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Offlinetrg64
tribal knowledge

Registered: 04/26/15
Posts: 107
Last seen: 17 days, 11 hours
stem butts method first try
    #21624840 - 05/02/15 04:54 AM (9 years, 8 months ago)

UPDATED 22th of june
P. Cubensis

-------Conclusion so far---------------

* Context:
                  first growing attempt ever
                  mushrooms about to be harvested from a kit
                  straw, cardboard and containers freely available
                  books from Stamets and others
                  lots of ideas to confront to reality
                  deafening overconfidence to get over obviously :tongue2:

* Original idea:
                  propagate stem butts on untreated cardboard
                  try spores on cardboard
                  see if it works
                  if yes try on pasteurized straw
                  later try straw to straw transfer

* Procedure followed:
                  hand washed with daily soap before touching cardboard and straw
                  other than that no special care about hygiene
                  Temp. 68°F
                  !! Pasteurization without pre-soaking !!
                  Not much compaction of the straw :sad:
                  Oxygenation by opening the containers every two or three days.
                  !! all the containers but two received by mistake water from a spray bottle previously containing essential oils !!

* Ascertainements:
                  Nothing with spores on cardboard.
                  Stem butts expanding on cardboard :rolleyes:
                  cardboard spawn expanding on pasteurized straw
                  essential oil bottle bringing contam to a kit and chemicaly burning mycelium making it turn glassy, turning some containers stinky.
                  Long time expanding compared to others.
                  Mycelium having trouble getting inside the straw it seems.
                  Mushrooms growing from that poorly colonized looking like straw.

* Personal conclusions so far:
                  Don't spray with that bottle :grin:
                  Make the growth more easy on cardboard but cutting stem butts in pieces and scattering
                  cut straw real fine
                  pre-soak with lime and bleach
                  pack way tighter
                  allow oxygen exchange trough cotton
                  start BRF to get mushrooms and stem butts
                  start grain to get spawn to do straw log from grain
                  compare straw log from cardboard and from grain
                 

----------------------------

2nd may

so I posted already a photo last week: this one:


I pasteurized the straw, let it cool:


inoculated (nice rhizomorphic mycelium i find):


finished the top part of the sandwich
did some empty yoghourt pots, having some mycelium visible so I can control what is happening


26th may

I just come back from a 3 weeks trip in my truck, I took 2 of the 4 yougourt pots with me:

Here is the whole set-up back together


And I used this to inoculate about 2kg of straw in a bigger box:


31st may

one or two start to smell like feet :-(
the others look and smell still fine..

3rd of june

cobweb mold (in fact not) and branches

5th of june

Going slow and.. unusual due to low substrate density, but no contam..


14th of june

I woke up this morning thinking: I'm gonna try to get get some myc from the straw and transfer it to agar.
WHAT A SURPRISE !!! It is one of the two pots I had submerge in water last week thinking it could humidify the straw in its core better. I knew it was a dunk but I didn't think that the myc was strong enough..



17th of june



did a spore print and two petri dishes of agar clone of this one


the second one


22nd of june

it starts to be funky in there



23rd of june

last pics to conclude this experiment:


all these have their stem butts on cardboard for the next experiment!

Edited by trg64 (06/23/15 01:54 PM)

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InvisiblecArcace-x
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Registered: 10/17/13
Posts: 2,412
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Re: stem butts method on the way [Re: trg64]
    #21624857 - 05/02/15 05:13 AM (9 years, 8 months ago)

cool, what species ur growing?


--------------------


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Offlinepyrojew666
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Registered: 11/18/14
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Re: stem butts method on the way [Re: cArcace-x]
    #21624967 - 05/02/15 06:40 AM (9 years, 8 months ago)

Keep us updatedddd!

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Offlinefares
member!
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Registered: 04/26/15
Posts: 43
Last seen: 9 years, 3 months
Re: stem butts method on the way [Re: pyrojew666]
    #21625150 - 05/02/15 08:17 AM (9 years, 8 months ago)

Try putting a few stem butts Directly in the straws and skip
The cardboard, or even in pasteurized sawdust bag
to make (kit or sawdust spawn)

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Offlinetrg64
tribal knowledge

Registered: 04/26/15
Posts: 107
Last seen: 17 days, 11 hours
Re: stem butts method on the way [Re: fares]
    #21625240 - 05/02/15 09:05 AM (9 years, 8 months ago)

did you try?

I thought about it but I was too hungry :-)
I did it to two flushes of P cubensis, I have 3 others tupperware with cardboard in case these tries are not successful (which I doubt, but let's see)
Like stamets says: move it or lose it, so I have my backup

The cool stuff is that you can start growing with no sterile setup, no tools, just a thermometer, from a kit or a friend giving you a piec of cardboard


--------------------
my stem butt experiment

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Offlinetakofako
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Registered: 01/05/15
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Re: stem butts method on the way [Re: trg64]
    #21625464 - 05/02/15 10:26 AM (9 years, 8 months ago)

please post them into 1 topic so we can monitore it easy.
i am very curious about this experiment :smile: good luck


--------------------
Sorry about spelling, english is not my main language :smile: My Grow Log and My Teks

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Offlinetrg64
tribal knowledge

Registered: 04/26/15
Posts: 107
Last seen: 17 days, 11 hours
Re: stem butts method on the way [Re: takofako]
    #21724972 - 05/26/15 01:56 PM (9 years, 7 months ago)

some new pics..

