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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: DeighFry] 1
#21721301 - 05/25/15 03:06 PM (9 years, 7 months ago) |
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here is 1cc of hamloaf liquid culture without a shake after 12 days. comparatively side by side with a LIquid inoculant of about 15cc of LI per jar after 13 days.
GWLC
 LI
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NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: eatyualive]
#21721525 - 05/25/15 03:58 PM (9 years, 7 months ago) |
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Fuckin sold. I saved about a gallon of grain water from my last soak session. I sterilized it in my old PC and now I'm letting it settle in the unopened PC for a while. In a few days I'm gonna decant some clarified grain water off and use that to make LC. I found some good deals on stir plates on eBay so I'm about to get serious.
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Let it grow! Let it grow! Greatly yield!
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in
DOG FOOD AGAR
MY ELECTRIC INOCULATION LOOP
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: NumeroEno] 1
#21722767 - 05/25/15 09:35 PM (9 years, 7 months ago) |
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greatest part is, i could use half of it, give it a few days and it will grow more. wasn't able to stir for a week. but all was well when i put the two lovely ladies on the stir plate! ill be inoculating a ton of that ape LC very shorty. i think apefest 2015 is about to begin again.
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insanemike

Registered: 02/23/14
Posts: 4,272
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: eatyualive]
#21722819 - 05/25/15 09:49 PM (9 years, 7 months ago) |
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I have fallen out of love with LC and have fallen in love with LI. I don't think I will ever go back.
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NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: eatyualive]
#21722890 - 05/25/15 10:13 PM (9 years, 7 months ago) |
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Quote:
eatyualive said: greatest part is, i could use half of it, give it a few days and it will grow more. wasn't able to stir for a week. but all was well when i put the two lovely ladies on the stir plate! ill be inoculating a ton of that ape LC very shorty. i think apefest 2015 is about to begin again.
I just finished PCing my grain water and it is hanging out in the fridge settling. After a few days in the fridge I'm gonna decant 2 quarts and save that. I'm buying a stir plate, stir bars, and some whatmans on Thursday. My plan was to do several tall half pints with my MS AA+ and GT, as well as the "red reishi" I got from grey and some king oyster.
Hopefully I can just start doing supplemented sawdust bags with the edibles if this LC works as well as I think it will.
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Let it grow! Let it grow! Greatly yield!
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in
DOG FOOD AGAR
MY ELECTRIC INOCULATION LOOP
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hamloaf
Q-dood ®©™√



Registered: 12/23/09
Posts: 24,389
Loc: ation: Based.
Last seen: 1 day, 2 hours
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: NumeroEno]
#21723501 - 05/26/15 03:37 AM (9 years, 7 months ago) |
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For long term storage of your end-of-boil-cycle water place it into freezer, not fridge.
Also, don't inoculate your sterilized sawdust substrate(s) with LC unless you have added a sufficient amount of hydrated grains to your sawdust substrate(s) pre-sterilization. A good supplementation regimen for sawdust substrates is to add 5-10 parts wheat bran (or any grain flour), and 5% gypsum by volume.
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Dhearic


