant mycelial growth has ever been observed, so be prepared. You have the green light to proceed to the next step in the cultivation process with your grain water agar media. Good luck, and let us know how your trials, and tribulations with this new breed of media goes for you.
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hamloaf
Q-dood ®©™√



Registered: 12/23/09
Posts: 24,389
Loc: ation: Based.
Last seen: 1 day, 2 hours
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf]
#21757737 - 06/03/15 03:07 PM (9 years, 7 months ago) |
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1:4 diluted whole oat HLC featuring Panellus Stipticus.

1:4 diluted whole oat HLC featuring Agaricus Bitorquis.

Golden Teacher quart jar of 1:2 diluted Rye water, 20% horse manure, and 5% gypsum HLC both by volume before being stirred.

After being stirred.

The quart jar of PESH LC was neglected long enough for the old mat of mycelium to fully colonize the surface of the media killing/suffocating the culture below, and the Miatake LC contaminated with trich. 
The GT quart jar LC is for another round of testings with LC gun. The edible specie cultures are scheduled to be given away in the Marketplace.
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NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf]
#21788921 - 06/10/15 03:43 PM (9 years, 7 months ago) |
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Check it. Here are 2 jars. The one on the left was inoculated with 5cc of LI. I can't remember if it's GT or AA+. The LI might not be a fair comparison because it's old, but it was handy. Very soon I will be doing a head to head with PE6 LI and LC made from the same plate.
The jar on the right was inoculated with HLC. Full colonization in 7 days. That's way faster than agar drops.

The pe6 will be a better test, since the LI and LC will have come from the same plate. In fact, the LI was used to inoculate the LC. Once the LC grows a little more I will do identical grain jars.
--------------------
Let it grow! Let it grow! Greatly yield!
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in
DOG FOOD AGAR
MY ELECTRIC INOCULATION LOOP
Edited by NumeroEno (06/10/15 04:16 PM)
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oontribe

Registered: 01/14/15
Posts: 3,570
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: NumeroEno]
#21788946 - 06/10/15 03:47 PM (9 years, 7 months ago) |
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Gonna follow this one.
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: oontribe] 1
#21801543 - 06/13/15 10:11 AM (9 years, 7 months ago) |
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updated ape tubs. 2 days after spawn. i put some supercake in there. 50/50 coir/strawnet with a dash of gypsum. 5 quarts gwlc APE spawn.

i also wanted to update this with the pictures of the way i wrapped the foil around the syringe tip. this was done, so that when unwrapped, it could immediately and easily be placed on the sryinge. and take note, there was no flame sterilization of needle tip at all. it was sterilized prior to clean work in the pc at 15psi for 20 mins.
Quote:
i also used my nice utensil here. i wrapped tip in foil. making sure that the part that opens up is the part that screws on to the syringe body at the bottom of the picture below. i pressure cooked for 20 mins at 15psi. once cool, setup a sab, put all utensils, GWLC, Grain Jars and sterile syringe body still wrapped in the plastic inside the sab. i halfway unwrap the syringe body plastic not exposing the tip yet. i unfold the foil just enough so that when i pull the syringe body out, i immediately place the syringe plastic needle on the body. the rest of the foil is covering the syringe tip until the liquid is to be taken. i lifted the lid of the lc, sucked up the lc. closed the lid. lifted lid of receiving jar, squirted 1cc, then closed the lid. this was one steady motion in a sab. i didn't touch the syringe tip on anything and i did this for 15 jars in a row. I also don't want to melt these syringe tips.
syringe needle
   
Edited by eatyualive (06/13/15 11:54 AM)
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invitro

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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: eatyualive]
#21803140 - 06/13/15 05:09 PM (9 years, 7 months ago) |
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I noticed that syringe needle looks odd. It looks like it's 100% plastic. Is there a reason for that?
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eatyualive
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: invitro]
#21803430 - 06/13/15 06:38 PM (9 years, 7 months ago) |
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its autoclaveable and high gauge so that liquid cultures slide right through. the length of the needle is also about 2.5" so its easy to suck liquid cultures up if they are low in the jar. its specifically made for liquid culture.
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hamloaf
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: NumeroEno]
#21804709 - 06/14/15 01:55 AM (9 years, 7 months ago) |
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Bad-ass, Eno. Sorry, Didn't even see your post until eatyu posted his update too. Like the way you inoculated the LC with the LI. Textbook. HLC SHREDS grain substrates with exponential mycelial growth.
eatyu, you have an APE tub running? NICE! Good idea on autoclaving the needles like that for clean work. What is this "supercake" you spoke of "putting in there"?
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NumeroEno
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf]
#21805589 - 06/14/15 10:07 AM (9 years, 7 months ago) |
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Hell yeah man. The LC has grown enough that I think it's time. I'm prepping grain for this little test. I was amazed at how fast my last quart jar colonized with the LC, but I obviously screwed something up, because every jar I inoculated after the first one went bacterial. I guess I was a little lax with my sterile technique.
This time I won't be. I'm getting ready to aspire all of the LC into a 60cc syringe, and all of the inoculations will be done inside the SAB. Last time, with the GT LC, I aspired one 12cc syringe full to test it out, and did open air inoculations. I think I contaminated the culture when I took the first sample, because everything I inoculated with the second sample contaminated.
Luckily, that first clean quart jar served as a great backup, since I just finished g2g transferring it to 6 half gallons.
--------------------
Let it grow! Let it grow! Greatly yield!
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in
DOG FOOD AGAR
MY ELECTRIC INOCULATION LOOP
Edited by NumeroEno (06/14/15 10:09 AM)
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Grey
⇜ ✯ ⇝



