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hamloaf
Q-dood ®©™√


Registered: 12/23/09
Posts: 24,389
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Welcome To The HLC! (Hamloaf Liquid CULTURE!) 9
#21627998 - 05/02/15 10:13 PM (9 years, 8 months ago) |
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There's a new breed of liquid culture enthusiasts coming forth from their still air boxes, or sterile air laboratories with a new and improved kind of liquid medium. These brave souls will be from here on out known as the "HLC" - Hamloaf Liquid Culture where we're more than just a passing liquid media phase. We are a CULTURE! Haha, whatever. Let's get started.
Collect your end-of-the-boil-cycle grain water into a container that can be frozen, because the end-of-boil-cycle grain water is best preserved/stored frozen. Plastic containers work great. To thaw the frozen, set it into a sauce pan with jar lid rings on the floor of the sauce pan, fill with water, set your stove to medium, and walk away.

Takes about 30-45 minutes to become fully thawed. The end-of-the-boil-cycle grain water may be used freshly after a boil-cycle also. Warning, contents will very be hot when opting to use freshly boiled water, so use caution.
To create mushroom liquid mycelia medium out of the end-of-the-boil-cycle grain water, simply fill your liquid media vessel of choice up with roughly 1 part end-of-the-boil-cycle grain water : 4 parts plain water, cap, sterilize for 45 minutes at 15-17 psi, let cool, inoculate, and BAM! GWLC. Good luck.






Manure/verm sub

Pasteurized wheat straw substrates.


Straw logs.

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shroominmyroom
Grasshopper


Registered: 10/30/14
Posts: 3,639
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf]
#21628008 - 05/02/15 10:15 PM (9 years, 8 months ago) |
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Edited by shroominmyroom (12/11/17 01:57 AM)
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,916
Loc: Milky way
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: shroominmyroom] 2
#21628019 - 05/02/15 10:17 PM (9 years, 8 months ago) |
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completely liquid media only needs to be sterilized 10-15m but if you're real worried 20. why do you use 45.
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jwalt420
Terpin master


Registered: 04/25/11
Posts: 830
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: shroominmyroom]
#21628029 - 05/02/15 10:20 PM (9 years, 8 months ago) |
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hamloaf
Q-dood ®©™√


Registered: 12/23/09
Posts: 24,389
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: bodhisatta] 1
#21628037 - 05/02/15 10:22 PM (9 years, 8 months ago) |
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Quote:
bodhisatta said: completely liquid media only needs to be sterilized 10-15m but if you're real worried 20. why do you use 45.
As an added measure to extra sterility.
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tripdawg420
low life with no life



Registered: 02/02/09
Posts: 7,653
Loc: illinois
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: jwalt420]
#21628045 - 05/02/15 10:24 PM (9 years, 8 months ago) |
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silverstem
Caps & Stems


Registered: 10/12/13
Posts: 900
Loc: jordan
Last seen: 7 years, 5 months
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf]
#21628054 - 05/02/15 10:27 PM (9 years, 8 months ago) |
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I usually dilute mine more... Just a very transparent brown tint is left. But I hardly use gwlc's anymore... Now its just add water to colonized grain jar shake pull water out put it in sterilized jar.....
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Shroomery needs a gun forum!!!!!!!!! CAN WE HAVE ONE?????
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rxb
n00b-sabot



Registered: 08/24/13
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: tripdawg420]
#21628067 - 05/02/15 10:30 PM (9 years, 8 months ago) |
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thats a wonderful idea.
i wonder what the shelf life would be if you just sterilized in a jar with the standard equipment lid and did not freeze it.
-------------------- ->$10 FLOW HOOD ALTERNATIVE <-
. i cleaned a mold contaminated live culture and saved it. (might have useful applications)
[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]
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DeighFry
The Last Baron

