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Offlineblackout
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: blackout]
    #24158752 - 03/13/17 01:03 PM (7 years, 10 months ago)

Quote:

blackout said:
egg whites can be used to clear wine & beer so I guess it would work with these LCs, you would only need a small bit of egg white for the typical volume of LC we use.



Got a PM about this asking if it should be heated, wine/beer makers to not heat it as far as I know.

But then I found this guy clearing chicken stock and he was heating it. Dunno if it would work with grain LCs though.


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Offlineknomadic_niki
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: Josex]
    #24255360 - 04/18/17 08:22 PM (7 years, 8 months ago)

Quote:

Josex said:
I mean that if you fill them with water and pc them in other position other than standing up, water will boil out of the syringe during the pc cycle.

It doesn't matter the brand, size or if they are luer lock or luer slip... Plastic syringes for medical or veterinary use must follow an international standard. They won't melt on ya, try it yourself, if it doesn't work you'll be out of one miserable syringe, but if it works your life will be filled with new found hope and your faith in humanity will be restored :crazy2:




well, i tried pc'ing the syringes i had and they totally melted. just wanted to let the community know that not all syringes are autoclavable. what brand do you use?


--------------------
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In search of sporeless oyster cultures

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Invisiblemynakedrat
The phantom hourglass
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Registered: 02/16/17
Posts: 4,629
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: knomadic_niki]
    #24255872 - 04/18/17 11:51 PM (7 years, 8 months ago)

Ah man, they melted into your PC?
Were they propped off the floor of the pan?

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InvisibleMoabfighter
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: mynakedrat] * 1
    #24255938 - 04/19/17 12:26 AM (7 years, 8 months ago)

Damn. Got behind on Oat prep today and glad I did. Hopefully I have time tomorrow to make grains and try this. Thanks hamloaf!


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InvisibleJosex
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: knomadic_niki]
    #24256080 - 04/19/17 02:48 AM (7 years, 8 months ago)

Quote:

knomadic_niki said:
Quote:

Josex said:
I mean that if you fill them with water and pc them in other position other than standing up, water will boil out of the syringe during the pc cycle.

It doesn't matter the brand, size or if they are luer lock or luer slip... Plastic syringes for medical or veterinary use must follow an international standard. They won't melt on ya, try it yourself, if it doesn't work you'll be out of one miserable syringe, but if it works your life will be filled with new found hope and your faith in humanity will be restored :crazy2:




well, i tried pc'ing the syringes i had and they totally melted. just wanted to let the community know that not all syringes are autoclavable. what brand do you use?




Damn! What kinda syringe did you use? I use syringes for medical use, they're pp5, even the needle cap is pp5 :shrug: and as far as I know all disposable plastic syringes for medical use must be pp5.

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OfflinehamloafM
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: Josex]
    #24256286 - 04/19/17 06:42 AM (7 years, 8 months ago)

Quick, easy, bullet proof method of sterilizing syringes provided at the link below. 
https://www.shroomery.org/forums/showflat.php/Number/12098357#12098357


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How I Get Stuff done. - My Reference Guide. - My Grows.

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Offlineknomadic_niki
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: Josex]
    #24256461 - 04/19/17 08:05 AM (7 years, 8 months ago)

Quote:

Josex said:
Quote:

knomadic_niki said:
Quote:

Josex said:
I mean that if you fill them with water and pc them in other position other than standing up, water will boil out of the syringe during the pc cycle.

It doesn't matter the brand, size or if they are luer lock or luer slip... Plastic syringes for medical or veterinary use must follow an international standard. They won't melt on ya, try it yourself, if it doesn't work you'll be out of one miserable syringe, but if it works your life will be filled with new found hope and your faith in humanity will be restored :crazy2:




well, i tried pc'ing the syringes i had and they totally melted. just wanted to let the community know that not all syringes are autoclavable. what brand do you use?




Damn! What kinda syringe did you use? I use syringes for medical use, they're pp5, even the needle cap is pp5 :shrug: and as far as I know all disposable plastic syringes for medical use must be pp5.




got them from the hospital (i'm an emt), so.... they're certainly medical grade. i wrapped it in tin foil and put it in the pc with my grain jars, resting on their lids. the syringes actually did okay, it was the needle tips that melted.


--------------------
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In search of sporeless oyster cultures

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Offlineknomadic_niki
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf]
    #24256464 - 04/19/17 08:06 AM (7 years, 8 months ago)

Quote:

hamloaf said:
Quick, easy, bullet proof method of sterilizing syringes provided at the link below. 
https://www.shroomery.org/forums/showflat.php/Number/12098357#12098357




i've read shea's tek. idk that i'd call that sterilization but it seems to work for enough people. would just be simpler if i could throw them in the pc with a batch of stuff instead of doing extra steps.