Registered: 11/06/14
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: NumeroEno]
#21805723 - 06/14/15 10:55 AM (9 years, 7 months ago) |
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I made one with a wedge from my Galindoi stone clone. It's nice and thick, and about to be tested. I'm out if uploads at the moment. I'll upload a pic when that's sorted out.
--------------------
AMU Q&A
If you don't have a plan of your own, you'll become a part of somebody else's.
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NumeroEno
I come from the land of lizards



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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: Grey]
#21805747 - 06/14/15 11:04 AM (9 years, 7 months ago) |
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I just finished aspirating my PE6 LC. After I sucked up all the mycelium I smelled what was left in the jar and I'm positive it's totally clean. I'm about to inoculate 6 half gallon jars with the 60cc syringe.
Using LI to inoculate the LC is awesome!!! You don't get any thick clumps that are difficult to suck up. I just inoculated a new GT LC using this method. I think I'm gonna make a LI/LC with a tampanensis plate next and try to get a few bags of stones going.
--------------------
Let it grow! Let it grow! Greatly yield!
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in
DOG FOOD AGAR
MY ELECTRIC INOCULATION LOOP
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invitro

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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf]
#21879031 - 06/30/15 04:48 PM (9 years, 6 months ago) |
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Does GWLC ever really clarify enough to detect bacterial infection? I'm used to LME and I sometimes use cloudyness to indicate contamination. I'm using WBS, a few grains exploded which normally doesn't bother me but it might make the LC permanently cloudy I don't know.
Edited by invitro (06/30/15 04:50 PM)
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eatyualive
Eat's You Alive :)



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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: invitro]
#21879510 - 06/30/15 06:35 PM (9 years, 6 months ago) |
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This 2008 cultures is extremely old and showing. however, no stubbies this time. the substrate was allowed to colonize, placed into fruiting and a casing was added just before knotting.
there are about 5-7 early bloomers in there. no consolidation or anything. culture is looking fairly clean for a cranky old man.
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hamloaf
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: invitro]
#21880450 - 06/30/15 10:02 PM (9 years, 6 months ago) |
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Quote:
invitro said: Does GWLC ever really clarify enough to detect bacterial infection? I'm used to LME and I sometimes use cloudyness to indicate contamination. I'm using WBS, a few grains exploded which normally doesn't bother me but it might make the LC permanently cloudy I don't know.
This is largely going to depend on what grain was used to generate HLC, as well as, how much you dilute the media. Rye, and Milo are pretty murky even if you dilute the media down to the recommended 1:4 grain water to water ratio. Whole oats, and WBS are pretty clear when diluted down to the recommended 1:4. If your WBS is murky, and you used the 1:4 dilution ratio the assumption is going to be that your media is bacterial. When in doubt begin again though.
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invitro

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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf]
#21880480 - 06/30/15 10:07 PM (9 years, 6 months ago) |
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I did a GLC with WBS flour, super murky, never clears up (sat for a week uninoculated after pced for 45 min). I just did a batch with 5% exploded grains, again with the murkiness(have yet to pc it). I guess you can't have burst grains then if Ham is saying wbs is usually clear.
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invitro

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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: invitro]
#21880579 - 06/30/15 10:24 PM (9 years, 6 months ago) |
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Also, to clarify, I did not rinse the wbs. I added just enough water so that at the end of the heating of the grains the water level and the grain level were about equal, this leaves about 300ml of water for every 2 quarts of (hydrated)WBS. I also diluted 4:1 water to wbs soak water.
Edited by invitro (06/30/15 10:26 PM)
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hamloaf
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: invitro]
#21881767 - 07/01/15 06:22 AM (9 years, 6 months ago) |
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Quote:
invitro said: I did a GLC with WBS flour, super murky, never clears up (sat for a week uninoculated after pced for 45 min). I just did a batch with 5% exploded grains, again with the murkiness(have yet to pc it). I guess you can't have burst grains then if Ham is saying wbs is usually clear.
Quote:
invitro said: Also, to clarify, I did not rinse the wbs. I added just enough water so that at the end of the heating of the grains the water level and the grain level were about equal, this leaves about 300ml of water for every 2 quarts of (hydrated)WBS. I also diluted 4:1 water to wbs soak water.
Alright man. What we have here is a case of straying FAR away from the tek, then wondering why things aren't working. First of all, nice move making a LC out of grain flour. Answered a few questions with that experiment, but, man. How are going to half ass prep some WBS, not even sterilize the media, and complain about murkiness???
The suggestion is going to be, prep your WBS via any good soak, and boil/simmer tek. Rinse your WBS properly, soak, boil, then gather end-of-boil-cycle grain water to make the media out of. HLC media becomes much clearer, and a little bit darker after a run through the sterilizer.
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invitro

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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf]
#21882650 - 07/01/15 11:57 AM (9 years, 6 months ago) |
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I don't recall a rinse in the OP,lemme double check that. The flour was an experiment, just wanted to see what would happen for science. I wasn't complaining about murkiness, just making an observation and trying to confirm with your guys' experience. Your pic in the op is murky and there is no real explanation why so...
Let's switch from, this guy is "half-assing" the tek and complaining to, this guy is experimenting learning making observations and sharing notes and thoughts, trying to clarify....
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eatyualive
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf]
#21898984 - 07/04/15 10:49 PM (9 years, 6 months ago) |
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ham,
how many cc of liquid culture are you using per lets say a colonized grain quart jar?
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hamloaf
Q-dood ®©™√



