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Offlineblackout
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf]
    #21639471 - 05/05/15 01:29 PM (9 years, 8 months ago)

Quote:

hamloaf said:
Trying of some wine/beer finings as a liquid media choice would be advocated, and encouraged, blackout.  Good idea.


egg whites can be used to clear wine & beer so I guess it would work with these LCs, you would only need a small bit of egg white for the typical volume of LC we use.

And yes freezing is no doubt the best storage method long term. If people are letting it try and settle to decant it off the fridge is the best bet, as it will not only retard growth, but clear it faster too. I often cap unfiltered jars and put them in a basin of water to cool faster too. You do not want them sitting at typical incubation temps for any length of time.

I was reading on mycoptia that some guy apparently was doing a course in aloha medicinals and apparently they do not boil grains in excess water as they believe straining water off gets rid of water soluble nutrients. They do wash them before processing though. I usually wash mine with washing up liquid and rinse it off. Maybe these water soluble nutrients are what makes these grain LCs so good.

There will also be some naturally occuring sugars in the grain, and I know Workman favours very dilute sugar based LCs, only 0.1-0.5% sugars.

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Invisibleeatyualive
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: blackout]
    #21640225 - 05/05/15 04:47 PM (9 years, 8 months ago)

would you mind updating the op with your stir plate spinning information? example, how long and how many rpms..


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Invisibleeatyualive
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: shroominmyroom] * 1
    #21640230 - 05/05/15 04:47 PM (9 years, 8 months ago)

Quote:

shroominmyroom said:


GWLC is awesome.



Panaeolus Bisporus, seven days since HLC inoculation



nice pics! its almost like a variety of good beer!


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EAT GETS SHIT DONE


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OfflineDhearic
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: eatyualive]
    #21640249 - 05/05/15 04:52 PM (9 years, 8 months ago)

Quote:

eatyualive said:
would you mind updating the op with your stir plate spinning information? example, how long and how many rpms..




This would be very helpful info. I'm gonna use this tek at my next go with LCs, normally I've used either Karo or Honey.

Thanks for the good write up Loaf :thumbup:


--------------------


Credit where credit is due.

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OfflinehamloafM
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: Dhearic]
    #21643451 - 05/06/15 09:34 AM (9 years, 8 months ago)

Went a little hard this morning, and decided to do 1:2 (half strength) grain water to plain water ratio HLC.






Unsterilized end-of-boil-cycle grain water comprised of rye/horse maure/gypsum/spent coffee grounds.

The two quart jar mediums will be inoculated with cube mycelium.  LC generated inside of the large, quart jars will be used to inoculate sterilized bulk subs with hydrated grains inside of spawn bags using LC gun @ 30-40cc's of LC per bag.  The pint sized media will most likely be inoculated with a pre-meditated/random edible wedge generated from LC from vendor, and it's content given away in the market place.  Also, rubber gaskets on each of these new liquid mediums were utilized.

No leaks!


These mediums are in the AA x25 warming up to boiling temps, and are scheduled to be PC'ed for an hour and a half at 15-17 psi.


--------------------
     
How I Get Stuff done. - My Reference Guide. - My Grows.

Edited by hamloaf (05/06/15 10:00 AM)

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OfflineDhearic
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf]
    #21643577 - 05/06/15 10:21 AM (9 years, 8 months ago)

You wouldn't happen to know where to buy those high quality pp5 plastic lids in the wide mouth(90mm) size would you? I bought 10 of the higher quality regular mouth lids to use with LCs and LIs but after hours of searching I've never found a wide mouth equivalent.

I like the construction of the solid regular mouth lids way better than the flimsy shit from wally world that they have for wide mouths.

Using up the last of my jars that I topped with metal lids today. Nothing but plastic for this guy from here on out :laugh: So many torn gloves from trying to wrench open old metal lids....


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Credit where credit is due.

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OfflineDeighFry
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: Dhearic]
    #21643720 - 05/06/15 11:07 AM (9 years, 8 months ago)

@Dhearic

I get all of my plastic wide mouth lids from Wal-Mart & Fred Meyers.

I was doing SAB work, and tried to wrench the pressure-sealed metal lid off.You gotta' wrench those muddas, when the pressure gave, my arm flew up and threw my whole SAB. A whole session, and 3 plates laid to waste.

Plastics are better, I agree.


--------------------
Don't fake it baby.
Lay the real thing on me.

Spiraling up through the crack in the skye.
Leaving material world behind.
I see your face in constellations.
The martyr is ending his life for mine.


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Invisibleblackdust
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf]
    #21643766 - 05/06/15 11:19 AM (9 years, 8 months ago)

this is the type of stuff i make PDF of the page and add them to my notes

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Offlinerxb
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: blackdust]
    #21645944 - 05/06/15 08:20 PM (9 years, 8 months ago)

i wonder if adding sugar/honey/dme etc to the boil phase would help give the myc something to fee on, and increase usefulness of using the boil liquid as a lc media.


--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]

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InvisibleCplCauliflower
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: rxb]
    #21646733 - 05/06/15 10:48 PM (9 years, 8 months ago)

Super interesting shit here. Can't wait to get my May grow log going.

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OfflinehamloafM
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: Dhearic]
    #21646846 - 05/06/15 11:15 PM (9 years, 8 months ago)

Quote:

Dhearic said:
You wouldn't happen to know where to buy those high quality pp5 plastic lids in the wide mouth(90mm) size would you? I bought 10 of the higher quality regular mouth lids to use with LCs and LIs but after hours of searching I've never found a wide mouth equivalent.