Registered: 01/24/14
Posts: 846
Loc: Neverland
Last seen: 1 year, 8 months
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf]
#21724165 - 05/26/15 09:30 AM (9 years, 7 months ago) |
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I collected about 1/2 a gallon of water after simmering my WBS + coffee after a 12 hour soak. Unfortunately I was out of gypsum at the time so there wasn't any in the soak water. It's now sitting in my freezer inside of a rinsed out milk container. Could you use the water collected after a Fooman prep? I.E. no soak time.
Looks like I'm gonna need to add a stir plate/bars on the list of shit to acquire. I plan on getting my LI setup going first though as I don't want to G2G nearly as much as I have to now lol Also I fucked myself with LC when I first started and again a few months ago when I was using pasty plates. I could never see well enough through my SAB then the pasty plate, perti infront of a hood ftw. 
How many jars do you all innoc with a single plate?
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Credit where credit is due.
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NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf]
#21724186 - 05/26/15 09:36 AM (9 years, 7 months ago) |
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Quote:
hamloaf said: For long term storage of your end-of-boil-cycle water place it into freezer, not fridge.
Also, don't inoculate your sterilized sawdust substrate(s) with LC unless you have added a sufficient amount of hydrated grains to your sawdust substrate(s) pre-sterilization. A good supplementation regimen for sawdust substrates is to add 5-10 parts wheat bran (or any grain flour), and 5% gypsum by volume.
I'm not storing it in the fridge long term, just until it settles. Then I'm gonna decant it and freeze it. For my sterilized bags I was planning on using a substrate recipe similar to muda's bottle subs. Whole grains and bran for sure, and maybe some fun stuff like bee pollen and flax seeds.
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Let it grow! Let it grow! Greatly yield!
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in
DOG FOOD AGAR
MY ELECTRIC INOCULATION LOOP
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NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: NumeroEno]
#21724191 - 05/26/15 09:39 AM (9 years, 7 months ago) |
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Also ham did you get that PM I sent you about the bag pasteurization? I think my method is pretty solid, and I just did some pan cyan sub in a bag last night, but I was wondering if you had anything to add.
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Let it grow! Let it grow! Greatly yield!
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in
DOG FOOD AGAR
MY ELECTRIC INOCULATION LOOP
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kushroom



Registered: 12/04/14
Posts: 588
Loc: I'm lost
Last seen: 8 months, 20 days
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: eatyualive]
#21724481 - 05/26/15 11:23 AM (9 years, 7 months ago) |
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Quote:
eatyualive said: greatest part is, i could use half of it, give it a few days and it will grow more. wasn't able to stir for a week. but all was well when i put the two lovely ladies on the stir plate! ill be inoculating a ton of that ape LC very shorty. i think apefest 2015 is about to begin again.
oh shit!
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All submitted posts are by Someone Who Isn't Me (SWIM) - and in any event are works of pure fiction or outright lies. Any information, statement, or assertion contained therein should be considered pure unadulterated fictitious lies.
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: kushroom] 1
#21725948 - 05/26/15 06:37 PM (9 years, 7 months ago) |
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here is my lc after 2 weeks. ive inoculated one round, plan on inoculating the stropharia next!
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Buck513

Registered: 04/17/14
Posts: 5,682
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: eatyualive]
#21725978 - 05/26/15 06:44 PM (9 years, 7 months ago) |
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Quote:
eatyualive said: here is my lc after 2 weeks. ive inoculated one round, plan on inoculating the stropharia next!

-------------------- Fail to plan and you plan to fail.
Enter the Ban Lottery
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: Dhearic]
#21725989 - 05/26/15 06:46 PM (9 years, 7 months ago) |
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Quote:
Dhearic said:

I collected about 1/2 a gallon of water after simmering my WBS + coffee after a 12 hour soak. Unfortunately I was out of gypsum at the time so there wasn't any in the soak water. It's now sitting in my freezer inside of a rinsed out milk container. Could you use the water collected after a Fooman prep? I.E. no soak time.
Looks like I'm gonna need to add a stir plate/bars on the list of shit to acquire. I plan on getting my LI setup going first though as I don't want to G2G nearly as much as I have to now lol Also I fucked myself with LC when I first started and again a few months ago when I was using pasty plates. I could never see well enough through my SAB then the pasty plate, perti infront of a hood ftw. 
How many jars do you all innoc with a single plate?
i used one dime size wedge in each of my 2 lc jars, now there is enough inoculum for at least 100 jars with each pint i used!
i think of g2g as manual labor now. lc is like me pressing the button so the machine does the hammering for me. instead of lifting the hammer. so you can be the hammer, or be hammered.
Edited by eatyualive (05/26/15 06:50 PM)
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Dhearic


Registered: 01/24/14
Posts: 846
Loc: Neverland
Last seen: 1 year, 8 months
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: eatyualive]
#21726278 - 05/26/15 08:02 PM (9 years, 7 months ago) |
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Quote:
so you can be the hammer, or be hammered.