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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: eatyualive]
#21899069 - 07/04/15 11:14 PM (9 years, 6 months ago) |
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About 1-3 cc's. Nothing too exact. Just depends on how much liquid culture there is to play with. If there is plenty of culture to go around, and/or if faster colonization is required, then higher amounts of liquid culture is used to inoculate quart jars with. It's preferred not to go over 3 cc's on quart jars as to not take a chance of ruining the hard worked for moisture content of the media.
When doing large bags the number of cc's that is used to inoculate quart jars with is multiplied by 10. 10-30 cc's of liquid culture is used. More specifically, on 4 quart bags 10-20 cc's of culture is used. When inoculating 8 quart bags with liquid culture - 20-30 cc's is used.
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf]
#21900130 - 07/05/15 09:00 AM (9 years, 6 months ago) |
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great thank you.
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NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: eatyualive]
#21900866 - 07/05/15 12:44 PM (9 years, 6 months ago) |
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I inoculated some half gallons of oats with about 10cc each on Wednesday. I think they'll be fully colonized soon.
I'm just about to the point where I can get a half gallon to fully colonize in about a week from LC, which renders g2g obsolete for me as far as cubes go. I'm just about to inoculate some half gallons with ape.
I've taken to doing LC inoculations inside the SAB. I use a sterile syringe and needle to aspirate the LC, and then take the lids off inside the SAB to inoculate. I have injection ports on all my lids but if I do the inoculations inside the SAB, the only thing that touches the LC is a sterile needle, and then the needle touches nothing for the grain inoculations. I think this eliminates at least one contam vector.
--------------------
Let it grow! Let it grow! Greatly yield!
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in
DOG FOOD AGAR
MY ELECTRIC INOCULATION LOOP
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: NumeroEno]
#21916671 - 07/08/15 07:59 PM (9 years, 6 months ago) |
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2nd flush coming in on the ape GWLC i posted earlier. didn't mist or anything and the flush just began. was going to mist but the pins popped up so quickly that it was not necessary. and the first flush shot out some monsters. not the stubby fuckers you see when cased.
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hamloaf
Q-dood ®©™√



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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: eatyualive]
#21917158 - 07/08/15 09:35 PM (9 years, 6 months ago) |
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That's great, eatyu. Those second flush pins look wonderful! Thanks for sharing, man.
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf]
#21917166 - 07/08/15 09:38 PM (9 years, 6 months ago) |
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yeah i just inoculated my stropharia gwlc. its been sitting for about 2 months with a 2 time per week stir on the plate. i have not had free jars until now to actually inoculate it.
ill update as it progresses.
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IMBiff
Learning Sponge

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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: eatyualive]
#21918556 - 07/09/15 09:11 AM (9 years, 6 months ago) |
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This is AWESOME!!
-------------------- I am here to be a sponge and to soak up as much wisdom as possible from the all knowing...I am grateful for you.
For noobs like me, click here:
http://www.shroomery.org/forums/showflat.php/Number/21938807
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Machiavelliavore
Vermiculite Hater



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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: IMBiff]
#21918575 - 07/09/15 09:18 AM (9 years, 6 months ago) |
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Trying to get one of these going. Assuming it works well, which my intuition says it will, it's hard to imagine buying all kinds of exotic sugars when you do this so easily.
Only exception is PF tek only people, and then can just chuck some brown rice in boiling water, get their GW, finish cooking their rice, and eat it for dinner.
--------------------
I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent.
Triggered yet?
Only a square would say "a cube is a cube."
No, this does not look right...
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hamloaf
Q-dood ®©™√



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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: Machiavelliavore]
#21918732 - 07/09/15 10:05 AM (9 years, 6 months ago) |
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Never done that before, but the intuition over boiling some brown rice to get end-of-boil-cycle grain water says that would suffice. Try it, see, post results here, or in your own thread. Not much boil water would be needed either, because the amount of boil water you gather can be multiplied by at least 4.
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spacechildo
proletarians rise up



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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf]
#21919239 - 07/09/15 10:51 AM (9 years, 6 months ago) |
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you can boil almost anything you like, carrots, beets, etc. rice included of course. doesnt have to be potatoes or grains  grain water just became popular because you already gonna need to cook some grains anyway.
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Pastywhyte
Say hello to my little friend



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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: spacechildo]
#21919600 - 07/09/15 11:48 AM (9 years, 6 months ago) |
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I did brown rice water once, it worked really good. Its a lot more starchy than most grains so watch for that. But it performs excellent.
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uninc4life2010
Unincorporated



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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: Pastywhyte]
#21977856 - 07/21/15 10:02 PM (9 years, 5 months ago) |
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I had a question concerning how you inoculate them. I don't see any rubber injection port on your jar lids, so I was wondering how you got the spores or what not in there. I have definitely had contamination problems with honey/karo liquid culture in the past, so is there any advantage to using this system over honey water?
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Mad Season
hookers and blackjack



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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: uninc4life2010]
#21977971 - 07/21/15 10:25 PM (9 years, 5 months ago) |
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All spores should get started on agar or else you'll have the same problems. Spores > lc isn't clean nor considered a safe practice.
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nobody83
stranger danger


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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: Mad Season]
#21978168 - 07/21/15 11:20 PM (9 years, 5 months ago) |
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Eat did you ever explain what the "super cake" was that you added to your sub? I didn't see if you did
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hamloaf
Q-dood ®©™√



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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: uninc4life2010]
#21978962 - 07/22/15 05:01 AM (9 years, 5 months ago) |
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Quote:
uninc4life2010 said: I had a question concerning how you inoculate them. I don't see any rubber injection port on your jar lids, so I was wondering how you got the spores or what not in there. I have definitely had contamination problems with honey/karo liquid culture in the past, so is there any advantage to using this system over honey water?
LC's are inoculated in this research lab by quickly cracking open the media closure and dropping an agar wedge into the freshly sterilized media inside the direct downstream of the flowhood.
As mentioned above, your contamination issue IS NOT a symptom of the type of media used. Your contamination issue rises from inoculation of liquid media with spores. UNLESS you have sterile spore print taking skills mushrooms are fruited in open air thus are contaminated a little bit. The degree of the contamination of the print is going to be determined on how clean of an environment said fruits were fruited inside of.
Place spores on agar with an inoculation loop inside of your still air box instead. Make the necessary amount of transfers needed until a clean culture culminates. Once clean culture on agar is generated, cut you a few pieces of agar no larger than a grain of rice from the VERY leading edge of the culture BEFORE the culture his the walls of the media vessel, and inoculate liquid media with the clean agar culture instead of spores.
The major advantage of utilizing grain water and employing it as a liquid media over Karo/honey/sugar in the raw, ect is that grain water liquid media generates a MUCH more vigorous culture than any sugar based liquid culture ever seen. Another advantage is the recycling of an otherwise waste product. The end-of-boil-cycle grain water.
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf]
#22016861 - 07/29/15 07:50 PM (9 years, 5 months ago) |
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some 4th flush ape from my gwlc!
i kind of forgot about this tub. set it in the corner under the table. found it, blowing up. i have not added water or misted since the dunk after the 2nd flush. i stopped paying attention after the 3rd flush. but today.
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nobody83
stranger danger