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In search of sporeless oyster cultures

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InvisibleJosex
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: knomadic_niki]
    #24256644 - 04/19/17 09:20 AM (7 years, 8 months ago)

Quote:

knomadic_niki said:
Quote:

Josex said:
Quote:

knomadic_niki said:
Quote:

Josex said:
I mean that if you fill them with water and pc them in other position other than standing up, water will boil out of the syringe during the pc cycle.

It doesn't matter the brand, size or if they are luer lock or luer slip... Plastic syringes for medical or veterinary use must follow an international standard. They won't melt on ya, try it yourself, if it doesn't work you'll be out of one miserable syringe, but if it works your life will be filled with new found hope and your faith in humanity will be restored :crazy2:




well, i tried pc'ing the syringes i had and they totally melted. just wanted to let the community know that not all syringes are autoclavable. what brand do you use?




Damn! What kinda syringe did you use? I use syringes for medical use, they're pp5, even the needle cap is pp5 :shrug: and as far as I know all disposable plastic syringes for medical use must be pp5.




got them from the hospital (i'm an emt), so.... they're certainly medical grade. i wrapped it in tin foil and put it in the pc with my grain jars, resting on their lids. the syringes actually did okay, it was the needle tips that melted.




Ok, gonna go ahead and FIFY :super:
Quote:

knomadic_niki said:
well, i tried pc'ing the syringes i had and they the needle caps totally melted. just wanted to let the community know that not all syringes needle caps are autoclavable. what brand do you use?



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OfflinehamloafM
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: knomadic_niki]
    #24256650 - 04/19/17 09:22 AM (7 years, 8 months ago)

You are correct.  Technically it's "sanitation".  Sterilization doesn't occur until 250F is reached.  Sanatizing syringes that way was one of the first tricks I ever leaned in this hobby before I even had an account here, and I can tell you that the method is bulletproof.  Been rocking some of the same syringes for a little over 5 years now.  Plus clean syringes for LC, and spores can be achieved without a PC which seems to be one of Shea's themes.  I miss that guy. 

Anyway, I use Monoject brand 12 cc syringes sourced from a local ma and pa veterinary supply warehouse.  They come 100 in a box for about 35 dollars.  I have found this pack of syringes online for cheaper, but I don't mind, in fact I prefer to shell out the extra cash to support local businesses.

Personally I like not having to fire up the PC, and do a sterilization run just to generate clean, used syringes.


--------------------
     
How I Get Stuff done. - My Reference Guide. - My Grows.

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Offlineknomadic_niki
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: Josex]
    #24256687 - 04/19/17 09:30 AM (7 years, 8 months ago)

no, not needle caps. the needles. i put the syringe with the needle attached (no cap) in the pc and the plastic part of the needle melted to the syringe.


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In search of sporeless oyster cultures

Edited by knomadic_niki (04/19/17 09:35 AM)

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Offlineknomadic_niki
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf]
    #24256694 - 04/19/17 09:33 AM (7 years, 8 months ago)

Quote:

hamloaf said:
You are correct.  Technically it's "sanitation".  Sterilization doesn't occur until 250F is reached.  Sanatizing syringes that way was one of the first tricks I ever leaned in this hobby before I even had an account here, and I can tell you that the method is bulletproof.  Been rocking some of the same syringes for a little over 5 years now.  Plus clean syringes for LC, and spores can be achieved without a PC which seems to be one of Shea's themes.  I miss that guy. 

Anyway, I use Monoject brand 12 cc syringes sourced from a local ma and pa veterinary supply warehouse.  They come 100 in a box for about 35 dollars.  I have found this pack of syringes online for cheaper, but I don't mind, in fact I prefer to shell out the extra cash to support local businesses.

Personally I like not having to fire up the PC, and do a sterilization run just to generate clean, used syringes.




thanks for the info. i run the pc so frequently that it would be easier for me to just throw them in there with a load. but it's good to have a solid tek to fall back on. gotta snatch some new needles, though.


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In search of sporeless oyster cultures

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InvisibleJosex
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: knomadic_niki]
    #24256728 - 04/19/17 09:47 AM (7 years, 8 months ago)

Don't know then man. I've used 21G needles I can buy locally, 14G needles from an Ebay vendor and 18G needles from another Ebay vendor, all did fine in the PC :shrug:

Anyway, pcing syringes with water has been done lots before, it wasn't my idea at all, but it looks like that info was lost with time somehow, as I've seen lately several people, including 2 TC's, saying that you can't pc syringes, cos supposedly either the water boils out (which is true if you don't place them correctly in the pc) or the syringe melts, all this is a wrong info.

