|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
DeighFry
The Last Baron

Registered: 11/26/14
Posts: 200
Loc: Juniper
Last seen: 7 years, 2 months
|
Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf]
#21653113 - 05/08/15 12:26 PM (9 years, 8 months ago) |
|
|
Quote:
hamloaf said: "Now" brand, grocery store agar agar
What grocery store do you find agar Ham? I've had no luck.
--------------------
Don't fake it baby.
Lay the real thing on me.
Spiraling up through the crack in the skye.
Leaving material world behind.
I see your face in constellations.
The martyr is ending his life for mine.
|
rxb
n00b-sabot



Registered: 08/24/13
Posts: 15,179
Loc: FREE PSYCHONAUTICA
Last seen: 3 months, 27 days
|
Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: DeighFry]
#21653126 - 05/08/15 12:31 PM (9 years, 8 months ago) |
|
|
depends on where you are as to where you find agar.
in alot of cities its in kroger (not mine).
in alot of cities its aparently in walmart says the internets (not mine)
in mine they have it at the vegetarian grocery store, at whole foods, and at the health food store.
-------------------- ->$10 FLOW HOOD ALTERNATIVE <-
. i cleaned a mold contaminated live culture and saved it. (might have useful applications)
[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]
|
DeighFry
The Last Baron

Registered: 11/26/14
Posts: 200
Loc: Juniper
Last seen: 7 years, 2 months
|
Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: rxb]
#21653233 - 05/08/15 12:53 PM (9 years, 8 months ago) |
|
|
Quote:
rxb said: in mine they have it at the vegetarian grocery store, at whole foods, and at the health food store.
I'll have to try more of those then. I tried GNC, no luck.
Thanks.
--------------------
Don't fake it baby.
Lay the real thing on me.
Spiraling up through the crack in the skye.
Leaving material world behind.
I see your face in constellations.
The martyr is ending his life for mine.
|
rxb
n00b-sabot



Registered: 08/24/13
Posts: 15,179
Loc: FREE PSYCHONAUTICA
Last seen: 3 months, 27 days
|
Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: DeighFry]
#21653361 - 05/08/15 01:32 PM (9 years, 8 months ago) |
|
|
yeah i dont mean like gnc.
i mean in stores where they sell everything organic and fair trade but food.
and
in stores where there are no meat products.
hindu groceries.
-------------------- ->$10 FLOW HOOD ALTERNATIVE <-
. i cleaned a mold contaminated live culture and saved it. (might have useful applications)
[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]
|
DeighFry
The Last Baron

Registered: 11/26/14
Posts: 200
Loc: Juniper
Last seen: 7 years, 2 months
|
Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: rxb]
#21653396 - 05/08/15 01:44 PM (9 years, 8 months ago) |
|
|
A hunting I will go.
--------------------
Don't fake it baby.
Lay the real thing on me.
Spiraling up through the crack in the skye.
Leaving material world behind.
I see your face in constellations.
The martyr is ending his life for mine.
|
invitro

Registered: 05/03/13
Posts: 2,529
Last seen: 1 year, 1 month
|
Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf]
#21653648 - 05/08/15 02:48 PM (9 years, 8 months ago) |
|
|
What is meant by "stronger" ?
|
eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
|
Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: invitro] 1
#21654823 - 05/08/15 08:10 PM (9 years, 8 months ago) |
|
|
im going to give that a try very soon! thanks for the pointer.
|
hamloaf
Q-dood ®©™√


Registered: 12/23/09
Posts: 24,389
Loc: ation: Based.
Last seen: 1 day, 2 hours
|
Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: DeighFry]
#21656243 - 05/09/15 07:41 AM (9 years, 8 months ago) |
|
|
Quote:
DeighFry said:
Quote:
hamloaf said: "Now" brand, grocery store agar agar
What grocery store do you find agar Ham? I've had no luck.
"Now" brand agar powder is personally sourced from a local Ma & Pa heath food/organic grocery store. Asian markets have "whole" agar that needs to be ground up. Agar is a seaweed.
Here are a few links for online sources of "Now" brand agar agar. http://www.vitaglo.com/6410.html http://www.nowfoods.com/Agar-Powder-2oz.htm http://www.amazon.com/Foods-Agar-Powder-Ounce-Bottle/dp/B000MGSJ5Ahttp://www.amazon.com/Foods-Agar-Powder-Ounce-Bottle/dp/B0017WEA6K
This is the way to go if you aren't going to use grain water as your nutrient source for agar media. http://www.fungi.com/search-results.html#/?q=agar
|
hamloaf
Q-dood ®©™√