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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: eatyualive]
#22017598 - 07/29/15 10:16 PM (9 years, 5 months ago) |
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Clone?
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hamloaf
Q-dood ®©™√



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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: eatyualive]
#22020103 - 07/30/15 02:31 PM (9 years, 5 months ago) |
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Quote:
eatyualive said: some 4th flush ape from my gwlc!
i kind of forgot about this tub. set it in the corner under the table. found it, blowing up. i have not added water or misted since the dunk after the 2nd flush. i stopped paying attention after the 3rd flush. but today.

Excellent job, eatyualive! Those fruits look great.
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shellzenone
Zen



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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf]
#22117500 - 08/20/15 04:30 PM (9 years, 4 months ago) |
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stareatclouds
star eat clouds?



Registered: 09/29/14
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: shellzenone]
#22176515 - 09/01/15 11:34 PM (9 years, 4 months ago) |
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I'm going to give this a try in a bit here. I saved my water from boiling oats and am going to inoculate with a PE LI I have stored in the fridge. Going to freeze the HLC for now and then test out the viability of the PE LI on agar first to see if it's clean.
Is there any risk in storing in mason jars in the freezer?
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hamloaf
Q-dood ®©™√



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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: stareatclouds]
#22177069 - 09/02/15 05:20 AM (9 years, 4 months ago) |
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Nosir. With glass though I wouldn't put the jar with contents in the freezer while it's hot. Let everything cool to room temp before placing in the freezer.
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 10,361
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf]
#22177094 - 09/02/15 05:43 AM (9 years, 4 months ago) |
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For sure, I do that with everything I put in the fridge/freezer. Nothing worse than soggy food from the condensation dripping down, hehe. I wound up using Tupperware though. Going to noc these up with LI down the line and see if it outperforms sterilized distilled water with the same variety.
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nobody83
stranger danger


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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: stareatclouds]
#22179028 - 09/02/15 04:57 PM (9 years, 4 months ago) |
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Why you choose distilled?
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stareatclouds
star eat clouds?



Registered: 09/29/14
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: nobody83]
#22179048 - 09/02/15 05:01 PM (9 years, 4 months ago) |
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It's just what I've always used for it. I think eat uses it for his LI's and slurries. I'm no expert, but I think it promotes long term storage since there's no nutes.
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knomadic_niki
A mile high



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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: stareatclouds]
#24156966 - 03/12/17 06:38 PM (7 years, 10 months ago) |
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This is such a valuable resource. What a great fuckin idea! I hope it's okay that I dig it up. I just read it through and I have a couple questions.
First, I couldn't figure out what the difference is between "LI" and "LC". I know what an LC is, and I assume LI stands for Liquid Inoculant, but what makes an LI different from an LC?
Second, I haven't ever done LC's before, though Peter McCoy has shown me how several times. I was comfortable with agar and didn't see a need to learn a new technique. But now I'm stoked to get into it. I know there are good threads out there for LC noobs. Anyone care to embed them here for easy reference? The search function on this site stinks! I'd specifically like to know where to find autoclavable needles and syringes.
-------------------- My trade list
In search of sporeless oyster cultures
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,999
Loc:
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: knomadic_niki]
#24156991 - 03/12/17 06:49 PM (7 years, 10 months ago) |
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Quote:
The search function on this site stinks!
Usually it's the ability of the user that stinks. The search function works very well, you just have to get used to it.
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amidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: Josex]
#24157064 - 03/12/17 07:24 PM (7 years, 10 months ago) |
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.
-------------------- The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.
Edited by amidogen (06/21/18 10:39 AM)
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knomadic_niki
A mile high



Registered: 06/30/14
Posts: 1,275
Loc: 6200' Colorado
Last seen: 7 years, 5 months
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: amidogen]
#24157118 - 03/12/17 07:43 PM (7 years, 10 months ago) |
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thanks
-------------------- My trade list
In search of sporeless oyster cultures
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: Josex]
#24157338 - 03/12/17 09:15 PM (7 years, 10 months ago) |
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Quote:
Josex said:
Quote:
The search function on this site stinks!
Usually it's the ability of the user that stinks. The search function works very well, you just have to get used to it.
the search function is fuckin godly. Better than Google imo especially anything cultivation related.
Quote:
Mad Season said: Step 1: Use The Search Function (UTSF)

Lots of people complain the search function sucks, and gives outdated info. Try clicking the 2 pics above this!
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hamloaf
Q-dood ®©™√


