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Offlinedmtcorey
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liquid on agar
    #21594596 - 04/25/15 11:10 AM (9 years, 25 days ago)

hey guys, yesterday I done up my first batch of agar and after I inoculated 4 jars with a syringe and placed a piece of colonizing grain on my other 4 jars I realized there was a small amount of liquid in the jars when I slanted them to one side !

im wondering what caused this and im also wondering if they will pull through alright ,im thinking the grain one will be alright but im not to sure about the ones I shot up .

any feed back wound be great ,thanks

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InvisiblePirateSwazey
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Re: liquid on agar [Re: dmtcorey]
    #21594792 - 04/25/15 12:02 PM (9 years, 25 days ago)

Sounds like condensation from the lid fell on the surface of the agar.


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Offlinedmtcorey
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Re: liquid on agar [Re: PirateSwazey]
    #21594830 - 04/25/15 12:14 PM (9 years, 25 days ago)

that's what had passed through my mind , how could I deal with this ?

I mixed 10g of agar ,10g of potato flakes 2/3 tea spoon of honey and 500ml of water ..

is this to much water ?

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Offlinetripdawg420
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Re: liquid on agar [Re: dmtcorey]
    #21594846 - 04/25/15 12:17 PM (9 years, 25 days ago)

condensation will go away after a few days
did you dissolve it before you pc them


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epic GT mono tub
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wbs tek
http://www.shroomery.org/forums/showflat.php/Number/11525679
coir tek
http://www.shroomery.org/forums/showflat.php/Number/11917410
results :thumbup:

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InvisiblePirateSwazey
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Re: liquid on agar [Re: dmtcorey]
    #21594847 - 04/25/15 12:18 PM (9 years, 25 days ago)

Maybe try adding a little more agar to your mix? That or a little less water.

Also stacking your plates helps keep the condensation down while they set.


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Offlinedmtcorey
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Re: liquid on agar [Re: PirateSwazey]
    #21595407 - 04/25/15 02:31 PM (9 years, 25 days ago)

Quote:

tripdawg420 said:
condensation will go away after a few days
did you dissolve it before you pc them



I mixed it up to what I thought was thro,
Quote:

PirateSwazey said:
Maybe try adding a little more agar to your mix? That or a little less water.

Also stacking your plates helps keep the condensation down while they set.



next time I will play with the ratios

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Offlinetripdawg420
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Re: liquid on agar [Re: dmtcorey]
    #21596452 - 04/25/15 07:12 PM (9 years, 25 days ago)

ya after you mix it bring the temp up slowly till it dissolves in the pan :thumbup:


--------------------
HUSTLER
How U Survive This Life Everyday Resourcefully
epic GT mono tub
http://www.shroomery.org/forums/showflat.php/Number/17277772

wbs tek
http://www.shroomery.org/forums/showflat.php/Number/11525679
coir tek
http://www.shroomery.org/forums/showflat.php/Number/11917410
results :thumbup:

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Offlineinvitro

Registered: 05/03/13
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Re: liquid on agar [Re: dmtcorey]
    #21596627 - 04/25/15 07:58 PM (9 years, 25 days ago)

Some people flip their agar upside down so the condensation doesn't accumulate on the agar.

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Offlinedmtcorey
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Re: liquid on agar [Re: tripdawg420]
    #21648842 - 05/07/15 11:39 AM (9 years, 13 days ago)

cool brother man

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InvisibleMad Season
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Re: liquid on agar [Re: dmtcorey]
    #21648859 - 05/07/15 11:45 AM (9 years, 13 days ago)

I get standing water on my agar and it causes no problems. Spores actually germinate better in a wet area. It's actually a practice to drop drops of sterile water on top of dry spores on agar from a print that's sat too long. Mycelium colonizes through it well. It actually seems pretty hard to mess up with agar.


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InvisibleMad Season
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Re: liquid on agar [Re: invitro]
    #21648864 - 05/07/15 11:46 AM (9 years, 13 days ago)

Quote:

invitro said:
Some people flip their agar upside down so the condensation doesn't accumulate on the agar.



Don't do this. Not for our purposes. It'll dry out too quickly. It's fine for a lab with cultures for a week but we can not see spores germ for a month sometimes. We can also store in the fridge for a long time too.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.

Edited by Mad Season (05/07/15 11:47 AM)

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Offlinedmtcorey
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Re: liquid on agar [Re: Mad Season]
    #21650406 - 05/07/15 07:32 PM (9 years, 13 days ago)

I had noticed the mycelium just ate it all up

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