I like the construction of the solid regular mouth lids way better than the flimsy shit from wally world that they have for wide mouths.

Using up the last of my jars that I topped with metal lids today. Nothing but plastic for this guy from here on out :laugh: So many torn gloves from trying to wrench open old metal lids....



Nah, I haven't.  If you do let us know, please, and the same will be done on the part of this cultivator.  :super:


--------------------
     
How I Get Stuff done. - My Reference Guide. - My Grows.

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Offlinerxb
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf]
    #21646857 - 05/06/15 11:18 PM (9 years, 8 months ago)



--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]

Edited by rxb (05/06/15 11:19 PM)

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OfflinehamloafM
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: rxb]
    #21646870 - 05/06/15 11:21 PM (9 years, 8 months ago)

Quote:

rxb said:
i wonder if adding sugar/honey/dme etc to the boil phase would help give the myc something to fee on, and increase usefulness of using the boil liquid as a lc media.



Doubt this.  Sugar LC's are found to be inferior to straight LME LC's.  LME LC's become inferior once DEX is added, so I think the same would ring true for the HLC.  If you want additives in your HLC, try throwing shit like manure, gypsum, wheat bran, wheat straw, spent coffee grinds, ect into your boil water at the beginning of the soaking cycle.


--------------------
     
How I Get Stuff done. - My Reference Guide. - My Grows.

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Offlinerxb
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf]
    #21646888 - 05/06/15 11:26 PM (9 years, 8 months ago)

i do gypsum and coffee spent/not doesnt seem to effect it.

never added much else.


--------------------
->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]

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OfflinehamloafM
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: rxb] * 2
    #21646911 - 05/06/15 11:32 PM (9 years, 8 months ago)

Ended up getting those three mediums that were generated earlier out of the half strength rye/horse manure/gypsum/spent coffee gounds HLC sterilized, and inoculated all in one day.  The pint sized medium was inoculated with Grifola Frondosa - Maitake Mushroom agar wedge.  The two quart jar mediums were inoculated with Psilocybe Cubensis agar wedge.  1 jar, Pacifica Exotica Spora Hawaiian (PESH), and the other quart jar, Golden Teacher. 



--------------------
     
How I Get Stuff done. - My Reference Guide. - My Grows.

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Invisibleeatyualive
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf] * 1
    #21650160 - 05/07/15 06:28 PM (9 years, 8 months ago)

here is my hamloaf grain water liquid culture. its a few days in. ive put it to the stir plate twice for about 1 minute bursts on each jar. this was just after the spin.





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OfflinehamloafM
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: eatyualive]
    #21650244 - 05/07/15 06:51 PM (9 years, 8 months ago)

Awesome!  :awesome:  Indeedsir.  Recently I have been stirring my LC's every time the lab is entered which is every 1-2 days.  Pint sized LC's each have an inch long stirbar in them, and have been getting stirred at 700-800rpms for 5-10 minutes at a time.  The reason for the high rpm stir on the pint sized jars is due to the utilization of the 1 inch stirbar.  The 1 inch stirbar needs higher rpms to really break up that culture, so from here on out, the 1 inch stir bar will be reserved for species with fragile mycelium.  With hardier myceliumed species the industry standard stir bar is going to be either the 2 inch stir bars, or the 3.5cm circular stirbar (the shit). 

 

The quart jar LC master that's spread between three threads (WBS HLC) has the 3.5 circular stir bar in it.  The circular stir bar shreds and breaks apart the liquid myce at a much lower rpms than the 1 inch stir bar.  When stirring culture within the quart jar with 3.5cm stir bar, 500-555rpms is used for roughly 5-10 minutes.  A shift in which stir bar to use, and in what sized vessel has been made.  In the new HLC's - 2 inch magnetic stir bars were used in the quart jar, and one of the 3.5 circular stir bars were used in the pint sized jar.  We'll see how this set up works out before deciding to settle on any one method/set up yet.


--------------------
     
How I Get Stuff done. - My Reference Guide. - My Grows.

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Invisibleeatyualive
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf]
    #21650292 - 05/07/15 07:03 PM (9 years, 8 months ago)

i only have one small stir bar. and the other is the long one. so i used what i had. i get quite the tornado going on the larger stir bar in a pint jar.  i only need it to spin for a minute and it works great. i do about the same your doing 1-2 days then stir! once the lc's grow a little more ill up the time for sure. thanks for the info.

on my next lc's im going to go with the darker more concentrated lc. i love the bold color.


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EAT GETS SHIT DONE


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OfflinehamloafM
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: eatyualive] * 1
    #21651725 - 05/08/15 01:46 AM (9 years, 8 months ago)

You will not be disappointing by making a HLC less diluted.  The HLC is STRONGER the less you dilute it with water.  Downside : the less the medium is diluted the murkier/not see through the LC will be.  Depending on who you are, this could be a PITA.  Try telling that to the mushroom mycelium though.  :wink:

Check this out all you myco-voyeurs out there.  Plates of agar poured using the grain water culturing media generation technique.  Media used : 500ml's of half strength end-of-boil-cycle grain water rye/horse manure/gypsum/spent coffee grounds mixed with 10 grams of "Now" brand, grocery store agar agar sterilized at 17-20 psi for 45 minutes. 



--------------------
     
How I Get Stuff done. - My Reference Guide. - My Grows.

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InvisiblePastywhyteMDiscord
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf]
    #21651953 - 05/08/15 04:57 AM (9 years, 8 months ago)

Nice, I love that recipe :smile:

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