Registered: 12/23/09
Posts: 24,389
Loc: ation: Based.
Last seen: 1 day, 2 hours
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: knomadic_niki]
#24157453 - 03/12/17 09:53 PM (7 years, 10 months ago) |
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Quote:
knomadic_niki said: This is such a valuable resource. What a great fuckin idea! I hope it's okay that I dig it up. I just read it through and I have a couple questions.
First, I couldn't figure out what the difference is between "LI" and "LC". I know what an LC is, and I assume LI stands for Liquid Inoculant, but what makes an LI different from an LC?
Second, I haven't ever done LC's before, though Peter McCoy has shown me how several times. I was comfortable with agar and didn't see a need to learn a new technique. But now I'm stoked to get into it. I know there are good threads out there for LC noobs. Anyone care to embed them here for easy reference? The search function on this site stinks! I'd specifically like to know where to find autoclavable needles and syringes.
Glass syringes are the only autoclave-able syringes that I know of.
I use plastic, presterilized, syringes. You can pick them up at tractor supply, or a veterinary wholesale supply to the public store.
The plastic syringes are reusable. Check user "Shea25"'s signature, and click on the link titled "How to clean a Syringe".
The difference between a LC, and LI is a nutrient source. LC has nutrients in the medium, and LI is sterilized water with culture on agar blended into the water forming an inoculant.
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knomadic_niki
A mile high



Registered: 06/30/14
Posts: 1,275
Loc: 6200' Colorado
Last seen: 7 years, 5 months
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: Mad Season]
#24157455 - 03/12/17 09:54 PM (7 years, 10 months ago) |
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Wow, I didn't even realize that was a button. I thought the search bar was all there was. Here I've been using the site for 7 years and somehow missed that. Thanks for highlighting it
-------------------- My trade list
In search of sporeless oyster cultures
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,999
Loc:
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf] 1
#24157916 - 03/13/17 02:40 AM (7 years, 10 months ago) |
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Quote:
hamloaf said:
Quote:
knomadic_niki said: This is such a valuable resource. What a great fuckin idea! I hope it's okay that I dig it up. I just read it through and I have a couple questions.
First, I couldn't figure out what the difference is between "LI" and "LC". I know what an LC is, and I assume LI stands for Liquid Inoculant, but what makes an LI different from an LC?
Second, I haven't ever done LC's before, though Peter McCoy has shown me how several times. I was comfortable with agar and didn't see a need to learn a new technique. But now I'm stoked to get into it. I know there are good threads out there for LC noobs. Anyone care to embed them here for easy reference? The search function on this site stinks! I'd specifically like to know where to find autoclavable needles and syringes.
Glass syringes are the only autoclave-able syringes that I know of.
I use plastic, presterilized, syringes. You can pick them up at tractor supply, or a veterinary wholesale supply to the public store.
The plastic syringes are reusable. Check user "Shea25"'s signature, and click on the link titled "How to clean a Syringe".
The difference between a LC, and LI is a nutrient source. LC has nutrients in the medium, and LI is sterilized water with culture on agar blended into the water forming an inoculant.
Plastic syringes are autoclavable as well, syringe, needle, needle cap... They are pp5.
I pc them all the time, being careful to place them standing up in the pc, that way they won't lose water.
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knomadic_niki
A mile high



Registered: 06/30/14
Posts: 1,275
Loc: 6200' Colorado
Last seen: 7 years, 5 months
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: Josex]
#24158305 - 03/13/17 09:41 AM (7 years, 10 months ago) |
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Thanks Josex, what do you mean so they don't lose water? So you can use the water it collects from condensation to do spore syringes or something?
Also, what brands do you use? I'm sure not all syringes/needles are the same.
-------------------- My trade list
In search of sporeless oyster cultures
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,999
Loc:
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: knomadic_niki]
#24158455 - 03/13/17 10:48 AM (7 years, 10 months ago) |
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I mean that if you fill them with water and pc them in other position other than standing up, water will boil out of the syringe during the pc cycle.
It doesn't matter the brand, size or if they are luer lock or luer slip... Plastic syringes for medical or veterinary use must follow an international standard. They won't melt on ya, try it yourself, if it doesn't work you'll be out of one miserable syringe, but if it works your life will be filled with new found hope and your faith in humanity will be restored
Edited by Josex (03/13/17 04:45 PM)
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hamloaf
Q-dood ®©™√


Registered: 12/23/09
Posts: 24,389
Loc: ation: Based.
Last seen: 1 day, 2 hours
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: Josex]
#24158483 - 03/13/17 11:02 AM (7 years, 10 months ago) |
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That's cool that you've found a way for plastic syringes to run through the PC.
I've always used the boiling water, 3 rinse method for sanatizing plastic syringes.
It's worked like a charm over, and over again, so it's never been questioned.
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knomadic_niki
A mile high



Registered: 06/30/14
Posts: 1,275
Loc: 6200' Colorado
Last seen: 7 years, 5 months
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: Josex]
#24158615 - 03/13/17 12:05 PM (7 years, 10 months ago) |
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that's great! throwing them in the pc with my LC and grain spawn will be super easy. so glad you chimed in, Josex. I could really use a new-found faith in humanity these days...
-------------------- My trade list
In search of sporeless oyster cultures
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blackout


Registered: 07/16/00
Posts: 5,266
Last seen: 1 year, 2 months
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: blackout]
#24158752 - 03/13/17 01:03 PM (7 years, 10 months ago) |
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Quote:
blackout said: egg whites can be used to clear wine & beer so I guess it would work with these LCs, you would only need a small bit of egg white for the typical volume of LC we use.
Got a PM about this asking if it should be heated, wine/beer makers to not heat it as far as I know.
But then I found this guy clearing chicken stock and he was heating it. Dunno if it would work with grain LCs though.
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knomadic_niki
A mile high



Registered: 06/30/14
Posts: 1,275
Loc: 6200' Colorado
Last seen: 7 years, 5 months
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: Josex]
#24255360 - 04/18/17 08:22 PM (7 years, 8 months ago) |
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Quote:
Josex said: I mean that if you fill them with water and pc them in other position other than standing up, water will boil out of the syringe during the pc cycle.
It doesn't matter the brand, size or if they are luer lock or luer slip... Plastic syringes for medical or veterinary use must follow an international standard. They won't melt on ya, try it yourself, if it doesn't work you'll be out of one miserable syringe, but if it works your life will be filled with new found hope and your faith in humanity will be restored
well, i tried pc'ing the syringes i had and they totally melted. just wanted to let the community know that not all syringes are autoclavable. what brand do you use?
-------------------- My trade list
In search of sporeless oyster cultures
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mynakedrat
The phantom hourglass


