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OfflineEthnobotanicOracle
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Re: No pour agar problems/ condensationnnnn!!!! [Re: Shook.1]
    #21605797 - 04/27/15 07:56 PM (8 years, 9 months ago)

Quote:

Shook.1 said:
hey pasty

sorry to dig up an old thread but i've been looking trying out your pp5 agar tek with mrbarts PDYA recipe and i'm just curious what you or anyone else might think about using a paper towel between the lid and the agar?

similar to how you do yours, but just with an extra couple of folds of paper towel under the lid, then maybe a lil on top like your tek and then wrap the whole thing in foil of course, etc...?
then maybe remove the paper towel in a SAB before inoculation.
or would this just turn into a soggy piece of hell?

also, i know that RR usually recommends using a higher water level in the PC for his liquor bottle pour-agar tek to help the container cool slower and more evenly, so would this maybe help apply to the pp5 condensation situation?
only downside i can see would be that you couldnt stack containers in the PC and therefore couldnt do as many dishes, but for agar beginners...with less-than-perfect contam-free areas, no flowhoods, etc...who may not need a lot of agar to begin with, and would be more worried about condensation-contams...would this be plausible?

just curious...
thanks for humoring me.




I'm sorry i have minimal input, but I'm very curious to see some seasoned members responses to this idea.

Part of me wants to say that it would be begging for contamination, but part of me REALLY loves the idea of being able to take good pictures of the growing mycellium, not the fucking water droplets on the lid...


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OfflineShook.1
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Re: No pour agar problems/ condensationnnnn!!!! [Re: EthnobotanicOracle]
    #21606865 - 04/27/15 11:40 PM (8 years, 9 months ago)

well, my thinking was that if you folded a few layers between the lid and container and then secure it all in foil, PCook it, then i was thinking maybe remove it just as soon as its cooled and ready to use. which might at least minimize the initial condensation and possibly keep folks from having to shake out the water in the SAB...because the air ain't all that "still" if youre having to fumble around with it like that.

By no means am I suggesting LEAVING the papertowel there for very long...i can think of an assload of problems from leaving it there (contams, wicking away moisture, etc).

I just figure the papertowel would get sterilized all the same anyway...I just wonder if it would help with condensation during the temp differential...or would the agar be so incredibly steamy during sterilization that it wouldn't make any real difference...

Just wondered if anyone's tried.

I'm working on a batch tonight and maybe I'll do a couple of extras that I dont mind ruining and see if there's a difference. I'll try and report my results in the next couple of days.


--------------------
“A hallucination is a species of reality, as capable of teaching you as a videotape about Kilimanjaro or anything else that falls through your life.”
Terence McKenna


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InvisiblePastywhyteMDiscord
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Registered: 09/15/12
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Re: No pour agar problems/ condensationnnnn!!!! [Re: Shook.1]
    #21607542 - 04/28/15 04:58 AM (8 years, 9 months ago)

To be honest i dont think it will help. The paper towel under the lid is just going to get soaked with agar. There are lots of ways to deal with condensation, which is not really a big deal anyway. Its not a vector for contams and is more of an annoyance. Just use a little less agar, and wait a few days. it will clear up.


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