Registered: 11/26/14
Posts: 200
Loc: Juniper
Last seen: 7 years, 2 months
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: rxb]
#21628549 - 05/03/15 01:23 AM (9 years, 8 months ago) |
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Nice!
I've been waiting for a GWLC TEK that had dilution ratio to it.
What ratio do you use of water to grains to achieve the end-of-boil-cycle water content?
--------------------
Don't fake it baby.
Lay the real thing on me.
Spiraling up through the crack in the skye.
Leaving material world behind.
I see your face in constellations.
The martyr is ending his life for mine.
Edited by DeighFry (05/03/15 03:29 AM)
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ForgottenFreshness
Staying High


Registered: 11/16/13
Posts: 211
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: DeighFry]
#21628559 - 05/03/15 01:27 AM (9 years, 8 months ago) |
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lol nevermind...
Edited by ForgottenFreshness (05/03/15 01:28 AM)
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PsyCLown89
Shroomaloomed



Registered: 08/18/14
Posts: 789
Loc: South Africa
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Nice! I saved some grain water a few months back and planned to make some LC with it (sorry, HLC now :P).
I did not freeze them or sterilize them (planned to later on), forgot about them and after a week had nice stuff floating on top and looked super manky. Same happened with some jars of hydrated sorghum, very quickly grew some stuff which was not mycelium 
Perhaps sometime I will get a chance to actually try it!
-------------------- My Trade List - Click ME!! [Updated: 02/05/2015]
"It's all in your head"
"Haste makes waste!"
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hamloaf
Q-dood ®©™√


Registered: 12/23/09
Posts: 24,389
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: DeighFry]
#21628787 - 05/03/15 04:17 AM (9 years, 8 months ago) |
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Quote:
DeighFry said: Nice!
I've been waiting for a GWLC TEK that had dilution ratio to it.
What ratio do you use of water to grains to achieve the end-of-boil-cycle water content?
Nah, nothing too precise like that. Just go about your normal grain prep technique, and instead of throwing away that end-of-the-boil-cycle grain water, collect it, and use it as your liquid media of choice. Fast. Easy. Extremely effective.
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf] 1
#21628857 - 05/03/15 05:27 AM (9 years, 8 months ago) |
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like the gallon jug idea dude!! LC probably best way to do those, i picked up two on recycling day recently from the curb, and just been staring at them, imagining how to use them. as always, rock on with ur bad self brother
-------------------- A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!
The whole is greater than the sum of its parts
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DeighFry
The Last Baron

Registered: 11/26/14
Posts: 200
Loc: Juniper
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf]
#21629761 - 05/03/15 11:49 AM (9 years, 8 months ago) |
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Quote:
hamloaf said:
Quote:
DeighFry said: Nice!
I've been waiting for a GWLC TEK that had dilution ratio to it.
What ratio do you use of water to grains to achieve the end-of-boil-cycle water content?
Nah, nothing too precise like that. Just go about your normal grain prep technique, and instead of throwing away that end-of-the-boil-cycle grain water, collect it, and use it as your liquid media of choice. Fast. Easy. Extremely effective.
Right on.
--------------------
Don't fake it baby.
Lay the real thing on me.
Spiraling up through the crack in the skye.
Leaving material world behind.
I see your face in constellations.
The martyr is ending his life for mine.
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hamloaf
Q-dood ®©™√