Registered: 02/16/17
Posts: 4,629
Loc: Inner Astral levels
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: knomadic_niki]
#24255872 - 04/18/17 11:51 PM (7 years, 8 months ago) |
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Ah man, they melted into your PC? Were they propped off the floor of the pan?
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Moabfighter
Tam Fighter


Registered: 12/13/15
Posts: 2,710
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: mynakedrat] 1
#24255938 - 04/19/17 12:26 AM (7 years, 8 months ago) |
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Damn. Got behind on Oat prep today and glad I did. Hopefully I have time tomorrow to make grains and try this. Thanks hamloaf!
-------------------- KSSS And PE WBS.
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,999
Loc:
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: knomadic_niki]
#24256080 - 04/19/17 02:48 AM (7 years, 8 months ago) |
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Quote:
knomadic_niki said:
Quote:
Josex said: I mean that if you fill them with water and pc them in other position other than standing up, water will boil out of the syringe during the pc cycle.
It doesn't matter the brand, size or if they are luer lock or luer slip... Plastic syringes for medical or veterinary use must follow an international standard. They won't melt on ya, try it yourself, if it doesn't work you'll be out of one miserable syringe, but if it works your life will be filled with new found hope and your faith in humanity will be restored
well, i tried pc'ing the syringes i had and they totally melted. just wanted to let the community know that not all syringes are autoclavable. what brand do you use?
Damn! What kinda syringe did you use? I use syringes for medical use, they're pp5, even the needle cap is pp5 and as far as I know all disposable plastic syringes for medical use must be pp5.
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hamloaf
Q-dood ®©™√


Registered: 12/23/09
Posts: 24,389
Loc: ation: Based.
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: Josex]
#24256286 - 04/19/17 06:42 AM (7 years, 8 months ago) |
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Quick, easy, bullet proof method of sterilizing syringes provided at the link below. https://www.shroomery.org/forums/showflat.php/Number/12098357#12098357
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knomadic_niki
A mile high



Registered: 06/30/14
Posts: 1,275
Loc: 6200' Colorado
Last seen: 7 years, 5 months
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: Josex]
#24256461 - 04/19/17 08:05 AM (7 years, 8 months ago) |
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Quote:
Josex said:
Quote:
knomadic_niki said:
Quote:
Josex said: I mean that if you fill them with water and pc them in other position other than standing up, water will boil out of the syringe during the pc cycle.
It doesn't matter the brand, size or if they are luer lock or luer slip... Plastic syringes for medical or veterinary use must follow an international standard. They won't melt on ya, try it yourself, if it doesn't work you'll be out of one miserable syringe, but if it works your life will be filled with new found hope and your faith in humanity will be restored
well, i tried pc'ing the syringes i had and they totally melted. just wanted to let the community know that not all syringes are autoclavable. what brand do you use?
Damn! What kinda syringe did you use? I use syringes for medical use, they're pp5, even the needle cap is pp5 and as far as I know all disposable plastic syringes for medical use must be pp5.
got them from the hospital (i'm an emt), so.... they're certainly medical grade. i wrapped it in tin foil and put it in the pc with my grain jars, resting on their lids. the syringes actually did okay, it was the needle tips that melted.
-------------------- My trade list
In search of sporeless oyster cultures
|
knomadic_niki
A mile high



Registered: 06/30/14
Posts: 1,275
Loc: 6200' Colorado
Last seen: 7 years, 5 months
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf]
#24256464 - 04/19/17 08:06 AM (7 years, 8 months ago) |
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Quote:
hamloaf said: Quick, easy, bullet proof method of sterilizing syringes provided at the link below. https://www.shroomery.org/forums/showflat.php/Number/12098357#12098357
i've read shea's tek. idk that i'd call that sterilization but it seems to work for enough people. would just be simpler if i could throw them in the pc with a batch of stuff instead of doing extra steps.
-------------------- My trade list
In search of sporeless oyster cultures
|
Josex
#cheat_code


Registered: 11/13/15
Posts: 8,999
Loc:
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: knomadic_niki]
#24256644 - 04/19/17 09:20 AM (7 years, 8 months ago) |
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Quote:
knomadic_niki said:
Quote:
Josex said:
Quote:
knomadic_niki said:
Quote:
Josex said: I mean that if you fill them with water and pc them in other position other than standing up, water will boil out of the syringe during the pc cycle.
It doesn't matter the brand, size or if they are luer lock or luer slip... Plastic syringes for medical or veterinary use must follow an international standard. They won't melt on ya, try it yourself, if it doesn't work you'll be out of one miserable syringe, but if it works your life will be filled with new found hope and your faith in humanity will be restored
well, i tried pc'ing the syringes i had and they totally melted. just wanted to let the community know that not all syringes are autoclavable. what brand do you use?
Damn! What kinda syringe did you use? I use syringes for medical use, they're pp5, even the needle cap is pp5 and as far as I know all disposable plastic syringes for medical use must be pp5.
got them from the hospital (i'm an emt), so.... they're certainly medical grade. i wrapped it in tin foil and put it in the pc with my grain jars, resting on their lids. the syringes actually did okay, it was the needle tips that melted.
Ok, gonna go ahead and FIFY 
Quote:
knomadic_niki said: well, i tried pc'ing the syringes i had and they the needle caps totally melted. just wanted to let the community know that not all syringes needle caps are autoclavable. what brand do you use?
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hamloaf
Q-dood ®©™√