Registered: 12/23/09
Posts: 24,389
Loc: ation: Based.
Last seen: 1 day, 2 hours
|
Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: invitro]
#21656264 - 05/09/15 07:53 AM (9 years, 8 months ago) |
|
|
Quote:
invitro said: What is meant by "stronger" ?
The less you dilute a HLC, at the expense of generating a media that is difficult to impossible to see through, the more luxurious, vibrant, and aggressive the mushroom mycelium becomes on the grain substrate, and I have a few theories as to why. It's understood the role of genetics when it comes to colonization speed, and rigor. It's also understood that media, and growing conditions/environment ALSO play a key roll in speed, and vigor of colonization. That being said, beefing up your HLC with a higher concentration of grain water/nutrient source is a factor of gaining a superior culture due to the type/style of media used.
|
Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,972
Loc: Canada
|
Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf] 1
#21656455 - 05/09/15 09:01 AM (9 years, 8 months ago) |
|
|
The reason I started to play with grain water media was because i was so dissatisfied with the performance of simple sugar LCs. The culture was always weak in terms of recovery after inoculation of grain spawn and the growth within the LC was usually sparse as well. Despite everything that I read telling me that 2% nutes was enough for LC and that simple sugars were fine as a nute source I was frustrated with it every time. Even malt extract (which was a slight improvement over most sugar LC's) was not enough to really make me feel like it was achieving what it should have been.
My first grain water LC was from rye and I was blown away at not only how much faster the culture recovered after inoculation, but also at how thick and aggressive the growth was. I suspected that the rich nutrient state of the inoculating media contributed to the culture becoming aggressive when it was presented with less accessable media in the form of grain.
The kicker is all that nutrition does mean that more care needs to be taken as the soak water is loaded with endospores and in such an environment they can bloom fast. I also find that massive temperature swings during colonization can be detrimental to the grain water LC's health as well. So a longer sterilization cycle and care in storage is helpful. I actually usually sterilize my grain water LC with my grains. That would totally carmelize a sugar LC but its less of a problem for the grain water.
Edited by Pastywhyte (05/09/15 09:09 AM)
|
Tomandjerry58
Stranger

Registered: 01/27/03
Posts: 5,212
|
Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: Pastywhyte]
#21656510 - 05/09/15 09:18 AM (9 years, 8 months ago) |
|
|
Have you guys ever seen anything like this in an lc. Pan cam in dextrose/lme. Kind of looks like fruits and they float
|
hamloaf
Q-dood ®©™√


Registered: 12/23/09
Posts: 24,389
Loc: ation: Based.
Last seen: 1 day, 2 hours
|
Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: Tomandjerry58]
#21656580 - 05/09/15 09:41 AM (9 years, 8 months ago) |
|
|
Whoa, what the heck do you have going on there? Can you get a topical view of the phenomenon please? Also, what's up with all those little circular colonies of mycelium all over? That's not a good sign. Neither is your floating phenomenon as far as can be told from here, currently. Never seen anything like the floating before, no. Are you using magnetic stirring devices, or are you stirring by hand?? Definitely test that culture on a small sterilzed medium such as a plate of agar, pf cake, or a pint jar of grains before using that LC for mass inoculations.
|
directexperience
Broadcastmindfi



Registered: 11/10/14
Posts: 197
Loc: PNW
Last seen: 14 days, 19 hours
|
Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf]
#21656604 - 05/09/15 09:50 AM (9 years, 8 months ago) |
|
|
This is really a wonderful money saving idea
|
Tomandjerry58
Stranger