Can I get hammered while being the hammer? Time to up my game with LI and pro style LC.
Any advice for constructing a diy stir plate? I remember reading something about a hard drive magnet but I may just end up buying a ready to use unit.
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Credit where credit is due.
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: Dhearic]
#21726358 - 05/26/15 08:15 PM (9 years, 7 months ago) |
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Dhearic


Registered: 01/24/14
Posts: 846
Loc: Neverland
Last seen: 1 year, 8 months
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: eatyualive]
#21726375 - 05/26/15 08:19 PM (9 years, 7 months ago) |
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Found me a project for next week. You rock eat!
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Credit where credit is due.
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hamloaf
Q-dood ®©™√



Registered: 12/23/09
Posts: 24,389
Loc: ation: Based.
Last seen: 1 day, 2 hours
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: NumeroEno]
#21731340 - 05/28/15 06:30 AM (9 years, 7 months ago) |
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Quote:
NumeroEno said: Also ham did you get that PM I sent you about the bag pasteurization? I think my method is pretty solid, and I just did some pan cyan sub in a bag last night, but I was wondering if you had anything to add.
Dude yer solid. Just be sure to monitor the internal core temp of EACH of your substrate bags very closely with a meat thermometer, because one thing that can happen when pasteurizing large bags like this is that the bags can, and will heat up at sightly different rates. Solution, as mentioned before, keep a close eye on your substrates' internal core temps. If one bag is increasing in temperature at a faster rate than the other simply turn the saucepan on the stove a full 180 degrees. Monitor your substrates' internal core temps every 20 minutes until you gain a better feel for how your pasteurization equipment will perform.
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Buck513

Registered: 04/17/14
Posts: 5,682
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf]
#21731351 - 05/28/15 06:38 AM (9 years, 7 months ago) |
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I liked you when you were a distinguished and sophisticated gentleman
-------------------- Fail to plan and you plan to fail.
Enter the Ban Lottery
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NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf]
#21731763 - 05/28/15 09:08 AM (9 years, 7 months ago) |
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Quote:
hamloaf said: Dude yer solid. Just be sure to monitor the internal core temp of EACH of your substrate bags very closely with a meat thermometer, because one thing that can happen when pasteurizing large bags like this is that the bags can, and will heat up at sightly different rates. Solution, as mentioned before, keep a close eye on your substrates' internal core temps. If one bag is increasing in temperature at a faster rate than the other simply turn the saucepan on the stove a full 180 degrees. Monitor your substrates' internal core temps every 20 minutes until you gain a better feel for how your pasteurization equipment will perform.
Good call. I've just been leaving the meat thermometer in one bag and setting the alarm. I'll start checking both bags. I can do enough substrate for 2 66 quart monotubs in one run using the bags, so I prefer it over jars for that reason. Anyway, back on topic.
Made this HLC yesterday with a wedge of GT from a plate that was forming primordia. It's probably about 5 transfers away from the original multispore germination.
I made a nice new lid with a whatman and put a shard of glass inside this one. It's also a much less rich solution. I noticed trich floating on top of the last one so it got tossed 
--------------------
Let it grow! Let it grow! Greatly yield!
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in
DOG FOOD AGAR
MY ELECTRIC INOCULATION LOOP
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hamloaf
Q-dood ®©™√



Registered: 12/23/09
Posts: 24,389
Loc: ation: Based.
Last seen: 1 day, 2 hours
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: NumeroEno]
#21731936 - 05/28/15 10:05 AM (9 years, 7 months ago) |
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Ah coolcool. Are you positive that trich was observed floating to the top of your LC, because often times, especially if temps get above 80F, and are sustained, bits/pieces of mushroom mycelium will float to the top, and begin colonizing the top of the media like that.
The solution is to try to split up the myce mat by shaking, stirring magnetically, or flame sterilizing some forceps, and "fishing" the mat of mycelium out of the culture. If the the mat of mycelium that forms on the top of LC's is left unchecked it will suffocate, and kill the live culture below.
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