Registered: 12/23/09
Posts: 24,389
Loc: ation: Based.
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: knomadic_niki]
#24256650 - 04/19/17 09:22 AM (7 years, 8 months ago) |
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You are correct. Technically it's "sanitation". Sterilization doesn't occur until 250F is reached. Sanatizing syringes that way was one of the first tricks I ever leaned in this hobby before I even had an account here, and I can tell you that the method is bulletproof. Been rocking some of the same syringes for a little over 5 years now. Plus clean syringes for LC, and spores can be achieved without a PC which seems to be one of Shea's themes. I miss that guy.
Anyway, I use Monoject brand 12 cc syringes sourced from a local ma and pa veterinary supply warehouse. They come 100 in a box for about 35 dollars. I have found this pack of syringes online for cheaper, but I don't mind, in fact I prefer to shell out the extra cash to support local businesses.
Personally I like not having to fire up the PC, and do a sterilization run just to generate clean, used syringes.
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knomadic_niki
A mile high



Registered: 06/30/14
Posts: 1,275
Loc: 6200' Colorado
Last seen: 7 years, 5 months
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: Josex]
#24256687 - 04/19/17 09:30 AM (7 years, 8 months ago) |
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no, not needle caps. the needles. i put the syringe with the needle attached (no cap) in the pc and the plastic part of the needle melted to the syringe.
-------------------- My trade list
In search of sporeless oyster cultures
Edited by knomadic_niki (04/19/17 09:35 AM)
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knomadic_niki
A mile high



Registered: 06/30/14
Posts: 1,275
Loc: 6200' Colorado
Last seen: 7 years, 5 months
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf]
#24256694 - 04/19/17 09:33 AM (7 years, 8 months ago) |
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Quote:
hamloaf said: You are correct. Technically it's "sanitation". Sterilization doesn't occur until 250F is reached. Sanatizing syringes that way was one of the first tricks I ever leaned in this hobby before I even had an account here, and I can tell you that the method is bulletproof. Been rocking some of the same syringes for a little over 5 years now. Plus clean syringes for LC, and spores can be achieved without a PC which seems to be one of Shea's themes. I miss that guy.
Anyway, I use Monoject brand 12 cc syringes sourced from a local ma and pa veterinary supply warehouse. They come 100 in a box for about 35 dollars. I have found this pack of syringes online for cheaper, but I don't mind, in fact I prefer to shell out the extra cash to support local businesses.
Personally I like not having to fire up the PC, and do a sterilization run just to generate clean, used syringes.
thanks for the info. i run the pc so frequently that it would be easier for me to just throw them in there with a load. but it's good to have a solid tek to fall back on. gotta snatch some new needles, though.
-------------------- My trade list
In search of sporeless oyster cultures
|
Josex
#cheat_code


Registered: 11/13/15
Posts: 8,999
Loc:
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: knomadic_niki]
#24256728 - 04/19/17 09:47 AM (7 years, 8 months ago) |
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Don't know then man. I've used 21G needles I can buy locally, 14G needles from an Ebay vendor and 18G needles from another Ebay vendor, all did fine in the PC 
Anyway, pcing syringes with water has been done lots before, it wasn't my idea at all, but it looks like that info was lost with time somehow, as I've seen lately several people, including 2 TC's, saying that you can't pc syringes, cos supposedly either the water boils out (which is true if you don't place them correctly in the pc) or the syringe melts, all this is a wrong info.
Pity Mudafuka or Cron aren't around, they know this very well IIRC.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,916
Loc: Milky way
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: Josex]
#24256760 - 04/19/17 09:56 AM (7 years, 8 months ago) |
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There's actually special air over steam cycles for autoclaving syringes in real labs. Because water does come out of them if you operate your pressure cooker correctly. We can get away with small losses and that's how muda and cron do it its possible to keep most of the water in the syringe but you cant defy physics
https://consteril.com/laboratory-autoclave-steam-sterilization-cycle-air-over-pressure/
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hamloaf
Q-dood ®©™√


Registered: 12/23/09
Posts: 24,389
Loc: ation: Based.
Last seen: 1 day, 2 hours
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: Josex]
#24256799 - 04/19/17 10:05 AM (7 years, 8 months ago) |
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Quote:
Josex said: Don't know then man. I've used 21G needles I can buy locally, 14G needles from an Ebay vendor and 18G needles from another Ebay vendor, all did fine in the PC 
Anyway, pcing syringes with water has been done lots before, it wasn't my idea at all, but it looks like that info was lost with time somehow, as I've seen lately several people, including 2 TC's, saying that you can't pc syringes, cos supposedly either the water boils out (which is true if you don't place them correctly in the pc) or the syringe melts, all this is a wrong info.
Pity Mudafuka or Cron aren't around, they know this very well IIRC.
Hi Josex.
Yo, you should correct people when, and if you find them to posting information that contradicts your experience. TC's aren't right 100% of the time. Nobody is. TC's are TC's because they have a knack for explaining things with pictures to back up their claims.
They also have a knack for finding, and utilizing universal methods of cultivation that will work for almost anybody no matter what the individual cultivator's unique growing situation is like. IMO/E, a good TC can also respectably admit he, or she is wrong.
P.S. I miss corn, and Muda too.
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,999
Loc:
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: bodhisatta]
#24256827 - 04/19/17 10:12 AM (7 years, 8 months ago) |
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Quote:
bodhisatta said: There's actually special air over steam cycles for autoclaving syringes in real labs. Because water does come out of them if you operate your pressure cooker correctly. We can get away with small losses and that's how muda and cron do it its possible to keep most of the water in the syringe but you cant defy physics
https://consteril.com/laboratory-autoclave-steam-sterilization-cycle-air-over-pressure/
 Bod I have to justno you my mang 
I've pc'd lots of syringes already and if there is a loss of water it is negligible at most, certainly not a difference you can notice just by looking at it.
I haven't tried pcing syringes without the needle cap, but pretty sure you can. I always pc them standing up in the pc (needle facing upwards) and propping them up against jars or even agar containers and I fill them with water till the highest mark, not more, and they don't seem to lose a drop of water at all 
I don't need to read anything contradicting this because I know from experience that it works.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,916
Loc: Milky way
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: Josex]
#24256836 - 04/19/17 10:14 AM (7 years, 8 months ago) |
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If you use a cap the water has no where to go... So no shit that works
If you PC a syringe with the needle on good luck.
caps suck. I leave needles on once theyre on. The caps that go over the needles don't really matter
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,999
Loc:
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: bodhisatta]
#24256863 - 04/19/17 10:25 AM (7 years, 8 months ago) |
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I don't have a syringe with me right now to show you, what I mean is a syringe with the needle on and then a separate pp5 cap that covers the needle, it's just a cover that fits most needles for medical use. Maybe you don't have that in the States? Stareatclouds posted a question some time ago in "general discussion" about spore syringes that led me to think that needle caps is not even a thing in the States.
What I do is, in the SAB, I unwrap the syringe, remove the cap with the needle still on and flame the needle to use (overkill, because the needle should be sterile).
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Gorlami