Registered: 01/27/03
Posts: 5,212
|
Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf]
#21656764 - 05/09/15 10:39 AM (9 years, 8 months ago) |
|
|
Inoculated with a very small wedge and hand stirred every couple of days. The little colonies were seen early on latching onto to the very small undissolved sugar it seemed (I did not filter) .I pulled a syringe out of it and it was viable, then it was left for like 3 weeks with no stirring at all.
I decided to look at it today because my outdoor patch is starting to grow out. But it didn't have these lil growth,floaty things before. When time allows,I will test it but I have a lot of doubt at this point lol
|
PlantSeeker
Natures Grower


Registered: 05/02/15
Posts: 118
|
Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf]
#21662015 - 05/10/15 05:02 PM (9 years, 8 months ago) |
|
|
Keep up the good work. Love reading your posts!
|
eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
|
Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: PlantSeeker] 1
#21684753 - 05/15/15 09:01 PM (9 years, 8 months ago) |
|
|
day 2 after inoculation signs of leap off! now, the LI i did has colonized quite a bit in 4 days. but i used 4 times the amount of LI than LC here. the LC is showing leap off a day faster.
i went extremely light with only 1 cc per quart as i need to delay these jars about a week. all jars are showing leap off. the updated image will more than likely be fully colonized by the time i can check it.

i also used my nice utensil here. i wrapped tip in foil. making sure that the part that opens up is the part that screws on to the syringe body at the bottom of the picture below. i pressure cooked for 20 mins at 15psi. once cool, setup a sab, put all utensils, GWLC, Grain Jars and sterile syringe body still wrapped in the plastic inside the sab. i halfway unwrap the syringe body plastic not exposing the tip yet. i unfold the foil just enough so that when i pull the syringe body out, i immediately place the syringe plastic needle on the body. the rest of the foil is covering the syringe tip until the liquid is to be taken. i lifted the lid of the lc, sucked up the lc. closed the lid. lifted lid of receiving jar, squirted 1cc, then closed the lid. this was one steady motion in a sab. i didn't touch the syringe tip on anything and i did this for 15 jars in a row. I also don't want to melt these syringe tips.
syringe needle
Edited by eatyualive (05/15/15 09:16 PM)
|
hamloaf
Q-dood ®©™√



Registered: 12/23/09
Posts: 24,389
Loc: ation: Based.
Last seen: 1 day, 2 hours
|
Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: eatyualive]
#21685319 - 05/15/15 11:42 PM (9 years, 8 months ago) |
|
|
Excellent work. Great procedure. Superb. Eatyu wins! Flawless Victory! Fatality!
How was it for you busting out all your old LC utensils, and employing/utilizing them again on some LC action?? Ha, bet you were like, "DAYUM, SUN!". 
Can't wait for you to start going around the world with LC's again, so you can begin offering your take on them (LC's). Outstanding Job!
|
eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
|
Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf] 1
#21689858 - 05/17/15 09:25 AM (9 years, 7 months ago) |
|
|
Badass. Bringing back the memories. The syringe tips are eccellent and dont get clogged!
|
hamloaf
Q-dood ®©™√



Registered: 12/23/09
Posts: 24,389
Loc: ation: Based.
Last seen: 1 day, 2 hours
|
Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: eatyualive]
#21689910 - 05/17/15 09:49 AM (9 years, 7 months ago) |
|
|
Cool. Welcome back to the LC kingdom, eatyualive. It's been discovered that with the aid of magnetic stirring devices, syringes becoming clogged due to trying to aspire a large chuck of live culture is an issue of the past.
475cc's of WBS HLC aspired into recycled syringes.
Edited by hamloaf (05/17/15 10:18 AM)
|
DeighFry
The Last Baron

Registered: 11/26/14
Posts: 200
Loc: Juniper
Last seen: 7 years, 2 months
|
Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf]
#21714922 - 05/23/15 05:11 PM (9 years, 7 months ago) |
|
|
hamloaf, how is that grain water agar working out?
--------------------
Don't fake it baby.
Lay the real thing on me.
Spiraling up through the crack in the skye.
Leaving material world behind.
I see your face in constellations.
The martyr is ending his life for mine.
|
|