I was surprised that it grew quite well considering that each box was airtight and opened only 4 ou 5 times in three weeks..

Looking good though:-)

I think I'm gonna improve the set up, put all the boxes with some little holes in a bigger one with water in the bottom, for better oxygen supply and to keep the moisture..

Any good reasons to do otherwise?


--------------------
my stem butt experiment

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Offlinetrg64
tribal knowledge

Registered: 04/26/15
Posts: 107
Last seen: 17 days, 11 hours
Re: stem butts method on the way [Re: trg64]
    #21727483 - 05/27/15 05:31 AM (9 years, 7 months ago)

modified..


--------------------
my stem butt experiment

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Offlinetrg64
tribal knowledge

Registered: 04/26/15
Posts: 107
Last seen: 17 days, 11 hours
Re: stem butts method on the way [Re: trg64]
    #21743591 - 05/31/15 02:08 AM (9 years, 7 months ago)

hmmm... one or two start to smell like feet (but I can't see any color yet)
looks like contams happen on cardboard and straw as well.. (yeah I know, go back to the basics and well known methods :-)


--------------------
my stem butt experiment

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Offlinetrg64
tribal knowledge

Registered: 04/26/15
Posts: 107
Last seen: 17 days, 11 hours
Re: stem butts method first try [Re: trg64]
    #21745740 - 05/31/15 06:23 PM (9 years, 7 months ago)

added a "conclusion so far" section at the beginning of the post for the newbies like me who might be tempted to go offroad with no experience :heart:


--------------------
my stem butt experiment

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Invisibleeatyualive
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Re: stem butts method first try [Re: trg64]
    #21745764 - 05/31/15 06:32 PM (9 years, 7 months ago)

:headbang3:


--------------------
EAT GETS SHIT DONE


:flame::chief:JOIN THE POW WOW:chief::flame:

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InvisibleGrey
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Re: stem butts method first try [Re: eatyualive]
    #21745803 - 05/31/15 06:46 PM (9 years, 7 months ago)

Looks good so far. :rockin:


--------------------


:takingnotes:  AMU Q&A  :takingnotes:


If you don't have a plan of your own, you'll become a part of somebody else's.

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Offlinetrg64
tribal knowledge

Registered: 04/26/15
Posts: 107
Last seen: 17 days, 11 hours
Re: stem butts method first try [Re: Grey]
    #21804861 - 06/14/15 03:22 AM (9 years, 7 months ago)

ha ha ha
I'm so stoked !!
I can't believe it !!!!!!







--------------------
my stem butt experiment

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Offlinetrg64
tribal knowledge

Registered: 04/26/15
Posts: 107
Last seen: 17 days, 11 hours
Re: stem butts method first try [Re: trg64]
    #21806315 - 06/14/15 12:48 PM (9 years, 7 months ago)

it is funny, there are what looks like little rocks of mycelium along the straw, it seems to be everywhere..

The first word that came to my ming was "sclerotia", but I've got no idea what it really is.


--------------------
my stem butt experiment

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InvisibleLocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ
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Re: stem butts method first try [Re: trg64]
    #21806451 - 06/14/15 01:03 PM (9 years, 7 months ago)

Hyphal knots, they are the beginning of primordia ...looks good


--------------------


Q&A
US vs. THEM

The more I learn, the less I know.

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Offlinetrg64
tribal knowledge

Registered: 04/26/15
Posts: 107
Last seen: 17 days, 11 hours
Re: stem butts method first try [Re: LocN9ne]
    #21806635 - 06/14/15 02:07 PM (9 years, 7 months ago)

ah ok, cool :sun:

It motivates me to try again with more compact straw. I really had no concerns about cleanliness, I kept opening them to see, taking the cakes out to see, to smell, to show, handling them with bare hands..

I'm trying the same with oyster here and this time I did the presoaking before pasteurizing and I made it more tight..


--------------------
my stem butt experiment

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Offlinetrg64
tribal knowledge

Registered: 04/26/15
Posts: 107
Last seen: 17 days, 11 hours
Re: stem butts method first try [Re: trg64]
    #21817542 - 06/17/15 02:10 AM (9 years, 6 months ago)

It growing lonely but its growing good :-)


RH is 90%, I'm not sure about FAE, and I'm not sure if I should fan or mist.. Any advise?


--------------------
my stem butt experiment

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Offlinetrg64
tribal knowledge

Registered: 04/26/15
Posts: 107
Last seen: 17 days, 11 hours
Re: stem butts method first try [Re: trg64]
    #21819033 - 06/17/15 12:52 PM (9 years, 6 months ago)

last pic before I clone and spore print this beauty :-)



--------------------
my stem butt experiment

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OfflineShroominMe
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Registered: 05/03/15
Posts: 525
Last seen: 9 years, 2 months
Re: stem butts method first try [Re: trg64]
    #21821696 - 06/18/15 12:33 AM (9 years, 6 months ago)

Quote:

trg64 said:
RH is 90%, I'm not sure about FAE, and I'm not sure if I should fan or mist.. Any advise?



Yes and yes :grin:

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Offlinetrg64
tribal knowledge

Registered: 04/26/15
Posts: 107
Last seen: 17 days, 11 hours
Re: stem butts method first try [Re: ShroominMe]
    #21822192 - 06/18/15 03:10 AM (9 years, 6 months ago)

thank you sir  :takingnotes:


--------------------
my stem butt experiment

Edited by trg64 (06/18/15 03:11 AM)

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