Registered: 08/28/13
Posts: 328
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: knomadic_niki]
#24256880 - 04/19/17 10:32 AM (7 years, 8 months ago) |
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Good info! Will start using Liquid Cultures once I get going with spawn bags. 
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hamloaf
Q-dood ®©™√


Registered: 12/23/09
Posts: 24,389
Loc: ation: Based.
Last seen: 1 day, 2 hours
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: Josex]
#24256898 - 04/19/17 10:37 AM (7 years, 8 months ago) |
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We have needle caps here in the states, although we could be referring to 2 entirely different things.
Are the needle caps that you are referring to the same thing as the orange caps on the syringes featured in the pic below?
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,999
Loc:
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf]
#24256909 - 04/19/17 10:41 AM (7 years, 8 months ago) |
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No Ham, it's quite different. The cap in the pic really looks like it would melt in the pc. I'll try to find pics on google to show you.
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,999
Loc:
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: Josex]
#24256925 - 04/19/17 10:48 AM (7 years, 8 months ago) |
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Josex
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: Josex]
#24256939 - 04/19/17 10:53 AM (7 years, 8 months ago) |
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They are always transparent btw and you can pc the shit out of them
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hamloaf
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: Josex]
#24256950 - 04/19/17 10:55 AM (7 years, 8 months ago) |
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Interesting. Wonder if those little caps that vendors send syringes out with are autoclavable. They look a lot like what you have posted, but what you have posted would be better, so one wouldn't have to mess with getting the needle sterilized separately, then having to get the sterilized parts together again. Thanks for sharing!
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Josex
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf]
#24256995 - 04/19/17 11:12 AM (7 years, 8 months ago) |
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Oh shit, and to think that I wrote the LC tek in my journal thinking that everybody has access to those types of caps For the few of you that read my journal and was like wtf!? You really need those caps to do the method.
On the other hand, glad we have something kewl here in Europe you folks from the Statea don't have, for a fucking change
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mynakedrat
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: Josex]
#24257086 - 04/19/17 11:42 AM (7 years, 8 months ago) |
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All mine have caps. Just Like that, and I'm in the USA.... 'Murka! Fuck yea!
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bodhisatta 
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: Josex]
#24257092 - 04/19/17 11:44 AM (7 years, 8 months ago) |
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We have both caps. The cap that goes over the needle hasn't held water in a syringe for me in the past.
The "syringe plugs" will
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Josex
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: bodhisatta]
#24257847 - 04/19/17 05:27 PM (7 years, 8 months ago) |
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Yeah I guessed it wouldn't hold water at all, I just use them to prevent piercing the foil and to keep the needle sterile when you unwrap the syringe. The only thing to prevent water from boiling out is putting the syringes upright in the pc, needle caps have nothing to do with this.
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Skipio
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf]
#25351492 - 07/28/18 12:02 AM (6 years, 5 months ago) |
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Quote:
hamloaf said:...cool to room temp...
Room temp (~72°F) to inoculate too?
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NumeroEno
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: Skipio]
#25351503 - 07/28/18 12:14 AM (6 years, 5 months ago) |
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Man I almost tried this again today but I didn’t have a LC lid made up.
Quote:
Skipio said:
Quote:
hamloaf said:...cool to room temp...
Room temp (~72°F) to inoculate too?
Yes. You always want your media to be room temperature when you inoculate it, whether it be agar, LC, or grain.
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Let it grow! Let it grow! Greatly yield!
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in
DOG FOOD AGAR
MY ELECTRIC INOCULATION LOOP
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sytheii
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf]
#27809602 - 06/07/22 11:41 AM (2 years, 7 months ago) |
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Ham, or others,
I know this is an older thread, and it's possible there are other more established LC threads out there, but I liked the simplicity of this preparation.
I am wondering if it might be possible to update the original post with a couple of key points that I wasn't able to suss out with 100% confidence after reading the whole thread.
After inoculation of your GWLC, what is the gameplan after that?
Do we stir plate every two days? Do we wait a set period, like 2 weeks, and then stir up?
How much is best to stir, what frequency? If plate not handy, and we are using glass shard in jar...what is sufficient?
How long can GWLC be on the shelf, shaken up and used? Is there a point at which it's dangerous to inoculate, even if you're already sure that the contents are contam free?
Is it dumb to store LC in the fridge?
Any additional info you could think to add to OP would be dope to cover the questions i can think to ask, and I'm sure there are points that I'm forgetting.
For getting the LC out of the jar the best method i could envision was a sterile syringe, draw material in, then remove, in SAB or in front of flow hood.
Thanks
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