Pity Mudafuka or Cron aren't around, they know this very well IIRC.

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InvisiblebodhisattaMDiscordReddit
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: Josex]
    #24256760 - 04/19/17 09:56 AM (7 years, 8 months ago)

There's actually special air over steam cycles for autoclaving syringes in real labs. Because water does come out of them if you operate your pressure cooker correctly. We can get away with small losses and that's how muda and cron do it its possible to keep most of the water in the syringe but you cant defy physics

https://consteril.com/laboratory-autoclave-steam-sterilization-cycle-air-over-pressure/

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OfflinehamloafM
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: Josex]
    #24256799 - 04/19/17 10:05 AM (7 years, 8 months ago)

Quote:

Josex said:
Don't know then man. I've used 21G needles I can buy locally, 14G needles from an Ebay vendor and 18G needles from another Ebay vendor, all did fine in the PC :shrug:

Anyway, pcing syringes with water has been done lots before, it wasn't my idea at all, but it looks like that info was lost with time somehow, as I've seen lately several people, including 2 TC's, saying that you can't pc syringes, cos supposedly either the water boils out (which is true if you don't place them correctly in the pc) or the syringe melts, all this is a wrong info.

Pity Mudafuka or Cron aren't around, they know this very well IIRC.



Hi Josex. 

Yo, you should correct people when, and if you find them to posting information that contradicts your experience.  TC's aren't right 100% of the time.  Nobody is.  TC's are TC's because they have a knack for explaining things with pictures to back up their claims. 

They also have a knack for finding, and utilizing universal methods of cultivation that will work for almost anybody no matter what the individual cultivator's unique growing situation is like.  IMO/E, a good TC can also respectably admit he, or she is wrong. 

P.S.  I miss corn, and Muda too.


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How I Get Stuff done. - My Reference Guide. - My Grows.

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InvisibleJosex
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: bodhisatta]
    #24256827 - 04/19/17 10:12 AM (7 years, 8 months ago)

Quote:

bodhisatta said:
There's actually special air over steam cycles for autoclaving syringes in real labs. Because water does come out of them if you operate your pressure cooker correctly. We can get away with small losses and that's how muda and cron do it its possible to keep most of the water in the syringe but you cant defy physics

https://consteril.com/laboratory-autoclave-steam-sterilization-cycle-air-over-pressure/




:justno:
Bod I have to justno you my mang :lmafo:


I've pc'd lots of syringes already and if there is a loss of water it is negligible at most, certainly not a difference you can notice just by looking at it.

I haven't tried pcing syringes without the needle cap, but pretty sure you can. I always pc them standing up in the pc (needle facing upwards) and propping them up against jars or even agar containers and I fill them with water till the highest mark, not more, and they don't seem to lose a drop of water at all :shrug:

I don't need to read anything contradicting this because I know from experience that it works.

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InvisiblebodhisattaMDiscordReddit
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: Josex]
    #24256836 - 04/19/17 10:14 AM (7 years, 8 months ago)

If you use a cap the water has no where to go... So no shit that works

If you PC a syringe with the needle on good luck.

caps suck. I leave needles on once theyre on. The caps that go over the needles don't really matter

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InvisibleJosex
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: bodhisatta]
    #24256863 - 04/19/17 10:25 AM (7 years, 8 months ago)

I don't have a syringe with me right now to show you, what I mean is a syringe with the needle on and then a separate pp5 cap that covers the needle, it's just a cover that fits most needles for medical use. Maybe you don't have that in the States? Stareatclouds posted a question some time ago in "general discussion" about spore syringes that led me to think that needle caps is not even a thing in the States.

What I do is, in the SAB, I unwrap the syringe, remove the cap with the needle still on and flame the needle to use (overkill, because the needle should be sterile).

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InvisibleGorlami
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: knomadic_niki]
    #24256880 - 04/19/17 10:32 AM (7 years, 8 months ago)

Good info!  Will start using Liquid Cultures once I get going with spawn bags. :laugh:

:takingnotes:

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OfflinehamloafM
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: Josex]
    #24256898 - 04/19/17 10:37 AM (7 years, 8 months ago)

We have needle caps here in the states, although we could be referring to 2 entirely different things.

Are the needle caps that you are referring to the same thing as the orange caps on the syringes featured in the pic below?


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How I Get Stuff done. - My Reference Guide. - My Grows.

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