Registered: 12/23/09
Posts: 24,389
Loc: ation: Based.
Last seen: 1 day, 2 hours
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: PsyCLown89]
#21629944 - 05/03/15 12:28 PM (9 years, 8 months ago) |
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Quote:
PsyCLown89 said: Nice! I saved some grain water a few months back and planned to make some LC with it (sorry, HLC now :P).
I did not freeze them or sterilize them (planned to later on), forgot about them and after a week had nice stuff floating on top and looked super manky. Same happened with some jars of hydrated sorghum, very quickly grew some stuff which was not mycelium 
Perhaps sometime I will get a chance to actually try it!
Yes. Not freezing your end-of-the-boil-cycle grain water for purpose of long term storage will lead to contamination faster than lightening. Same thing with capped jars of hydrated, unpasteurized casing, and/or bulk substrate materials. Enclosing casing, and/or hydrated bulk sub materials creates anaerobic environments in which bacteria, and mold breed faster than bunnies. No need to hydrate your casing, and/or sub materials up until just before time to pasteurize. Once casing, and/or sub materials are pasteurized it's best to use the materials as soon as they cool to room temps, so plan and schedule accordingly. Good luck!
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NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf] 1
#21629974 - 05/03/15 12:36 PM (9 years, 8 months ago) |
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Wow. I think I'm finally gonna try this. I've been wanting to take another stab at LC and this looks very easy.
   
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Let it grow! Let it grow! Greatly yield!
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in
DOG FOOD AGAR
MY ELECTRIC INOCULATION LOOP
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Buck513

Registered: 04/17/14
Posts: 5,682
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: NumeroEno]
#21630122 - 05/03/15 01:18 PM (9 years, 8 months ago) |
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Nice write up mane. I might try this eventually
-------------------- Fail to plan and you plan to fail.
Enter the Ban Lottery
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blackout


Registered: 07/16/00
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: Buck513] 2
#21630156 - 05/03/15 01:29 PM (9 years, 8 months ago) |
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You could fill those ice cube bags with liquid and take them out as you need them. Defrosting in a microwave would be faster.
I prefer clear LCs and was wondering about using wine/beer finings (clearing agents). I have some grain water at the moment, and as with beer I found it cleared a lot faster in the fridge, and of course would be less prone to contams if settling in the fridge than just sitting in your room.
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hamloaf
Q-dood ®©™√


Registered: 12/23/09
Posts: 24,389
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: blackout]
#21630347 - 05/03/15 02:15 PM (9 years, 8 months ago) |
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Trying of some wine/beer finings as a liquid media choice would be advocated, and encouraged, blackout. Good idea. Your findings posted would be appreciated, as well. 
A bit partial to the golden liquid mediums, personally, due to coming up on trying out Karo, SITR, DEX/LME, and straight LME with straight LME being most powerful liquid media broth generated consecutively. GWLC trumps ALL aforementioned liquid media recipes. Hypothesis as to why grain water liquid cultures do better than their predecessors, consecutively, lies within mycelia being already used to eating, and digesting grain medium before LC ever hits grain.
As far as storage of end-of-the-boil-cycle grain water, yes, storing media in refrigerator would be better plan, than storing media at room temps on a shelf. However, after media is stored in fridge for a long period of time EVENTUALLY contams are going to bloom even in lower temperature ranges of inside refrigerator. For long term storage of end-of-the-boil-cycle grain water it's best to be stored in freezer. Storing end-of-the-boil-cycle grain water in freezer stops whole range of biochemical processes, thus, no life can grow when the media is frozen.
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DeighFry
The Last Baron

Registered: 11/26/14
Posts: 200
Loc: Juniper
Last seen: 7 years, 2 months
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Re: Welcome To The HLC! (Hamloaf Liquid CULTURE!) [Re: hamloaf]
#21630801 - 05/03/15 04:24 PM (9 years, 8 months ago) |
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Quote:
hamloaf said: However, after media is stored in fridge for a long period of time EVENTUALLY contams are going to bloom even in lower temperature ranges of inside refrigerator. For long term storage of end-of-the-boil-cycle grain water it's best to be stored in freezer. Storing end-of-the-boil-cycle grain water in freezer stops whole range of biochemical processes, thus, no life can grow when the media is frozen.
I tried to store rye grass soak and rye soak water in the fridge recently. It took maybe about a week to notice spoilage.
--------------------
Don't fake it baby.
Lay the real thing on me.
Spiraling up through the crack in the skye.
Leaving material world behind.
I see your face in constellations.
The martyr is ending his life for mine.
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